OBJECTIVE: To develop a method for determination of the content of Dexamethasone acetate in Compound tretinoin ointment by RP-HPLC. METHODS: The separation was performed on C18 column with methanol: water (70∶30) as a mobile phase, wavelength of UV detector at 240nm. RESULTS: The linear range of Dexamethasone acetate was within 0.028 3～0.849 0?g(r=0.999 9). The average recovery of Dexamethasone acetate was 101.13%, with RSD at 1.28%. CONCLUSION: The method is simple, rapid and capable of producing good separation results. It can be applied to the quality control of Compound tretinoin ointment.

OBJECTIVE:To investigate situation and trend of proton pump inhibitors in 19 hospital of Hangzhou area and to provide reference for the research,production,sales and clinical application of this kind of drugs. METHODS:Utilization of proton pump inhibitors in 19 hospital of Hangzhou area during the period of 2004～2008 were analyzed statistically in respect of consumption sum,DDDs and DDC. RESULTS:Both consumption sum and DDDs of proton pump inhibitors increased quickly in recent five years. Amount of oral omeprazole(capsule and tablet)was the greatest among those categories and increased steadily. Amount of rabeprazole and esomeprazole as new type of proton pump inhibitors increased rapidly. CONCLUSION:Proton pump inhibitors have great potential for development. Omeprazole are widely used in the clinic as mature proton pump inhibitors.

Echocardiography, Three-Dimensional ; Heart Defects, Congenital ; diagnostic imaging ; therapy ; Humans

Objective To investigate and improve the methods of nasal feeding to reduce the occurrence of complication such as diarrhea and vomiting.Methods We separated 140 patients with heavy craniocerebral injury into the control group (50 patients) and the experimental group (90 patients) according to different nasal feeding methods. For the control group traditional method was used. For the experimental group infusion pump was used to control infusion rate. The incidence of complication was compared between two groups. And within experimental group, the same nutrient solutions in respect of type and volume were infused during 10 hours, 16 hours, and 18 hours respectively. The relationship between infusion rate and the occurrence of complication was observed, analyzed and compared to seek for the most suitable enteral nutrition route which incurred the least complication.Results The incidence of diarrhea and vomiting in the experimental group was lower than that of the control group. And there were no significant differences in the occurrence of complication by using infusion pump at three different infusion rates within the experimental group. Conclusions The application of infusion pump in nasal feeding can reduce the incidence of complication and infusing nutrient solution at a slow rate was not necessary.

Endocrine System Diseases ; diagnosis ; Hearing Disorders ; diagnosis ; Humans ; Infant, Newborn ; Metabolism, Inborn Errors ; diagnosis ; Neonatal Screening ; methods ; standards

0.05)but a statistical difference between groups I and groups III(P

BACKGROUND:Titanium zirconium niobium tin al oy (Ti-Zr-NB-Sn al oy) has good tissue structure, mechanical properties, corrosion resistance and biocompatibility, which is an ideal dental material. OBJECTIVE:To explore the efficacy and safety of Ti-Zr-NB-Sn al oy in dental restoration. METHODS:A retrospective analysis was performed on clinical data of 87 cases of dental defects, including 43 males and 44 females, aged 22-41 years. Of the 87 cases, 43 cases were repaired with Ti-Zr-NB-Sn al oy as observation group, and the other 44 cases were treated with nichrome as control group. After 36 months of fol ow-up, the therapeutic efficacy, dental restoration conditions, dental prosthesis conditions and patient satisfaction were observed and compared. RESULTS AND CONCLUSION:Al the 87 patients were fol owed up for 36 months. After the fol ow-up, the total effective rate and patient satisfaction were higher in the observation group than the control group (P<0.05). In the control group, there were four cases of swol en gums, four cases of bleeding gums, four cases of al ergies, three cases of gum gray line, and six cases of mucosal pain;in the observation group, there was only one case of swol en gums, and the dental restoration was better in the observation group than the control group (P<0.05). For the dental prosthesis, there were 10 cases of dental stent damage and 20 cases of dental stent loosening in the control group;and only one case of dental stent damage and one case of dental stent loosening in the observation, and the observation group was superior to the control group in the dental prosthesis (P<0.05). Experimental findings suggest that Ti-Zr-NB-Sn al oy for repair of dental defects has good biocompatibility and security.

Objective To observe the connections between enkephalin immunoreactive(ENK-ir) terminals and ?-aminobutyric acid immunoreactive(GABA-ir) neurons in the rostral portion of the nucleus tractus solitarious(rNTS) of the rat. Methods Double-immunofluorescent labeling method and the pre-embedding immunohistochemical staining combined with immuno-gold particles labeling technique for electron microscopic detection were used in the present study. Results Under the laser scanning confocal microscope,dense ENK-ir fibers and terminals and some GABA-ir neurons were observed in the rNTS.Close contacts between ENK-ir terminals and GABA-ir cell bodies were also detected.By using the electron microscopic technique,ENK-ir reaction products were found to be mainly localized on the surface of round clear vesicles and dense-cored vesicles in the axon terminals.ENK-ir terminals formed symmetric,which was a dominant pattern,and asymmetric synaptic connections with GABA-ir and immunonegative cell bodies and dendrites.Conclusion The ENK-ir terminals might take part in the transmission and regulation of the taste information in the rNTS through inhibiting,exciting the GABAergic neurons or inhibiting directly the activity of neurons within the rNTS.