Objective:To explore the relationship between hypertension stage and blood pressure variability (BPV) in aged patients with hypertension ,and the relationship among target organ damage (TOD) ,age and BPV .Methods :A total of 180 aged inpatients with hypertension in our hospital received ambulatory blood pressure monitoring .Ac-cording to results of blood pressure monitoring ,patients were divided into hypertension stage 1 group (n=65) ,stage 2 group (n=61) and stage 3 group (n=54);according to their age ,they were divided into 60~70 years group (n=65) ,71~80 years group (n=68) and ≥81 years group (n=47) .BPV index was the measured standard deviation (SD) of blood pressure .Results:Along with hypertension stage rose (stage I~stage III) ,there was significant in-crease in BPV [24h mean BPV :(11.0 ± 2.5) mmHg vs .(12.2 ± 3.3) mmHg vs .(14.3 ± 3.6) mmHg , P<0.01 all] in aged patients with hypertension .For 24h systolic blood pressure (SBP) BPV /24h systolic blood pressure (DBP) BPV ,compared with those without TOD [(10.7 ± 2.3) / (8.0 ± 1.4) mmHg] ,there was significant rise in patients with heart damage [ (12.2 ± 2.4) / (9.8 ± 2.2) mmHg] ,brain damage [ (12.9 ± 3.1) / (9.6 ± 2.0) mm-Hg] and renal damage [ (15.7 ± 2.4) / (12.4 ± 1.5) mmHg]] , P< 0.05 or < 0.01. There was no significant difference in BPV among different age groups .Conclusion:Along with blood pressure rises ,blood pressure variabili-ty significantly increases in aged patients with hypertension ,and it's more significant in those with target organ dam-age .Relationship between blood pressure variability and age is not significant in these patients .

Immunoblotting(IB) technique has been frequently used to detect bullous pemphigoid (BP) antibodies in BP patient.It is important to extract the antigen proteins fully and intactly in the base membrane zone(BMZ) of normal human epidermis.The simple method of extracting antigens will make IB techniqne easy.Three different methods of extracting antigen proteins were compared.Using IB for detecting BP antibodies,it has been found that the results of epidermal extract preparations are basically similar.However,one of these methods (NO.1) is more convenient and may obtain more amounts of antigens.Another method(No.3) is identical to that of extracting antigens in dermis for de- tecting epidermolysis bullosa acquista (EBA) antibodies.Therefore,in using IB technique to distinguish BP from EBA,one can apply method No.3.

Adult ; Citric Acid ; adverse effects ; Dust ; Humans ; Male ; Middle Aged ; Occupational Exposure ; adverse effects ; Tooth Erosion ; chemically induced

Objective To investigate the expressions of CXC chemokine receptor 3 (CXCR3) and CD1a in skin lesions of different stages of mycosis fungoides (MF). Methods The expression and distribution profiles of CD1a and CXCR3 were detected by immunohistochemistry in the epidermis of skin samples from 16 normal human controls, 16 patients with non-tumor (patch/plaque) stage MF and 8 patients with tumor stage MF. Results With the progression of MF from patch/plaque stage to tumor stage, the positivity rate of CXCR3 in intraepithelial neoplastic cells in lesions dropped from 38.9% to 17.5% (P ＜ 0.05). The average number of intraepithelial Langerhans cells (LCs) per high power field (HPF) in skin samples was 10.6 in patch/plaque stage MF, significantly higher than that in normal control (7.3) and tumor stage MF (6.7), while no significant difference was found between normal control and tumor stage MF specimens. Conclusion The epidermotropism in MF may be associated with the expression of T-helper (Th) 1-associated chemokine receptor (CXCR3) and quantity of intraepithelial LCs.

The mutations and deletions of the MTS1/p16 gene are widespread in a variety of human tumor tissues.It has been shown that p16 plays an important role in the formation and development of cholangiocarcinoma as research on cholangiocarcinoma has progressed over the years, and it is thought that the abnormal expression of p16 in highly involved in cholangiocarcinoma-related diseases.All these findings indicate that p16 is closely related to cholangiocarcinoma.Early diagnosis of cholangiocarcinoma is still poor, so p16 may be a qualitative and quantitative tumor marker of high diagnostic value and may provide an opportunity for some new therapeutic methods such as targeted therapy.It can achieve the purpose of early diagnosis and early treatment to enhance the survival rate of patients.

jiaogulan ( Gynostemma pentaphyllum) extract (0.25~2g/L. final concentration) obviously inhibited the platelet aggregation & TXB2 release induced by arachidonic acid ( AA ) in vitro, and the ID50 of inhibiting TXB2 release was 0.28g/L. With the dose of 35mg/kg i. v. , it evidently inhibited platelet aggregation at 10 & 20 min after injection, and also markedly decreased TXB2 released by platelets during 10~40 min after injection, especialy within 20 min. Jiaogulan extract (0.25~4g/L) did not influence 6-keto-PGF1? release of aorta thoracalis of rabbits in vitro. Enzyme immunoassay was used to measure the content of TXB2 & 6-keto-PGF1? in the experiments. The above results suggest that Jiaogulan extract may decrease TXA2/PGI2 ratio.

Aim To establish the dementia mice models induced by intracerebroventricular infusion of ?-Amyloid peptide and related measuring index. Methods The mice models were made by intracerebroventricular infusion of long fragment of soluble?-Amyloid peptide with micropump, intracerebroventricular injection of long soluble?-Amyloid peptide fragment and short insoluble ?-Amyloid peptide fragment,and intracerebroventricular injection of ?-Amyloid peptide combining with transforming growth factor. Results Compared with normal control, the learning and memory ability was decreased in all above model mice. the ChAT activity in both hippocampus and cortex of model mice decreased, the hippocampal neuron were depleted and the content of apoptosis associated protein Bal-2?BAX and p53 were increased. Conclusions All above mice models could be choose as the dementia models which were simulating the pathological charactoristics of the Alzheimer's disease.

Purpose:To evaluate multiple biomarkers of colorectal tumor and their potential usage in automated diagnosis of colorectal cancer.Methods:10 normal colonic mucosae, 30 colorectal adenomas and 53 colorectal carcinomas with complete follow-up data were examined for multiple biomarkers(DNA contents, AgNOR, PCNA and nuclear morphometry) with quantitative computed processed imaging analysis.Results:The values of most of the parameters analyzed increased in accordance with the normal mucosa-adenoma-adenocarcinoma sequence, indicating a marked tendency for progression of colorectal malignancy. Among those parameters, DNA index,positive rates, counts, density and aspect factor of AgNOR, positive rates and density of PCNA, shape factor, width and density of nucleus were demonstrated to be relatively valuable indices.Conclusions:The quantitative evaluation of some indices of colorectal tumor can provide reproducible data for differential diagnostic of this tumor.

BACKGROUND: Mesenchymal stem cells from bone marrow stroma are able to differentiate into multiple mesoderm-type cell lineages. Because of the ease of their isolation and their extensive differentiated potentiality, mesenchymal stem cells become potential resource of cell and gene therapy. OBJECTIVE: To summarize biological characteristics and application of bone marrow mesenchymal stem cells. RETRIEVAL STRATEGY:An online search of Pubmed was undertaken by using the keywords of "Bone marrow mesenchymal stem cells, gene therapy, transplant"to identify the relevant articles published in English from January 1999 to December 2006. At the same time,Wanfang database was undertaken to identify the relevant articles published between January 1999 and December 2006 with the key words of "Bone marrow mesenchymal stem cells, gene therapy, transplant" in Chinese.The data were selected primarily, and then quotations of each article were checked. Inclusive criterion:The articles related to the biological characteristics and application of bone marrow mesenchymal stem cells were included. Exclusive criteria:the articles with repetitive research or Meta analysis were excluded.Totally 95 relevant articles were selected and 78 of them met the inclusive criterion. The 45 excluded articles were of old or repetitive content. LITERATURE EVALUATION: Selected articles were on basic experiment or clinical study of the biological characteristics, cell transplantation and gene therapy of bone marrow mesenchymal stem cells. Totally 33 articles of the 78 articles met the inclusive criterion were included. Of them, 4 articles were on the isolation of bone marrow mesenchymal stem cells, 3 on surface marker of bone marrow mesenchymal stem cells, 6 on biological characteristics of bone marrow mesenchymal stem cells, 3 on immune response between bone marrow mesenchymal stem cell transplantation and host and 17 on the application of bone marrow mesenchymal stem cells on disease therapy. DATA SYNTHESIS: In the suitable condition, bone marrow mesenchymal stem cells can differentiate into multiple cells, such as bone, chondrocytes, fat, muscle, tendon, neuron-like cells, myocardial cells and stroma-cells that support hematopoietic stem cells. Bone marrow mesenchymal stem cells seem to be the cells of immunosuppression and low-down immunogenicity. It makes bone marrow mesenchymal stem cells used in systemic transplantation, local implantation, as a vehicle for genes in gene therapy protocols or to generate transplantable tissues and organs in tissue engineering protocols. The results of these initial trials are very encouraging in animal test and several clinical trials are successful in some patients. But the enough clinical trails and long-term safety of therapeutics based on bone mesenchymal stem cells are needed. CONCLUSION: Biological characteristics of bone marrow mesenchymal stem cells have been gradually recognized. It is hoped that using bone marrow mesenchymal stem cells in clinic will bring major advances in the therapy of some diseases.

Objective The rapid latex scattering immune turbidimetric method detecting urine retinol binding protein, and evaluate methodology.MethodsIn accordance with the commission of American clinical laboratory standards (NCCLS) requirements, evaluation of the research methods of reagent sensitivity, precision, accuracy, stability, linear range, interference analysis, specific degrees.ResultsWith rapid latex scattering immune turbidity method to detect the urine retinol binding protein, for instance, the minimum detection limit of 0.0381 mg/L;Repeatability precision is 1.198%, intermediate precision is 5.541%, reproducibility precision is 6.662%;The linear range is within 0~10 mg/L;Recovery rate were 99.00% and 104.00% respectively;When TBIL<100mg/L and Hb<10mg/L, the detection of Tbil, Hb, RBP interference;With the urine RBP than kit in automatic analyzer compared the test results of analysis showed that r2=0.9720, correlation can be;50 cases of clinical specimens using two methods showed no statistically significant difference positive rate (χ2=0.948, P=0.948).ConclusionRapid latex scattering immune turbidimetric method were used to detect the urine retinol binding protein, has high precision, high accuracy, the advantages of high sensitivity, stability and anti-jamming is strong, good.