Purpose To study immunohistochemical quality of prostatic basal cell(PBC). MethodsTherty-seven of basal cell of benign prostatic hypertrophy (BPH) were studied using routine HE stavningand En Vision immunohistochemical methods. Results The positive rate of 34βE12 in PBC were 100 %;but all of the PBC were negative with Vim and SMA; the positive expression of PsAP PSA in PBC were35% ;expression of ck in the PBC was 71% and the positive rate of S-100 in PBC was onty 13.5%.Conclusions The resuts suggest that high molecular weight keratin 34βE12 is a specific marker to PBC;PBC has ability to adenoid epithelia differentiation of prostate and hyperplasia itself. PBC play an importantrole in growth, hyperplasia and disease of prostate. The PBC unlike myoepithelia, the ability to myoepitheliadifferentiation is to go a step further controversial opinion.

Partial hepatectomy (PH) is an effective method for the treatment of various liver tumors,and liver regeneration dysfunction is one of the most serious complications.Therefore,liver regeneration (LR) after PH is particularly important.In this paper,we reviewed the molecular mechanisms of LR including cellular factors,growth factors,metabolism and related signaling pathways,and reviewed the research progresses of the traditional Chinese medicine (TCM) in promoting liver regeneration.The aim was to further clarify the mechanism of the occurrence and development of LR,and to provide valuable information for the development of drugs for treating liver regeneration disorders.

Objective: To observe the therapeutic effect of nicorandil on microvascular angina pectoris (MAP). Methods: A total of 60 MAP patients were randomly divided into nicorandil group (n=30) and routine treatment group (n=30), and they were treated for 12 weeks. Onset times and duration of angina pectoris, changes of related parameters of ECG treadmill exercise test before and after treatment, and total effective rate were compared between two groups. Results: Compared with before treatment, there were significant reductions in onset times and duration of angina pectoris and maximum depression extent of ST segment (P<0.05 or <0.01) and significant increase in total exercise time (P<0.01) after treatment in both groups; compared with routine treatment group, there were significant reductions in onset times of angina pectoris [(10.3±1.6) times/week vs. (9.6±1.7) times/week] and maximum depression of ST segment [(0.8±0.3)mm vs. (0.6±0.2)mm], and significant increase in total exercise time [(7.8±1.4) min vs. (9.4±1.6) min] and total effective rate (73.3% vs. 93.3%) in nicorandil group, P<0.05 or <0.01. Conclusion: Nicorandil possesses significant therapeutic effect on microvascular angina pectoris, and it is worth further study.

Objective To compare the efficacy of cilostazol and aspirin in the treatment of vascular dementia with white matter lesions.Methods 50 patients with vascular dementia with white matter lesions were randomly divided into control group (aspirin and nimodipine group)and observation group (cilostazol and nimodipine group). The control group was orally given aspirin enteric -coated tablets and nimodipine tablets,the observation group was treated with cilostazol tablets and nimodipine tablets.Then,the patients were followed up,compared the cognitive function and adverse events of the two groups after treatment 6 months and 1 2 months.Results 6 months and 1 2 months after treatment,MMSE score and MoCA score of the control group were significantly improved[the MMSE score and MoCA score before treatment were (20.1 2 ±4.25)points,(1 4.25 ±4.25)points,6 months after treatment were (21 .22 ±4.68)points,(1 6.45 ±3.25)points,1 2 months after treatment were (22.38 ±5.64),(1 6.95 ± 4.68);6 months after treatment,t =0.87,2.06;1 2 months after treatment,t =1 .96,2.1 4,all P <0.05].6 months and 1 2 months after treatment,MMSE score and MoCA score of the observation group were significantly improved than before treatment [before treatment the MMSE score and MoCA score were (1 9.85 ±5.1 4)points,(1 3.98 ± 6.28)points,6 months after treatment were (23.76 ±4.1 5)points,(1 8.75 ±4.28)points,1 2 months after treatment were (25.26 ±3.72)points,(23.95 ±5.43 )points,6 months after treatment t =2.96,3.1 4;1 2 months after treatment,t =4.26,6.00,all P <0.05].6,1 2 months after treatment,the MMSE score between the two groups had significant difference (t =2.03,2.1 3,all P <0.05),MoCA score between two groups had significant difference(t =2.1 4,4.88,all P <0.05).The incidence rates of cerebral hemorrhage in the observation group and control group were 0.00%,1 2.00%,the difference was significant (χ2 =3.1 9,P <0.05).Conclusion Cilostazol and nimodipine in the treatment of vascular dementia with white matter lesions,can significantly improve cognitive function of patients, and has less cerebral hemorrhage,significantly clinical effect,good security.

Objective To analyze perioperative nursing methods of chest wall tumor removal and chest wall reconstruction. Methods 12 patients with chest wall tumor removal and chest wall revascularization were choosed as the research objects,and which were given prospective nursing intervention combined with high quality nursing intervention. Results 12 patients all cured without one case death occurred, and 4 cases complications. Followed up for 6 to 36 months, no local recurrence of alienation, chest wall deformity and rejection. Conclusions Proactive nursing intervention help to predict perioperative complications. High-quality nursing care including psychological nursing, skin nursing, pain nursing, function training etc, can promote patients recovery.

Objective:To observe the emergency treatment effect of levosimendan combined noninvasive mechanical ventilation on acute heart failure during emergency treatment period .Methods :According to random number table , 120 cases with acute heart failure were randomly and equally divided into routine treatment group and levosimendan group (levosimendan + noninvasive mechanical ventilation ) .Improvements of arterial blood gas [partial pressure of carbon dioxide and oxygen (PaCO2 ,PaO2 ) ,pH etc .] after 72h ,change of N terminal pro brain natriuretic pep-tide (NTpro-BNP ) level 4d after hospitalization and mortality rate were observed and compared between two groups .Results:After treatment ,PaO2 ,mean arterial pressure and NTpro-BNP level of the two groups significant-ly improved compared with before treatment ;compared with routine treatment group ,there was significant rise in PaO2 [ (78.2 ± 9.4) mmHg vs .(86.2 ± 10.5) mmHg] and significant reductions in mean arterial pressure [ (86.3 ± 8.2) mmHg vs .(82.2 ± 9.0) mmHg] and NTpro-BNP level [ (4340.5 ± 540.7) pg/ml vs .(4012.1 ± 426.3) pg/ml] in levosimendan group , P<0.01 all;total effective rate of levosimendan group was significantly higher than that of routine treatment group (86.7% vs .76.6% , P=0.047);after three-month follow-up ,mortality rate of le-vosimendan group was significantly lower than that of routine treatment group (11.7% vs .20.0% , P= 0.026 ) . Conclusion:Emergency treatment effect of levosimendan combined noninvasive mechanical ventilation on acute heart failure is significant ,which is worthy of further research and extension .

Objective:To analyze the levels of immune cytokine in peripheral blood(PB) and the expressions of immune related transcription factors in placenta cytotrophoblast,which in patients with pregnancy-induced hypertension(PIH).And to explore the effect of fetal-maternal immune tolerance on pregnancy-induced hypertension.Methods:50 cases patients with PIH were selected as the observation group,and 40 healthy people were chosen as the control group.Enzyme-linked immunoassay(ELISA) was performed to detect the levels of IFN-γ,IL-4,IL-17 and TGF-β in peripheral blood.Quantitative Real-time PCR(RT-qPCR) and Western blot were used to analyze the mRNA expressions and protein levels of T-bet,GATA-3,RORC and Foxp3 in placenta cytotrophoblast,respectively.Results:①Compared with the control group,the IFN-γ and IL-17 levels in peripheral blood in the observation group were obviously higher and the TGF-β level was greatly lower(P<0.05).There was no significant difference of IL-4 level between the two groups(P>0.05),but the ratios of IFN-γ/ IL-4 and IL-17/TGF-β in the observation group were larger than that in the control group(P<0.05).②Compared with the control group,the mRNA expressios and protein levels of T-bet and RORC in the observation group were increased significantly(P<0.05),but that of GATA-3 and Foxp3 were decreased greatly(P<0.05).Thus,the ratios of T-bet/GATA-3 and RORC/Foxp3 in the observation group were significantly larger than that in the control group(P<0.05).Conclusion:PIH is occurred with the imbalance of Th1/Th2 and Th17/Treg,and it might be associated with the mechanism of fetal-maternal immune tolerance disorder that induced by Th cells.

Purpose:To investigate the expression of PCNA、p53、C erbB 2 in colorectal carcinoma and its relationship with host immune reactions. Methods:The expression of PCNA、p53、C erbB 2 was detected by Envision immunohistochemical method in 42 colorectal carcinoma, 35 pericarcinomatous and 10 normal coloerctal mucosa respectively. Host immune reactions (pericarcinomatous fibrosis, mononuclear infiltration and lymph nodes reaction) were also observed. Results:(1) The positive expression of PCNA、p53、C erbB 2 in colorectal carcinoma was significantly higher than in corresponding pericarcinomatous mucosa and normal colorectal mucosa respectively( P 0.05). (3) GH was more evident than PH in colorectal carcinoma and GH had reverse relationship with lymph node metastasis( P

Objective:To investigate the status of shift nurses′ circadian rhythm, sleep quality and job burnout in 3A-level hospitals, and analyze the influence of circadian rhythm and sleep quality on job burnout, so as to provide theoretical basis for reducing the level of job burnout of shift nurses.Methods:A total of 491 shift nurses were investigated with General Information Questionnaire, Circadian Type Questionnaire(CTI-11), Pittsburgh Sleep Quality Index (PSQI) and Maslach Burnout Inventory General Survey(MBI-GS).Results:The Flexibility or Rigidity score of shift nurses was 10.64±4.14, the Languid or Vigorous score was 17.67±4.80, the PSQI score was 7.47±3.66, the MBI-GS score was 51.14±15.11. The results of multiple regression analysis showed that flexibility or rigidity, languid or vigorous, sleep quality were the influencing factors of emotional exhaustion( t value was 7.415, - 5.281, 7.153, P<0.01); flexibility or rigidity, languid or vigorous, sleep quality were the influencing factors of depersonalization ( t value was 4.828, - 4.079, 4.959, P<0.01); flexibility or rigidity was the influencing factors of professional efficacy( t value was - 3.887, P<0.01). Conclusions:The job burnout of shift nurses in Tianjin 3A-level hospitals were serious. The circadian rhythm and sleep quality are the important factors that affect the job burnout of shift nurses. According to the type of circadian rhythm and sleep quality of nurses, nursing managers can take personalized measures to reduce their level of job burnout.

The liver is one of the most important organs in human body, which participates in metabolism and detoxification. The liver is easily affected by various drugs and their metabolites. Drug-induced liver injury, also known as drug-induced liver disease, refers to the liver damage or allergic reaction to liver caused by drugs and their metabolites. MicroRNA (miRNA) is a kind of small non-coding RNA, which plays an important regulatory role in many diseases. In this review, the effects and mechanisms of miRNAs in drug-induced liver injury were summarized.