Objective To probe into clinical application value of tetramethylpyrazine injection as an adjuvant of 1,6-diphosphate in the treatment of neonatal hypoxic-ischemic encephalopathy.Methods From January 2012 to December 2012,98 neonatal hypoxic-ischemic encephalopathy children patients were selected in the Second Affiliated Hospital of Shenyang Medical College.The children patients were divided into observation group and control group randomly.There were 49 cases in each group.Both two groups adopted 1 ,6 diphosphate - fructose,and the observation group adopted ligustrazine injection on the basis of control group.The clinical treatment and prognosis of the children patients in two groups were compared and analyzed.Results The rates of significant efficiency and total efficiency were 67.35%and 93.88%in observation group,which were higher than those in control group (51.02%,71.43%),and there were statistically significant (P <0.05 );compared with control group,the NBNA scores of observation group were improved significantly after 3rd,7th and 14th days treatment,and there were statistically significant (P<0.05);compared with control group,the ratio of cerebral palsy,epilepsy,mental retardation and death in observation group showed different degree of reduction,but there were no statistically significant.Conclusion The effect of tetramethylpyrazine injection adjuvant 1 ,6-diphosphate in treatment of neonatal hypoxic-ischemic encephalopathy was effective and significant.It has positive role in promoting the improvement and upgrading of the clinical efficacy and prognosis.

A large number of animal experiments have confirmed that ischemic preconditioning can produce a powerful organ protective effect,but the progress and results of the conversion of animal experiments to clinical trials are unsatisfactory.It has great significance for studying of molecular mechanisms of ischemic preconditioning mediated neuroprotection,searching for safe and effective preconditioning induced ways which can be converted to clinical practice,improving the tolerance of nerve tissue ischemia and hypoxia in stroke and surgical patients and achieving a safe and effective neuroprotection.This article reviews the molecular mechanisms of ischemic preconditioning mediated neuroprotection from the aspects of pretreatment of activated receptor,mitochondria,transcription factor,and protein kinase.

OBJECTIVE To search the methods of decreasing the missing report of nosocomial infection cases with a prospective monitoring method.METHODS To summarize the methods and measures that had been adopted for ten years.RESULTS There were four important means to decrease the missing report of nosocomial infection cases with a prospective monitoring method.The measures included combining prospective monitoring method and(retrospective) monitoring method at the beginning of taking prospective monitoring method,taking the right(methods),devising careful and precise researching procedure,reinforcing to propagandize and educate the(knowledge) of nosocomial infection to enhance the understanding of medical personnel to control nosocomial(infection),and analyzing and feedbacking the data of nosocomial infection with a prospective monitoring method.CONCLUSIONS It is necessary to develop the prospective monitoring method of investigating nosocomial(infection) as perfectly as possible.It can not only decrease the missing report of nosocomial infection with(prospective)(monitoring) method,but also effectively clue on trend of outbreak and epidemic of nosocomial infection.

Objective To explore the difference of the curative effect between incision-thread-drawing procedure and traditional incision drainage in the treatment of perianal abscess.Methods A total of 150 patients diagnosed with perianal abscess and treated at the first time from June 2015 to June 2016 in our department were selected as the research object in this experiment,who were divided into the observation group(75 cases) and control group(75 cases) according to the surgical method.Patients in the observation group were performed with incision-thread-drawing procedure,while the control group were treated by traditional incision drainage.The overall treatment effect,operation time,postoperative recovery time,hospital stay,hospital costs,wound healing time and postoperative complications between the two groups were observed and compared.Results The difference of the overall treatment effects between two groups were not statistically significant(P>0.05).The operation time,the postoperative recovery time,the hospitalization time and the cost,the time of wound healing of observation group were significantly better than the ones of the control group,the differences were statistically significant(P<0.05).The recurrence rates of anal fistula,abscess and other complications in patients of observation group were significantly lower than the ones of control group.The differences were statistically significant(P<0.05).Conclusion Compared with traditional incision drainage,the incision-thread-drawing procedure is more effective with less complications and lower recurrence rate,which should be the first choice for the treatment of perianal abscess.

Objective To observe the clinical efficacy of procedure for prolapse and hemorrhoids ( PPH) for treatment of rectal mucosa prolapse.Methods The 100 patients who suffered from rectal mucosa prolapse and were planning to take surgical treatment for the first time in our hospital from June 2013 to June 2015 were randomly divided into the treatment group and the control group .Patients in the treatment group(n=50) underwent PPH while patients in the control group (n=50) underwent traditional simplified mucosal plication technique .To observe the therapeutic effect of the two groups ,the average operation time ,average length of stay in hospital ,postoperative pain within 72 hours,postoperative anal function and postoperative complications of the two groups .Results There was no significant difference in the over-all treatment effect between the two groups(P>0.05).The average operation time, average length of stay in hospital,postoperative pain with-in 72 hours and postoperative anal function of the treatment group were lower than those of the control group ,and the differences were significant (P<0.05).Bleeding and anal bearing-down occurred much less in the treatment group compared with the control group ,and the differences were statistically significant(P<0.05).But there was no significant difference in postoperative complications such as recurrence ,rectal steno-sis and anal incontinence (P>0.05).Conclusion Compare to the traditional simplified mucosal plication technique ,PPH technique has more advantages such as shorter operation time ,less anguish of patients,less complication,shorter hospitalization time,and higher security, which indicates PPH could become the preferred method for treatment of rectal mucosa prolapse .

Objective To evaluate superselective external carotid artery embolization before transnasal endoscopic resection in treating nasopharyngeal angiofibroma.Methods Superselective external carotid artery embolization was performed in 20 patients with nasopharyngeal angiofibroma one to three days before transnasal endoscopic resection was carried out.The clinical data,including the volume of blood loss during the surgery,the operative time and the complications,were retrospectively analyzed.Results Superselective external carotid artery embolization was successfully preformed in all 20 patients,which was followed by the transnasal endoscopic resection within 3 days.The average volume of blood loss during the surgery was 155 ml and the total time of operation was 75 minutes.Mild complications occurred in 6 patients after embolization,which were subsided after symptomatic management.No serious complications occurred after surgical resection.The mean hospitalization days of the patients were 3.5 days.Conclusion Preoperative superselective external carotid artery embolization of nasopharyngeal angiofibroma can markedly reduce the blood loss during surgical resection,apparently shorten the operative time and effectively lower the occurrence of complication after the operation.Superselective external carotid artery embolization combined with transnasal endoscopic resection should be regarded as an ideal therapy for nasopharyngeal angiofibroma.

The safety and effectiveness of catheter ablation in coronary sinus using radiofre-quency energy was studied in 16 dogs. Radiofrequency energy(1229?711 J) was applied between electrode catheter placed inside coronary sinus and a chest wall patch. Thirteen dogs were killed approximately half an hour after ablation and three 3 to 4 weeks. Lesion of abltion area were 3. 2?1. 8mm in length, 2. 2?1. 7mm in width and 2. 1?1. 0mm in depth. Microscopic examination showed well circumscribed area of coagultion necrosis (acute period)and fibrosis (chronic period). The coronary atery and mitral apparatus were not involved,nor was the endocadium. No arrhyth-mias except occationally atrial or ventricular premature beats were observed. No hemodynamic changes and rupture of coronary sinus occurred in any dog. Large amount of radiofrequency energy can be safely applied to the coronary sinus. The location of the lesions produced suggests that this technique may be useful for interruption of left-sided atioventricular pathways of patients.

Objective:To investigate the effects of E.coli lipopolysaccharide(LPS) stimulus on the synthesis of TLR4 protein and its mRNA expression in human dental pulp cells(HDPCs), and to explore the roles of TLR4 on the activation of HDPCs induced by LPS. Methods:The expressions of TLR4 mRNA and the synthesis of TLR4 protein in HDPCs induced by LPS were detected by real-time fluorescence quantitative RT-PCR(FQ RT-PCR) and immunofluorescence technique.IL-1? concentrations in the supernatant of cultured HDPCs pretreated with TLR4 antibody were assayed with ELISA. Results: The expressions of TLR4 could not be detected in normal HDPCs.After being stimulated with 1?10-4 g/L LPS for 6,12 or 24 h, immunostaining showed that TLR4 was expressed in cytomembrane and cytoplasm of HDPCs,while in nucleus the expressions were negative. FQ RT-PCR showed their expressions significantly increased after being stimulated with LPS (P

Objective To investigate the role of peripheral blood lymphocyte apoptosis through Fas/ Fas ligand pathway in the development of viral myocarditis. Methods Twenty-five viral myocarditis (VMC) and 25 healthy children were chosen for study. The apoptosis percentage in peripheral blood iymphocytes was quantitatively examined, flow cytometry with Annexin V/PI double parameter was used. And the expressions of Fas and Fasl protein in peripheral blood lymphocytes were analyzed with flow cytometry. Results The percentage of apoptosis in peripheral blood lymphocytes in children with VMC [(0. 6976 ± 0. 1109)%] was significantly higher than that of healthy children [(0. 1288 ± 0. 1069) %] (t = 18.459, P ＜ 0.001). Moreover, the apoptosis cell percentage showed a significant positive correlation with the serum levels of myocardium enzyme (creatine kinase-MB, lactate dehydrogenase, creatine kinase, hydroxybutyrate dehydrogenase)(r =0. 817 ,P ＜0. 01 ;r =0. 785 ,P ＜0.01 ;r =0. 726 ,P ＜0. 01 ;r =0. 712 ,P ＜0. 01). The expression of Fas and Fasl protein in VMC patients(2. 8804 ± 0. 3020,4. 4496 ± 0. 4836) were higher than those of health children(2. 1300 ±0. 6200,0. 1238 ±0. 0384) ,which had significant difference(t = 17. 321 ,P ＜0. 001 ;t' =44. 577, P ＜ 0. 001). The apoptosis percentage of peripheral blood lymphocytes in children with VMC showed a significant positive correlation with the Fas and Fasl protein expression in peripheral blood lymphocytes(r =0. 972, P ＜ 0. 01; r = 0. 958, P ＜ 0. 01). Conclusion Apoptosis percentage of peripheral blood lymphocytes increases among children with VMC, probably because immune response decreased or it is related with the degree of myocardium damaged. Fas and Fasl play an important role in apoptosis of peripheral blood lymphocytes in children with VMC.

Tegument protein VP22 is encoded by Pseudorabies Virus (PRV) UL49. To identify the nuclear localization signals of UL49, it is necessary to determine the transport mechanism and biological functions of the VP22 protein. In this study, we identified two nuclear localization signals from UL49, NLS1 (5RKTRVA ADETASGARRR21) and NLS2 (241PGRKGKV247). The functional nuclear localization signal (NLS) of UL49 was identified by constructing truncated or site-specific UL49 mutants. The deletion of both NLS1 and NLS2 abrogated UL49 nuclear accumulation, whereas the deletion of NLS1 or NLS2 did not. Therefore, both NLS1 and NLS2 are critical for the nuclear localization of UL49. And our resuts showed that NLS2 is more important in this regard.
Animals ; COS Cells ; Cell Nucleus ; metabolism ; virology ; Cercopithecus aethiops ; Herpesvirus 1, Suid ; chemistry ; genetics ; metabolism ; Humans ; Nuclear Localization Signals ; Protein Transport ; Pseudorabies ; metabolism ; virology ; Viral Structural Proteins ; chemistry ; genetics ; metabolism