Brain ; physiopathology ; Brain Neoplasms ; surgery ; Cognition Disorders ; physiopathology ; Humans ; Magnetic Resonance Imaging ; methods ; Oxygen ; blood

OBJECTIVE: To probe into the cognition of the elderly to the safety of drug use and their demand for pharmaceutical care,and to improve pharmaceutical care in the hospital and guarantee the safety of drug use.METHODS: Questionnaires about medication information were designed.315 elderly patients,who were collected from 10 old-age homes of Liaocheng,domestic sickbed department and 5 community medical service stations of Liaocheng Municipal People's Hospital from Oct.2008 to Mar.2009,were involved in study.Investigation data were analyzed statistically using SPSS17.0 software.RESULTS & CONCLUSIONS: Results of study showed that the elderly had not sufficient knowledge towards the safety of drug use,especially drug allergies,side effects of drugs and special population medication.There was significant difference in the understanding of the safety of drug use among the elderly with different education levels,different occupations and different medical insurance patterns.The potential danger of medication is present.They are desirous of acquiring pharmaceutical care.Pharmacists should carry out medicine consultation and popularize pharmaceutical knowledge.They also should provide a comprehensive pharmaceutical care for the elderly people to ensure the safety of drug use in the elderly.

Immunoblotting(IB) technique has been frequently used to detect bullous pemphigoid (BP) antibodies in BP patient.It is important to extract the antigen proteins fully and intactly in the base membrane zone(BMZ) of normal human epidermis.The simple method of extracting antigens will make IB techniqne easy.Three different methods of extracting antigen proteins were compared.Using IB for detecting BP antibodies,it has been found that the results of epidermal extract preparations are basically similar.However,one of these methods (NO.1) is more convenient and may obtain more amounts of antigens.Another method(No.3) is identical to that of extracting antigens in dermis for de- tecting epidermolysis bullosa acquista (EBA) antibodies.Therefore,in using IB technique to distinguish BP from EBA,one can apply method No.3.

Apoptosis ; Child ; Cytokines ; immunology ; secretion ; Humans ; Systemic Inflammatory Response Syndrome ; immunology ; therapy

Objective To study the TPS dose distribution of the radiation therapy with wedge for upper-thoracic esophageal cancer.Methods Adopting spiral CT scanning,three dimensional data of the focus of infection and the other tissues were acquired.The radiotherapy plans with 45? wedges and without wedge were designed in TPS.The dose-volume histogram were calculated and compared.Results Although the average of dose of irradiated target was very close in the two plans,but there was remarkable difference in the dose uniformity.The Std(standard deviation) of dose in irradiated target was 6.31 in plan without wedge,and it was 4.45 in plan with the 45? wedge.The results show that the dose distri-butions of irradiated target are optimized by using the wedge.Conclusion It is a simple and effective method to use wedge for improving dose distribution in the radiation therapy for upper-thoracic esophageal cancer.

Objective To investigate the predictive value of ADC value in grading of neuroepithelial tumors. Methods The clinical data and images of 70 patients with neuroepithelial tumors pathologically proven were collected and analyzed restrospectively. All the patients were classified into low(WHOⅠ or Ⅱ)and high(WHO Ⅲ or Ⅳ)grade groups which included 40 and 30 cases respectively accroding to the 2007 WHO classification of tumours of the central nervous system. All the patients underwent plain and contrast-enhanced MR scan and DWI before surgery. The minimum ADC(MinADC)value was measured postoperatively on ADC maps. The Ki-67 labeling index(Ki-67 LI)of tumor tissue was determined by immunohistochemistry.MinADC values for two groups were analyzed using student t test, while the age and Ki-67 LI for the two groups was analyzed using Mann-Whitney test(P＜0.05 considered to be significant＝.Also the MinADC valHe and Ki-67 LI were analyzed with Pearson correlation. The receiver operating characteristic analysis was used for evaluation for grading neuroepithelial tumors of the patients using MinADC. Results The mean MinADC value[(1.08±0.31)×10~(-3) mm~2/s]of the low grade group was significantly higher than that[(0.74±0.18)×10~(-3) mm~2/s]of the high grade group(t=5.42,P＜0.05＝,and the Ki-67 LI of the low grade group[0-50%,median 4%]was significantly lower than that[0-75%,median 25%]of the high grade group(U=325.50,P＜0.05＝.There was a negative correlation between MinADC value and Ki-67 LI(r=-0.30,P＜0.05＝.The area under the ROC curve was 0.85,and the cutoff MinADC value of 0.86×10~(-3) mm~2/s for the differentiation between high and low grade neuroepithelial tumors provided the best combination of sensitivity(90.0%)and specificity(77.5%)(receiver operatingcharacteristic analysis).Conclusion MinADC value is helpful for prediction of neuroepithelial tumor grade.

As a new biotechnology, protein transduction connects the target fragment with biology active and protein transduction domain, and transducer it in to cells, exert its biological effects. Protein transduc-tion domain is a small molecule peptide, not only the protein transduction domain can penetrate through cell membrane, but could maintain the activity of its fusion protein. Protein transduction is an emerging biology technology, it has been applied into all aspects of cell gene regulation since its invention. In this article, we take Tat, Pdx-1 and Beta/NeuroD for instance and review the application of transduction protein in the dif-ferentiation of pancreatic stem cell.

Acute myeloid leukemia (AML) is the most commom hematologic malignancy in adults.There are so many highlights in the 54th ASH annual meeting in the field of mechanism,diagnosis and treatment of AML.Especially many studies have identified genes close related to AML development,clones related to relapse and molecular marker for prognosis using next-generation sequencing (NGS).Minimal residual disease (MRD) assay has been used for evaluation of therapy efficacy and posttherapy prognostication in AML treatment.The tyrosine kinase inhibitor targeting FLT3-ITD in this meeting is the best highlight in the treatment of refractory and relapsed AML.

Congenital adrenal hyperplasia (CAH) owing to steroid 21-hydroxylase deficiency (21-OHD) was a relatively frequent of autosomal recessive disorders characterized by the inactivation of the steroid-synthesizing enzyme in the adrenocortex.Corticosteroids (glucocorticoids and mineralocorticoid) replacement therapy was the primary treatment of 21-OHD.The main objective of 21-OHD treatment in children was to maintain normal growth.Inadequate or excessive treatment was commonly observed.A number of studies reported that 21-OHD adult were at increasing risk of developing metabolic syndrome and cardiovascular events.However,there was few researches on 21-OHD children with metabolic disorders,and no domestic reports.The article summarized recent clinical research progresses in research on the alterations of lipid and carbohydrate metabolism in children with classic 21-OHD.