Diabetes Mellitus ; diagnosis ; drug therapy ; Diagnosis, Differential ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Humans ; Hypoglycemic Agents ; therapeutic use ; Medicine, Chinese Traditional ; Phytotherapy ; Reference Standards

Anti-Arrhythmia Agents ; therapeutic use ; Arrhythmias, Cardiac ; drug therapy ; Child ; Humans ; Sotalol ; therapeutic use

Diabetes Mellitus ; drug therapy ; Diabetic Angiopathies ; drug therapy ; Diagnosis, Differential ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Humans ; Hypoglycemic Agents ; therapeutic use ; Medicine, Chinese Traditional ; Phytotherapy

Objective Three kinds of different internal promoters were inserted into the lentiviral vector to drive the expression of CD19-specifc chimeric antigen receptor (CAR)-redirected T lymphocytes with the green fluorescent protein(GFP) and the GFP expression efficiency of transduced cells driven by lentivirus were compared.Methods Three GFP reporter lentivirus vectors carrying different promoters and anti-CD19-CAR,including CMV,EF1α,and CMV-EF1α were selected.Three different vectors' names abbreviate to pHAGE-CMV-EFlα-EGFP-CD19,pHAGE-CMV-EGFP-CD19,pHAGE-EF1α-EGFP-CD19.Human embroic kideny 293T cells were cotransfected with the three plasmids by calcium phosphate DNA precipation.And the expression of GFP was observed under fluorescent microscope after transfecting 293T cells,and virus supernatant was collected after 72 h and centrifuged.Nucleic acid copy number in RNA and the expression of EGFP and CD3 zeta were identified through fluorescence quantitative PCR,flow cytometry and western blotting,respectively.The titers of the lentiviral vectors were determinde by scoring GFP expression following swerial dilutions of the viral supernatant on 293T cells.T lymphocytes was infected with lentivims produced from these reporter vectors.Then,fluorescence microscope,wesyern blotting were used to detect the GFP expression strength.Results Results of fluorescence microscope maintained that different internal promotors driving the expressing effect is different.293T as targeted cell,the transfected 293T cells were found containing strong expression of GFP.The amount of the 293T cells expressing GFP driven by CMV-EF1α promoter was the largest,while the EF1α promoter was the smallest.Flow cytometry results show that the rate of transduction is 72.4％,20.6％ and 14.5％,respectively.T lymphocytes as targeted cell,Western blot showed that CMV-EF1α was the largest among all promoters,while the CMV was the smallest.Conclusion In the study of gene expression mediated by lenuvirus,proper cell lines and promoters should be selected to obtain high efficiency.

Objective To observe the effects of early rehabilitation training on sensory restoration after the median nerve injuries. Methods Seventy patients with median nerve injuries were randomly divided into two groups: the rehabilitation group ( n =32), in which the patients received sensory reeducation two weeks after surgical treatment; and the control group ( n =38),in which the patients only received surgical treatment. After 6 8 weeks of treatment, reassessment were performed and the results were compared between the two groups. Results The excellent and effective rates of functional recovery in sensation in the rehabilitation group were 88% and 97%, respectively. A comparison showed that there was significant difference between the two groups. Conclusion Sensory reeducation could accelerate the restoration of sensation in patients with median nerve injury.

Fundamental specialized courses are important due to their connection between fundamental knowledge and major knowledge,which are regarded as the difficult courses for undergraduates to learn.The useful approaches and experience of bilingual teaching in microbiology,one of the fundamental specialized courses for the students majoring in Bioengineering,are presented here.The key aspects such as choosing textbooks,teaching manners and teaching contents are also discussed.This paper also proposes how to improve the quality of bilingual teaching during the fundamental specialized courses.

Objective: To develop an effective preparation method to improve the sensitivity and accuracy of real-time PCR in detection of BK virus's (BKV's) load in urine samples. Methods: A total of 24 samples documented as positive probes in primary detection were enrolled in this study. The candidate samples were prepared by 4 different approaches: unprocessed urine, BKV's DNA extracted from urine, 1:10 diluted urine, and 1:100 diluted urine; and then they were subjected to real-time PCR examination to obtain the viral load. The data obtained were analyzed by SPSS 11.0. Results: The four different preparation processes for urinary specimens had significant impact on detection results of real-time PCR. Three samples were negative in the unprocessed urine group and 66.7% of its samples had the lowest viral loads compared with the other three groups. Two samples in the 1:100 diluted urine group were negative and 79.2% of its samples had the highest viral loads, but its median load was similar to that of the 1:10 group. Viral gene was detected in all samples in the DNA extraction group and 1:10 diluted urine group, but the loss of the target gene was more severe in the DNA extraction group. Conclusion: The 1:10 diluted urine is better for real-time PCR detection of BKV's load, as it lose less viral gene and is more efficient, easy to perform and economical.

Alanine aminotransferase (ALT)abnormality is an indicator of the degree of liver inflammation caused by immune activation in patients with chronic hepatitis B (CHB).However,approximately half of the CHB patients with normal or mildly elevated ALT levels have concealed significant changes in hepatic histology.CHB patients with normal or mildly elevated ALT levels may have significant histopatho-logical changes in hepatic tissues,and those changes vary between HBeAg(+)and HBeAg(-)CHB patients.Attention and investigation on the clinical management of CHB patients with normal or mildly elevated ALT levels may have great significance in grasping the right treat-ment opportunity and reducing the risk of liver cirrhosis.

The present investigation was undertaken to evaluate the role of the Chinese fruit juice in blocking nitrosamine formation in vitro.A model system simulated the conditions (pH 3.3, 37℃) known, to exist in the stomach, was used in the experiments. Different amount of nitrite and aminopyrine was incubated with Chinese fruit juice or vitamin C solution or buffer solution for 1 hr. 0.1 ml samples were taken and then subjected to the Ames mutagenicity test. The tester strain TA100 of Salmonella typhi-murium was employed for the detection of mutagenicity due to the N-nitro-samine formed from sodium nitrite and aminopyrine. Test samples and bacteria with or without S-9 mixed with molten top agar and poured onto the minimal agar plates. After incubation, the sample, that gave at least a 2-fold increase in induced revertants, compared to spontaneous revertants, was considered to have mutagenic activity.It showed that the concentration of the precursors was at or above 5 mg/ ml with S-9 and without juice. the samples exhibited mutagenic activity. It suggested that the nitrosamine was formed in this system. While the incorporation of Chinese fruit juice was found to inhibit the formation of nitrosamine at 5mg/ml and 8mg/ml. The cultures did not show mutagenic activity. The induced revertant colonies per plate was 252 ? 4.2. The Chin esefruit juice is more effective than that of vitamin C, the induced revertant colonies per plate was 445 ? 81.2, which shows mutagenic. The difference between the cultures of Chinese fruit juice and vitamin C is significant (P

AIM: To study Cordyceps sinesis and its cultured mycelia by HPLC.METHODS: An HPLC established for the fingerprints of cordyceps sinensis and its cultured mycelia was performed on an Agilent C18(4.6 mm ? 25 mm,5 ?m)as column and acetonitrile-0.1% phosphoric acid solution in gradient as mobile phase.The flow rate was 0.5 mL/min,and the detection wavelength was set at 260 nm.RESULTS: There were 6 common peaks in HPLC fingerprint of Cordyceps corensis and its cultured mycelia,three of which were determined as uracil,adenosine,cordycepin.CONCLUSION: The method can be used for the study of fingerprints of cultured mycelia.