To evaluate the forensic determination of post cerebral traumatic epilepsy. Methods In 21 patients, traumatic history and previous history were analysied combined with the demonstrations of electroencephalogram(EEG), X-ray, CT and MRI. Results Post-traumatic epilepsy,manily in late stage, usually occurred following serious cerebral trauma. The type of traumatic epilepsy was determined by the traumatic location and extent. Abnormal epileptic wave in scalp EEG and 24h dynamic EEG and medical image examinations were helpful for qualitative analysis. Conclusion The forensic determination of post traumatic epilepsy must be on the basis of traumatic and previous history combined with EEG, CT and MRI analysis.

The serous anti-corneum antibodies(ACAs)of 50 psoriatic patients and 32 normal con- trols were measured by Biotin Avidim Enzyme Linked Immunosorbent Assay (BA-ELISA).The results showed that ACAs of patients were significantly lower than that of the controls(P

Objective: To detect the platelet inhibition of aspirin and Clopidogrel in patients after percutaneous transluminal angioplasty and stenting (PTAS) using a thromboelastograph (TEG) instrument in order to guide the individualized adjustment of anti-platelet aggregation drugs after PTAS. Methods: The clinical data of 65 patients with ischemic stroke or transient ischemic attack included from Nanjing Stroke Registry Program were analyzed retrospectively. Venous blood samples were collected at day 3 after PTAS. A TEG instrument was used to detect arachidonic acid (AA) -induced inhibition rate of platelet aggregation and adenosine diphosphate (ADP) receptor-induced inhibition rate of platelet aggregation. The AA pathway and ADP receptor-induced inhibition rate of platelet aggregation, as well as the response differences of the patients between aspirin and clopidogrel therapy were compared. Results: Circled digit oneThe inhibition rate (80 ± 28%) of aspirin for the AA pathway was significantly higher than that (53 ± 31%) of clopidogrel for the ADP receptor pathway (P < 0.01). Circled digit twoOf the 65 patients, the therapeutic effects in 45 (69.2%), 8 (12.3%), 7 (10.8%) and 5 (7.7%) patients were good, effective, low response, and ineffective in the aspirin group, and those in 19 (29.2%), 14 (21.5%), 23 (35.4%) and 9 (13.8%) were good, effective, low response, and ineffective in the clopidogrel group. Of those who had a good response to aspirin, 3 had no response to clopidogrel and 14 had low response to clopidogrel; of those who had a good response to clopidogrel, all responded well or effective to aspirin. Of those who had low response to clopidogrel, 4 had low response to aspirin, 5 had a good response to aspirin, and 14 had good results. The two efficacies had some relevance (χ2 = 33.311, P < 0.01). Circled digit threeA total of 53 patients had a good + effective response to aspirin, and 12 had low response + ineffective to aspirin, while 33 and 32 patients had good + effective and low response + ineffective to clopidogrel. There was significant difference in efficacy between the two drugs (χ2 = 15. 042, P < 0.01). Conclusion: Using TEG instrument to detect the inhibition rate of platelet aggregation in patients after PTAS is beneficial to guide the development of therapeutic scheme for individualized anti-platelet aggregation in clinical practice. The inhibitory effect of platelet aggregation of aspirin after PTAS is stronger than that of clopidogrel. The patients show differential responses to aspirin and clopidogrel therapy. Some patients who have low response to clopidogrel may respond well or may be effective to aspirin.

OBJECTIVE: To examine the anti-proliferation effects of a novel derivative of dehydroabietylamine, dehydroabietyl-amine-fluorobenzaldehyde[1-(7-isopropyl-1, 4a-dimethyl -1, 2, 3, 4, 4a, 9, 10, 10a-octahydrophenanthren-l-yl)-N- (4-fluorobenzyli-dene) methanamine, DHAA-F]. on human hepatocellular carcinoma cells and to expolore its molecular mechanism. METHODS: MTT assay was adopted to detect the proliferation status of the cells treated with DHAA-F; cellular apoptosis and reduction of mitochondria membrane potential (δφm) were analyzed using flow cytometry (FCM): Western blotting assay was used to evaluate the release of Cyt c, and the expressions of p53, Bcl-2 and Bax protein; and the caspase-3activity was determined with fluorescence spectrophotometry. To evaluate the anti-tumor effect of DHAA-F in vivo, mouse model bearing inoculated H22 tumor was established. RESULTS: DHAA-F strongly inhibited human hepatoma cells proliferation. The IC50 value of DHAA-F was (44.47±2.15) pjnol · L-1 for SMMC-7721, (48.64±1.76) μmol · L-1 for Bel-7402, and (52.83±2.25) μmmol · L-1 for HepG2. DHAA-F displayed a significant inhibitory effect on the growth of SMMC-7721cells in a dose- and time-dependent manner. When SMMC-7721 cells were pretreated with DHAA-F for 24 h, the apoptosis rate significantly increase and the mitochondrial membrane potential significantly decreased. Western blotting assay showed significant decrease of Bcl-2 protein expression and increase of Bax, p53 protein expression, cytosol Cyt c level and caspase-3activity. DHAA-F could significantly reduce tumor weight in the H22 solid tumor mouse model in vivo. CONCLUSION: These findings suggest that DHAA-F has potent antitumor activity both in vivo and in vitro and the mechanism may be related to the apoptosis induced by DHAA-F through a mitochondrial pathway.

Objective To investigate the significance to separate acute and chronic wards in a rehabilitation medicine department of generalhospital. Methods The patients discharged from acute wards from August 2012 to April 2013, when acute and chronic wards has beenseparated, were as group A (n=289), those from chronic wards were as group B (n=108). The patients discharged a year before the separation were as the control (group C, n=402). Their activities of daily living (ADL) when discharged, satisfaction and the time in the acute wards were observed. Results There was no significant difference in ADL between group A and group C (P>0.05), but it was between group B and group C (P<0.05). The patients more satisfied after separation (P<0.05), while the time in the acute wards decreased. Conclusion Separating the acute and chronic wards may improve the use of the medical resources and serve more patients. However, it improve the quality of care and satisfaction of the patients.

Objective To promote rational drug use by investigating quality of the outpatient and emergency prescriptions in the hospital .Methods We statistically summarized the evaluations of prescriptions and analyzed the drug use from January to Novem-ber ,2012 .Results The outpatient and emergency prescriptions were compliant to relevant writing standards ,without missing i-tems .The inaptitude of prescriptions were mainly in forms of inappropriate indication ,usage ,and dosage of drug and menstruum . The irrational prescriptions accounted for 5 .5% of those evaluated .Conclusion Rational drug use has been basically ensured through implementation of prescription evaluations and interventions .Irrational prescriptions exist in relatively small scale and are to be eliminated .

Objective To evaluate the effect of the Danhong injection combined with irbesartan in treatment ofearly diabetic nephropathy. Methods 64 patients with early diabetic nephropathy were randomly divided into two groups and the control group 32 cases,two groups were given control of blood sugar and ensure water and electrolyte balance,support therapy,in which 32 cases were also observed red Danhong injection,Ecuador irbesartan treatment,2weeks before evaluation. Results The total effective rate in treatment group was higher than the control group( x2 =6.38 ,P ＜0. 05) ,after 12 weeks of treatment,two groups of urinary protein decreased significantly compared with before treatment, including observation of urine protein was ( 0. 6± 0. 1 ) g/24h, the control group was ( 0. 7 ± 0. 3 ) g/24h,and decreased urine protein level of the observation group was better than the control group( t = 2. 74,P ＜ 0. 05 ). And in the course of treatment, side effects and serious adverse events were not found. Conclusion Danhong injection combined irbesartar, could improve the clinical symptoms, reduce microalbuminuria in treatment of early diabetic nephropathy is worthy of wider application.

Objective To observe the connections between enkephalin immunoreactive(ENK-ir) terminals and ?-aminobutyric acid immunoreactive(GABA-ir) neurons in the rostral portion of the nucleus tractus solitarious(rNTS) of the rat. Methods Double-immunofluorescent labeling method and the pre-embedding immunohistochemical staining combined with immuno-gold particles labeling technique for electron microscopic detection were used in the present study. Results Under the laser scanning confocal microscope,dense ENK-ir fibers and terminals and some GABA-ir neurons were observed in the rNTS.Close contacts between ENK-ir terminals and GABA-ir cell bodies were also detected.By using the electron microscopic technique,ENK-ir reaction products were found to be mainly localized on the surface of round clear vesicles and dense-cored vesicles in the axon terminals.ENK-ir terminals formed symmetric,which was a dominant pattern,and asymmetric synaptic connections with GABA-ir and immunonegative cell bodies and dendrites.Conclusion The ENK-ir terminals might take part in the transmission and regulation of the taste information in the rNTS through inhibiting,exciting the GABAergic neurons or inhibiting directly the activity of neurons within the rNTS.

To realize long-term long distance space exploring flight,the bioregenerative life support system has to be applied.Producing animal protein in this system could increase its closure ability and stability.In this paper,researches and developments on the animal protein production in the bioregenerative life support system from various countries including our research results were introduced in detail through information analyses.The prospects in the future studies were also predicted.It has important references for our country to carry out related investigations.