The volatile oils from the crude drug of Panax notoginseng (Burk.) F. H. Chen (San-Qi) being on the market were analyzed by Ge/Ms/Ds and standard mass-spectrogram atlas. 34 kinds of components were identified and their relative contents were determined. Among them 13 molecules of sesquiterpene and 2 of sesquiterpenol appeared.

The volatile oils from Panax Japonicus C. A. Meyer var major (Burk) Wu et Feng grown in Teng-chong County were analyzed by GC/MS/DS. 27 kinds of components were identified and their relative contents were determined. Among them 10 molecules of sesquiterpene and 1 of sesquiterpenol appeared.

The Volatile Oils from panax Quinquefolius grown in Li-jiang county, Yunnan, China were analysed by GC/MS/DS and standard mass-spectrogram atlas. 26 Kinds of compounds were identified and their relative contents were determined.The Volatile oils from Li-jiang panax Quinquefolius are proved to be significantly different in composition and content from those panax quinquefoliuses imported form abroad or grown in other regions in China.

Objective To investigate the resistance of multi-drug resistant Acinetobacter baumannii against aminoglycosides and its resistance genes.Methods The minimal inhibitory concentrations (MICs) of seven antimicrobials (gentamicin, amikacin, tobramycine, netilmicin, neomycin, streptomycin and kanamycin) against 20 strains of multi-drug resistant Acinetobacter baumannii were detected by agar dilution method. Meanwhile, two resistance genes of aminoglycoside nucleotidyltransferase were amplified by PCR and vertified by DNA sequencer.Results It was found that MIC50 and MIC90 of gentamicin, amikacin, streptomycin and kanamycin in 20 strains of Acinetobacter baumannii were all above 1 024 mg/L, and their resistant rates were 95%, 95%, 100% and 90% respectively, while the resistant rates of tobramycin, netilmicin and neomycin were 85%, 90% and 40% respectively. Two aminoglycoside-modification genes ant(2″)-Ⅰ and ant(3″)-Ⅰ were detected in 20 strains, with 55% and 80% of positive rate respectively. The double positive rate of two resistance genes was 50%.Conclusion The resistance of multi-drug resistant Acinetobacter baumannii against aminoglycosides was closely associated with ant(2″)-Ⅰ and ant(3″)-Ⅰ genes.

Objective] To observe the clinical effect of Yiyuan Shengjing Decoction on similar climacteric syndrome of breast cancer after treated with tamoxifen. [Method] Choose 60 cases of breast cancer, randomly divide them into control group 30 cases and treatment group 30 cases, the control one were treated with tamoxifen and oryzanol, the treatment one, with tamoxifen and Yiyuan Shengjing Decoction. After 4w, evaluate the efficacy, compare their clinical symptoms score, hormone levels and endometrium thickness, and calculate the clinical relieving rate. [Result] After treatment, both groups clinical symptoms were relieved more than before treatment, with difference of statistical meaning; comparing on clinical symptoms scores, the treatment was much less than control one, with statistical meaning; on clinical relieving rates, the treatment group reached 83.33%, while 26.67% for control one, both had difference of statistical meaning. The comparison of reproductive hormone levels and endometrium thickness, the difference had no statistical meaning. [Conclusion] Yiyuan Shengjing Decoction treating breast cancer similar climacteric syndrome after treated with tamoxifen had marked cure effect, without influencing reproductive hormone level and endometrium thickness.

Objective To explore the effect of family doctor contracting service combined with drug therapy on the quality of life of patients with hypertension. Methods Selected in May 2016 to May 2017 in a city in a district of three community health service center clinics diagnosed with hypertension in patients with 840 cases, were given amlodipine besylate tablets treatment, and the implementation of conventional health management And community intervention. According to whether or not to accept the family doctor contract service is divided into observation group (a total of 435 cases) and the control group (a total of 405 cases). Health status questionnaire (SF-36) was used to assess the quality of life before and after the intervention, and the blood pressure control and quality of life improvement were compared between the two groups before and after treatment.Results There was no significant difference in the blood pressure level between the two groups before treatment. The systolic and diastolic blood pressure in the observation group were better than those in the control group (P<0.05). There were no significant differences in physiological function, physiological function, physical pain, general health, mental health, energy, social function, affective function and total score of SF-36 before treatment. The scores of the observation group were significantly higher than those of the control group before and after treatment. The difference was statistically significant (P<0.05). Conclusion Family doctor contract service combined with drug therapy can effectively control the patient's blood pressure levels, improve the quality of life.

Hypertension is a kind of chronic disease effecting many population, and its prevalence has a rising trend with the age. It keeps in a increasing trend in chinese population, and follows high rates of prevalence, mortality, morbidity and three low rates of wareness, treatment, control. Hypertension is caused by a number of factors, and its incidence is closely related with unhealthy diets. Its incidence may be decreasing gradually by changing lifestyles, taking healthy and reasonable diet. To provide some basic data of healthy lifestyles and diet for people, describe and summarize the relation of hypertension and diets in this article.

Objective To investigate the retinoic acid incubation effect on proliferation of U251 cell line and effect on MAPK signal pathway. Methods ATRA solution of different concentration on the U25 1 glioma cells were incubated,the influence of ATRA on the proliferation of U25 1 cells were detected,and the proteins of MKPs and MAPK signaling pathways were detected by qRT-PCR and Western blot.Using Graph Prism 5 software for quantitative analysis of experimental results.Results Compared with control group,ATRA could effectively inhibit the proliferation of U25 1 glioma cells, in a concentration dependent manner.QRT-PCR results showed that,different concentrations of ATRA after incubation for 48 hours,the expression of MKPs mRNA changed,but the changes of MKP-5 and expression of 67LR was different,explained the main differences between the two methods of the MAPK signaling pathway was the regulation of MKP-5.Western blot results showed that the ATRA,after 48 hours of incubation,the protein MAPK pathway had changed in phosphorylation, which showed that ATRA protein in the MAPK signaling pathway through control of the degree of phosphorylation on U25 1 cell line regulation.Conclusion Retinoic acid and retinoic acid receptor play its physiological effects and regulate human glioma cell line U25 1 proliferation through different combination.Retinoic acid could not only reduce the expression of phosphorylated ERK1/2 to inhibit tumor proliferation,but also regulate three kinds of protein phosphorylation,therefore its mechanism will be more complex,at the same time that the MAPK signaling pathway plays a crucial role in tumor proliferation process.

Background EyeLink 2000 high speed video eye tracker has been used in ophthalmology to evaluate the ocular movement ability.Its systemic precision depends on the performance of the eye tracker, and it is also an essential parameter for the evaluation of the data quality.Therefore, the impact of infrared intensity should be illustrated.Objective This study was to assess the systemic precision of the video eye tracker and evaluate the impact of infrared intensity on the result.Methods The EyeLink 2000 high speed video eye tracker and a pair of artificial eyes were used in this study.Firstly the artificial eyes were fixed to the corresponding position as the real human eyes.Each session of recording was undertaken under the different infrared intensities (100％ , 75％ and 50％).The sampling frequency of binocular recording was set as 1 000 Hz.Each session was sequentially recorded for 60 seconds.The systemic precision was calculated as the root mean square (RMS) of angular distances between successive data points.The RMS values under the different infrared intensities were compared.Results The highest RMS values were (0.005 01 ± 0.000 20) ° under the 100％ infrared intensity.As the decline of infrared intensities ,RMS values were gradually raised, showing a significant difference among different infrared intensity groups (F =3 791.447, P＜0.01).The differences were also significant between different groups (all at P＜0.05).Conclusions EyeLink 2 000 video eye tracker has good system precision.The infrared intensity influence the system precision of the video eye tracker.

Objective To summarize the clinical experience of minimally invasive treatment (sclerosing therapy, intravascular intervention, laser coagulation, etc) for Klippel-Trenaunay syndrome (K-TS). Methods A total of 32 patients with K-TS were treated in this hospital from February 1989 to November 2004. Vascular embolization was used in patients with abnormal arteriovenous fistula or abnormal collateral arterial pathway. The insufficient valves of the deep veins were minimally invasively repaired. Laser coagulation was utilized for treating bulky varicosities. For angiomas and engorged venous plexus of the limbs, the sclerosing agent was injected. Results Varicosis, including reticular venous dilation, subsided completely. Angiography revealed an immediate disappearance of arteriovenous fistula and abnormal blood supply of the femur. The enlargement of involved limbs was diminished gradually. The angioma became completely sclerous, disappeared or decreased in size, without dwindling under pressure. In patients with venous valve reconstruction, Doppler ultrasonography showed no reflux. Follow-up for 1~7 years (mean, years) in all the 32 patients found no recurrence. Conclusions Minimally invasive treatment, including intravascular intervention, laser coagulation, sclerosing agent injection, mini-incision valve repair and so on, is effective for the management of Klippel-Trenaunay syndrome.