Objective To study the relationship between the therapeutic principle and prescription of traditional Chinese medicine and the experimental pharmacologic effects in relation to the treatment of eczema. Methods Murine DNFB allergic contact dermatitis was used as an animal model, and was treated with 4 traditional Chinese medicinal compounds which were composed of Chinese materia medica with activities to suppress type Ⅳ allergic reaction. Results The results showed that the effect of compound Ⅲ composed of Paeoniae obovata Maxim(赤芍), Fructus Gardeniae(栀子), Herba Schizonepetae(荆芥), Herba Spirodelae(浮萍) and Poria(茯苓), possessing multiple potencies of cooling and promoting blood, clearing heat, expelling wind and eliminating dampness, was strongest among the compounds tested, and the compound up regulated the serum level of calcitonin gene related peptide which was lowered in the mice with DNFB induced dermatitis. Conclusion It is suggested that the compounds with suppressive effect on type Ⅳ allergic reaction might improve the therapeutic effect in clinical practice.

Objective To assess the outcomes,complications,and quality of life following endoscopic thoracic sympathectomy(ETS) of the T2~T4 ganglia for treating primary palmar hyperhidrosis(PH). Methods A total of 200 cases were followed with clinic checkups or telephone inquiries for a period of 1~28 months(mean,18.4 months). Results Of the 200 cases,8 cases were loss of follow-up and 192 cases obtained a complete and fully satisfactory alleviation of palmer and axillary hyperhidrosis.No recurrence and severe complications were found.The prime complication was compensatory hyperhidrosis(52/192,27.1%),whose incidence was slightly higher in female and severe patients and unrelated to age.Other complications included 1 case of pneumothorax and 1 case of pulmonary atelectasis. Conclusions Endoscopic thoracic sympathectomy of the T_2~T_4 ganglia for palmar hyperhidrosis is effective,safe and reliable.The procedure improves patients' quality of life and gives satisfactory outcomes.However,all patients should be informed of the common complications,particularly compensatory hyperhidrosis,before the surgery.

Objective To summarize the experience of 200 cases of endoscopic thoracic sympathectomy(ETS). Methods Clinical materials of T_2~T_4 sympathectomy under thoracoscope for treating palmar hyperhidrosis from January 2003 to April 2005 were analysed. Results [WTBZ] All the 200 cases of operation were successfully completed.Symptoms of hyperhidrosis vanished with both hands dry and warm in all the patients.Plamar temperature increased by a mean of(3.1?0.9)℃ after operation.Follow-up observations in 192 cases for 1~28 months(mean,18.4 months) found no recurrence.Compensatory hyperhidrosis was found in 52 cases(27.1%). Conclusions Endoscopic thoracic sympathectomy is a safe,effective,and micro-invasive treatment for palmar hyperhidrosis.

Objective To summarize the experience of perioperative management of lung volume reduction surgery(LVRS) under video-assisted thoracoscopy.Methods A total of 40 patients with severe chronic obstructive pulmonary disease(COPD) underwent LVRS under video-assisted thoracoscopy.A mini-incision thoracotomy was given in 23 of them.During the operation,the Endo-GIA was utilized to resect 20%~30% of total volume of affected lung.Results The LVRS was performed in bilateral lungs in 13 patients(under thoracoscopy only in 7 patients and with the assistance of mini-incision thoracotomy in 6 patients) and in unilateral lung in 27 patients(under thoracoscopy only in 10 patients and with the assistance of mini-incision thoracotomy in 17 patients).There were no surgery-related deaths.All the patients were discharged from the hospital 9~41 days(mean,18 days) after operation.Not only symptoms of dyspnea were obviously relieved but also the respiratory index was upgraded by 1~2 grades.The comparisons on pulmonary functions between preoperative time and 1 month after operation indicated a great improvement postoperatively,including the forced expiratory volume in 1 second(FEV_1)(48.3%?4.9% vs 68.5%?5.6% predicted;t=17.169,P=0.000),the residual volume(RV)(270.0%?23.6% vs 188.0%?19.8% predicted;t=16.835,P=0.000),and the total lung capacity(TLC)((123.0%?9.8%) vs 102.0%?8.7% predicted;t=10.135,P=0.000).Postoperative complications included air leakage in 10 patients,lung infection in 5 patients,and bronchial asthma in 3 patients,all of whom were cured after symptomatic management.A follow-up was conducted in 37 patients for 3~77 months(mean,46 months).Three patients died of lung cancer at 6,10, and 20 postoperative months,respectively.Two patients died of esophageal cancer at 3 and 8 postoperative months,respectively.And one patient died of cerebrovascular disease at 6 postoperative months.The remaining 31 patients survived. Conclusions Proper perioperative management is of great importance for a successful surgery and a rapid postoperative recovery.

Objective To explore the feasibility of laparoscopic subtotal cholecystectomy without using titanium clips.Methods Laparoscopic subtotal cholecystectomy was performed without using titanium clips on 48 patients,who had serious adhesions at the Calot's triangle.The cystic duct was ligated by silk.Results The operations were completed uneventfully in all the cases without converting to open surgery.No massive hemorrhage or biliary leakage occurred during the operation.The patients were followed up for 3 months to 3 years(mean,18 months),during which no one developed abdominal pain,fever,or jaundice.Conclusions Laparoscopic subtotal cholecystectomy without using titanium clips is a safe and simple method for patients with serious adhesions at the Calot's triangle.The rate of postoperative complications is low.

Objective Assess the value of intraoperative palmar temperature and brachial artery blood flow monitring during sympathectmy. Methods 150 cases palmar hyperhidrosis underwent transthoracic endoscopic sympathectomy. Palmar temperature was all monitored and brachial artery blood flow is also detected by ultrasonography in 33 cases. Results Palmar temperature increase (2.8?1.0)℃ after sympathetic chain has been destroyed [(include a high level increase of (3.24?0.20)℃ in 125 cases and a low level increase of (0.64?0.12)℃ in 25 cases]. Ultrasonography indicate brachial artery radius (R) and blood flow volume (VOL) are significantly increased, Maximum (Vmax) and Minimum (Vmin) speed of blood flow at systole and diastole, pulse index (PI) and systole/diastole blood flow ratio (S/D) are also increased (P

Objective To investigate clinical effects of video assisted thoracoscopic lung volume reduction surgery(LVRS) for severe emphysema. Methods Six patients with severe emphysema underwent lung volume reduction surgery by video assisted thoracoscopy.The LVRS was performed unilateraly in 4 and bilateraly in 2 through median stemination.20%～30% of total volume of lung was resected. Results There was no operative death.All patients were followed up for 3 to 17 months.After LVRS,the mean forced expiratory volume in 1 second(FEV 1) and PaO 2 increased by 24 6% and 8 3%,respectively,Total lung capacity(TLC),residual volume(RV) and ventilatory assistance decreased by 24 6%,20 3% and 47 1% respectively Conclusions LVRS by video-assited thoracoscopy is an effective and safe technique for patients with severe emphysema.It can relieve dyspnea and improve excise tolerance and the quality of life.

Purpose:To study the expression and clinical significance of p27 in esophageal carcinoma. Methods:Immunohistochemical staining was used to determine the expression of p27 in 32 cases of normal esophageal issues and 88 cases of esophageal carcinoma.Results:The positive incidences of P27 expression in esophageal carcinoma was 40.9% while in normal esophageal issues it was 75.0%. The expression of p27 was correlated to degree of differentiation of carcinoma and lymph node metastasis, but not to length, depth of invasion and TNM stage. The p27 negative group was seen to have the worst survival rate among all groups.Conclusions:The results suggest that p27 gene plays an important role in carcinogenesis and progress of esophageal carcinoma. Expression of P27 may prove to be valuable in prognosis assessment of esophageal carcinoma.

In order to study the possible role of soluble interleukin-2 receptor (slL-2R) in the pathogenesis of vitiligo, we measured the levels of slL-2R in the sera and the tissue fluids from skin lesions and uninvolved skin from 41 patients with vitiligo, using the method of sandwich ELISA with monoclonal and polyclonal antibodies. The results showed that the overall level of serum sIL-2R from patients with vitiligo was significantly higher, than that from normal control group (P0.05). The level of serum sIL-2R from patients with vitiligo in progressive stage was significantly higher than that in stable stage (P

Objective To investigate the relationship between procoagulation of HFGL 2 and abnormality of coagulation in patients with systemic lupus erythematosus (SLE) by detecting the expression of HFGL2/fibroleukin in peripheral blood mononuclear cell(PBMC) of SLE patients. Methods A polyclonal antibody against HFGL2 was applied to detecting the expression of HFGL2 protein in 32 SLE patients and 15 healthy volunteers by immunohistochemistry. Semi-quantitative measurement of HFGL2 expression in the blood specimen was done with high multiple image analytical system(HMIAS). Results The expression of HFGL2 in PBMC from 23 active SLE patients was significantly higher than that in the controls, which showed a positive correlation with the disease activity. Conclusion The expression of HFGL2 in PBMC is probably correlated with the pathogenesis and disease activity of SLE.