1.Comparison of 16-slice spiral CT and MMP-9 in diagnosis of invasion and metastasis in hman esophageal cancer
Chinese Journal of Primary Medicine and Pharmacy 2008;15(8):1337-1338
Objective To compare the clinical values of 16-slice spiral coroputed tomography(CT)and matrix metalloproteinases-9(MMP-9)in diagnosis of invasion and metastasis in human esophageal cancer.Methods Thirty-eight patients with esophageal cancer were detected with spiral CT,and their tumor tissues were detected for expression of MMP-9.Results 12 cases were diagnosed for vicinitic tissues damages by histopathological examination,while 11 cases were regarded to be damaged by CT and 13 cases were done according to MMP-9 determination;as to metastasis,there were 13 casese by histopathological examination,12 cases by CT and 16 cascs by MMP-9 determination.No statistical difference were observed in diagnosis of vicinitic tissues damages and metastasis by the three methods in positive rates,sensitivity,accuracy,specificity and diagnosis accordance rates(P>0.05).Conclusion It is the important method for spiral CT in diagnosis of esophageal cancer,which could not be replaced by others in judgement of invasion and metastasis.
2.Clinical value of 16-slice spiral CT in diagnosis of colorectal carcinoma
Chinese Journal of Primary Medicine and Pharmacy 2008;15(2):293-294,后插4
Objective To explore the clinical value of 16-slice spiral CT and its post-processing technics in diagnosis of colorectal carcinoma.Methods CT characteristics,surgery and pathological data of forty-three patients with colorectal carcinoma were analyzed retrospectively.Results The accurate diagnosis rate using 16-slice spiral CT was 100%,a total of forty-five focus were observed by CT and its post-processing pictures.It is safe to detect colorectal carcinoma using spiral CT without wounds.Further more,tumors and its growth towards enteric cavity,wall thichness,damaged surroundding tissues were all observed clearly as well as metastasize.Conclusion 16-slice spiral CT and its post-processing technics is effective in diagnosis of colorectal carcinoma,which should be used in clinical experiences.
3.Miyalisan Compared with Ribavirin in the Treatment of Infan ts'Diarrhea
Herald of Medicine 2001;(4):228-
To compare the efficacy on miyalisa n with ribavirin in the treatment of infants' diarrhea. Methods: 152 infants with diarrhea in outpatient department were divided into two groups treated with miyalisan (80 cases) or ribavirin (72 cases) for 3 days. The therapy could be continued for another 3 days if necessary. Results : The Clinically effective rate and the negative conversion rate of rotavirus in the treated group and controlled group were 93.8% and 87%, 82% and 60%, respectively, there were sinnificantly different(P<0.05) between two g roups. Conclusion: Miyalisan had showed a better efficacy in treatment of infants' rotavivus enteritis than ribavirin.
4.In vive MR evaluation of the vessel wall shear stress in the common carotid artery
Binbin SUI ; Peiyi GAO ; Yan LIN
Chinese Journal of Radiology 2008;42(8):854-857
Objective To apply a non-invasive and feasible method for the quantification of local wall shear stress (WSS) in vivo using magnetic resonance imaging. Methods The fight common carotid artery of a young healthy male volunteer was examined using cine phase-contrast MR sequence. The cross- sectional area, average flow velocity, maximum velocity and flow rate were obtained. Three dimensional paraboloid model was applied to measure WSS value at common carotid artery. Results The mean/peak WSS was (0.75±0.41)N/m2 for the common carotid artery; The mean (range) velocity was (23.4± 12.0) cm/s; The mean (range) luminal vessel area was (32.2±2.9) mm2; The blood flow rate was (7.8±4. 6) ml/s; Conclusion WSS's magnitude, distribution and changes can be determined by MR imaging combining with the three-dimensional paraboloid method.
5.Studies on quality standard of ADUOLA FUZHENGLIN CAPSULE(Ⅰ)
Sui LIN ; Sishan DENG ; Ren LIU ;
Chinese Traditional and Herbal Drugs 1994;0(06):-
Object To specify a standard for the quality control of ADUOLA FUZHENGLIN CAPSULE * Methods The content of astragaloside Ⅳ in ADUOLA FUZHENGLIN CAPSULE was determined by TLCS Results The method showed linearity within the range of 1 ?g to 5 ?g, the average recovery rate was 97 82%, and RSD was 3 56% Conclusion This method is simple and highly reproducible, and may be used for the quality control of the CAPSULE
6.Investigation on Fluorine Contents of Well Water in Endemic Fluorosis Area of Hejian City
Jianlin CUI ; Chunsheng SUI ; Lin YE
Journal of Environment and Health 1992;0(05):-
Objective To understand the relationship between the fluoride contents and the total hardness, pH value, the depth of well in the endemic fluorosis areas of Hejian city. Methods 40 villages in 2 towns of Hejian city were selected randomly and the well water samples in these villages were collected and determined. Results The contents of fluoride in the well water were higher than the limit(1 mg/L) in Sanitary Standard for Drinking Water Quality (China, 2001), ranged 1.2~4.4 mg/L, with a mean of 2.80 mg/L. The fluoride contents increased as the total hardness decreased (r=-0.615, P
7.Investigation of Perioperative Prophylactic Utilization of Antibiotics in Cataract Patients and Its Rationality Evaluation
Ping LI ; Yuefen LIU ; Lin WANG ; Zhongguo SUI
China Pharmacy 2007;0(30):-
OBJECTIVE:To investigate the perioperative prophylactic utilization of antibiotics in cataract patients in ophthalmology department of our hospital.METHODS:100 cases of cataract surgery in 2009 were randomly collected from our hospital.Category of antibiotics,administration time point,medication time,wound healing and drug costs in hospitalization period were analyzed statistically and the rationality of above indexes was evaluated.RESULTS:100 cases all received prophylactic antimicrobials by local and systematic administration in the perioperative period(100%).Drugs for systematic administration contained Levofloxacin(n=67,67%),Cefuroxime sodium for injection(n=25,25%),Clindamycin phosphate injection(n=2,2%) and Ceftazidime for injection(n=6,6%).63 cases were only given drugs 30 min before operation(63%);12 cases were given medicine 30 min before operation and within 24 h after operation(12%);above situation accounted for 75%.25 cases were given medicine before operation and within 24 h after operation(25%),in which the average medication duration was 2.5 days and the longest was 4 days.CONCLUSION:The irrational use of drugs in our hospital manifests inappropriate selection and too long use of antibiotics after operation.The interference of perioperative prophylactic utilization of antibiotics should be reinforced and it is urgent to intensify consciousness of physicians about rational use of antibiotics in perioperative period.
8.Effects of Zibu Piyin Recipe Serum on endoplasmic reticulum(ER)stress-induced neuron apoptosis and its mechanism
Libin ZHAN ; Xiaoguang LU ; Haiyan LIN ; Hua SUI ; Xiaoyang CONG
Chinese Journal of Emergency Medicine 2008;17(12):1280-1284
Objective To explore the inhibitory effects of Zibu Piyin Recipe(ZBPYR)serum on neuron apoptosis induced by tunieamyein(Tm,5 μg/ml)and its mechamsm in vitro by using sero-pharmacological method.Method Totally 12 healthy adult male SD rats(220~250 g)(SPF)were divided randomly into control group and ZBPYR group,6 in each group,then the blank and ZBPYR serum were prepared.The mouse.neuroblastoma cell line Neum2a cells were treated with Tunicamycin(Tin,an inhibitor of N-glycoslytion)to establish the endoplasmic reticulum(ER)stress model.The cells treated by ZBPYR aerum of different concentrations were interventional groups,and the cells treated by blank serum were control group.The viability of Neuro2a cells was meusurcdd by MTT assay.Flow cytometry wus applied to observe the apoptosis of Neuro2a cells.Western blotting was utilized to detect the protein expressions of two molecules,ER molecular chaperone-ucose regulated protein 78(CRP78)and transcriptional factor CCAAT/enhancer-binding protein-homologous protein(CHOP).The results were analyzed by sNK-q test.Results Compared to Tm group(cell viability 0.1673±0.0213,apoptotic rate 62.7050±1.4056),The cell viability of interventional groups(5%0.5295±0.0373,10%0.5843±0.0428,15%0.6274±0.0324)increased significantly(P<0.05);and the apoptotic rate(5%47.8733±2.8166,10%46.3366±1.2748,15%39.8833±1.0524)reduced significantly(P<0.05).The protein expressions of GRP 78(5%2.1228±0.2251,10%1.3293±0.9443,15%;15%0.0931±0.1168)and CHOP(5%1.1776±0.2927,10%0.7290±0.1708,15%0.6577±0.1883)of interventional groups reduced significantly compared with Tm group(GRP78 2.9149±0.5355;CHOP 1.6611±0.2913)P<0.05.Condusions ZBPYR serurn could increase the cell viability of Neuro2a cells treated with Tm and inhibit cell apoptosis.Thereby it may have neuroprotective effects,and the mechanism may be associated with the inhibition of ER stress and apoptosis pathway.
9.Fastigial cerebellar nucleus may be involved in the pathogenesis of post-stroke depression through the cerebellum-hypothalamic pathways in rats Fastigial cerebellar nucleus may be involved in the pathogenesis of post-stroke depression through the cerebellum-hypothalamic pathways in rats
Yuan LI ; Rubo SUI ; Xin ZHANG ; Yuhan LIN
Journal of Xi'an Jiaotong University(Medical Sciences) 2015;(2):164-168
ABSTRACT:Objective To investigate whether medial cerebellar nucleus may be involved in the pathogenesis of post-stroke depression (PSD)and explore the pathway that mediates this effect.Methods Healthy SD rats were randomly divided into five groups:control group,stroke group,PSD group,medial cerebellar nucleus (Med)lesion group,and superior cerebellar peduncle (xscp)lesion group.The ethological score of depression was evaluated.The neurotransmitters of Glu and GABA in the lateral hypothalamic area were detected by high performance liquid chromatography.Results Compared with those in control group,the levels of GABA and Glu expressions did not significantly differ in the lateral hypothalamic area in stroke group (P > 0.05 ).In contrast,the levels in PSD group,Med lesion group and xscp lesion group were lower than those in stroke group to different extent (P <0.01). Conclusion These results preliminarily show that medial cerebellar nucleus may participate in the pathogenesis of post-stroke depression,which may be mediated by the cerebellum-hypothalamic pathway.
10.Construction of multiple antigenic peptide and the immunity analysis
Xuai LIN ; Yujuan SUI ; Yafei MAO ; Jie YAN
Chinese Journal of Microbiology and Immunology 2008;28(11):1010-1013
Objective To construct the multiple antigenic peptide (MAP) gene and E. coli ex-pression system, based on the out membrane protein OmpL1, LipL21 and LipL32 from Leptospira interro-gans, and better understanding of the immunological activity of the recombinant protein. Methods Using MI3KE display and Western blot, the advantage epitopes of OmpL1, LipL21 and LipL32 were identified and used to synthesize a new gene, then its prokaryotic expression system was constructed. The expression of re-combinant protein was determined by SDS-PAGE. The immunity activity of the recombinant protein was iden-tified by Western blot and ELISA. Results The synthetic gene was effectively expressed in E. coli and mainly presented in soluble form. The expression protein could react with the antileptospirosis antibodies in rabbit and human sera, which contained different serogroups. Conclusion The recombinant MAP gene of leptospires was successfully constructedand and expressed in E. coli. The recombinant protein had a good immune activity, and could cross-reacted with antileptospirosis antibodies from different serogroups.