Object To study on the structure of sapogenins from pod of Gleditsia sinensis Lam Methods Pod of G. sinensis was extracted with solvents and the constituents in the extraction isolated, after hydrolised completely, with the aid of flash chromatography to obtain 2 gledgenins Results The structures of the 2 gledgenins were proved to be 3 hydroxy 12 oleanen 28 oic acid and 3, 16 dihydroxy 12 oleanen 28 oic acid respectively as shown by IR, NMR, MS and crystallographic data The structures were further verified by acylation, methylation and followed by reaction with 3, 5 dinitro benzoyl chloride Conclusion New crystalline derivatives were obtained to provide further evidence of their structures

Objective To establish the analytical method for the fingerprint of Flos Chrysanthemi by HPLC and estimate the quality of Flos Chrysanthemi in various species from different habitats. Methods The gradient elution mode was applied in chromatographic separation,data can be treated by competitive layer neural network(NN) and pattern recognition be made for Flos Chrysanthemi samples of various species from different habitats.Results The analytical method of fingerprint for Flos chrysanthemi by HPLC was established,samples can be classified into five categories according to the recognition result.Conclusion The established fingerprint can be used for the identification and quality control of Flos Chrysanthemi.

Plasma quinolone concentrations are not routinely measured in clinical practice.However,in order to optimize quinolone treatment,monitoring of plasma concentrations could sometimes be useful particularly in critically ill patients.In this study,anti-sparfloxacin antibody was obtained by immunizing rabbits with sparfloxacin conjugated with bovine serum albumin using isobutyl chloroformate method.After the assay procedure was optimized,the standard curve of sparfloxacin was established.The practical measuring range of the competitive ELISA extended from 5 ng/mL to 2 μtg/mL.The recovery rates and coefficients of variation for rat plasma,urine and tissues were 87.7-106.2％ and 4.8-15.3％,respectively.To demonstrate the potential of the ELISA,a preliminary pharmacokinetics and tissue distribution study of sparfloxacin in rats and quantitative analysis of sparfloxacin in several pharmaceuticals were performed and compared with high-performance liquid chromatography (HPLC).The experimental data indicated that the proposed method would be a valuable tool in therapeutic drug monitoring (TDM) for sparfloxacin.

OBJECTIVE To prepare lipid-coated mesoporous silica nanoparticles （CLMSNs） co-loaded with doxorubicin （DOX） and siRNA （CLMSNs-SS-NH2@DOX/siRNA），and to characterize it and study anti-multidrug-resistant tumor cells. METHODS MSNs-SS-NH2@DOX was prepared on the basis of mesoporous silica （MSNs），covered with cationic liposomes （CLs） to synthesize CLMSNs-SS-NH2@DOX，and then obtain CLMSNs-SS-NH2@DOX/siRNA by co-loading with siRNA. The particle size and Zeta potential of the preparation were determined，and its micromorphology was observed； differential scanning calorimetry，X-ray diffraction，infrared spectroscopy and physical adsorption analysis were conducted. The in vitro release of DOX from the preparation was determined under different pH conditions （pH5.0，pH7.4） and different glutathione concentrations （0，2，5， 10 mmol/L）. The effects of this preparation on the uptake，migration，apoptosis，cycle and P-glycoprotein （P-gp） expression of MCF-7/ ADR in DOX-resistant breast cancer cells were investigated. RESULTS CLMSNs-SS-NH2@DOX/siRNA had a clear core-shell structure，obvious lipid membrane layer，particle size of （197.63±3.75） nm，Zeta potential of （20.64±0.98） mV，and with good physical and chemical properties. In vitro release results showed that CLMSNs-SS-NH2@DOX/siRNA possessed good pH/reduction double-response. The results of cell experiment showed that after intervened with CLMSNs-SS-NH2@DOX/siRNA，the fluorescence intensity of MCF-7/ADR cells was significantly enhanced，the migration rate and P-gp expression level were significantly reduced， while total proportion of apoptosis and that of G0/G1 phase were significantly increased （P＜0.05）. CONCLUSIONS In this study， DOX and siRNA co-loaded CLMSNs-SS-NH2@DOX/siRNA is prepared successfully， which has good physical and chemical properties， pH/reduction double-response properties. It can reverse the multidrug resistance of MCF-7/ADR cells by down-regulation of P-gp expression.