Objective:To analyze the anti-infection therapy for one lung cancer patient with agranulocytosis and fever after chemo-therapy to provide pharmaceutical care and support for the rational anti-infection drug use in the patient. Methods: Clinical pharma-cists analyzed the drugs used in the patient and provided pharmaceutical care for doctors according to the examination results and diag-nosis of the patient. Results:By participating in the treatment plan, clinical pharmacists ensured the treatment for the patient was safe and effective. Conclusion:Clinical pharmacists should assist doctors in making out the treatment plan according to patients’ situation in order to promote rational drug use in clinics and reflect the value of pharmaceutical care.

Administration, Oral ; Anti-Inflammatory Agents, Non-Steroidal ; adverse effects ; Female ; Hematuria ; chemically induced ; Humans ; Infant ; Male ; Sulfonamides ; adverse effects

Objective To investigate the current status analysis the risk factors of medical adhesive related skin injury(MARSI) in neonates in NICU, in order to provide guidance in prevention and nursing of the skin injury. Methods Convenience sampling five NICU in Shenyang, prospective investigate the incidence of MARSI of neonates during the NICU stay, and analysis the incidence , injury type distribution and risk factors. Results The incidence of MARSI in neonates in NICU was 12.8%(53/413);the major injury type of MARSI was epidermal stripping(47.2%,25/53)and skin tear(35.8%,19/53) in mechanical injury;Logistic analysis showed that the independent risk factors of MARSI were the use of stomach tube, dehydrate skin and blue light therapy (χ2=5.953, 9.806 and 7.568, P<0.05). Conclusions MARSI is frequency interrupted in neonates during the NICU stay, correct selection of medical adhesive products, application of suitable paste and remove skills are helpful to reduce the incidence of MARSI in neonates during the NICU stay.

Objective The present study was undertaken to investigate the properties of nonlinear dynamics of EEG and the changes in depth of anesthesia with real-time approximate entropy (ApEn) and complexity (Cx) nonlinear indexes monitoring during anesthesia. Methods EEG was recorded in 65 in-patients. They were randomly divided into 4 groups: isoflurane, sevoflurane, desflurane (n=15, respectively), and propofol intravenous anesthesia (n=20) groups. The EEG derived parameters ApEn and Cx non-linear indexes were calculated simultaneously during the whole operation including rest state with eyes closed, anesthetic induction, intraoperation, recovery, post-operation awaking. Results ApEn and Cx nonlinear indexes remained the highest during rest state. Both of them kept decreasing during anesthetic induction. They dropped to a relative lower value and leveled off in the intra-operation period. Both of them rose gradually during recovery period and returned to a high level in the post-operation awaking period (correspondingly, ApEn: 0.87, 0.78, 0.55, 0.64 and 0.83. Cx: 0.58, 0.54, 0.38, 0.46 and 0.57). Conclusions With ApEn and Cx non-linear indexes, changes in depth of anesthesia from EEG signal could be real-timely monitored and precisely measured. Nonlinear dynamic analysis might provide us with more information about consciousness and cognition during general anesthesia.

Background and purpose:Immunity function is one of the most profound factors in affecting the prognosis of breast cancer patients. Cytotoxic T lymphocytes counts in the peripheral blood and focal tumor tissue could indicate the overall survival time of these patients. On the other hand, adjuvant chemotherapy is also an important part in improving both the disease free survival and overall survival time of breast cancer patients. Selecting chemotherapy regime which is both able to kill all the tumor cells and reserve the immunity function to the greatest extent is of great importance in improving the survival rate of breast cancer patients. The aim of this study was to compare the effect of two chemotherapy regimens CEF (cyclophosphamide, epirubicin and lfuorouracil) and EC followed by P (paclitaxel) on the peripheral blood lymphocytes in early stage breast cancer. Methods:The clinicopathological characteristics and peripheral blood lymphocyte parameters before and after chemotherapy of CEF or EC-P regimen were retrospectively analyzed in post-operate patients with early stage breast cancer during the period from Nov. 2012 to May 2013. The lymphocyte parameters included: total blood lymphocytes count, percentages of T lymphocytes, cytotoxic T lymphocytes, helper T lymphocytes, active T lymphocytes and nature killer (NK) cells. Results: Patients undertook EC-P regimen were those at comparably high risk (signiifcant differences of clinical stage, tumor size, axillary lymph node status, estrogen/progestogen receptor and histological subtype were observed). There was no difference of lymphocyte parameters between these two groups before adjuvant chemotherapy. However, during the process of chemotherapy, peripheral blood lymphocytes counts decreased signiifcantly after 4 and 5 cycles of chemotherapy of CEF regime (1 077±359/μL;1 181±271/μL) compared with the level before chemotherapy (1 607±322/μL, P<0.05). On the contrary, there was no signiifcant difference of peripheral blood lymphocytes count before (1 746±576/μL) and after 4 and 5 cycles of chemotherapy (1 500±312/μL;1 623±468/μL) in EC-P group (P>0.05). Percentage of active T lymphocyte increased signiifcantly along with the chemotherapy in both groups (CEF group:11.8±7.1 vs 23±9.3, P<0.05;EC-P group:11.8±5.8 vs 17.6±8.2, P<0.05) (pre-chemotherapy vs after 5 cycles of chemotherapy). In EC-P group, the percentage of helper T lymphocyte (37.8±5.7) decreased significantly compared with the levels before chemotherapy (41.3±4.3) and before paclitaxel was undertaken (41.9±5.6, P<0.05) and the percentage of NK cells (21.5±5.2) increased significantly compared with the levels before chemotherapy (15.3±7.6) and before paclitaxel was undertaken (14.9±5.9, P<0.01) after one cycle of paclitaxel therapy. Conclusion:The effect of chemotherapy on peripheral blood lymphocyte is less profound in EC-P group compared to CEF group. Furthermore, paclitaxel can increase the NK cells without any effect to the levels of T lymphocytes and cytotoxic T lymphocyte. It is superior over other drug in conserving immune function in early stage breast cancer.

Objective: To identify serum autoantibody biomarkers by an tumor-associated antigens (TAAs)-based microarray in the patients with esophageal precancerous lesions (EPLs) and patients with esophageal cancer (EC). Methods: The antigens microarray with 55 TAAs was used to detect the serum autoantibodies of the residency-, age-, and sex-matched 26 pairs, which included 26 cases with EC, 26 cases with EPLs and 26 normal controls. The cut-off value was defined as mean ± 2 standard deviation of the expression levels of the antibodies in the normal control group. The value corresponding to the expression level of the antibody beyond the range of cut-off value was considered seropositive. Results: Five autoantibodies (CYFRA21-1, CA72-4, NY-ESO-1, GAGE-7 and SCCA) were significantly up-regulated, and others were down-regulated. Twenty-five autoantibodies had significant difference among EC, EPLs and normal controls which were found by multi-factor analysis of variance. Serum antibodies could be detected by 9 antigens, including CA72-4, CCNB1, CDKN2A, NY-ESO-1, CYFRA21-1, E2F1, ERBB2, GAGE-7 and SCCA. The positive detection rates of CYFRA21-1 were the highest in EC and EPLs, which were 61.54% and 60.00%, respectively. Furthermore, other antigens with higher seropositive rates were NY-ESO-1 (50.00% for EC, 52.00% for EPLs), SCCA (46.15% for EC, 48.00% for EPLs), GAGE-7 (46.15% for EC, 24.00% for EPLs) and CA72-4 (34.62% for EC, 16.00% for EPLs) in turn. The seropositive rates of these 9 antigens in combined detection panel were 88.46% and 84.00% for EC and EPLs, respectively. Conclusion: CYFRA21-1 is possibly used as an autoantibody indicator for early screening of EC and EPLs. The combined detection of the 9 antigens is likely to improve the sensitivity of autoantibody detection in EC and EPLs serum samples. Copyright © 2013 by TUMOR.

Objective: To explore the extraction process of Trollii Flos Oral Liquid (TFOL). Methods: HPLC was used to determine orientin and vitexin in liquid extract of TFOL, using mass concentration of both as index. The orthogonal design L18(37) and reverse continuous cycle cryogenic extraction were used to extract Trollii Flos, the optimum extraction was determined, compared with the current standard of TFOL extraction process. Results: The optimum extraction conditions of reverse continuous cycle cryogenic extraction were as follows: extraction temperature 90°C, extraction time 2 h, powder type 10 mesh, added of 20 times water. The linear ranges of orientin and vitexin were 18.46-295.37 (r = 0.9999) and 5.84-93.44 μg/mL (r = 0.9999), the average recoveries of orientin and vitexin were 96.07%, RSD = 0.74% (n = 6), and 97.73%, RSD = 1.42% (n = 6), respectively. Conclusion: The technology of reverse continuous cycle cryogenic extraction is stable and feasible, and the extraction rate is high; The measurement method is accurate, sensitive, simple, and stability, and has good reproducibility. The extraction and determination methods could be used as production process and quality control reference of TFOL.

Objective To identify the effect of specialized language training on the speech rehabilitation of total laryngectomy (TL)patients.Methods Twenty-seven TL patients were trained for esophageal speech by specialized speech-language pathologists from October 2005 to December 2006.Then the participants were followed and evaluated their esophageal speech level after one cycle of training program,1 month,3 months,6 months and 12 months after training program.Results The score of participants' esophageal speech was steadily improved during the 12 months after participating the training program.The score of esophageal speech greatly increased in the third month,and this significant improvement was kept until 12 months after the training program.The esophageal speech quality was negatively correlated with age and surgery area of patients.Conclusions Specialized speech-language training showed positive effect on esophageal speech rehabilitation,and esophageal speech training by specialists is worthy of wide application.

OBJECTIVETo explore the protective effect and mechanism of total flavones of Bidens pilosa L. (TFB) on IgA1 induced injury of venous endothelial cells in Henoch-Schönlein purpura (HSP) children patients. METHODS Human umbilical venous endothelial cells (HUVECs) were taken as subject. They were intervened by normal IgA1 and HSP children patients' serum IgA1, and added with different concentrations TFB at the same time. Then they were divided into the blank control group, the normal control group, the HSP IgA1 group, and HSP IgA1 plus TFB (1.0, 0.5, 0.25 mg/mL) groups. Levels of TNF-α and IL-8 in supernate were detected by ELISA. The NO level was detected by nitrate reductase method. mRNA and protein expressions of NF-κB and ICAM-1 in HUVECs were detected by fluorescent quantitative PCR and Western blot respectively.

RESULTSCompared with the normal control group and the blank control group, levels of IL-8, TNF-α, and NO all significantly increased in the HSP group (P < 0.05). Compared with the HSP group, levels of IL-8, TNF-α, and NO significantly decreased after intervention of TFB (1.0 and 0.5 mg/mL; P < 0.05, P < 0.01). Results of fluorescent quantitative PCR and Western blot showed, as compared with the blank control group and the normal control group, mRNA and protein expressions of NF-κB and ICAM-1 in HSP children patients' serum IgA1 induced venous endothelial cells significantly increased with statistical difference (P < 0.05, P < 0.01). Compared with the HSP group, mRNA and protein expressions of NF-KB and ICAM-1 were obviously down-regulated after intervention of TFB (1.0, 0.5, 0.25 mg/mL), with statistical difference (P < 0.05, P < 0.01).

CONCLUSIONTFB could protect vascular damage by inhibiting in vivo high expression of NF-κB, reducing the production of IL-8, TNF-α, and NO in vascular endothelial cells of HSP children patients.

Bidens ; chemistry ; Child ; Flavones ; pharmacology ; Human Umbilical Vein Endothelial Cells ; drug effects ; Humans ; Immunoglobulin A ; blood ; Intercellular Adhesion Molecule-1 ; metabolism ; Interleukin-8 ; metabolism ; NF-kappa B ; metabolism ; Nitric Oxide ; metabolism ; Purpura, Schoenlein-Henoch ; blood ; RNA, Messenger ; metabolism ; Tumor Necrosis Factor-alpha ; metabolism

Child ; Congresses as Topic ; Humans ; Pediatrics