1.The effect of astragalus polysaccharides on androgen excess within ovarian granulosa cells of PCOS patients in vitro
Yajuan ZHANG ; Min SANG ; Hongxiu ZHANG ; Linan LI ; Huiming LV ; Yuan AN
Journal of Chinese Physician 2011;13(12):1607-1610
ObjectiveTo study androgen excess within ovarian granulosa cells of PCOS patients in vitro,and the partial improvement by using astragalus polysaccharides.MethodsOvarian granulosa cells from the PCOS patients during IVF / ICSI - ET were isolated and cultured in vitro,then it was treated by different concentrations of astragalus polysaccharides.The levels of estradiol,progesterone and testosterone were determined.ResultsAndrogen secretion within ovarian granulosa cells from PCOS patients was significantly increased [ ( 0.4716 ± 0.03 ) nmol/L,( 0.23 ± 0.0162 ) nmol/L,P < 0.05 ].The levels of estradiol and progesterone were not significantly different( P > 0.05 ).Different concentrations of astragalus polysaccharides may result from the alleviation of androgen secretion which was commonly found in PCOS treatment (concentration was 1000 μg/ml,100 μg/ml,50 μg/ml,P < 0.01 ;concentration was 500 μg/ml,P =0.002 ; concentration was 200 μg/ml,P =0.003).ConclusionsAstragalus polysaccharides inhibit the secretion of testosterone in granulosa cells of PCOS patients during in vitro,and it may be an alternative treatment for PCOS.
2.Effect of Early Rehabilitation on Inflammatory Response after Acute Cerebral Infarction
Zhaoru GAN ; Lihua SHI ; Chaoqun CAI ; Zhiguang XIE ; Yingmei XU ; Haipeng LIU ; Linan SANG
Chinese Journal of Rehabilitation Theory and Practice 2010;16(9):807-808
ObjectiveTo observe the effect of early rehabilitation on motor function, high-sensitivity C-reactive protein (hs-CRP), interleukin-1(IL-1) and interleukin-6(IL-6) in patients with acute cerebral infarction (ACI). Methods160 patients with ACI were randomly divided into rehabilitation group (n=80) and control group (n=80), who accepted early rehabilitation and routine medicine or medicine only, respectively. They were assessed with modified Edinburgh-Scandinavian Stroke Scale (MESSS) and their peripheral level of hs-CRP, IL-1 and IL-6 were detected before and 1, 2, 4, and 8 weeks after treatment. Results4 and 8 weeks after treatment, the level of hs-CRP, IL-1 and IL-6 were lower in rehabilitation group than in control group(P<0.05), as well as the scores of the MESSS (P<0.01). The incidence of improvement is more in rehabilitation group than in control group(P<0.05). ConclusionEarly rehabilitation can reduce the expression of hs-CRP, IL-1 and IL-6, and improve the neurological function in patients with ACI.
3.Value of ultrasound combined with pathological parameters in predicting axillary lymph node metastasis in breast cancer
Tian SANG ; Xuegang REN ; Ye WANG ; Yuwen CAO ; Qiaoli LI ; Linan SHI ; Wenxiao LI ; Jun LI
Chinese Journal of Ultrasonography 2022;31(8):691-697
Objective:To evaluate the value of conventional ultrasound, shear wave elastic parameters and immunohistochemistry in predicting axillary lymph node metastasis of breast cancer.Methods:The ultrasonographic features and pathological results of 172 masses in 152 breast cancer patients who underwent surgery in the First Affiliated Hospital of Shihezi University Medical College from May to October 2020 were analyzed retrospectively. The patients were divided into metastatic group and non-metastatic group according to the status of axillary lymph nodes. The conventional ultrasound characteristics, shear wave velocity (SWV) and immunohistochemical indexes (ER, PR, HER-2, Ki-67) of 2 groups of breast cancer masses were analyzed. Finally, the parameters with statistically significant difference between groups were selected and the Logistic regression model was established.Results:There were significant differences in the aspect ratio, calcification, SWVmean and HER-2 expression between metastatic group and non-metastatic group (all P<0.05). A prediction model was constructed with aspect ratio >1, calcification, high SWVmean and HER-2(+ ). The area under receiver operating characteristic curve (AUC) of the subjects was 0.891, which was larger than the single parameter (all P<0.05), and was in good agreement with pathological results (Kappa=0.731). Conclusions:The joint prediction model can be used to predict the status of lymph nodes, and the axillary lymph node metastasis is more likely to occur in breast cancer with the aspect ratio >1, calcification, high SWVmean and HER-2(+ ).