AIM: To explore the clinical significance of Krüpple-like factor 15 (KLF15) protein expression in the patients with lung adenocarcinoma for exploring the therapeutic and prognositic biomarkers of lung cancer.ME-THODS: Four cases of lung adenocarcinoma tissues and matched adjacent tissues were collected from our hospital, and the expression of KLF15 protein in these tissues was analyzed by Western blot.At the same time, 72 cases of archived paraffin-embedded samples and clinical data of the patients with lung adenocarcinoma were also collected.The KLF15 protein expression in the archived paraffin-embedded lung adenocarcinoma samples was detected by immunohistochemical staining.The correlations between KLF15 protein expression and clinical characteristics of the patients including prognosis were also analyzed.In addition, the KLF15 protein was up-regulated in A549 cells, and then the effects of KLF15 protein on the viability of the cells were measured by CCK-8 assay.RESULTS: The protein expression of KLF15 in the 4 cases of lung adenocarcinoma tissues was significantly lower than that in matched paracancerous tissues.Fifty-three cases of lung adenocarcinoma specimens showed low expression or no expression of KLF15 protein in total 72 cases (73.6%).The 5-year survival rate of the patients with high expression of KLF15 protein in their specimens was higher than that of the patients with the low expression of KLF15 protein (P<0.01), and the expression of KLF15 protein was significantly correlated with the pathological staging (P<0.01) and T stage (P<0.01) of the patients with lung adenocarcinoma.Furthermore, the low expression of KLF15 protein was an important poor prognostic indicator of the patients.Up-regulation of KLF15 protein in the A549 cells significantly inhibited the growth of the cells.CONCLUSION: KLF15 inhibits the growth of lung adenocarcinoma cells.It could be used as a therapeutic target and a prognostic biomarker for the patients with lung adenocarcinoma.

Objective To investigate the prognosis of middle-aged and elderly patients with aspiration pneumonia and related influencing factors in integrated medical and elderly care institutions. Methods A total of 604 elderly patients with aspiration pneumonia were selected from integrated medical and elderly care institutions from January 2016 to December 2020. The prognosis of the patients was counted, and the prognostic factors were analyzed by univariate and multivariate logistic regression equations. The receiver operating characteristic (ROC) curve and area under the curve (AUC) were used to analyze the predictive value of logistic regression model for the prognosis of elderly patients with aspiration pneumonia. Results Univariate analysis showed that Alzheimer's disease, severe pneumonia, ability of daily living (ADL), and the number of rescues were related to the prognosis of elderly patients with aspiration pneumonia (P<0.05). Logistic regression model showed that the death risk of patients with Alzheimer's disease was 2.883 times higher than that of patients without Alzheimer's disease, the death risk of patients with severe pneumonia was 3.292 times higher than that of patients without severe pneumonia, the death risk of patients with severe dependence in ADL was 3.719 times higher than that of patients with mild dependence, the death risk of patients with moderate dependence in ADL was 2.558 times higher than that of patients with mild dependence, and the death risk of patients with rescue times ≥ 2 times was 2,922 times higher than that of patients without rescue times (P < 0.05). Logistic regression equation: logistic (P=-8.264+2.883)×Alzheimer disease ≥60 years old + 3.292×severe pneumonia + 3.719×severe dependence in ADL+2.558×moderate dependence in ADL +2.922× number of rescues. The predicted prognosis AUC of elderly aspiration pneumonia was 0.907 (95%CI: 0.848-0.966). When logistic(P>12.97), the predictive value was the best, with the predictive sensitivity being 83.87%, and the specificity being 84.15%. Conclusion Alzheimer's disease, severe pneumonia, moderate and severe dependence in the ability of daily living and rescue times ≥ 2 are related to the prognosis of middle-aged and elderly patients with aspiration pneumonia in the integrated medical and elderly care institutions, which can be used as a prognostic plan for clinical treatment and nursing management. At the same time strengthening the intervention of patients with the above characteristics will help to promote the improvement of prognosis.