Objective To investigate the possible mechanisms of ox-LDL on vascular smooth muscle cells (VSMC) with and without exogenous zinc finger protein A20 gene. Methods VSMC were trans-fected with plamid containing A20 gene and incubated with ox-LDL. TLR4 and LOX-1 mRNA expression were measured by RT-PCR. Results After VSMC incubated with ox-LDL, the expression of LOX-1 and TLR-4 mRNA reached a higher level. However, when VSMC were transfected with plasmid containing A20 gene, the NF-kB nuclear translocation and the ability of migration reduced to normal level. Conclusions ox-LDL can significantly activate NF-kB signaling system and enhance migration in VSMC. The activation may induce the inflammatory response in arterial wall, the migration of VSMC to the intima, and triggering the process of atherosclerosis. Transfecting plasmid containing A20 gene significantly inhibited the activation of NF-kB and the ability of migration of VSMC. A20 effects might be through inhibiting NF-kB signaling. Meanwhile, A20 blocked the development of atherosclerosis.

Hyperthyroidism with periodic paralysis is common in clinic,the curative effect of peroral drugs on it is not satisfactory. In order to evaluate the treatment of 131 I for periodic paralysis in patients with hyperthyroidism, fouty two patients with hyperthyroidism complicated with periodic paralysis were put on p.o. 5.0 9.0 mCi 131 I Na treatment and the remission rate of periodic paralysis was observed.The total effective rate was over 95%. It can be concluded that 131 I could be used as a routine therapy for periodic paralysis in most patients with hyperthyroidism for its safety and efficiency.

Ultrasonic scalpels,including incision type and aspiration type,are introduced from aspects of the principles,key technologies and clinical application.Uitrasonic scalpel has the advantages of less bleeding,exact incision,,safety,shortened time of operation and less damage to surrounding tissue.Some technology problems of ultrasonic scalpel are also summarized.It is pointed out that ultrasonic scalpel is the tendency and direction of clinical surgery development,which has extensive application prospects.

OBJECTIVE: As a new method of altitude training, stimulated living high-training low training (HiLo), can solve the problems in traditional altitude training. In this paper, we review the effect of simulated living high-training low on the related indexes of red blood cells and the expression of erythropoietin and hypoxia inducible factor-1mRNA to improve the development and application of HiLo, and make it better service for our athletes. DATA SOURCES: A computer-based online search of PubMed was undertaken for the articles published between January 1972 and December 2005, among which partial articles were retrieved according to relevant references with the keywords of "simulated living high-training low training (HiLo), indices of red blood cell, effect; erythropoietin, hypoxia inducible factor-1; gene". Meanwhile, we searched http://cnki.hunnu.edu.cn for the relevant articles published between 1996 and 2006 with the same key words in Chinese. STUDY SELECTION: The articles that involved the effect on the indices of red blood cell and the expression of erythropoietin and hypoxia inducible factor-1 mRNA in the model of stimulated living high-training low training through the first trial sport were chosen after the first selection. Then, the full texts of these articles were looked up to identify whether they were the relevant studies. Inclusive criteria: ①randomised controlled experiments; ②the experiment including control and intervention groups. Exclusive criteria: ①review articles; ②repetitive studies. DATA EXTRACTION: Totally 90 relevant articles were collected, of which 38 were accordant with the inclusive criteria and the 52 repetitive studies or review articles were excluded. DATA SYNTHESIS: ①Levine discovered that in HiLo the maximal O2 consumption (VO2max) in the training group was increased 5% in the mean and red cell 9% and the ability of exercise was largely improved, while the results of the control group had no significant changes. ②HiLo training can induce the production of hypoxia inducible factor-1, which regulates erythropoietin, and its production. The increase in erythropoietin triggers an increase in red cell mass and Hb concentration. This hematological acclimatization response facilitates the restoration of blood O2 content and improves tissue oxygenation. Continuous residence at moderate heights (over 2 000-2 500 m) could improve the oxygen transport capacity, augment VO2 max and enhance exercise performance by increasing the content of erythropoietin and hemoglobin concentration. ③HiLo can induce the expression of hypoxia inducible factor-1, but the problems that how it regulates the expression of erythropoietin and transport the signal, and HiLo can alert the portion of blood, which affects the ability of exercise still need further study. Then we will more clearly learn the mechanism of the effect of HiLo on the ability of athletes, and help to enhance their performance. CONCLUSION: HiLo can increase the expression of erythropoietin and hypoxia inducible factor-1mRNA and the generation of red blood cells, which leads to an increase in hemoglobincon concentration and hematocrit, and effectively improves the ability of athletes.

Objective To observe the CT features of mycoplasma pneumoniae pneumonia. Methods CT features of 40 adult patients with serologically proven mycoplasma pneumoniae pneumonia were retrospectively reviewed. Results The most common CT findings of mycoplasma pneumoniae pneumonia were ground-glass opacity (34/40, 85.00%) and consolidation (28/40, 70.00%). The other findings included nodules (18/40, 45.00%), thickening of the bronchovascular bundles (6/40, 15.00%), interlobular septal thickening (6/40, 15.00%), mediastinal lymphadenopathy (6/40, 15.00%) and pleural effusion (4/40, 10.00%). Most of the ground-glass opacity and consolidation showed tendency of lobular distribution and involving the lower lobes. In some patients, the absorption of the lesions was slower than the relief of symptoms. Conclusion CT findings of mycoplasma pneumoniae pneumonia have some features, and can provide help for the diagnosis of mycoplasma pneumoniae pneumonia.

Humans ; Methyl Ethers ; toxicity ; Occupational Exposure

Objective The measurement of normal tibia was aimed to obtain the value of the posterior slope angle of the medial tibial plateau in Chinese adult and its related parameters in order to improve the design of knee prosthesis more suitable for Chinese people, and also provide an essential features of tibia for resecting tibial plateau in total knee arthroplasty. Methods 450 knees of 428 persons which were healthy Chinese adult long living in North China, were 252 male knees and 198 female knees with an average age of 43.9 years ranging from 18 to 79 years. The criteria of the subjects were no tibial deformity, no genu varum and valgum, abnormal changes of articular surface, and had sustained ever any tibial trauma. Depending on the age of subjects, they were divided into three groups, group A was between 18 and 39 years, group B between 40 to 59 years, and group C more than 60 years. Moreover, 6 tibias harvested from healthy Chinese corpses were measured as well. Computer radiography was used to take standard lateral aspect of X-ray film. The imagines were stored into computer by scanning the films, and so the measurement was taken in computer using Danzig method. The data were analyzed statistically. Results By two factors analysis, the sex and age had no significant influence of the posterior slope angle of the tibia; furthermore, the angle was not different between the left and right tibia. In male adult, the average degrees of left posterior slope angle of the medial tibial plateau were 13.2??4.4?, the right 10.5??5.1?, and the average degrees of posterior slope angle of both sides were 11.7??4.9?. In female adult, the average degrees of left posterior slope angle of the medial tibial plateau were 11.2??3.5?, the right 10.6??4.4?, and the average degrees of posterior slope angle of both sides were 10.8??4.0?. The average degree of group A were 11.7??4.4?, group B 10.4??4.7?, group C 12.3??4.4?. In summary, there was no significant difference of posterior slope angle of the medial tibial plateau between sex, side and age. Conclusion The average degrees of posterior slope angle of the medial tibial plateau in North China adult were 11.3??4.5? ranging from 0??21? to 9?-13?, and were larger than that of the Westerns.

Objective To explore the clinical results of internal fixation of patellar transverse body fracture by comparing figure-of-eight tension band wiring combined with cannulated compression screws technique with traditional modified tension band (AO technique). Methods There were 14 cases in the cannulated screws combined with tension band wire group, it involved 9 males and 5 females, the average age was 46 years. The patients were followed up for an average of 30 weeks with a range from 16 to 48 weeks. Another 20 cases were in the modified tension band group, it involved 13 males and 7 females, the average age was 42 years. The patients were followed up for an average of 42 weeks with a range from 36 to 72 weeks. The t test was used to compare the clinical results between two groups, including the mean knee flexion at the 2nd and 4th week, the mean clinical union time and the mean flexion loss at the 24th week postoperatively. Results In the cannulated screws combined with tension band wire group, the mean knee flexion at the 2nd week and 4th week postoperatively was 59??14.8? and 98??15.1? respectively. The mean clinical union time was (10?3.0) weeks and the mean flexion loss at the 24th week postoperatively was 11??6.3? without any complication. In the traditional modified tension band group, the mean knee flexion at the 2nd week and 4th week was 31??13.3? and 63??13.7? respectively. The mean clinical union time was (13?3.8) weeks and the mean flexion at the 24th week postoperatively was 18??6.2?. In this group, 2 cases failed because loosening of the tension band with the pin pulled out. One was managed with the plaster brace, the other underwent re-operation. Conclusion With stable construct, the internal fixation of transverse patellar body fracture with figure-of-eight tension band wiring combined with cannulated compression screws is less invasive to local soft tissue, optimal on knee functional recovery and fracture union. No complication has occurred, but the long-term clinical result and complication should be followed up for longer time.

Objective To explore three dimensional relationships of commonly used bony marks of the distal femur, and to investigate its clinical application of distal femur rotational alignment. Methods One hundred and fifteen normal distal femurs of 62 volunteers were studied. With MR sagittal imaging and three dimensional reconstruction, correlative parameters, including PCA (posterior condylar angle), angle between surgical transepicondylar axis (STEA) and posterior condylar line (PCL); CTA(condylar twist angle), angle between clinical transepicondylar axis (CTEA) and PCL; APA, angle between the perpendicular of antero-posterior line (APL) and PCL; ATA, angle between the perpendicular lines of APL and STEA; CSA, angle between CTEA and STEA. All these angles were measured. The parameters of the two groups both in males and females, as well as in right and left knees were analysed. Results PCA: 3.44??1.08? (range, 0.62? to 6.75?); CTA: 6.03??0.86? (range, 2.93? to 8.92?); APA: 4.31??0.64? (range, 1.92? to 7.05?); ATA: 0.87??0.78? (range, -1.72? to 3.84?); CSA: 2.58??0.57? (range, 0.22? to 4.51?). There was no significant difference between left and right knee in terms of all the above mentioned parameters, yet there was significant difference between male and female groups in terms of PCA, CTA and ATA. PCA and CTA were larger in male groups than that of female group, while ATA in female group was larger than that of male group. Conclusion Three dimensional reconstruction based on imagings of MR is a reliable method to study the distal femur rotational alignment. In the normal knees, there is constant relationship between these parameters. Operators should use distal femur rotational alignment according to practical conditions in performing the total knee arthroplasty.

Objective To investigate the role of the expression of p27 and cyclin E in the pathogenesis of Bowen's disease and squamous cell carcinoma (SCC). Methods The expression of p27 and cyclin E was assessed by immunohistochemical method in 16 patients with Bowen's disease, 53 patients with SCC and 25 normal controls. Results The expression of p27 was lower in Bowen's disease and SCC compared to that in the normal skin, and the expression level decreased with a decline in the differentiation of tumors, i.e. the lowest expression was observed in poorly differentiated SCC, followed by highly differentiated SCC and Bowen's disease. The expression of cyclin E was higher in Bowen's disease and SCC than that in the normal skin, and the expression level increased with a decline in the differentiation of tumors. The expression of p27 was inversely correlated with that of cyclin E among all the cases of Bowen's disease and SCC. Conclusion The abnormal expression of p27 and cyclin E may contribute to the pathogenesis of Bowen's disease and SCC.