Objective To analyze feasibility and safety of sedation with midazolam combined with fentanyl and propofol in patients undergoing gastrointestinal endoscopy,and provide reference for clinical anesthesia.Methods 120 cases were selected with gastrointestinal endoscopy, then divided into observation group and control group randomly, 60 cases in each group, the observation group was anaesthetized by midazolam midazolam combined with fentanyl and propofol, the control group was anaesthetized by fentanyl combined with propofol, the change of heart rate, mean arterial pressure and oxygen saturation in different time between the observation group and the control group were compared, the induction time, the examining time, the recovery time and the time of leaving hospital, the incidence of hypotension, cardiac tachycardia, hypoxia and the incidence of adverse reactions were compared.Results The heart rate, mean arterial blood pressure and blood oxygen saturation decreased to the lowest level in 2 groups at T1.There was no significant difference in mean arterial pressure, heart rate and blood oxygen saturation between the observation group and the control group.The induction time in the observation group was shorter than the control group, and the leaving hospital time was longer than the control group(P<0.05).There was no significant difference in the incidence of hypotension and tachycardia between the two groups of patients.The incidence of adverse reactions in observation group was lower than that in control group, the difference was statistically significant(P<0.05).Conclusion In gastrointestinal endoscopy, midazolam combined with fentanyl and propofol is feasible and safe, because of steady hemodynamics, faster induction without prolonged recovery, reduce the incidence of adverse reactions.

Objective:To measure the physiological response and subjective experience differences between feminine and masculine women inducing anger,exploring women's susceptibility to depression.Methods:Thirty feminine women and 26 masculine women were selected from 745 college women students who filled in the Chinese College Students'Sex Role Inventory-50 items (CSRI-50),according to median classification method.Physiological response,including heart rate,skin conduct,respiration rate were recorded using Biotrace software from 5-10 min before watching films to films end,state anger were measured before and after films by the State Anger Scale(SAS),one of the State-Trait Anger Expression Inventory-Ⅱ (STAXI-Ⅱ).Results:With inducing anger,the skin conducts were lower in feminine women than in masculine women[(6.1 ±4.6) μmho vs.(9.4 ±6.0)μmho,P ＜0.05],while the respiration rates were higher in feminine women than in masculine women[(19.4 ±2.8) bpm vs.(17.7 ± 3.3) bpm,P ＜ 0.05].The SAS sores were higher in feminine women than in masculine women[(31.6 ± 8.8) vs.(26.5 ± 9.2),P ＜ 0.05].Conclusion:It suggests that feminine women subjectively report more anger and physiological responses may be greater in watching anger inducing films,mainly in RSP rate,however masculine women's SC is higher,which indicates that future researches of anger and depression should consider sex role.

Objective To evaluate the difference of myocardium strain between myocadial infarction and ventricular aneurysm (VA) by two-dimensional speckle-tracking imaging in rabbits.Methods 26 healthy New Zealand white rabbits were enrolled in this study,6 were put into the control group and 20 in the experimental group.models of myocardial infarction concomitant with VA were prepared by the ligation of the middle segment of left anterior descending artery and left circumflex artery.At 4 weeks after the procedure,according to the pathological inspect and echocardiography for whether VA formed,the experimental animals were divided into the VA group and the myocadial infarction group.Echocardiography was performed to measure the LV cavity size and cardiac function.Radial strain rate (SrR) and circumferential strain rate(SrC) indexes were measured by 2D-STI software for each animal at the level of apex in left ventricular short-axis view,and then calculated the strain rate decrease percentage in each segment.Results 1)Compared with the control group,SrR-S,SrR-E,SrR A,SrC-S,SrC-E,SrC-A in all segments were reduced significantly in the VA group and the myocadial infarction group (P ＜ 0.05),the VA group was the most significant.2)Compared with the myocadial infarction group,the strain rate decrease percentage of SrR-S,SrR-E,SrR-A,SrC-S,SrC-E,SrC-A in all segment had significant in the VA group.Comparison between the each section,the strain rate decrease percentage of SrR-Santerior wall,SrR-Sateral wall,SrC-Santerior wall,SrC-Slateral wall were the most significant.3)There were the tighter relationship between the strain rate decrease percentage of SrR-Santerior wall,SrC-Santerior wall,SrR-Slateral wall,SrC-Slateral wall and left ventricular ejection fraction (r =0.82,0.72,0.75,0.71,repectively,all P ＜ 0.05).4) The ROC curve analysis showed that when the strain rate decrease percentage was 60％ as the critical point,the strain rate decrease percentage of SrC-Santerior wall,SrC-Slateral wall,SrR-Santerior wall,SrR-Slateral wall had a sensitivity of 83.30 ％,84.40％,83.30％,67.70％％ and a specificity was 73.60％,78.20％％,69.70％,83.30％ for prognosis ventricular aneurysm,repectively.Conclusions After ventricular aneurysm formation,anterior and lateral wall myocardial strain were give priority to the myocardial mechanics reconstruction,thus systolic myocardial strain rate decrease percentage can be important indicator of infarction myocardial developing into ventricular aneurysm.

Objective To explore the application of 2010 edition cardiopulmonary resuscitation clinical nursing teaching pathway combined with OSCE mode in teaching and assessment,and improve clinical emergency response capabilities.Methods 99 target school undergraduate nursing students were selected as research objects,and were randomly divided into the traditional group (50 students) and the combination group(49 students).The traditional group adopted the traditional CPR teaching and assessment methods,the combination group took the 2010 version of CPR pathway and OSCE clinical nursing teaching mo de.The mastery of CPR was compared between two groups.Results The mastery of cardiopulmonary resuscitation skills of the combination group was significantly better than that of the traditional group.Conclusions Implementation of the combination of 2010 version of the CPR and the OSCE clinical nursing teaching pathway mode can effectively promote the mastery of CPR skills by students,effectively improve the first-aid skills and adaptability of students.

OBJECTIVE:To observe the efficacy and safety of aerosol inhalation recombinant human interferon α1b in the treat-ment of bronchiolitis in children. METHODS:60 children with bronchiolitis were randomly divided into low-dose group,high-dose group and control group. All children were given tracheal suctioning,phlegm dispersing and other symptomatic treatment. Based on it,low-dose group was given recombinant human interferon α1b 1-2 μg/(kg·times),adding into 3 ml 0.9% Sodium chloride injec-tion,compression aerosol inhalation,twice a day;high-dose group was given recombinant human interferon α1b 3-4 μg/(kg·times), adding into 3 ml 0.9% Sodium chloride injection,compression aerosol inhalation,twice a day;control group was given ribavirin 10-15 mg/(kg·d),adding into 5% Glucose injection at ratio of 1∶1 by intravenous infusion,once a day. The treatment course for all groups was 5-7 d. Clinical efficacy,disappearance time of cough,respite,rale and three depressions,hospitalization time and incidence of adverse reactions in all groups were observed. RESULTS:Disappearance time of cough,respite,rale and three depres-sions and hospitalization time in high-dose group were significantly shorter than low-dose group and low-dose group shorter than control group,the differences were statistically significant(P<0.05). Total effective rate in high-dose group was significantly high-er than low-dose group and low-dose group higher than control group,the differences were statistically significant (P<0.05). There were no obvious adverse reactions during treatment. CONCLUSIONS:Based on conventional treatment,both efficacy and safety of aerosol inhalation recombinant human interferonα1b in the treatment of bronchiolitis in children are good.

Since the first paper reported the finding of genetic variation contributing to human age-related macular de?generation by genome-wide association study (GWAS) in 2005, large number of human complex diseases associated genetic variants have been identified through GWAS method. However, the biological function of these genetic variants is not very clear. The present paper reviewed the methods of fine-mapping and the progress of the functional studies for these suscepti?bility variants. In the post GWAS Era, the study of genetic mechanisms can help us to understand the disease pathogenesis.

Objective To study the correlation between psychological state and coping styles of primary caregivers of stroke patients.Method 286 primary caregivers were screened out by the random sampling method for the investigation of their psychological states and coping styles using SCL-90 and the medical coping modes questionnaire(MCMQ).Results There were significant differences between primary caregivers and Chinese norm in terms of all factors(P<0.01).The major caregivers with higher education were scored significantly lower than those with less education on the factors of depression,anxiety,hostility,fear,psychoticism (P<0.01).There were significant different differences between primary caregivers and Chinese norm in coping style(P<0.01): the score on confrontation in the coping style lower than the norm while the score on avoidance in the coping style higher than in norm.The confrontation and avoidance in the primary caregivers were negatively correlated to the factors of SCL-90(P<0.05)and yielding in the coping style positively to the factors of SCL-90(P<0.05).Conclusions The primary caregivers of stroke patients are at the poor psychological states and they tend to take the passive and negative coping styles.The more they take active coping style,the better their mental states grow and vise versa.Therefore,the caregivers need professional training so that they can use the supports from their families and be encouraged to speak out their poor moods.Post-discharge visits to them,besides,are helpful for the improved psychological states for the improvement of their quality of life.

Objective To investigate the role of Twist and vascular endothelial growth factor (VEGF)in the development of epithelial ovarian cancer and their relationship with clinical pathological features.Methods The expressions of Twist and VEGF were detected by immunohistochemical staining (SP)method in 80 cases of epithelial ovarian cancer and 10 paired cases of normal ovarian tissue.Results In the 80 cases of ovarian cancer tissues,the positive expression rate of Twist and VEGF in epithelial ovarian cancer was 85% and 86.25%,respectively,which were remarkably higher than those in normal ovarian tissues (P <0.01).The expressions of Twist and VEGF were closely associated with clinical stages,pathological grades and lymph node metastasis,but not correlated with age or pathological patterns (P >0.05).There was a significant correlation between the expressions of Twist and VEGF in epithelial ovarian cancer (r =0.646,P <0.01).Conclusion The high expressions of Twist and VEGF may play a potential role in the occurrence,development and invasion process of epithelial ovarian cancer.

Objective To explore the influence of the medication adherence and family function on mental disability status of the schizophrenia patients.Methods Morisky Medication Adherence Questionnaire(MAQ),Assessment Schedule Ⅱ (WHO-DAS Ⅱ),Family APGAR Index(APGAR) were used to analyze the influence of the medication adherence and family function on the schizophrenia mental disability patients.120 convalescence mental disability patients were included in Inner Mongolia Anding hospital.Results The mean score of medication adherence and family function of the schizophrenia mental disability patients was (2.40±1.10) points and (4.87±2.42) points,they both played a significant role on the effect of mental disability.The main effect of medication adherence and family function were significantly different.Through the simple effect analysis,the mental disability score was different in the various medication adherence level under the various family function situation,and the mental disability score was also different in the various family function situation under the medication adherence level.Conclusions Both medication adherence and family function have effect on mental disability status of the schizophrenia patients.The medical staff should take targeted measures to delay or improve patients' mental disability status.

Objective To know the relationship between loneliness and the quality of life of urban and rural absent parents in the Inner Mongolia Autonomous Region,clear the influence factors of loneliness in the absent parents.Methods 441 cases of urban and rural absent parents were selected from the Chifeng district of the Inner Mongolia Autonomous Region were valuated with the general information questionnaire,UCLA Loneliness Scale and MOS 36 item short form health survey (SF-36).Results Rural cases had a higher level of loneliness when compared with the urban cases,t=4.60,P＜0.01.There were significant differences in the general score and the scores of each dimension between the two groups,P＜0.05.Loneliness score was significantly negative correlation with the quality of life in all dimensions,P＜0.01.Regression analysis showed that health,marital status,income and the education level were the main influence factor of loneliness in the urban and rural absent parents.Conclusions Compared with the urban absent parents,rural cases had more obvious loneliness and lower quality of life.Loneliness levels influence the quality of life of absent parents.Loneliness condition of urban and rural absent parents were influenced by various factors,the cases with characteristic of frail,single,low education level and poor economic condition should be the focus group to intervene.