1. The nutritive and biological values of the dry heat-treated beef protein were estimated by the growth and the nitrogen balance methods, using weaning and young rats respectively.2. At a 10 per.cent level of protein intake in the diet, the digestibility and biological values of the beef protein dried at two different temperatures were respectively as follows: at 60℃, 94.1 and 85.5; at 140℃, 88.3 and 76.1.3. The protein efficiency of beef dried at 60℃, 80℃, 100℃ and 140℃ obtained at a 10 per cent level of protein intake during a period of 4 weeks were as follows: 60℃, 2.70 + 0.09 g; 80℃, 1.79?0.12g; 100℃, 1.78?0.13 g; and 140℃, 1.33?0.35g.4. The higher the temperature, the lower the nutritive and biological values of dried beef protein. This decrease appears to be the result of lowered digestibility of the dried beef protein due to a change in the peptide linkages which renders them less susceptible to enzymatic hydrolysis.

Background and purpose:Epirubicin is one of the fi rst line chemotherapy drugs in the treatment of breast cancer, and liposome doxorubicin is a new antitumor drug that has been reported to have less cardiotoxicity and myelosuppression compared to free doxorubicin. Dentritic cells (DC) play important roles in tumor immunity. Our experiment investigated the impacts of epirubicin and liposome doxorubicin on different human breast cancer cell lines and dentritic cells, and evaluated their roles in the treatment of breast cancer. Methods:Human breast cancer cell lines, Bcap37 and MDA-MB-231, along with human dentritic cells isolated and induced into maturation, were cultured with epirubicin and liposome adriamycin at different concentrations (0, 0.25, 0.5, 1.0, 2.0, 4.0, 10.0 ?g/ml), respectively. The inhibitory effects were detected by MTT method after 24, 48, 72 h. Results:Epirubicin and liposome adriamycin could inhibit the proliferation of Bcap37 cells, MDA-MB-231 cells, and human dentritic cells. Liposome adriamycin exhibited a lighter inhibition on dentritic cells than on human breast cancer cell lines (Bcap37 and MDA- MB-231) (F=22.208, P

Objective To explore the clinical significance of detection of IL-17 and peripheral blood eosinophil(EOS) count in whee-zing children under 5 years old.Methods Fifty-three children with recurrent wheezing under 5 years old had been randomly enrolled including 43 boys and 10 girls.They were divided into 2 groups :wheezing group Ⅰ,in which,the patients with atopic high risks(n=27);whee-zing group Ⅱ,in which,the patients without atopic high risks(n=26).Children without infectious diseases such as hernia and renal calculus had been enrolled randomly as control group(n=20),including 11 boys and 9 girls.After taking 3 mL venous blood and centrifuging of patients in 3 groups,the level of IL-17 in the serum was measured by double-antibody sandwich enzyme-linked immunosorbent assay,peripheral blood EOS count was performed in the meantime by means of eosine staining.Results The serum levels of IL-17 in wheezing groups [(1 469.315?978.300) ng?L-1,(263.340?131.800) ng?L-1]were significantly higher than that of control group[(36.478?2.000) ng?L-1](Pa0.05);serum IL-17 and peripheral blood EOS count had positive correlation(r=0.933,P

Isolated pancreatic islet cells from neonatal rats were cultured in vitro. Insulin release was significantly inhibited by interleukin-1?. Addition of testosterone to the IL-, 1?-impaired pancreatic islets could increase insulin release, suggesting a protective effect of testosterone on pancreatic islet cells. Testosterone also significantly increased the insulin content in islet cells, and this effect could be blocked by actinomycin-D, indicating that testosterone promoted the synthesis of DNA and insulin may be one of its major protective mechanism.

Objective To evaluate the value of CT and MRIin diagnosis of littoral cell angioma(LCA) of spleen.Methods CT and MRI findings the data of 6 patients with LCA were rospectively analyzed,and all patients were proved LCA of spleen by pathology.Then enhanced CT (n =4 cases) and MRI (n =2 cases) were performed.The imaging performance of LCA was observed,and its imaging features was summarized.Results None of patients showed obvious signs and symptoms of hypersplenism,4 cases were with splenomegaly.5 patients had a single lesion,only one had few lesions.There were not any calcification or envelopement of all the lesions.On CT,all lesions demonstrated distinct borders.The enhanced scan for low-density nodules demonstrated slow progressive enhancement.On MRI,all the LAC had well circumscribed borders,and demonstrated T1 · hypointense and T2-equal,hyperintense signals,and progressive enhancement on the post contrast images.Conclusion CT and MRI imaging of littoral cell angioma of spleen have special imaging characteristics,those particular findings is helpful in the diagnosis.

OBJECTIVETo study the types of matrix metalloproteinases (MMPs) involved in dentin bonding interface degradation.

METHODSDentin slices were prepared and treated with two adhesive systems (Single Bond 2 or Clearfil S3 Bond). The dentin surface was bonded with composite resin. All specimens were immersed in sterile artificial saliva for 0 or 6 months, and their micro-shear bond strength (muSBS) were measured. The fracture modes were observed through field emission scanning electron microscope (FE-SEM). Dentin slices with 4 mm x 3 mm x 1 mm dimensions were prepared. The slices were divided into three groups according to the treatment modes (negative control, Single Bond 2, and Clearfil S3 Bond). All specimens were stored in sterile artificial saliva for 0 or 6 months. The concentrations of MMP-1, -2, -3, -8, and -9 of each group were detected through fluorescent microsphere immunoassay.

RESULTSThe muSBS of both adhesive systems significantly decreased after storage aging. Significant differences in failure modes within the four groups tested in this study were observed. Compared with the negative control, the concentrations of MMP-1 and MMP-3 in different adhesive groups showed no significant difference after storage aging. However, the concentrations of MMP-2, -8, and -9 in Single Bond 2 group and the concentrations of MMP-8 and -9 in Clearfil S3 Bond group significantly decreased after 6 months of storage aging.

CONCLUSIONSignificant degradation occur in the dentin bonding interface of both adhesive groups under 6 months aging challenge. The concentrations ofdentinal MMP-2, -8, and -9 significantly decrease after treatment with adhesives and aging, indicating that these MMPs have an important function in dentin bonding interface degradation.

Aged ; Female ; Hearing Loss, Bilateral ; diagnosis ; etiology ; Humans ; Osteitis Deformans ; complications ; diagnosis

Objective To discuss manifestations of neurogenic tumors on chest wall on CT imaging and pathology.Methods 7 patients with neurogenic tumors on chest wall confirmed surgically and pathologically were reviewed.To facilitate differential diagnosis,3 cases of malignant sarcoma were reviewed for their features on CT.Results 4 out of 5 cases of neurinoma were benign,with one case showing even density on plain CT scan,3 were poorly even,one case showed obvious evenness after enhancement,and 1 case was poorly evenly enhanced,2 benign cases had compression absorption of surrounding bone of scapula or ribs.One case of moderate malignancy was uneven in plain scan and slightly enhanced after enhancement procedure,and neighboring bone of scapula and rib were compressed and destroyed and absorbed,and neighboring muscular interspace and upper mediastinum were involved.One case showed single lesion of neurofibroma,displaying relatively even density on plain scan and moderate unevenness after enhancement procedure,as well as compression and absorption of neighboring ribs.One case of neurofibroma had multiple lesions,showing uneven density on plain scan.This case did not undergo enhanced scan.The remaining 3 cases were all sarcoma including 2 cases of fibrosarcoma and one case of synovial sarcoma.Conclusion Mass of soft tissues that are situated on chest wall or paravertebral area and with smooth edge are suggestive for benign neurogenic tumor.On the contrary,rough edge and coarse shape and infiltration into neighboring tissues are characteristic for malignant ones.

Objective To evaluate the etiologies of children with gastrointestinal bleeding(GIB), and the relationship between the pathological findings and clinical.Methods Gastroscopy or proctoscope was performed in 153 children with GIB. Pathological studies and tests as for helicobactor pylori (Hp) were undertaken.Results Among 153 children,140 cases(91.5 %) had definite diagnosis,including 74 children with upper gastrointestinal bleeding(UGIB) and 66 cases with lower gastrointestinal bleeding(LGIB). Gastric pathologic study was conducted in 56 cases. All patients had chronic superficial gastritis(CSFG). Hp test was positive in 33 patients. There was significant difference in the positive rate of Hp test between patients with gastritis or duodenitis and those with ulcers (P

Objective To prepare 2-[18F]fiuoropropionic acid (18F-FPA) and evaluate its biodistribution and imaging capacity in Lewis lung carcinoma-bearing mice.Methods 18F-FPA was prepared by nucleophilic substitution reaction,and its hydrophilicity was analyzed.18F-FPA (7.4-11.1 MBq) was injected into Lewis lung carcinoma-bearing mice via tail vein.MicroPET imaging was performed at 20,80 min after the injection.The biodistribution of 18F-FPA in organs was analyzed.The blocking effects of sodium propionate and dichloroacetate to 18F-FPA were tested in vivo.Data were analyzed by two-sample t test using GraphPad Prism software.Results The synthesis of 18F-FPA took 40 min.18F-FPA had high radiochemical purity (＞99％) and hydrophilicity.18F-FPA was mainly distributed in the carcinoma,the urinary bladder and the caecum.The radioactive uptakes in muscles,brown fat and bones were relatively low.Quantitative analysis showed that the uptake of 18F-FPA in Lewis lung carcinoma from 20 min to 80 min was slightly increased ((17.03±2.87) ％ID/g vs (19.33±2.45) ％ID/g) without significant difference (t=1.100,P＞0.05).Neither sodium propionate nor dichloroacetate could block the uptake of 18F-FPA in Lewis lung carcinoma (t=1.544,0.894;both P＞0.05).Conclusions 18F-FPA can be quickly synthesized and has good physicochemical properties.It can be used as a tracer to visualize Lewis lung carcinoma in mice,and its tumor uptaking can not be blocked by propionate and dichloacetate.18 F-FPA PET has the potential to detect lung cancer noninvasively in clinic.