Objective To investigate the clinical effect of flupentixol and melitracen tablets combined with macrogol 4000 powders on treatment of irritable bowel syndrome with constipation (IBS-C) in elderly patients. Methods Fifty paients aged from 65 to 95 years with IBS-C were randomly divided into 2 groups: control group (23 patients) and treatment group (27 patients). The patients in treatment group received flupentixol and melitracen tablets (1 tablets, once a day) combined with macrogol 4000 powders (1 bag/time, 2 times a day) , while the patients in control group received macrogol 4000 powders alone. The treatment period was 4 weeks. The curative effect, the scores of self-rating depression scale (SDS) and self-rating anxiety scale (SAS) and adverse reaction were compared between two groups. Results At the improvement of depressive symptoms, the effective rate in treatment group and control group was 88.9%(24/27) and 39.1%(9/23), and there was significant difference (P<0.01). At the improvement of anxiety symptoms, the effective rate in treatment group and control group was 74.1%(20/27) and 26.1%(6/23), and there was significant difference (P<0.01). The total effective rate in treatment group and control group was 88.9%(24/27) and 65.2%(15/24), and there was significant difference (P<0.05). Conclusions Flupentixol and melitracen tablets combined with macrogol 4000 powders on treatment of aged IBS-C patients have more distinguished therapeutic effect in improving constipation symptoms, stools times, anxiety and depression.

Hepatocyte growth factor participates in many gynecologic and obstetric diseases such as gynecological tumor,endometriosis and hypertensive disorder complicating pregnancy by combination with its receptor c-Met.This article reviews the expression and mechanism of HGF in these diseases.

Obiective To investigate the effects of antidepressant therapy on whole regulating function of serotonin and tryptophan hydroxylase 2 system in ovariectomized(OVX) rats with depressive disorder.Methods seventy-two female Sprague-Dawley rats were used as subjects.The rats were randomly divided in-to 8 groups as following:Sham operation group (Sn),depression group (Dn),the OVX group (On),OVX with depression group (ODn),OVX with depression + sertraline group (ODs),OVX with depression + citalopram group(ODx),OVX with depression+ venlafaxine group(ODw),OVX with depression+ reboxetine group(0Dr).Rats in group OVX and group OVX with depression were ovariectomized.Rats in group with depression were exposed to the chronic unpredictable mild stress (CUMS).Open-field test and sugar consumption experiment were tested on all rats before the experiment and the 28th day of experiment.The expression of TPH2 mRNA was measured with RT-PCR,and the expression of TPH2 protein was detected by Western blot.The concentration of 5-HT was detected by ELISA.Results (1) After exposure of CUMS,the scores of motion and percentage of sugar consumption experiment in Dn group were decreased contrast to the Sn group (P＜0.05),and the scores in ODn group were decreased compared with the on group(P＜0.05).(2)The protein and gene expression of THP2 in On and Dn group were decreased contrast to the Sn group (P＜0.05).The protein and gene expression of THP2 in ODn were decreased compared with the on group (P＜0.05).The protein and gene expression of THP2 in ODs (protein (0.68±0.07);gene (0.87±0.09)),ODx (protein (0.71 ±0.05);gene (0.84±0.17)),ODw(protein (0.69±0.04);gene (0.88±0.16)) and ODr(protein (0.53±0.07);gene (0.70±0.15)) were increased compared with the ODn(protein (0.31±0.09);gene (0.56±O.16)) group (P＜0.05).(3)The concentration of 5-HT of hippocampus and serum in Dn and On group were decreased contrast to the Sn group (P＜0.05).The concentration of 5-HT of hippocampus and serum in ODs,ODx,ODw and ODr group was increased compared with the ODn group (P＜0.05).Conclusion The antidepressants can up-regulate the expression of THP2 and the concentration of 5-HT in the OVX rats with depressive disorder.Sertralin is stronger however Reboxetine Mesylate is the poorest.

Objective To investigate the expression of extracellular matrix metalloproteinase inducer (CD147), nuclear factor kappa B (NF-κB) in esophageal squamous cell carcinoma tissue and probe their relationship to invasion and metastasis of esophageal carcinoma. Methods Immunochemical staining was performed to detect the expression of CD147 and NF-κB protein in 40 cases of esophageal carcinoma tissues and 40 cases of normal control. Results The positive rate of CD147 and NF-κB in esophageal carcinoma tissues and in adjacent noncancerous tissues were 77.5%/87.5% Vs 25%/42. 5%, P＜0.05.The positive rate of their prophase and metaphase-advanced stage esophageal carcinoma tissues were 33.3%/90.3% Vs 55.6%/96.8%, P＜0.05..The positive rate of non-metastatic and metastatic esophageal carcinoma tissues were 28.6%/87.9% Vs 42.9%/97%, P＜0.05.Conclusions Our results suggested that the increased expression of CD147 and NF-κB contributed to tumor angiogenesis in esophageal squamous cell carcinoma, which might be through the common pathway.

Objective To explore the effect of psychological intervention combined with drug multimodal treatment on analgesic effect and stress reaction in Department of orthopedics.Methods80 cases of Department of orthopedics patients in Ruian City People's Hospital from March 2016 to March 2017 were selected and randomly divided into control group and observation group, 40 cases in each group.The control group was treated with drugs only, and the observation group was treated with psychological intervention combined with multi multimodal.The analgesic effect and stress response of the 2 groups were observed.ResultsThe satisfaction rate in observation group were significantly higher than the control group.There was no significant difference in heart rate, diastolic blood pressure and systolic blood pressure between two groups before intervention, after group intervention, the changs in observation group were relatively small, and the difference was statistically significant(P<0.05).Visual acupuncture scores were observed at different time points,the pain in the observation group was significantly lower than that in the control group, the difference was statistically significant (P<0.05).ConclusionMultimodal therapy for analgesia in the Department of orthopedics and found psychological intervention combined with stress reaction the drug, compared with simple drug intervention, can play a good analgesic effect, at the same time to reduce the patient's stress response, so it is worthy of reference.

Objective:The drug resistance of E.coli to fluoroquinolones is getting stronger in the recent years.This study aimed at the drug resistance of E.coli induced by fluoroquinolones in vitro,so as to provide a guidance for the rational use of antibiotics and effective control of nosocomial infections.Methods: Drug resistance was induced in vitro by a multi-step method in 10 strains of E.coli from clinical isolates with Ciprofloxacin,Levofloxacin and Gatifloxacin.The minimal inhibition concentration(MIC) of the E.coli was determined by the agar dilution method before and after the induction.Results: After in vitro induction,the E.coli acquired a high resistance(MIC ≥ 128 ?g/ml).And one drug could induce different degrees of resistance to the other two.Conclusion: The MIC of the E.coli induced by fluoroquinolones in vitro was increased by 8 to 8 205 folds compared with that before induction,which demonstrated a gradually developed resistance of E.coli strains to fluoroquinolones.

Objective To discuss the preventive and curative effect of Kanghuier transparent dressing on prevention of vascular and local tissure injury induced by continous dripping of 5-fluorouracil.Methods Patients(128 cases)were divided into the observation group and the control group with 64 cases in each group.Cleaning of the local skin around puncture point and use of Kanghuier transparent dressing were adopted in the observation group.The control group receivecl no vascular protective measures.The effect of phlebitis prevention was compared between the two groups.Results Statistical difference existed in the incidence rate of phlebitis between the two groups(P＜0.01),the rate of the observation group was lower than that of the control group.Conclusion Kanghuier transparent dressing can prevent the vascular and local tissure injury caused by chemotherapy for tumor patients.

ve To assess the cytogenetic toxicological safety of fish raised in water containing effluent. Methods The mutagenecity of organic extracts of uncooked meat specimens of fish raised in fishpond water mixed with sewage water taken from South and North Sewer Drainage Canals of Tianjin was detected by mice bone marrow micronucleus test. Results The organic extracts of uncooked fish meat specimens showed positive result for all of the 3 observed groups of fish raised in water of fish ponds with sewage, and negative result for fish raised in clean fishpond water in micronucleus test. Conclusion The results indicated that some kinds of organic mutagenic compounds ex-isted in the uncooked meat of fish raised in fishpond water containing effluent, which might have some potentially hazardous effects on human health through the food chains.

Objective To study the mutagenicity of fish-raising water containing effluent. Methods The niuta-genieities of water samples from waste water canal, the fish-raising water(fish-pond water) treated by presedimenta-tion in oxidation tank were detected using micronucleus test of vicia faba root tip cells. The micronucleus test of peripheral red blood cells was carried out among fish raised in water containing effluent. The water samples and fish collected from reservoir as controls. Results The micronucleus test of vicia faba root tip cells showed that the micronucleus rates in treated fish-pond water and waste water were significantly higher than those of negative control group (distilled water) (P 0.05) . The frequencies of micronucleus in peripheral red blood cells of fish raised in treated fish-pond water were significantly higher than those of fish raised in reservior water (P

In vitro fertilization and embryo transfer (IVF- ET) technology is now widely used in the world in infertility patients. Psychological intervention can help the IVF- ET patients and their spouses reduce the negative emotions and improve pregnancy outcomes. This review provides a summary of the negative emotions in IVF- ET couples and the psychological intervention on them, so as to provide reference to establish a systematic and standardized clinical psychological nursing mode.