【Objective】 To sequence P1 gene(184～1946 bp) includ ing SBR gene in expressional plasmid of transgenic plant. 【Methods】 pROP1 plas mid was extracted from E. coli DH5α, P1 gene was sequenced by PCR sequence kit and DNA autosequencer and compared with surface protein gene of Streptococc us mutans in GenBank. 【Results】 616 base pairs from 5′ end and 466 base pairs from 3′ end were sequenced, its accuracy is up to 98%. 【Conclusion】 The seq uence of P1 gene's 5′ end and 3′ end is consistent with DNA sequence of SBR re gion of Streptococcus mutans surface protein pac gene and may provide useful in formations to construct the transgenic plant anticaries vaccine.

Objective To study the effect of Danshen injection on brain peptide type B (NT‐proBNP) in patients with hyperten‐sion and left ventricular failure ,provide an effective basis for clinical treatment .Methods Totally 80 patients of hypertension with left heart failure from February 2015 to February 2016 were randomly divided into two groups :40 patients were treated with con‐ventional therapy (control group ) ,40 patients in the conventional treatment combined with Danshen injection (experimental group) .We compared NT‐proBNP ,inflammatory factors and clinical efficacy between two groups .Results Before treatment ,the expression of NT‐proBNP and hs‐CRP ,NT‐proBNP level was basically the same in two groups (P> 0 .05) .After treatment ,the ex‐perimental group of patients with NT‐proBNP was significantly lower than the control group and the LVEF ,left ventricular mass index and left ventricular volume index were significantly higher compared with the control group (P< 0 .05) .The total effective rate of patients in Danshen injection groups was significantly higher (P< 0 .05) .Conclusion The injection of Danshen in patients with hypertension and heart failure patients can significantly improving NT‐proBNP levels and improve the cardiac function .

OBJECTIVE:To compare the relative bioavailability between2loratadine tablets(LORAX and LORAS)in human body.METHODS:22healthy male volunteers were enrolled to receive oral single dose(20mg)of either LORAX or LORAS tablets by crossover way,plasma concentrations of loratadine were determined by HPLC and pharmacokinetic param-eters were calculated by3p97program.RESULTS:The pharmacokinetic parameters of LORAX vs.LORAS were the fol-lowing,t 1/2Ke (3.03?0.98)h vs.(2.65?0.81)h;C max (8.59?6.70)vs.(9.59?8.22)ng/ml;t max (1.08?0.41)h vs.(1.21?0.28)h;AUC (0～t) (27.29?24.54)vs.(27.92?20.64)(ng?h)/ml.The relative bioavailability of LORAX was(100.63?14.27)%.CONCLUSION:2loratadine tablets were bioequivalent.

Diabetic retinopathy is a progressive vision-threatening complication of diabetes mellitus.The characters of diabetic retinopathy in clude capillary occlusion,microcirculation disturbance and retina neovascularization near ischemic areas of the retina.The exact pathogenic mechanism is still unclear.Recent studies showed that it may be related to vascular inflammation of the retina.Cytokines can cause induction of proinflammatory and adhesion molecules and thereby increase monocyte-endothelial cell adhesion,which is now accepted as the early key event in the development of diabetic retinopathy.The functions of many cytokines such as vascular endothelial growth factor,transforming growth factor-β,basic fibroblast growth factor and ideas for medicine therapeutic to diabetic retinopathy are summarized in this review.

Rituximab ( RTX) is a monoclonal antibody to the CD 20 antigen on B-cells that was initially designed and approved for the treatment of non-Hodgkin′s B-cell lymphoma and has emerged as a potent immunosuppressant for many immune -mediated disea-ses.Given its efficacy , tolerability and safety profile in comparison to more conventional treatment regimens , RTX is rapidly emerging as a critical therapeutic modality for glomerular disease .The paper reviews the role of RTX in the treatment of ANCA-associated vascul-itis, lupus nephritis , mixed cryoglobulinemia , membranous nephropathy , minimal change disease , and focal segmental glomeruloscle-rosis.

Objective To observe the clinical effect of card other tromethamine for postpartum hemorrhage. Methods 80 cases with postpartum hemorrhage were randomly divided into the observation group and control group, 40 cases in each group.The control group was given oxytocin treatment.While the observation group was given oxyto-cin and card other tromethamine treatment.The clinical curative effect,bleeding time after treatment,postpartum 2h haemorrhage amount and adverse reaction,etc.were observed and compared.Results The total effective rate of the observation group was 95.0%,which was significantly higher than 75.0% of the control group,the difference was sta-tistically significant(χ2 =15.68,P <0.05).The haemorrhage amount of postpartum 2h of the observation group was (273.7 ±10.5)mL,which was significantly lower than (547.1 ±16.4)mL of the control group,the difference was statistically significant(t =88.79,P <0.05).The bleeding time of the observation group was (14.8 ±3.4)min,which was significantly shorter than (34.9 ±8.4)min of the control group,the difference was statistically significant (t =14.02,P <0.05).The incidence rate of adverse reactions of the observation group was 7.5%,that of the control group was 5.0%,there was no statistically significant difference between the two groups(χ2 =0.53,P >0.05 ). Conclusion Card other tromethamine in the treatment of postpartum hemorrhage has good clinical curative effect and high safety.

Objective:To investigate the association of tumour necrosis factor- ? (TNFs-?) gene polymorphism with Graves ophthalmopathy (GO)Chinese in Shandong . Methods: Allele and genotype frequencies were determined by polymerase chain reaction with sequence specific primers(PCR-SSP). Results: Allele and genotype frequencies of TNF-? +488、-308 were not different significantly between Graves′ disease(GD) and control, or GD subgroups with and without GO. There were also no significant differences between GO subgroups with different severity. Conclusion: There are no associations of TNF-? +488、–308 polymorphisms with GO susceptibility in Shandong Chinese.

Antineoplastic Combined Chemotherapy Protocols ; administration & dosage ; Carcinoma, Non-Small-Cell Lung ; drug therapy ; Humans ; Lung Neoplasms ; drug therapy