Objective In order to provide a large quantity of retinal pigment epithelial cells (RPE cells) in vitro, we want to estab-lish method for culturing pig RPE cells. Methods RPE cells were separated with trypsin and cultured in vitro. The cells were identified by immunohistochemical staining with anti-human keratin and transmission electron microscope (TEM). Results Cultured RPE cells gradually presented transparent and fusiform shape. Immunohistochemical staining demonstrated that the cells were stained by anti-human keratin, and the cultured cells showed typical ultrastructure of RPE cells. Conclusion The cultured cells may be the foundation of pig RPE cells.

OBJECTIVE: To validate the method for microbial limit test of Niuhuang jiedu tablet.METHODS: The recovery rates of 5 kinds of test organisms including E.coli treated by Niuhuang jiedu tablets were determined by routine method,culture medium dilution method,centrifugation and membrane-filter method,respectively.RESULTS: The centrifugation and membrane-filter method was suitable for determination of the bacterial number of Niuhuang jiedu tablets,while routine method was applicable for determination of bacterial numbers of mold and yeast and test of control bacteria in the microbial limit test.CONCLUSION: The contaminated bacteria in Niuhuang jiedu tablet can be detected accurately by the method established in this study.

Catheter-Related Infections ; drug therapy ; prevention & control ; Catheters, Indwelling ; adverse effects ; Humans ; Intensive Care Units, Neonatal ; Thrombosis ; prevention & control

Anti-Arrhythmia Agents ; therapeutic use ; Arrhythmias, Cardiac ; drug therapy ; Child ; Humans ; Sotalol ; therapeutic use

Leukocytes from peripheral blood of 70 normal Chinese,40 males and 30 females, were cultured in vitro according to a modification of Moorhead's method.Chromosome studies were carried out.Table I lists the results obtained including age group,metaphase plates counted and analysed and the chromosome number of each cell.92.4 per cent of 8031 counted cells had the diploid number(2n=46).Chromosome measurements were made from camera lucida drawings.Measurements were estimated to the nearest 0.1 mm on a fine metric scale.The relative length,arm ratio and centromere index of each chromosome were calculated from measurements of 18 haploid chromosome sets of 11 metaphase plates in 6 males and 4 females.The averages,range of variation and 99% confidence limits are listed in Table Ⅲ.Systematic studies of individual chromosomes under the light microscope revealed the following morphological characteristics.The short arms of all 10 acrocentric chromosomes from groups 13—15 and 21—22 could be shown to possess satellites,but in general only 2—8 of these chromosomes at a time carried satellites.In many cells the satellite-carrying chromosomes tended to associate with each other through their satellites.The number of chromosomes involved in each association varied between 2 to 5,occasionally 6.It was not rare to find secondary constrictions to occur regularly in certain regions of chromosomes 1,4,9,13,and 16.Attachments of satellites to such regions of chromosomes 1,4,9,and 13 were also observed.Karyotypic analysis of 357 metaphase plates led us to conclude that in most cases the unequivocal identification of chromosome pairs 4—5,6—12+X,13—15,19—20,and 21—22 was im- possible.Accurate characterisation of these chromosome pairs will have to await further technical advances. The length of the Y chromosomes was systematically measured in order to deter- mine its variability and heritability.Among our sample of 40 male subjects 29 had a Y chromosome slightly larger than chromosome 21,8 slightly smaller or larger than chromo- some 18(long Y chromosome),and 3 smaller than chromosome 21(short Y chromo- some).The heritability of the size of the Y chromosome was established(Fig.Ⅳ). “Spontaneous”aberrations were discovered in 34 of 70 cases.Aberration rates varied from 0.88 to 13 per cent.The relation between aberrations and radiation injuries was briefly discussed.

Objective Analysis of K.pneumoniae infection in the Pediatrics and the Neonatology clinical characteristics of the resistance,that can guide and use medicine clinically rationally.Methods The sensitiveness test and ESBL detection to the 20 kinds of antibiotics were examined by Automatic Instrument bacterial identification and susceptibility testing ;ESBL positive strains with the standards disk diffusion method phenotypic confirmatory test.Results The 207 strains of K.pneumoniae from fecal specimens were 77.3% and from respiratory specimens were 18.8%.Imipenem and meropenem sensitivity 100%,followed by compound piperacillin,ciprofloxacin,compound amoxicillin and cefoxitin,that sensitive rates were 98%,97%,96% and 96%,;ceftazidime,cefepime,cefotaxime lower sensitivity were 75%,75%,78%.50 ESBL-producing strains,accounting for 24.2%.Conclusions K.pneumoniae have different levels of the drug resistance expcept Imipenem and meropenem,and the multi-drug resistant strains ratio and the positive rate are high.The sensitiveness of penicillin compound containing enzyme inhibitor is second only to the two described above,and they are economy and effective.Considering by medicine economics,the kinds of medicine is preferred on according to the drug sensitivity.

Objective To comprehend the etiology and the clinical characteristics of hypereosinophilia.Methods The clinical data of 53 patients with hypereosinophilia admitted to Peking Union Medical College Hospital from November 1992 to February 2005 were analyzed.Results The causes of reactive hypereosinophilia included allergic diseases,infections,connective tissue diseases,malignant diseases,lung diseases,eosinophilic gastroenteritis.idiopathic hypereosinophilic syndrome had longer course of disease;more organ involved than reactive causes.The incidence of lymph node enlargement,spleen enlargement and thrombus was higher than the reactive.IHES had higher level of white blood cell count,absolute eosinophilic count,IgG and CRP.Conclusion The etiology and clinical characteristics of hypereosinophilia are various,so the differential diagnosis of hypereosinophilia is very important.

OBJECTIVE:To provide references for the improvement of civil relief system for adverse drug reactions.METHODS:The approaches for Japan in handling the "quinoform" drug injury event were compared with the way of China in dealing with drug injury events.RESULTS & CONCLUSION:The approaches for Japan in handling the"quinoform" drug injury event give some hints for China,i.e.the defective drugs should be comprehensively judged by judges,the causal relations should be determined by using the probability theory and the time point of producers' exemption should be revised.

15℃ . Conclusion The changes of density of different strains of Escherichia coli in the undernourished water samples closely associated with the strain ' characteristics, environment temperatures and preserving time, showed significantly differences.

AIM: To evaluate the value of urethra perfusion using 0.5% povidone iodine liquid (PVP-I) in treatment of patients with chronic urethritis and prostatitis. METHODS: Outpatients with chronic urethritis and prostatitis were assigned to two groups randomly. 38 cases in treatment group were received urethra perfusion with 0.5 % PVP-I liquid plus minomycin, po, for two weeks. 42 cases in control group were received minomycin only. RESULTS: The rates of cure and total efficacy were better in treatment group than in control group (P