Objective: To establish a quantitative method for determination of protocatechuic acid in Xueniao Capsules. Methods: The capsules were extracted with alcohol acetic acid (10∶1) and then solved with absolute alcohol. The sample was applied on a silica H plate, developed and separated in the solution of chloroform acetone methanol acetic acid (7∶2∶1.5∶0.5). The separated spots were determined by TLC scanning at 530nm(? s). Results: The average recovery was 101.4%. Conclusion: This method for determination of protocatechuic acid in Xueniao Capsules was sensitive, accurate and specific. It could be used as quality control for Xueniao Capsules.

For 10 years recently,China has implemented the strategy of "rejuvenating technology,talents for the first",and China's higher education has made improvement significantly: the number of student in-class increases drastically,the gross enrollment rate of higher education reached the level of popular education.Kunming Medical College,in this particular historical period of development by accelerating the construction and structural adjustment, strengthening management,taping potential,integrating use of resources,has better dealt with the enormous pressure and the challenge caused by the school's operating scale's nearly five times increase,and has ensured the quality of student training;In the new historical period of development, Kunming Medical College will continuously change education ideas,achieve the leap development from teaching university to teaching and research university positively.

The hypo-morality,also known as sub-morality,is an imperfect morality compared with the social mainstream morality.By introducing some "hypo-morality" phenomena in the current physician-patient relationship and analyzing from the two aspects including the diversity of interests main bodies and the market operational mode of healthcare institutions,this paper assures the rationality of the existence of hypo-morality in physician-patient relationship,and attempts to provide feasible suggestions for a reasonable direction of hypo-morality in physician-patient relationship.

Objective:To investigate the clinical efficacy ofacupuncture plus Tuina therapy in treating supraspinatus tendinitis.Methods:One hundred patients with supraspinatus tendinitis were randomly allocated to two groups.The treatment group of 50 cases was treated with acupuncture plus Tuina therapy and the control group of 50 cases,with simple acupuncture.Treatment was given once daily,10 times as a course.The therapeutic effects were evaluated after two courses oftreatment.Results:The total efficacy rate was 96.0%in the treatment group and 74.0%in the control group.x2 test showed that there was a statistically significant difference in the total efficacy rate between the two groups(P＜0.01).Conclusion:Acupuncture plus Tuina therapy is superior to simple acupuncture in treating supraspinatus tendinitis.

The contrast of technical force between hospital and society is astonishing in the maintenance & service of large-scale medical equipment.When the maintenance & service plan must be chosen to socialized service,all the plans must be seriously compared and evaluated according to the current situation of the equipments in hospital for an economical and suitable selection.A right selection is necessary in promoting maintenance and management of large-scale medical equipment,keeping good operation of equipment,prolonging service life,and creating better economic and social benefits.

Objective To explore the correlation between serum HBV-DNA content and hepatic fibrosis indicators in hchronic epatitis B patients.Methods Serum HBV-DNA content was determined by using real-time fluorescence quantitative PCR and he-patic fibrosis indicators including hyaluronic acid,laminin,procollagen Ⅲ,and collagen Ⅳ which were determined by using chemilu-minescence.Results The logarithm value of serum HBV-DNA content of the 110 hepatitis B patients was 5.32±1.37.Serum hyal-uronic acid concentration was (197.81 ±85.37)mg/mL;procollagen Ⅲ concentration was (142.66 ±30.28)μg/mL.Collagen Ⅳconcentration was (90.34 ±20.53)μg/mL.Laminin was (143.96 ±31.79 )μg/mL.All these indicators were significantly higher than the control group (P <0.01).But there were no significant linear correlation between serum HBV-DNA content and the four hepatic fibrosis indicators(P >0.05).Conclusion The hepatic fibrosis indicators increased significantly in hepatitis B patients but there were no significant correlations between serum HBV-DNA content and the hepatic fibrosis indicators.

Objective To investigate the relationships between fasting plasma glucose (FPG) level in early pregnancy and gestational diabetes mellitus (GDM).Methods Data of 11 477 pregnant women who accepted prenatal care in Beijing Obstetrics and Gynecology Hospital from October 2011 to September 2012 were collected.FPG was tested during 8 to 12 weeks of pregnancy in all women and those with FPG＜7.00 mmol/L were recruited.Women accepted 75 g oral glucose tolerance test (OGTT) during 24 to 28 weeks of pregnancy.The GDM diagnostic criteria was with reference to the criteria of International Association of Diabetes and Pregnancy Study Group.Mann-Whitney U test was used to analyze the difference of early pregnancy FPG between normal pregnant women and GDM women.Receiver operating characteristic (ROC) curve was used to analyze the validity and applicability of using early pregnancy FPG in GDM diagnosis.Chi-square test was used to analyze the relationship between the FPG levels and GDM diagnosis.Results There were 1 535 (13.4％) women diagnosed as GDM in 24 to 28 weeks of pregnancy (the rest 9 942 normal cases were taken as the controls).The median FPG level of the GDM group was 4.89 mmol/L (4.62-5.15 mmol/L),which was higher than that of the controls [4.75 mmol/L(4.53-4.98 mmol/L)] (Z=-13.994,P=0.000).The maximum area under curve (AUC),which was used to predict GDM with early pregnancy FPG,was 0.599 (95％ CI:0.582-0.617).Taking FPG 4.88 mmol/L as the cutoff value,the sensitivity was 0.523 and the specificity was 0.645.While taking FPG 5.10 and 5.60 mmol/L as the cutoff value,the sensitivity was 0.334 and 0.068,and the specificity was 0.811 and 0.983,respectively.When the FPG level ≤ 4.09,-4.60,-5.10,-5.60,-6.10 and ≥ 6.10 mmol/L,the GDM diagnostic rate gradually increased [8.5％(23/212),9.9％(335/3 379),12.3％(719/5 858),20.7％(359/1 734),40.2％ (78/194) and 52.5％ (21/40)] (x2=300.523,P=0.000).GDM diagnostic rate in FPG ≤ 4.09,-4.60,-5.10 and-5.60 mmol/L group were lower than that in FPG ≥ 5.60 but ＜6.10 mmol/L group and ≥ 6.10 mmol/L group (x2 were 67.242,164.680,128.125,37.860,55.843,76.856,58.589 and 23.484,all P=0.000) ; GDM diagnostic rate in FPG ≤ 4.09,-4.60,-5.10 mmol/L group were lower than that in FPG ≥ 5.10 but ＜5.60 mmol/L group (x2 were 22.877,113.717 and 78.040,all P=0.000); GDM diagnostic rate in FPG ≥ 4.09 but ＜4.60 mmol/L group was lower than that in FPG ≥ 4.60 but ＜5.10 mmol/L group (x2=11.803,P=0.001).When using abnormal fasting and postprandial OGTT level as GDM diagnostic criteria,the ratio of GDM in early pregnancy FPG level ≥ 5.60 but ＜6.10 mmol/L group and FPG ≥ 6.10 mmol/L group were higher than that of the FPG level ＜5.60 mmol/L group [50.0％ (39/78) and 71.4％ (15/21) vs24.1％ (346/1 436),x2 were 12.456 and 21.443,all P＜0.003].Conclusions Early pregnancy FPG level is not proper to be used as an early diagnostic tool of GDM.However,when early pregnancy FPG level is equal to or greater than 5.60 mmol/L,the incidence of GDM in late pregnancy will increase significantly.

Objective To evaluate the value of serum tumor markers in diagnosis of over 80 years old patients with colorectal cancer.Methods Serum CA199,CA242 and CEA of 68 cases senile patients with colorectal cancer and 35 cases senile patients with benign large intestinal diseases were detected by ELISA.Results Serum levels of CA199,CA242 and CEA in patients with colorectal cancer were (37.95 ± 15.95 ) U/ml,( 50.08 ± 24.20 ) U/ml,( 22.34 ± 17.35 ) μg/L respectively and were significantly higher than that in patients with benign large intestinal diseases CA199 ( 10.73 ± 6.27 ) U/ml,CA242 ( 8.00 ± 3.52 ) U/ml,CEA(2.32 ± 1.01 )μg/L.In addition,the level of these three markers was increased with the progress of colorectal cancer and the sensitivity of diagnosis was related with the stages of colorectal cancer.Parallel combined testing methods increased the diagnostic sensitivity to 83.6％,and serial combined testing increased the diagnostic specificity to 88.7 ％.Conclusions Serum tumor markers showed certain values in the diagnosis of the senile patients with colorectal carcinoma.Furthermore,the combined determination of tumor markers is helpful for early diagnosis of colorectal cancer and increases the sensitivity and specificity in diagnosing of colorectal carcinoma.

Objective To investigate the effect of Naloxone in the pre-hospital care of AOPP.Methods 54 patients with the pre-hospital care of AOPP were divideded into two groups.The treatment group was treated with Naloxone,Atropine and Pyridine aldoxime methyliodide,the control group treated with Atropine and Pyridine aldoxime methyliodide,and the results were analyzed using t-test and x2 test.The efficacy of the two groupswas compareded.Results In the treatment group,the mumber of drug delivery and the dosage of Atropine were less.The recovery time of the actirity of Cholinesterase and the patients coma were shorter.Complications and mortality was significantly lower,but the cure rate was significantly higher than that of the control group.Conclusion Naloxone is effective and safe in the treatment the pre-hospital care of AOPP.It is worthy of clinical promotion.

Objective: To investigate the clinical effect of mind-regulating acupuncture therapy on peripheral facial paralysis. Methods: Two hundred subjects were divided into observation group (treated by mind-regulating acupuncture therapy) and control group (treated by points of Yang-ming meridians) with one hundred in each group. The points of Yang-ming meridians were mainly selected for control group, observation group were treated by points with the function of regulating mind besides the points of control group, for two courses with 10 treatments in course. Results: The cure rate was 65.0% and total effective rate was 99.0% in observation group, while in control group, the cure rate was 55.0% and total effective rate was 91.0%, the curative effect in observation group was prior to control group (P＜0.05). Conclusion:The therapy of mind-regulating acupuncture gives a quicker response, a shorter course of treatment and a higher cure rate.