Objective:To investigate the enhancement of radiosensition of COX-2 inhibitor Nimesulide on lung cancer cell line (A549) in vitro and try to reaserch the possible radiosensitizing mechanisms. Methods:A549 cell line which is in logarithmic phase was devided into four group:control group,drug group,radiation group and case group. The apoptosis and the expression in protein of Bcl-2 and Bax were measured by flow cytometry(FCM). The enhancement of radiosensitising effect was detect by clone formation experiment. Results:Low dose of Nimesulide combined with X-ray can induce the up-regulation of the protein of Bax and inhibit the expression of the protein of Bcl-2,that increased the apoptosis of tumour cell. The difference is significant. Conclusion:It is suggested that Cox-2 inhibitor-Nimesulide enhance the radiosensitivity to the cell line A549 of adenocarcinoma of lung.

Objective: To explore the effect of telmisartan combined with amiodarone on the levels of serum brain natriuretic peptide (BNP), matrix metalloproteinase-2 (MMP-2) and connective tissue growth factor (CTGF) in the patients with hypertension complicated with paroxysmal atrial fibrillation.Methods: A total of 90 hypertensive patients with paroxysmal atrial fibrillation were randomly divided into amiodarone+ telmisartan group and amiodarone group.The 45 patients in amiodarone group were treated with amiodarone hydrochloride and the 45 patients in amiodarone+telmisartan group were treated with telmisartan combined with amiodarone.After 12-month treatment, the clinical effect, changes of blood pressure, heart rate, left atrial volume, recurrence frequency and duration time of atrial fibrillation were compared between the groups, and the levels of serum BNP, MMP-2 and CTGF before and after the treatment were detected.Results: The total effective rate of amiodarone+telmisartan group was 95.6%, which was significantly higher than that of amiodarone group (75.6%, P<0.05).The blood pressure, recurrence frequency and duration time of atrial fibrillation were significantly improved after the treatment in the two groups (P<0.05).The blood pressure, left atrial volume and situation of atrial fibrillation in amiodarone+telmisartan group were better than those in amiodarone group after the treatment (P<0.05).The levels of serum MMP-2 and CTGF were significantly reduced in the two groups after the treatment, and the levels of BNP, MMP-2 and CTGF in amiodarone+telmisartan group were significantly lower than those in amiodarone group (P<0.05).The incidence of adverse reaction was not significantly different in the two groups (P>0.05).Conclusion: Telmisartan combined with amiodarone in the treatment of hypertension complicated with paroxysmal atrial fibrillation shows promising curative effect, which can inhibit the left atrial expansion, decrease the frequency and duration time of atrial fibrillation and reduce the levels of serum BNP, MMP-2 and CTGF.

Interferon-γ-inducible protein 10 (IP-10),also known as chemokine (C-X-C motif) ligand 10(CXCL10),belongs to the CXC subfamily.IP-10 exerts its function by binding to chemokine (C-X-C motif) receptor 3 or Toll-like receptor 4.IP-10 plays an important role in the occurrence and development of autoimmune diseases.The expression of IP-10 in blood and body fluid is closely associated with the activity of autoimmune disease.IP-10 is expected to become a potential new marker and possess a significant application value in early screening,condition monitoring and therapeutic evaluation of autoimmune diseases.

Objective To establish chemiluminescent immunoassay(CLIA) for quantitative detection of Rabies virus antibody in human serum.Method The purified antigen was coated on the microplate,pairing with alkaline phosphatase(ALP)-labeled antigen and luminescence substrate CSPD,then the double antigen sandwich CLIA was established.Results The sensitivity of the assay was 0.2IU/ml.The linear range was from 0 IU/ml to 200 IU/ml.The precision was less than 10%.The analytical recovery rate was from 90% to 110%.Conclusion The CLIA is a simple,sensitive,special and repeatable method for detection of Rabies virus antibody.It was suitable for clinical application.

Purpose To study the diagnostic value of dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI),diffusion tensor imaging (DTI) and the combination of these two techniques in benign and malignant nodules of central prostate gland.Materials and Methods Forty-nine patients with nodular abnormal signal in central prostate gland who had accepted prostate MRI scan in Shihezi University Medical College First Affiliated Hospital from September 2015 to December 2016 were retrospectively analyzed,and all the subjects had accepted MRI T2WI,DTI and DCE scan.The pathological results confirmed that there were 21 prostate cancer (PCa) and 36 benign prostatic hyperplasia (BPH) among 57 central gland nodule samples.The fractional anisotropy value,apparent diffusion coefficient value,time to peak,SImax,the curve slope and timesignal intensity curve of nodules were respectively detected,the differences of the parameters between PCa group and BPH group were compared,and receiver operating characteristic (ROC) curve analysis was carried out.Results The difference of fractional anisotropy value,apparent diffusion coefficient value,time to peak and R between PCa group and BPH group was statistically significant (P＜0.05),and there was no significant difference in SImax (P＞0.05);the time-signal intensity curve type of PCa group was mainly in the form of fast-increasing and then decreasing,while the curve type of BPH group was mainly in the form of platform.The area under the ROC curve of DCE,DTI and the combination of DEC and DTI were 0.87 (95％ CI:0.751-0.942),0.85 (95％ CI:0.734-0.933) and 0.94 (95％ CI:0.837-0.983),respectively.Conclusion In the cases of diagnosing benign and malignant nodules of central prostate gland,the accuracy of DTI and DCE was lower than that of the combination of these two techniques,and the diagnostic effect of DTI combined with DCE was better.

Sixteen patients underwent fasciocutaneous flap pedicle with the perforating branches of posterior tibial artery to repair the skin defects of the middle and lower leg in Department of Orthopaedics,Fuzhou General Hospital of Nanjing Military Area Command of Chinese PLA from February 2003 to February 2007.Based on anatomy,the flap axis ray was designed as the connection of internal condyle of tibia and the middle point between inner malleolus and achilles tendon,rotating along the axis ray adjacent to wound with pedicle having perforating branches of posterior tibial artery.Under guidance of Doppler flowmeter,all skin including flap,pedicle,and surface of perforating branches must be fine.The upper leg skin defect was repaired using anterograde flap,and the middle-lower leg skin defect was repaired using retrograde flap,including lower leg defect in 9 cases,middle leg defect in 3 cases and upper leg defect in 4 cases.The skin defects ranged from 2.0 cm?1.5 cm to 12.5 cm?7.5 cm,and the largest size of flap was about 15.0 cm?10 cm,and the smallest size of flap was about 4.0 cm?2.5 cm.The repairing effect was observed.All flaps survived.Two cases developed distal necrosis of skin,but granulation was found in subcutaneous tissue,of which 1 case healed after changing dressings,and the other recovered after skin grating.All subjects were followed up for over 6 months.All cases could walk naturally but felt pain.Fasciocutaneous flap pedicle with the perforating branches of posterior tibial artery can repair the skin defects of leg soft tissue.The donor site is posterior leg.The pedicel is kept well after injury,and the flap is easy to design.In addition,the blood supply is reliable,and survival rate is high.It is a good surgery to repair leg soft tissue defect.

With the further study on molecular pathogenesis of multiple myeloma (MM),lots of novel targeted therapies emerged into clinical trials,which improved the response rate and life quality of MM patients.In the 56th American Society of Hematology (ASH) annual meeting,a series of special reports about latest developments in targeted therapy for MM patients were published.Efficacy was improved significantly by these reagents,which may provide the new treatment strategies for MM patients.The advances in the novel targeted therapies will be summarized in this paper based on the new reports in the 56th ASH annual meeting.

Individual susceptibility to cancer induced by environmental agents may be influenced by polymorphic metabolic genes responsible for the activation or detoxification of carcinogens．The association between genetic polymorphisms in cytochrome P4501A1 (CYP1A1) and lung cancer susceptibility has been extensively studied．The various CYP1A1 alleles exhibit population frequencies that depend on race and ethnicity．An increased risk of lung cancer in Asians was found to be associated with genetic polymorphisms in CYP1A1．However，reports from Caucasians are not consistant，probably suggesting the ethnic-sepecific effect of the polymorphisms in the locus on the cancer．Evidence also exists for the association between levels of carcinogen-DNA adducts or frequency of oncogene and tumor suppressor gene mutations and CYP1A1 polymorphisms．These findings provide a better understanding of the relationship between CYP1A1 and lung cancer susceptibility．

Objective To observe the relation between content of type Ⅲ collagen and expression of decorin in paraurethral connective tissue in patients with stress urinary incontinence(SUI). Methods Transvaginal biopsies were obtained from the paraurethral connective tissue in 43 SUI and non-SUI control women. The concentration of type Ⅲ collagen was determined by immunohistochemical technique, electrophoretic separation and quantification. RT-PCR was used to verify the mRNA level of decorin. Results The mean concentration of type Ⅲ collagen was significantly reduced in patients with SUI(23?4), compared to the control group(34?6, P

There are many ways to treat pathological scars.Silicon gel sheeting as a noninvasive and convenient method, are widely used in clinic.The article reviews the lituratures on theoretical research of silicon gel sheeting and its efficacy in the past 26 years, and analyzes the effectiveness of silicon gel sheeting for comparison with other methods.