Objective To observe ocular manifestations of acquired immunodeficiency syndrome (AIDS). Methods Fourty-two AIDS patients (66 eyes)with ocular complaints received examinations of visual acuity, slit-lamp microscope, ophthalmoscope and fundus fluorescence angiography (FFA). The results were retrospectively analyzed. ResultsThere are five types of ocular findings, including cytomegalovirus (CMV) retinitis (37 eyes, 56.0%), retinal microvasculopathy of human immunodeficiency virus (21 eyes, 32.0% ), optic nerve diseases (three eyes, 4.5 % ), retinal neuroepithelial layer detachment (two eyes, 3.0%) and uveitis (three eyes, 4.5%). Conclusions The common ocular manifestations showed progressive necrotic retinitis, retinal hemorrhage and retinal vasculitis and attenuated, cotton-wool spots in AIDS patients.

Objective To research nitric oxide( NO) concentration, induced nitric oxide synthase( iNOS) mRNA expression and the pathological changes of lung tissue in traumatic shock rats of early stage preconditioned with heat stress. Methods A total of 98 male SD rats were equally divided randomly into heat stress untreated group and heat stress treatment group, and each group were equally divided into 7 subgroup: control group and shock 0 h group, 0. 5 h group, 1. 0 h group, 1. 5 h group, 2. 0 h group, 3 h group. To established the model of traumatic shock after heat stress pretreatment and remained lung tissue, observed the changes of pulmonary pathology, meas-ured the changes of the NO concentration and iNOS expression in lung tissues. Results The lesions of lung tissue structure were more lighter in heat stress treatment group than the corresponding time point of heat stress untreated group and lung histopathology score lower. The NO concentration of heat stress treatment group were lower than the corresponding time point of heat stress untreated group, the two groups have a rise at shock 0 h,the peak value of NO concentration in heat stress treatment group appeared later than the heat stress untreated group. INOS mRNA expression quantity of heat stress treatment group were lower than the corresponding time point of heat stress un-treated group, the peak value of heat stress untreated group in 2.0 h and heat stress treatment group in 1. 5 h, it was positively correlated with lung injury score. Conclusion Heat stress pretreatment can drop NO concentration and iNOS mRNA expression in lung tissues significantly, delayed and reduced the lung injury in traumatic shock rats of early stage.

Laparoscopic surgery is widely used in the treatment of gynecological malignancies,including the radical surgery or staging surgery of early cervical cancer,endometrial cancer and ovarian cancer.Its feasibility and safety of clinical practice in gynecologic malignancies has been approved,and the postoperative follow-up results indicate that the effectiveness of laparoscopic surgery is similar to open surgery.But the laparoscopic surgery treatment of gynecological malignancies is still in dispute.The research will focus on the affects of pneumoperitoneum to the malignant behavior of tumor cells and the mechanism about implantation metastasis of puncture site in future.

Apoptosis ; Humans ; Periodontitis ; pathology

Objective: To investigate the relationship of TNF-A-863 and CGRP979 gene polymorphisms with the susceptibility to severe chronic periodontitis in Chinese. Methods: Buccal swabs were collected from 100 adult patients with severe chronic periodontitis and 118 healthy adult controls. DNA was extracted from each subjects of the two groups. PCR-LDR technique was used to identify the genotypes of TNF-A-863 and CGRP979. The difference in the genotypes between the two groups was analyzed by statistics software. Results: The genotype of TNF-A--863 was mainly TNF-A-863 A/C in patients with severe chronic periodontitis and TNF-A-863 C/C in healthy controls. There were significant differences in TNF-A-863 distribution between the two groups( P<0.05). We also found that there were significant differences in genotype distribution of CGRP979 between the two groups (P<0.05) ,with A/C predominating in patients with severe chronic periodontitis. Condusion: TNF-A-863 polymorphism is associated with severe chronic periodontitis; A/C of the CGRP979 loci might be a factor for severe chronic periodontitis.

Adult ; Aged ; Cross Infection ; microbiology ; Drug Resistance, Bacterial ; Drug Resistance, Fungal ; Humans ; Male ; Middle Aged ; Pneumoconiosis ; complications

Objective To investigate the relationship between the polymorphism of the tumor necrosis factor-αgene-308 promoter and bronchial asthma (BA)in Uighur and Kazakh population in Xinjiang.Methods PCR product sequencing method was used to detect the polymorphism distribution of TNF-αgene in 60 BA patients and 60 controls among Xinjiang Uighur and Kazakh population.Then we analyzed the association between different TNF-αgenotypes and BA in Uighur and Kazakh population.Results The distribution of GG,GA and AA genotype was significantly different between the two groups.Statistical analysis showed that BA group had a significantly higher TNF-αA allele frequency than that of the control one (P <0.05).After adjustment for sex and age,we found the A allele was a risk factor for BA pathogenesis (P <0.05).Conclusion The polymorphism of TNF-α gene-308 may be associated with the susceptibility to BA in Xinjiang Uighur and Kazakh population,so TNF-α gene-308 may be considered as a genetic marker for early identification of individuals at high risk for BA,which may play an important role in preventing the development of BA in clinical practice.

Objective To observe the effect of acupuncture on the expressions of serum corticotropin-releasing hormone (CRH), pituitary adrenocorticotropic hormone (ACTH) and its mRNA, to discuss the predominant and secondary factors that influence the efficacy of acupuncture, and to explore the optimal combination of acupuncture parameters.Method The rat model of kidney yang deficiency was made by muscular injection with hydrocortisone. Serum CRH, pituitary ACTH and its mRNA expressions were taken as the observation indexes, and 4 influential factors (i.e. selection of acupoints, angle of needle twisting, frequency of needle twisting, and time of needle twisting) plus 3 levels of each factor were arranged to L9(34) orthogonal table. A computer-controlled acupuncture manipulation device was used to treat the kidney yang rats. Serum CRH and pituitary ACTH were detected by enzyme-linked immunosorbent assay (ELISA) and pituitary ACTH mRNA expression by real time fluorescent quantitative PCR, to illustrate the primary and secondary factors that affect the acupuncture effectiveness.Results Regarding the effect on acupuncture effectiveness, the time of needle twisting ranked the top, followed by frequency of needle twisting, angle of needle twisting, and selection of acupoints. Angle of needle twisting, frequency of needle twisting, and time of needle twisting should be the predominant factors or secondary factors in influencing acupuncture effectiveness (P<0.01 orP>0.05). Selection of acupoints should be an important factor or secondary factor in influencing acupuncture effectiveness (P<0.05 orP>0.05).Conclusion For rats with kidney yang deficiency, the optimal acupuncture parameters combination should be acupuncture at Shenshu (BL 23), with twisting angle by 90°, twisting frequency at 180/min, and needle twisting time 120 s. Different factors can produce different effects on the therapeutic efficacy of acupuncture in treating rats with kidney yang deficiency; among the factors, the time of needle twisting produces the most significant effect, followed by frequency of needle twisting, angle of needle twisting, and selection of acupoints.

OBJECTIVETo explore the mechanism of natural moxibustion on regulating immune imbalance in asthma rats.

METHODSSeventy SD male rats were divided into a normal group, a placebo group, a dexamethasone group, a big-cake for long-course moxibustion group, a big-cake for short-course moxibustion group, a small-cake for long-course moxibustion group and a small-cake for short-course moxibustion group, ten rats in each one. The rat model of asthma was established by egg albumen sensitization and stimulation in all the groups except the normal group. The natural moxibustion was used in all moxibustion groups, in which big cake of 1 cmX 1 cm size was used in the big-cake groups and small cake of 0.5 cmX 0. 5 cm size was used in the small cake groups. According to relevant acupoints, the natural moxibustion was performed, 5 h per time, once a day. Four times of treatment was considered one course, and three courses were required in the long-course groups and one course was required in the short-course groups. Intraperitoneal injection of dexamethasone was applied in the dexamethasone group, which had the same course as long-course moxibustion group. After the treatment, changes of EOS in peripheral blood of asthma rats were observed; enzyme linked immunosorbent assay (ELISA) was applied to test the contents of IgE, IL-4 and IFN-gamma in the lung tissue; real-time Q-PCR method was adopted to measure the expression level of transcription factor T-bet and GATA binding protein 3 (GATA-3) in the lung tissue.

RESULTSCompared with the normal group, the EOS in whole blood as well as IL-4 and IgE in plasma were all increased in the placebo group (all P< 0. 01), IFN-gamma in plasma was obviously decreased (P<0. 01); while the levels of EOS, IgE and IL-4 were significantly reduced (all P<0. 01), the content of IFN-gamma was increased (P<0. 01) in all moxibustion groups and dexametnasone group. Compared with the normal group, the expression of T-bet mRNA in the placebo group was significantly reduced (P<0. 01). Each treatment group could significantly increase the expression of T-bet mRNA and reduce that of GATA-3 mRNA (P<0. 01). Compared with the short-course moxibustion groups, the expression of T-bet mRNA was obviously increased in the long-course moxibustion group and dexamethasone group (both P<0.01), and that of GATA-3 mRNA was reduced (P<0.01). There was no significant difference between long-course moxibustion group and dexamethasone group (P> 0. 05), and also no significant difference could be seen between big-cake moxibustion group and small-cake moxibustion group (P>0. 05).

CONCLUSIONThe natural moxibustion could obviously reduce airway inflammation in asthma rats. With time passing, the efficacy is enhanced, indicating evident timeliness, which has no apparent relationship with the size of moxibustion cake.

Animals ; Asthma ; genetics ; metabolism ; therapy ; Cytokines ; genetics ; metabolism ; Humans ; Male ; Moxibustion ; Rats ; Rats, Sprague-Dawley ; Transcription Factors ; genetics ; metabolism

Child ; Child, Preschool ; Humans ; Vestibulocochlear Nerve Diseases ; diagnosis ; prevention & control