OBJECTIVE:To discuss the clinical features and the general pattern of drug-induced hepatic lesions.METHO-DS:The clinical data of 170 drug-induced hepatic lesion cases collected from Jan.2002 to Apr.2006 in our hospital were analyzed retrospectively.RESULTS:Of the 170 cases,35.29% were induced by Chinese herbal medicines,13.53% by antipsychotic drugs,12.94% by anti-tuberculosis agent;51.18% were hepatocellular damage type,14.12% cholestatic damage type and 34.71% damage of mixed type damage.CONCLUSION:Hepatic lesions resemble viral hepatitis and were of no specificity in clinical features;various drugs can lead to hepatic lesions and to which clinic should attach great importance.

Objective To observe ocular manifestations of acquired immunodeficiency syndrome (AIDS). Methods Fourty-two AIDS patients (66 eyes)with ocular complaints received examinations of visual acuity, slit-lamp microscope, ophthalmoscope and fundus fluorescence angiography (FFA). The results were retrospectively analyzed. ResultsThere are five types of ocular findings, including cytomegalovirus (CMV) retinitis (37 eyes, 56.0%), retinal microvasculopathy of human immunodeficiency virus (21 eyes, 32.0% ), optic nerve diseases (three eyes, 4.5 % ), retinal neuroepithelial layer detachment (two eyes, 3.0%) and uveitis (three eyes, 4.5%). Conclusions The common ocular manifestations showed progressive necrotic retinitis, retinal hemorrhage and retinal vasculitis and attenuated, cotton-wool spots in AIDS patients.

Objective To study the influence of different dosages of atorvastatin in acute coronary syndrome female patients on vascular endothelial growth factor(VEGF) and brain natriuretic peptide(BNP).Methods 60 female cases of ACS patients were divided into A and B group.30 patients in the A group were given atorvastatin 20mg per night,while the other 30 patients in the B group were taken 40mg per night.The patients were followed four weeks by evaluating serum VEGF and BNP concentration.Results After treatment for four weeks,the patients in the two groups showed significant reduction in VEGF and BNP,however,the therapeutic group showed significant difference between the two groups.Additionally,the B group further showed significant reduction in VEGF and BNP.Conclusion Using high-dose atorvastatin lowers VEGF and BNP significantly,which could reduce female cardiovascμlar events.

The morbidity and mortality of cardiovascular diseases(CVD)are still high in our country,they severely threaten peopleS health and quality of life.A dysregulated or reduced autophagy is associated with ischemic heart disease,cardiac hypertrophy,heart failure and arrhythmia.Autophagy is a key regulator in CVD development,and is essential for maintenance of heart function and improvement of prognosis.Thus,modulating autophagy represents an attractive future therapeutic target for treating cardiovascular disease.This article reviewed the research progression about effects of autophagy on ischemic heart disease,cardiac hypertrophy,heart failure and arrhythmia.

OBJECTIVE To give a general introduction to the infection in the hospital,and supervision to key departments in order to prevent nosocomial infection occurrence. METHODS A vertical analysis was made on the basis of the survey involving the following three periods: the first period covered the second half of the year 2000(from Jul to Dec 2000),the second period covered the second half of the year 2002(from Jul to Dec 2002),and the third period covered the second half of the year 2004(from Jul to Dec 2004). RESULTS The periodical supervision and the vertical analysis in the same period showed the following: the rates of the infected patients in the departments showing a tend to go on rising from 2000 to 2002,and showing a tend to decline from 2002 to 2004. CONCLUSIONS The control of using antibiotics must be emphasized and they should be applied in a scientific and reasonable way.The key points include the management of purchasing drugs,clinical indications and usages,supervise and news feedback,etc.All of these measures could prevent the occurrence of nosocomial infection effectively.

The research of interleukin-13 receptor ?2(IL-13R?2) chain is a rising pop these years.Previous studies have shown that many human tumors overexpress IL-13R?2 chain,while normal cells do not express this receptor or express very low level.This difference of express level is significant to diagnose and cure tumors by the IL-13R?2-directed toxin fusion protein.During the past decade,the structure and the function of IL-13R?2 together with the relationship between this receptor and tumors has been further developed.Therefore new therapies and theories can be proposed as the clinical tumor treatments.In this case,the expression in various tumor cell lines was not only focused on but also on the IL-13 and toxin fusion protein killing effect in both the cell level and the in vivo level.Besides,an overview of the mechanism in the treatment of IL-13R?2-directed toxin fusion protein together with the improved methods for fusion protein purification and other relative tumor therapy was given.In conclusion,the current status and progress of IL-13R?2 as well as IL-13R?2-directed toxin fusion protein in tumor therapy were represented.

BACKGROUND: Direct observation of infarcted microvaseular perfusion after cerebral infarction is the most immediate evidence in evaluation of therapeutic effects.OBJECTIVE: To observe dynamic changes in flowing quality and field in cerebral ischemic area in rats so as to qualitatively and quantitatively analyze the microvascular state in pia matral encephali ischemic area and the changes of associated leukocytic flowing velocity and flowing morphology.DESIGN: Randomized controlled experiment was designed.SETTING: Department of Acupuncture and Moxibustion of General Hospital Chinese PLA.MATERIALS: The experiment was performed in Research Room of Microcirculation of General Hospital Chinese PLA, in which, 120 female Wis tar rats of 2-month old were employed and randomized into acupuncture group (36 rats), model group (36 rats), sham-operation group (36 rats) and normal group (12 rats). The first 3 groups were subdivided in 3 hours, 6hours and 24 hours groups according to the prescriptive time divisions, 12rats in each group.METHODS: In acupuncture and model groups, after abdominal anesthesia with pentobarbital sodium, the cranium was opened and the occlusion of meddle cerebral artery was performed in cerebrum with heat-coagulation method. In sham-operation group, anesthesia and cranium opening were performed, but the meddle cerebral artery was not occluded. In normal group, no any management was performed. In acupuncture group, 60 minutes after occlusion of meddle cerebral artery in cerebrum, needling was given on Renzhong (GV 26) and Neiguan (PC 6) with electric stimulation of disperse-dense wave, 4-10 Hz frequency and 0.4 V of intensity, lasting for 5 minutes. Afterwards, pecking-acupuncture technique was done on Renzhong (GV 26) to achieve strong stimulation for 10 seconds. In shamoperation and model groups, the rats were in process of grasping and fixation, but acupuncture was not applied. Vascular endotheliocyte fluorescence staining and leukocyte fluorescence tracing method were applied, in combination with microscopic video system and computer imaging analyzing system, to observe in dynamic and quantitatively the influences of acupuncture on pia matral encephali microvascular morphology, density and blood flowing velocity at 3 hours, 6 hours and 24 hours after cerebral occlusion of meddle cerebral artery.morphology, density and blood flowing state at various time divisions observed directly microscopically and vascular endotheliocyte morphology and the state of brain tissue which near the microvascular after infusion with velocity in infarcted area at various time divisions measured in quantity in each group.microvascular endotheliocytes were colored worse and more fluorescent exudation appeared. Those were better remarkably in acupuncture group ty: That in model group was lower than normal group. That at 3 hours, 6hours and 24 hours in acupuncture group was higher remarkably than model group [(6.92±0.42), (3.25±0.52) pces/visual field; (7.61 ±0.51),(3.68±0.32) pces/visual field; (8.24±0.72), (5.49±1.2) pces/visual leukocyte velocity: That in model group was lower remarkably in normal group. That at 3 hours, 6 hours and 24 hours in acupuncture group was higher remarkably than model group [(1 193±358), (600±261) μm/s;(1 112±267), (517±115) μm/s, (1 766±293), (611±291) μm/s, q=4.608.28, P ＜ 0.01].CONCLUSION: At early stage of cerebral infarction, broken blood flow is severe in artery, vein and capillary in ischemic area; the numbers of opened vessels are few, flow rate is slow down and cerebral perfusion is unsatisfactory. Acupuncture amaliorate the microcirculation perfusion state in ischemic area, increases the numbers of opened vessels and improves flowing morphology of blood cell.

Objective To evaluate the effect of sleep deprivation on cognitive function in the rats undergoing propofol anesthesia.Methods Sixty healthy male Sprague Dawley rats,aged 14-18 weeks,weighing 200-250 g,were randomly assigned into 3 groups (n=20 each) using a random number table:control group (group C),propofol anesthesia group (group P),and sleep deprivation + propofol anesthesia group (group SDP).Propofol was given as a bolus of 15 mg/kg followed by an infusion of 40 mg · kg-1 · h-1 for 2 h in group P.After the rats were subjected to rapid eye movement sleep deprivation for 24 h,the rats received propofol anesthesia in group SDP.Before sleep deprivation,after sleep deprivation,and at 1,3 and 7 days after anesthesia,Morris water maze test was used to assess the learning and memory function,and the escape latency and frequency of crossing the original platform were recorded.Ten rats randomly selected from each group at 1 and 7 days after anesthesia were sacrificed,and brains were removed to observe the morphology of nerve cells in the hippocampal CA1 region (by Nissl's staining) and to detect the expression of phosphorylated Tau at Thr231 (Tau-pThr231) in the hippocampal CA1 region (by immunohistochemisty).Results Compared with group C,the escape latency was significantly prolonged,the frequency of crossing the original platform was decreased,the expression of Tau-pThr231 in the hippocampal CA1 region was up-regulated at 1 day after anesthesia in P and SDP groups (P＜0.05),especially in group SDP (P＜0.05),and there was no significant difference between the groups at the other time points (P＞0.05).The pathological changes were aggravated at 1 day after anesthesia in group SDP compared with group P,and there was no significant difference at 3 and 7 days after anesthesia between group SDP and group P.Conclusion Sleep deprivation can aggravate the transient cognitive dysfunction after propofol anesthesia,and the mechanism is related to promotion of Tau phosphorylation in the rats.

Objective To investigate the therapeutic effect of Danhong injection combined with metoprolol tartrate tablets on cardiac arrhythmia in climacteric women.Methods Retrospective a total of 115 patients with arrhythmia in The First People’s Hospital of Guiyang from May 2014 to May 2016 were divided into the western medicine treatment group and the traditional Chinese and western medicine treatment group.Western medicine treatment group were treated by metoprolol tartrate tablets, the traditional Chinese and western medicine treatment group were treated by Danhong injection combined with metoprolol tartrate tablets.The effect of two groups of patients after two months of treatment were observed,the heart rate variability,heart function index,sleep quality and the incidence of adverse reaction rate were compared between two groups of patients before and after treatment .Results After treatment,the effective rate of the traditional Chinese and western medicine treatment group was 98.33%, higher than the western medicine treatment group (P<0.05);the levels of SDNN,SDANN,RMSSD and PNN50 (%) were significantly higher in the the traditional Chinese and western medicine treatment group than those in the western medicine group (P<0.05);the levels of SV,LVPS and LVEF in the traditional Chinese and western medicine treatment group were higher than that in the western medicine treatment group ( P <0.05 );the sleep quality of the traditional Chinese and western medicine treatment group was significantly improved compared with the western medicine treatment group(P<0.05);the abdominal distension, nausea and vomiting,fatigue and cough incidence were no difference between the two groups.Conclusion Danhong injection combined with metoprolol tartrate tablets arrhythmia in menopausal women has a better therapeutic effect ,can obviously improve the patients with heart rate variability and cardiac function and adverse reactions occurred rate is low.

Objective To investigate the effects of sevoflurane anesthesia in diabetic pregnant rats on the cognitive function of the offspring rats. Methods Forty female Sprague?Dawley rats and 5 male rats, weighing 200-250 g, were used in the study. Twenty pregnant rats at 7 weeks of gestation were randomly selected, and diabetes mellitus was induced by intraperitoneal streptozotocin 45 mg∕kg and confirmed by blood glucose level>10.4 mmol∕L. Twenty pregnant rats at 20 days of gestation, in which diabetes mellitus was not induced, were selected and divided into 2 groups ( n=10 each) using a random number table:sevoflurane group (group S) and control group (group C). Twenty pregnant rats at 20 days of gestation with diabetes mellitus were selected and divided into 2 groups ( n=10 each) using a random number table:sevoflurane group (group DS) and control group ( group DC). In DS and S groups, the pregnant rats were placed in a self?made anesthetic box and inhaled 2% sevoflurane for 2 h. At 6 weeks after birth, the offspring rats were selected, and Morris water maze test was performed. The rats were sacrificed, brains were removed, and the hippocampi and cortex were removed for determination of phosphorylated cyclic a?denosine monophosphate response element?binding protein ( p?CREB) expression using immuno?histochem?istry. Results Compared with group C, the escape latency was significantly prolonged, and the frequency of crossing the original platform was significantly decreased in S and DC groups ( P<0.05) . Compared with group DC, the escape latency was significantly prolonged, and the frequency of crossing the original plat?form was significantly decreased in group DS (P<0.01). Compared with group S, the escape latency was significantly prolonged, the frequency of crossing the original platform was significantly decreased ( P<0.05) , and lighter staining for p?CREB was found, and the number of p?CREB positive cells was decreased in the hippocampus and cortex in group DS. Conclusion Sevoflurane anesthesia?induced cognitive dys?function is aggravated in the offspring rats of diabetic pregnant rats, and the mechanism is related to inhibi?tion of CREB phosphorylation.