Objective To study the relationship between the therapeutic principle and prescription of traditional Chinese medicine and the experimental pharmacologic effects in relation to the treatment of eczema. Methods Murine DNFB allergic contact dermatitis was used as an animal model, and was treated with 4 traditional Chinese medicinal compounds which were composed of Chinese materia medica with activities to suppress type Ⅳ allergic reaction. Results The results showed that the effect of compound Ⅲ composed of Paeoniae obovata Maxim(赤芍), Fructus Gardeniae(栀子), Herba Schizonepetae(荆芥), Herba Spirodelae(浮萍) and Poria(茯苓), possessing multiple potencies of cooling and promoting blood, clearing heat, expelling wind and eliminating dampness, was strongest among the compounds tested, and the compound up regulated the serum level of calcitonin gene related peptide which was lowered in the mice with DNFB induced dermatitis. Conclusion It is suggested that the compounds with suppressive effect on type Ⅳ allergic reaction might improve the therapeutic effect in clinical practice.

Objective Assess the value of intraoperative palmar temperature and brachial artery blood flow monitring during sympathectmy. Methods 150 cases palmar hyperhidrosis underwent transthoracic endoscopic sympathectomy. Palmar temperature was all monitored and brachial artery blood flow is also detected by ultrasonography in 33 cases. Results Palmar temperature increase (2.8?1.0)℃ after sympathetic chain has been destroyed [(include a high level increase of (3.24?0.20)℃ in 125 cases and a low level increase of (0.64?0.12)℃ in 25 cases]. Ultrasonography indicate brachial artery radius (R) and blood flow volume (VOL) are significantly increased, Maximum (Vmax) and Minimum (Vmin) speed of blood flow at systole and diastole, pulse index (PI) and systole/diastole blood flow ratio (S/D) are also increased (P

Objective To explore the mechanism of siRNA targeted hTERT gene to inhibit bladder cancer T24 cell growth by decreasing c-myc and TGF-?1 expression. Methods shRNA-hTERT-pTZU6+1 vectors were constructed by RNAi-DNA vector technique, then the vectors were transfected into bladder cancer T24 cells,and the most effective vector and its optimal concentration were screened using RT-PCR to detect hTERT expression in T24 cells.The T24 cell growth, the alternative of cell phase,the expression of hTERT,c-myc and TGF-?1 were detected by flow cytometry,RT-PCR and immunohistochemistry. Results Three shRNA-hTERT-pTZU6+1 vectors were successfully constructed.The most effective vector was ph2-shRNA vector,and its optimal concentration was 1.0 ?g.This vector decreased the cell growth and the cell number of S phase from 65.2% to 38.6%,increased the cell number of G0/G1 phase from 32.0% to 57.9%,and attenuated both mRNA and protein expressions of hTERT,c-myc and TGF-?1 in T24 cells. Conclusions Targeted hTERT gene with siRNA may inhibit the cell proliferation of bladder cancer;down-regulating hTERT expression by attenuating the expression of c-myc and TGF-?1 is probably involved in the mechanism.

Objective To explore the feasibility of laparoscopic subtotal cholecystectomy without using titanium clips.Methods Laparoscopic subtotal cholecystectomy was performed without using titanium clips on 48 patients,who had serious adhesions at the Calot's triangle.The cystic duct was ligated by silk.Results The operations were completed uneventfully in all the cases without converting to open surgery.No massive hemorrhage or biliary leakage occurred during the operation.The patients were followed up for 3 months to 3 years(mean,18 months),during which no one developed abdominal pain,fever,or jaundice.Conclusions Laparoscopic subtotal cholecystectomy without using titanium clips is a safe and simple method for patients with serious adhesions at the Calot's triangle.The rate of postoperative complications is low.

Objective To study the X-ray and CT features of costal eosinophilic granuloma for a better understanding.Methods Eight patients with costal eosinophilic granuloma proved by surgery or biopsy were analyzed retrospectively.All patients had X-ray plain film, 6 patients had CT examination,including a case of enhanced CT scan.Results All 8 lesions were solitary.Six lesions were in the anterior rib and 2 in the posterior rib.On X-ray, all case showed single cavity and oval lesion with clear boundary.On CT images, 5 lesions demonstrated expansile destruction of bone with cortical bone thinning, and 3 were osteolystic destruction with soft tissue mass around.On the patient with enhanced CT scan, the lesions showed a moderate and uniform enhancement.Conclusion The X-ray and CT findings of costal eosinophilic granuloma are characteristic.

Objective To summarize the experience of perioperative management of lung volume reduction surgery(LVRS) under video-assisted thoracoscopy.Methods A total of 40 patients with severe chronic obstructive pulmonary disease(COPD) underwent LVRS under video-assisted thoracoscopy.A mini-incision thoracotomy was given in 23 of them.During the operation,the Endo-GIA was utilized to resect 20%~30% of total volume of affected lung.Results The LVRS was performed in bilateral lungs in 13 patients(under thoracoscopy only in 7 patients and with the assistance of mini-incision thoracotomy in 6 patients) and in unilateral lung in 27 patients(under thoracoscopy only in 10 patients and with the assistance of mini-incision thoracotomy in 17 patients).There were no surgery-related deaths.All the patients were discharged from the hospital 9~41 days(mean,18 days) after operation.Not only symptoms of dyspnea were obviously relieved but also the respiratory index was upgraded by 1~2 grades.The comparisons on pulmonary functions between preoperative time and 1 month after operation indicated a great improvement postoperatively,including the forced expiratory volume in 1 second(FEV_1)(48.3%?4.9% vs 68.5%?5.6% predicted;t=17.169,P=0.000),the residual volume(RV)(270.0%?23.6% vs 188.0%?19.8% predicted;t=16.835,P=0.000),and the total lung capacity(TLC)((123.0%?9.8%) vs 102.0%?8.7% predicted;t=10.135,P=0.000).Postoperative complications included air leakage in 10 patients,lung infection in 5 patients,and bronchial asthma in 3 patients,all of whom were cured after symptomatic management.A follow-up was conducted in 37 patients for 3~77 months(mean,46 months).Three patients died of lung cancer at 6,10, and 20 postoperative months,respectively.Two patients died of esophageal cancer at 3 and 8 postoperative months,respectively.And one patient died of cerebrovascular disease at 6 postoperative months.The remaining 31 patients survived. Conclusions Proper perioperative management is of great importance for a successful surgery and a rapid postoperative recovery.

Objective To investigate clinical effects of video assisted thoracoscopic lung volume reduction surgery(LVRS) for severe emphysema. Methods Six patients with severe emphysema underwent lung volume reduction surgery by video assisted thoracoscopy.The LVRS was performed unilateraly in 4 and bilateraly in 2 through median stemination.20%～30% of total volume of lung was resected. Results There was no operative death.All patients were followed up for 3 to 17 months.After LVRS,the mean forced expiratory volume in 1 second(FEV 1) and PaO 2 increased by 24 6% and 8 3%,respectively,Total lung capacity(TLC),residual volume(RV) and ventilatory assistance decreased by 24 6%,20 3% and 47 1% respectively Conclusions LVRS by video-assited thoracoscopy is an effective and safe technique for patients with severe emphysema.It can relieve dyspnea and improve excise tolerance and the quality of life.

Objective To assess the outcomes,complications,and quality of life following endoscopic thoracic sympathectomy(ETS) of the T2~T4 ganglia for treating primary palmar hyperhidrosis(PH). Methods A total of 200 cases were followed with clinic checkups or telephone inquiries for a period of 1~28 months(mean,18.4 months). Results Of the 200 cases,8 cases were loss of follow-up and 192 cases obtained a complete and fully satisfactory alleviation of palmer and axillary hyperhidrosis.No recurrence and severe complications were found.The prime complication was compensatory hyperhidrosis(52/192,27.1%),whose incidence was slightly higher in female and severe patients and unrelated to age.Other complications included 1 case of pneumothorax and 1 case of pulmonary atelectasis. Conclusions Endoscopic thoracic sympathectomy of the T_2~T_4 ganglia for palmar hyperhidrosis is effective,safe and reliable.The procedure improves patients' quality of life and gives satisfactory outcomes.However,all patients should be informed of the common complications,particularly compensatory hyperhidrosis,before the surgery.

Objective To summarize the experience of 200 cases of endoscopic thoracic sympathectomy(ETS). Methods Clinical materials of T_2~T_4 sympathectomy under thoracoscope for treating palmar hyperhidrosis from January 2003 to April 2005 were analysed. Results [WTBZ] All the 200 cases of operation were successfully completed.Symptoms of hyperhidrosis vanished with both hands dry and warm in all the patients.Plamar temperature increased by a mean of(3.1?0.9)℃ after operation.Follow-up observations in 192 cases for 1~28 months(mean,18.4 months) found no recurrence.Compensatory hyperhidrosis was found in 52 cases(27.1%). Conclusions Endoscopic thoracic sympathectomy is a safe,effective,and micro-invasive treatment for palmar hyperhidrosis.

Through investigation on the cognition and status of the cultivation of innovative ability of the students,we found that the innovative ability of medical students was extremely weak.Their practical ability was out of joint to the cognition of innovative ability.We are trying to analyze the reasons and resolve the problem.