Objective To explore the features of airway inflammation in patients with eosinophilic bronchitis(EB). Methods Sputum induced eosinophilic count was performed in 7 patients with EB(EB group), 12 patients with asthma(asthma group), 16 patients with cough variant asthma(CVA group), and 6 patients with atrophic rhinitis(AR group). The number of eosinophil and mastocyte was detected by bronchial biopsy in patients of EB and CVA group. Results The percentage of eosinophil counts of induced sputum in asthma group, CVA group, EB group and AR group was ( 18.7±5.3)%, ( 10.2± 4.5)% ,(8.7±2.3)% and (2.7±3.2 )% respectively. There were significant differences between EB group and the other three groups.The number of eosinophil in airway smooth muscle was significantly lower in EB group [ ( 1.3±0.1 )/mm2 ]than that in CVA group[ (5.2±1.2)/mm2]. And the mastocyte number in EB group[ (3.5±1.4)/mm2] was significantly lower than that in CVA group [ ( 12.5±2.4)/mm2]. Conclusions Perhaps EB is associated with many diseases. Aggressive treatment maybe prevent asthma. Eosinophil and mastocyte number is significantly low in patients with EB compared those with CVA, which maybe play an important role in absent airway hyper-responsiveness in EB.

Acetophenones ; pharmacology ; Caspase 3 ; metabolism ; Cell Proliferation ; drug effects ; Cells, Cultured ; Focal Adhesion Protein-Tyrosine Kinases ; metabolism ; Humans ; Muscle, Smooth, Vascular ; drug effects ; metabolism ; pathology ; Pulmonary Artery ; drug effects ; pathology

Cardiac involvement related to iron overload caused by long-term blood transfusion is a major cause of death for patients with thalassemia major. Patients' survival will be improved greatly if cardiomyopathy resulted from transfusional iron overload could be detected timely and effective iron chelation be initiated promptly. In the present article, various evaluation Methods currently used in clinical practice are reviewed, with particular focus on cardiac MRI for the monitoring of myocardial involvement related to iron overload.

Objective To explore the accuracy and the clinical significance about the diagnosis treatment to allergy rhinitis with bioresonanztherapie.Methods 22 cases who were confirmed as house dust mite,extremely sensitive to duse diagnosed by BICOM were randomly collected and examined by allergic-skin test,which were compared with the control group.Results 22 examples by BICOM bioresonanztherapie,for to the house dust mite and the dust highly sensitive patient,allergy test sets piercing experiment was the strong masculine genders.Conclusion Bioresonanztherapie is really feasible at accuracy and practicability to test allergic rhinitis.

Antigens, CD1 ; metabolism ; Child, Preschool ; Cholangitis, Sclerosing ; diagnosis ; etiology ; metabolism ; Diagnosis, Differential ; Female ; Histiocytosis, Langerhans-Cell ; complications ; metabolism ; pathology ; Humans ; Immunohistochemistry ; Liver ; metabolism ; pathology ; S100 Proteins ; metabolism

Objective To evaluate the feasibility of cell free fetal DNA(cffDNA)-based noninvasive prenatal diagnosis,we developed a precise technique for fetal sex determining region of Y chromosome(SRY)gene detection using size-fractionated cell-free DNA in maternal plasma.Methods Peripheral blood samples were collected form 117 pregnant women.cffDNA was extracted based on a column absorbent method and isolated by agarose gel electrophoresis.A dulex-polymerase chain reaction(PCR)was used to detected SRY gene and glycerol-dehyde-phosphate dehydrogenase(GAPDH)gene.Results Both SRY and GAPDH gene were detected in 86 cffDNA samples from women bearing male fetuses.And only GAPDH gene was detected in 71 cffDNA samples from women bearing female fetuses.These results had a coincidence whit those of villus or amniotic fluid samples.The specificity and sensitivity reached 100%(117/117)and 100%(66/66),respectively.Conclusion By agarose gel electrophoresis,re-extratedand and dulex PCR,size-fractionated cell-free fetal DNA in maternal plasma can be selective enriched and used to noninvasive prenatal diagnosis of sex-linked disorders and single gene disorders.

Choroidal metastasis is one of the most common malignant tumors inside the eyes. It causes pain, hypopsia and some other related symptoms. It reduces the quality of the patients' life. It's significant for the patients to be detected and treated early, therefore they will have better vision and longer life. The treatments of choroidal metastasis are developing quickly. Both the vitreous cavity injection of targeted drug and gene therapy are hot topics of research. This paper summarizes the etiology, development, diagnosis and treatment of choroidal metastasis nowadays.

? AIM: To analyze clinical characteristics and postoperative outcomes after open globe injury.?METHODS: Demographic characteristics ( age, gender, eye trauma, profession, cause of injury and injured part ) , as well as complications and prognosis were analyzed in 152 cases (152 eyes) of open globe injury.? RESULTS: Patients with open globe injury had an average age of 40. 45±38. 32 years old with a 5. 9:1 male-to-female gender ratio. The left-to-right eye ratio was 1. 27:1. Most patients were workers, farmers, or retired. The most common etiologies were scratches, boxing, and falls. Zone Ⅲ was the most commonly injured part. Iridoptosis or iris incarceration, retinal detachment, vitreal prolapse, hyphema or hypopyon, and vitreous hemorrhage were the most common complications. Visual acuity improved in 86 cases postoperatively but ophthalmectomy was still required in 25 eyes.?CONCLUSION: Vision can be improved after surgery in open globe injury. However, patients are usually seriously injured and improvement is minimal, thereby resulting in a great loss to patients and society.

Objective To study the effect of continuous positive airway pressure(CPAP) therapy for 56 clinical cases neonatal a-cute respiration prostration. Methods Based on the traditional treatment,CPAP assistant therapy were taken on 56 cases. The pressure of CAPA were adjusted according to the variations of clinical condition and blood gas value. Results After 6 - hour assistance of CPAP performance, the symptom of all cases had been abated, pa (O2) was increased, the normal CPAP applaying time was 28 hoours. The remarkable difference had been found compared to those in no CPAP practice(P

Objectives To evaluate the potential of antisense c myc gene therapy for acute promyelocytic leukemia by investigating the biological effects and molecular mechanisms in induction of differentiation of HL 60 cell line using recombinant antisense c myc adenovirus (Ad AS c myc). Methods Cultured HL 60 cells treated with Ad AS c myc or Ad LacZ with polybrene and protamine sulfate were analyzed by X gal staining, morphology, MTT, flow cytometric analysis, RT PCR, and immunocytochemical techniques in vitro . Results HL 60 cells could be transfected effectively by Ad LacZ+protamine sulfate (79.8%). The level of c myc transcription and the version could be strongly inhibited by Ad AS c myc in the transfected HL 60 cells. Ad AS c myc could strongly inhibit the cell growth in HL 60 cells (51%). Ad AS c myc could reduce the ratio of nuclear/cytoplasm, and increase the activity of peroxidase in HL 60 cells. Ad AS c myc could lead to the blocking of G 0/G 1 phase in HL 60 cells. Ad AS c myc could also increase the expression of c fos in HL 60 cells. Conclusion The expression of Ad AS c myc can inhibit the growth and induce differentiation of HL 60 cells in vitro . The biological effects of Ad AS c myc may be closely associated with the activity of peroxidase and c fos gene in HL 60 cells. Ad AS c myc is of clinical potential in gene thera py for acute promyelocytic leukemia.