Objective:To explore the pretection of small dosage desamethasone to pulmonary function on acute lung injury model.Methods:54 male SD rats were divided randomly into control group,injury group and therapeutic group.Major observed marker consisted of count of cell sum,total protein level and TNF-? level of bronchoalveolar douche solution.Secondary marker consisted of lung coefficient,PAO2,TNF-? level in blood serum and histopathologic examination.Results: Alveolar texture in therapeutic group existed yet,but alveolar interval increased thickness,neutrophilic granulocyte infiltration relieved obviously,and a little bleeding in alveolar cavity.PAO2 in therapeutic group was higher than that of injured group(P

Congenital adrenal hyperplasia(CAH) is a family of autosomal recessive disorders.The most frequent form of the disease is steroid 21-hydroxylase deficiency,which accounts for more than 90％ of all cases of CAH.In the pathogenesis of CAH,the cortisol synthesis impairement leads to excessive ACTH secretion of the adrenal glands,which ultimately results in adrenal hyperplasia and androgen overproduction.CAH causes signs of androgen excess including ambiguous genitalia in newborn females and rapid postnatal growth in both sexes.In the most severesalt wasting form of CAH,concomitant aldosterone deficiency may lead to salt wasting with life-threatening adrenal crises.Newborn screening,especially in males,reduces morbidity and mortality from adrenal crises.Glucocorticoid replacement remains the cornerstone of treatment.However,how to make a suitable therapeutic regimen of CAH is still a challenge to the pediatric clinician.

Objective To investigate the effect of Sufentainil on myocardial ischemia-reperfusion injury and caspase-3 to rat heart. Methods Ninety Wistar rats weighing 200-300g were randomly divided into 3 groups(n=30):sham group(group S), ischemia-reperfusion group (group I/R)and Sufentainil group (group SF). Each group was divided into 3 subgroups according to the time phases at 30, 60 and 120min(n=10).In group I/R and group S the animals were received normal saline intravenously. In group SF sufentainil 5μg/kg NS 20ml was given. After 24 h, all animals were anesthetized with intraperitioneal 20% urethane 1 mg/kg, intubated and mechanically ventilated. The chest was opened and heart exposed via thoracotomy.In group S , left artery anterior descending artery(LAD) was not ocluded. While in I/R and SF group myocardial ischemia was introduced by clamping LAD for40 min. The animals were killed at 30, 60 and 120 min of reperfusion respectively(n=10each). Cardiac tropoin I(cTnI), expression of caspase-3 and apoptosis of cell in heart tissues were measured at the same time.Ultrastructure of myocardium taking the from apex was examined by electron microscopy at the end of 120 min reperfusion. Results Compared with group S, the concentration of cTnI was increased in the other 2 groups(P<0.05).cTnI was significantly lower in group SF than in group I/R(P<0.05). Expression of caspase-3 and apoptosis index induced by myocardial reperfusion were attenuatated in group SF(P<0.05). The damage of myocardial ultrastructure caused by myocardial I/R were also significantly improved in group SF. Conclusion Sufentainil may relieve the heart cellular apoptosis by decreasing caspase-3expressing.

Objective To study the relationship between HLA-DRB1alleles and the systematic lupus erythematosus of the Zhuang Nationality in Guangxi Province.Methods Allele polymerase chain reaction-sequence specific primers(PCR-SSP)were used to study the HLA-DRB1allele genes in52patients with SLE and70healthy controls of the Zhuang Nationality in Guangxi Province.Results The allele frequencies of HLA-DRB1*1401and HLA-DRB1*16were significantly less in SLE patients than those of the controls(RR=0.28,? 2 =5.00,P=0.02and RR=0.39,? 2 =3.95,P=0.05).HLA-DRB1*08,DRB1*11and DRB1*13alleles were not found in these two groups.Conclusion Our data suggest that the HLA-DRB1*1401and HLA-DRB1*16might be protecting genes for SLE in Guangxi Zhuang nationality.No susceptible gene is found in our study.

Apoptosis ; Humans ; Periodontitis ; pathology

Objective To detect the in-vitro microdialysis recovery of nicotine by decrement method(i.e.retrodialysis) and to investigate its influence factors,thus to supply experimental evidence for the in-vivo microdialysis of nicotine.Methods The in-vitro recovery of nicotine was detected by concentration difference method(increment method and decrement method).The stability of recovery was observed.The effects of the pretreatment with perfusate,and the flow rates and concentrations of the perfusate on nicotine recovery were studied.Results Filtering and stirring treatment increased the recovery,but the time of ultrasound degassing showed no effect on the recovery.The recovery detected by increment method was as the same as that detected by decrement method.Nicotine recovery was independent of nicotine concentration in the external medium,showing good stability.Conclusion It is necessary to filter and degas the perfusate before microdialysis.Retrodialysis can be used for the determination of in-vivo nicotine recovery.

Diabetic retinopathy ( DR ) is one of the main blinding eye diseases for people over the age of 50, and diabetic macular edema ( DME) is the leading cause of vision loss is DR patients. The early diagnosis and early treatment is important. As OCT and FFA, mfERG, especially the retinal thickness, volume, retinal edema index quantitative indicators such as objective evaluation of macular edema, embodies the new progress of retinal imaging technology in recent years. OCT is a non -contact clinical application in recent years, noninvasive, high resolution of ophthalmic imaging examination, can do it on retinal ultrastructure observation and quantitative analysis, and the technology is relatively mature, become a routine inspection diagnosis of macular edema. Laser photocoagulation, intravitreous injection with Ranibizumab and vitrectomy is nowadays the important means for the treatment of intractable macular edema.

Capsid Proteins ; metabolism ; Endocytosis ; physiology ; Virus Internalization ; Viruses ; metabolism

Adolescent ; Adult ; Child ; Child, Preschool ; Heart Defects, Congenital ; surgery ; Humans ; Young Adult

Bone Neoplasms ; Female ; Humans ; Middle Aged ; Waldenstrom Macroglobulinemia