Objective: To study the mental health status and influential factors of elderly people in the rural areas. Methods: 714 elderly people were tested by the SCL-90. Results: All the factor scores of SCL-90 of the elderly people in the rural areas were higher than those of the retired people. Some factor scores of SCL-90 of elderly people showed significant differences because of existence of spouse, physical health, filial piety of sons and daughters, and economic independence. Conclusion: The mental health status of elderly people in the rural areas is poorer than that of retired people. Existence of spouse, physical health, filial piety of sons and daughters and economic independence all influence the mental health of elderly people in the rural areas.

Objective To discuss clinical application of theory of TCM constitution on non-alcoholic fatty liver disease (NAFLD).Methods Literature of nearly 15 years was summarized to analyze the development of Chinese constitution theory and its application of on typing and treatment of NAFLD.Results Main types of constitution in patients with NAFLD were phlegm-dampness,deficiency of Qi and damp-heat.On tiis basis,treatment based syndrome differentiation of TCM could effectively prevent and treat NAFLD.Conclusion TCM constitution theory provide research ideas for NAFLD.

Objective To compare the efficacies of laparoscopic colorectal resection and conventional open surgery by collecting and investigating related studies with Meta-analysis.Methods Randomized controlled trials(RCT) on the outcomes of laparoscopic colorectal resection and conventional open surgery that published between January 1989 and March 2008 in English were collected and analyzed.The quality of the original investigations was evaluated,and the low-level reports were excluded.Then the odds ratio(OR) for the incidence of early postoperative small bowel obstruction between the two methods were calculated.ResultsTotally 12 studies including 3032 cases were analyzed.Among which,1522 patients received laparoscopy,33 of them developed early postoperative small bowel obstruction.1510 cases underwent open surgery,and 71 of these patients showed small bower obstruction before being discharged form hospital.The pooled OR was 0.46 with a 95% confidence interval of(0.30-0.69).Conclusion Compared with open surgery,laparoscopic colorectal resection is superior in the incidence of early postoperative small bowel obstruction.

Objective To contrastively analyze the performance of two kinds of blood cell separators for apheresis platelets . Methods 80 blood donors were selected from 4 234 healthy apheresis platelet donors and their data before and after apheresis platelets were respectively collected by using two kinds of different blood cell separators Trima and Amicus .The product quality during the collection process ,residual WBC and RBC count ,acquisition time ,collection efficiency ,scrapping situation and safety were performed the comparative analysis between the two kinds of blood cell separator .Results In the case of the difference of the basic parameters before apheresis platelet having no statistical significance ,the two kinds of blood cell separator had no differences in the aspects of product quality ,processing blood amount and the use amount of anticoagulants(P>0 .05);but there were statisti‐cal differences in the aspects of acquisition time ,collection efficiency and pipeline residual blood amount (P<0 .05) .Conclusion Two kinds of blood cell separators have their own advantages and disadvantages during the use process ,so the blood cell separator should be selected according to the physical quality and their own characteristics of blood donors .

Objective To investigate the feasibility and clinical results under laparoscopic resection of colorectal cancer.Methods Clinical data of 17 colorectal cancer patients with laparoscopic resection from October 2009 to September 2011 were analyzed.Results There were 16 cases of successful completion of laparoscopic surgery,conversion to open surgery in 1 case.Postoperative of incomplete intestinal obstruction and rectal vaginal fistula are each 1 case.Oncology did not occur metastasis through puncture holes in postoperative follow-up up to 2 years.Conclusion Laparoscopic resection of colorectal cancer should be widely applied,and it had more advantages such as less trauma,safety,recover quickly,consistent with the effect of laparotomy.

Objective To investigate the effects of constraint-induced movement therapy (CIMT) on the function of hemiplegic upper extremity in the early subacute stroke patients.Methods A prospective,single-blinded,randomized controlled study was conducted.Forty-seven stroke patients with hemiplegic upper limb dysfunction were randomly divided into 2 groups:a CIMT group and a control group.The CIMT group received constraintinduced movement therapy and the control group was treated with conventional rehabilitation therapy.Both groups were treated 3 h daily,5 d a week for 2 weeks.The Wolf motor function test (WMFT) and Fugl-Meyer Assessment (FMA) were used to evaluate upper extremity motor function,and motor activity log (MAL) was used to assess upper extremity ability in activities.WMFT,FMA and MAL were measured before and after 1 day and 6 months of treatment.Results After 1 day of treatment,FMA,WMFT,MAL-AU and MAL-HW were 53.81 ± 2.59,66.68 ± 3.54,1.89 ± 0.88 and 3.26 ± 0.65,respectively,in the CIMT group,and 48.61 ± 4.48,62.10 ± 7.97,1.25 ± 0.64 and 2.65 ± 0.93,respectively,in the control group.After 6 months of treatment,FMA,WMFT,MAL-AU and MAL-HW were 57.53 ±2.01,69.57 ± 3.00,3.00 ±0.82 and 3.84 ±0.69,respectively,in the CIMT group,and 53.30 ± 2.88,66.20 ±3.59,2.20 ± 1.06 and 3.25 ±0.64,respectively,in the control group.The scores of FMA,WMFT,MAL in the CIMT group were all higher than those in the control group at 1 day and 6 months post-treatment,and the differences were statistically significant (P ＜ 0.05).Conclusion Constraint-induced movement therapy can significantly improve the patients' hand function in the early stage of subacute stroke,which maintain up to 6 months of follow-up.

Objective To investigate the clinical and pathological diagnosis of cutaneous calcifying epithelioma.Methods Thirty cases of calcified epithelioma were diagnosed and treated by our hospital.The clinical features and pathological features were retrospectively reviewed.Results The main pathological features of the tumor were that the main tumor (basophilic cells) basal cells and shadow cells were mixed composition,combined with ossification and calcification and with no recurrence after surgical resection.Conclusion The clinical manifestations of calcifying epithelium are lack of characteristics and diverse pathological features.It is necessary to make the correct pathological diagnosis with the time,location and imaging of tumor growth.

Objective To examine the impact of diabetic foot patients′ negative emotion on the caregiver′quality of life. Methods Totally 100 pairs of diabetic foot patients and their caregivers were investigated using convenience sampling method. Results The incidence of anxiety, depression of hospitalized patients with diabetic foot was 41.5% (23/200), 44.0% (88/200) respectively. Pearson correlation analysis showed that anxiety score were negatively correlated with caregivers′ quality of life except for mental health dimension, physical pain dimension and the total score of physical health, mental health and the MOS item Short from Health Survey (SF-36) (r=-0.471--0.117, P<0.05), and depression score were negatively correlated with caregivers′ quality of life except for physical pain dimension and the total score of physical health, mental health and SF-36(r=-0.519--0.220, P<0.05). Multiple regression analysis indicated that caregivers educational level, provided support, social support , relationship with patients, self-evaluation of health, live together time with patient, patient care burden, caregivers gender, depression score, patient age, diabetic foot Wagner grade were the influence factors of the caregiver′ quality of life. Conclusions Diabetic foot patients′ negative emotion has an important impact on caregiver′quality of life, we can improve the quality of life of patients and their caregivers by reducing the negative mood of patients with diabetic foot.

Objective To evaluate the effects of apolipoprotein-J(ApoJ)on hypoxia/reoxygenation(H/R)induced neonatal rat ventricular cells(NRVCs)injury and its related signaling pathways.Methods ApoJ high expression was achieved by infection with recombinant adenovirus in NRVCs.The three gas incubator was used to establish H/R model,SOD mimetic Mn(Ⅲ) tetrakis(4-benzoic acid)porphyrin chloride and eIF2α dephosphory inhibitor Salubrinal were performed the pretreatment.The NRVCs were divided into four groups:normal control group,H/R,ApoJ group,ApoJ+ H/R group,Mn(Ⅲ)TBAP+H/R group and Salubrinal +-H/R group.The cell viability was measured by MTT assay;the leakages of LDH,the expression of SOD and caspase-3/7 activity were detected by ELISA.The protein expression levels of ApoJ,Nox2/gp91phox,caspase-12,CHOP,and the phosphorylation level of eukaryotic initiation factor 2α(eIF2α)were determined by Western blot.Results ApoJ protein in myocardial cells was highly expressed after infection by recombinant adenoviru.Compared with the control group,the cell viability and the activity of SOD were significantly decreased,the leakages of LDH and the activity of caspase-3/7 were increased in the H/R group,the pro tein expression level of Nox2/gp91phox,caspase-12 and CHOP and the phosphorylation level of eIF2α were increased.Compared with the H/R group,the leakages of LDH and the activity of caspase-3/7 in the ApoJ overexpression group,Mn(Ⅲ)TBAP group and Salubirnal group were significantly decreased.ApoJ overexpression significantly increased the cell viability and the activity of SOD.Moreover,the protein expression level of Nox2/gp91phox,caspase-12 and CHOP were significantly decreased,while the phosphorylation level of eIF2α was markedly increased.Conclusion ApoJ alleviates H/R induced myocardial cellular injury by antioxidative stress and endoplasmic reticulum stress.

Objective To investigate The effect of Heat shock protein 70(HSP70) antisense oligonucleotides (ASO)to bladder carcinoma in mouse loaded with tumor.Methods The 40 mice loaded with tumor subcutaneously were established by cultured BIU-87 cells,and divided into 4 groups randomly when the subcutaneous neoplasms grew to about 100 mm3,namely,HSP70 mRNA ASO plus mitomycin C(MMC)group;HSP70 mRNA ASO group;MMC and blank control.HSP70 mRNA ASO were injected into neoplasms,10mmg/kg weight,twice every week,and MMC 0.1mg/kg weight,twice every week,and the above schemes were replaced with normal saline to blank.The neoplasms were peeled off,photograghed and weighed in 30 days.HSP70 expressions were examined with reverse transcription polymerase chain reaction (RT-PCR),mierovaseular density(MVD)was evaluated by immunohis to chemical staining and the tumor cells apoptosis was detected by terrainal deoxynucleotidyl transferase(TdT)-mediated dUTP-biotin nick end labeling technique (TUNEL).Results The tumor inhibition rate in ASO+MMC surpassed 50%.more than ASO or MMC respectively,and the differences were significantly(P＜0.05).The ASO and MMC exceeded blank group respectively(P＜0.05).The ASO was the same as the MMC(P＞0.05).The apoptotic index(AI)in ASO+MMC surpassed the other three groups (P＜0.05).The difference between ASO and MMC was not significant (P＞0.05),while the A1 of ASO or MMC was more than blank respectively(P＜0.05).The results of MVD were in accordance with the above results.Conclusion The injection of HSP70 mRNA ASO in tumor locally can inhibit neoplasm growth,and this effect might correlate with the inhibition of apoptosis and microvascular forming resulting from the ASO.