Objective To establish a numerical model of adsorption and desorption of CO_2 and to study the influencing factors on the removal system. Method A physical model was expressed by mathematical method, then an isothermal adsorption model for simulating molecular sieve was established. Result The working process of a CO_2 removed device was simulated and its result was analized with the established model.The characteristics of the adsorption and desorption was obtained. Conclusion The model describes the processes correctly, the influencing factors like humidity dimension of the bed are considered simultaneously.It remains to be improved by further experimental corrections.

Objective To apply the membrane technology for purifying rough cordyceps polysaccharides solution,the optimal operation condition and effective cleaning method were researched.MethodsTo mainly analyze the flux of membrane and its recovery.Results The optimal experiment operation condition obtained is 60℃,pH value 7-8,and pressure 0.3-0.4 MPa,by which the polysaccharides recovery was about 30%,more than by traditional ethanol extraction,and the most impurities as infusibility substance and protein colloid etc.were taken out.Moreover,the flux of membrane recovery can be above 90% after 80℃ water and 1% NaOH solution was alternately used to wash the fouling membrane under intermittent little back-pulsing. Conclusion The process of membrane technology purifying rough cordyceps polysaccharides is simpl and feasible.

Objective To observe the clinical efficacy of acupuncture plus rehabilitation training in improving post-stroke dysphagia. Method A total of 60 patients with post-stroke dysphagia were randomized into a treatment group and a control group, 30 cases in each group. The two groups were intervened by basic treatment of internal medicine and swallowing rehabilitation training, and the treatment group was additionally given acupuncture treatment. The Standardized Swallowing Assessment (SSA), water swallowing test and Swallowing Quality of Life Questionnaire (SWAL-QOL) were evaluated in the two groups before and after the treatment. Result After the treatment, the SSA scores in the two groups were significantly decreased compared to those before the treatment (P<0.01), and the SWAL-QOL scores were significantly increased compared to those before the treatment (P<0.01); the SSA score in the treatment group was significantly lower than that in the control group (P<0.01), and the SWAL-QOL score was significantly higher than that in the control group (P<0.05). The water swallowing test classification after the treatment was significantly superior to that before the treatment in the treatment group (P<0.01), and there was a significant difference compared to that in the control group (P<0.05). Conclusion Acupuncture plus rehabilitation training can obviously improve the swallowing function of patients with post-stroke dysphagia, and the clinical efficacy was superior to single rehabilitation training.

Tumor necrosis factor-related apoptosis-inducing ligand(TRAIL) is a new anti-tumor biological agents which is very hot in recent years through its death receptor-induced apoptosis of tumor cells,and non-toxic to normal cells,and has synergy with the chemotherapy drug,but there is also resistance mechanisms.This article will review the biological characteristics of TRAIL and its receptors,TRAIL-induced apoptosis of tumor cells,as well as the mechanism of TRAIL in leukemia in the progress of treatment.

Objective:To investigate the physical morphology of the root canal taper in human maxillary and mandibular permanent first molars.Methods: Forty maxillary and 44 mandibular extracted human permanent first molars were investigated.The roots were dissected transversely from their apical foramen vertical to the long axis of the root,and 1-mm-thick serial sections were made.The root apex side of each section was observed with a stereomicroscope at 30? magnification.The maximum and minimum canal diameters were measured at 0,1,2,and 3 mm from the apical foramen.The physical canal taper in the apical section was calculated.Results: 47.5%-60.0% of the narrowest diameter of root canals were present at the foramen,and 25.0%~42.5% located at the level of 1 mm from apical foramen.Almost all physical configurations of root canals in 3 mm apical region showed a bottleneck-like taper.Canal dia-meters increased rapidly at 1-2 mm with the biggest taper,and the taper had a bigger variance(0.07-0.35).The ring of shrunken bottleneck was formed at the level of 1 mm from apical foramen.The taper of canal in maximal diameter was larger than the one in minimal diameter.Conclusion: The physical morphology of bottleneck-like taper in apical canal was helpful to locate the canal apical stop in root canal treatment.

Objective To establish an HPLC method for determination of the content of paeoniflorin in in Anwei Granules.Methods With Phonomenex C18,150mm?4.60mm,5micron,acetonitrile-water-phosphoric acid(15∶85∶0.2)was used as the mobile phase,The flow rate was 1.0ml/min.The detection wavelength was 230nm.Results The linearity was obtained from 2.52-20.14 ?g(r=0.9990).The average recovery was 99.17 % with RSD=0.91%(n=6).Conclusions The method is sensitive,simple,specific and accurate for determination of astragaloside in Tangshenkang Granules.

Conjunctival Impression Cytology (CIC) method was modified to assess vitamin A level more exactly and simply in China. The infants (

Objective To investigate the expressions of SOCS3 and IL‐6 in triple‐negative breast cancer(TNBC)and their clinical significance.Methods The expressions of SOCS3 and IL‐6 in TNBC and normal breast cells were detected by Western blot and fluorescence quantitative PCR.Immunohistochemical staining was used to detect their expressions in 84 TNBCs ,33 breast fibromas and 11 normal breast tissues. The correlation of their expressions in TNBC and the relationship between their expressions and clinical pathological parameters were analyzed as well.Results SOCS3 was not expressed or down‐expressed in TNBC cell lines while IL‐6 was over‐expressed. The positive rate of SOCS3 protein was 32.1% in TNBCs ,63.6% in breast fi‐bromas(P<0.01)and 72.7% in normal breast tissues (P< 0.01);and that of IL‐6 protein was 72.6% in TNBCs ,30.3% in breast fibromas(P< 0.01)and 27.3% in normal breast tissues (P< 0.01).Moreover ,a significant negative correlation was found between the expression of SOCS3 and that of IL‐6 in TNBC(rs = -0.590 ,P<0.01). The expressions of SOCS3 and IL‐6 were markedly associated with tumor size ,lymph node metastasis ,clinical stages and expressions of Ki‐67 and p53(P<0.05) , but not with age ,histological grades or menopausal status(P>0.05).Conclusion The expressions of SOCS3 and IL‐6 are asso‐ciated with the development of TNBC ,suggesting that SOCS3 and IL‐6 can serve as biological markers for evaluating the prog‐nosis ,and therapeutic targets of T NBC.

OBJECTIVE:To investigate the effect of extract of Ginkgo biloba(EGb) on levels of Endothelin-1(ET-1) and transforming growth factor ?1(TGF-?1) in renal tissue of diabetic rats.METHODS:Twenty-four male Sprague-Dawley rats were randomly divided into three groups:normal control group,diabetic model group and EGb-treated group.The levels of blood glucose,insulin,total cholesterol(TC),total triglycerides(TG),creatinine clearance(Ccr),urinary albumin excretion(UAE),and urinary ?2-MG were measured after 8-week corresponding treatment.Expression of SET-1,UET-1,and RET-1 was examined by radioimmunoassay technique.Expression of STGF-?1 and UTGF-?1 in serum and urine was examined by enzyme linked immunosorbent assay(ELISA).RESULTS:The concentrations of blood glucose,blood insulin,TC and TG increased significantly in diabetic group,which were down-regulated by EGb.Levels of ET-1 and TGF-?1.in both blood and urine and the ET-1 level in renal tissues were significantly higher in diabetic model group than in normal control group(P

Objective: To establish a peptide nucleic acid-mediated one-step PCR assay for detecting K-ras mutation, and to evaluate its diagnostic value. Methods: We developed a one-step polymerase chain reaction (PCR) approach with melting curve analysis using wild-type specific peptide nucleic acid (PNA) and fluorescent dye SYBR Green I to determine the genotypes in codon 12 and 13 of K-ras oncogene. The result of our method was compared with that of restriction fragment length polymorphism (RFLP) analysis. Results: Our method simultaneously examined condon 12 and 13 of K-ras oncogene, and was easy to perform. The sensitivity of our method was 0.001% when in a 105-fold excess of wild-type K-ras DNA. The detection limit was 102 copies. Our method could be used for large sample test, with the time of a single test being 1 hour. The limit of traditional method was 103 copies and the sensitivity was 0.01%. The testing time was about 2 days for samples which needed only 1 hour using our method. Conclusion: Our method has obvious advantage over RFLP analysis; it is suitable for detecting K-ras mutation in large samples and can be used as an effective method for early diagnosis of pancreatic cancer.