Objective To investigate the free radical scavenging effects and antioxidation activity of squid(Dosidicus eschrichitii Steenstrup) skin gelatin hydrolysate.Methods The free radical scavenging effects of the hydrolysate on superoxide and hydroxyl free radicals were assessed by chemiluminescence analysis method.The activity of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px) and content of malondialdehyde(MDA),hydroxyprolyne(Hyp) in blood serum and skin tissue in galactose treated mice were analyzed after 42 days of hydrolysate feeding.Results and Conclusion The fraction with relative molecule weight less than 2000 D of the hydrolysate had the highest free radical scavenging effects on superoxide and hydroxyl free radicals,and the activities of SOD,GSH-Px were enhanced,the quantity of MDA in blood serum and skin tissue of the mice was decreased and the quantity of Hyp in skin tissue of mice was increased after the hydrolysate administration.

Guidelines as Topic ; Hand-Arm Vibration Syndrome ; diagnosis ; Humans ; Occupational Diseases ; diagnosis ; Reference Standards

Nursing is an important component in the clinic of traditional Chinese medicine,therefore having been emphasized broadly and continuously by generations of doctors.However,because of the combination of the treatment and nursing in traditional Chinese medicine,nursing hash't formed its own system until now.Literatures on the knowledge of nursing in traditional Chinese medicine are also sporadically recorded.The author focused on the development of nursing in traditional Chinese medicine to demonstrate that nursing in China has a long-term history with plenty of contents and various techniques.

Web Quest teaching is an effective model for teaching reform in the means explorative kinesiology learning, which contributes to the abilities of discovery, autonomous, inquiry and cooperative learning.

This article reviewed the stress, coping to the stress, the common mental troubles and the intervenient strategies of the parents with cerebral palsy children.

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Mass higher education" is necessarily for all countries, and the characters of "Mass higher education" ofChina decides that the Chinese government must assume corresponding duty. To provide proper educational outlays isthe chief and essential duty of the government. So if the problem is not solved or is inconsequently solved,the course of"Mass higher education"in China will be baffled and the reputation of the gouvernment will be affected as well.

OBJECTIVE To investigate ?-lactamases genes in Pseudomonas aeruginosa(PAE) isolated from clinical patients.METHODS The drug-sensitivity to 16 antibiotics was detected by the disc agar diffusion(DAD) and microdilution.The related drug-resistance genes TEM,SHV,OXA-10,PER,VEB,GES,CARB,IMP,VIM,SPM,GIM,DHA,F-OX,MOX,CTX-M9,CTX-M1 and OprD2 were detected by PCR method.RESULTS Resistant rates of 34 MRPA to amikacin,ceftazidime,ciprofloxacin,cefoperazone/sulbactam,cefepime,imipenem,levofloxacin,piperacillin/tazobactam,aztreonam,piperacillin and tobramycin were 14.7%,29.4%,61.8%,67.6%,70.6%,73.5%,76.5%,76.5%,79.4%,91.2% and 94.1%,respectively,with 100% to others antibiotics.The positive rates of gene encoding extended-spectrum ?-lactamases FOX,IMP,DHA,and VIM were 23.5%,11.8%,11.8% and 5.9%.Twenty-four were deleted out outer membrane protein gene oprD2.Other ?-lactamase genes were absent in all isolates.CONCLUSIONS Clinical P.aeruginosa carries various ?-lactamases genes,with high rate of oprD2 gene.

OBJECTIVE:To develop an HPLC method for determination of main component in low concentration of alprostadil for injection. METHODS: Diode array detector was used for full wavelength scanning of the low concentration of alprostadil sample solution (20 ?g?mL-1) with detection wavelength set at 214 nm and the reference wavelength at 250 nm. The mobile phase consisted of acetonitrile-0.02 mol?L-1 KH2PO4 solution (pH 4.9,40∶60) at a flow rate of 1 mL?min-1. RESULTS: The linear range of alprostadil was 10～320 ?g?mL-1 (r=0.999 9) and the average recovery rate was 98.26% (RSD=1.68%). CONCLUSION: The method is of low cost and it is applicable for the determination of the low concentration of alprostadil for injection.

The article is to illustrate the application of teaching design,multi-media,network teaching and long-distance education,and the introduction of virtual reality techniques in medical teaching?