ObjectiveTo investigate the effects of recombinant human erythropoietin (rhEPO) on the apoptosis of human mesenchymal stem cells induced by H2O2 and related cell signaling pathways.Methods After being cultured in vitro, human MSCs were treated with rhEPO.Phosphorylation of ERK1/2,p38 MAPK and PI3K/Akt was detected by using Western blotting.One h after pretreatment with1U/ml rhEPO,cells were cultured in the presence of1mmol/L H2O2 for1h.Cell morphology was observed under the inverted microscopy.Cell apoptosis was determined by using flow cytometry,migration assay was performed in Transwell chambers,and adhesion assay was performed by plastic dishes.ResultsRhEPO could increase phosphorylation of PI3K/Akt pathway in human MSCs,but reduce phosphorylation of p38MAPK.RhEPO had no obvious effect on ERK1/2 pathway and total proteins of Akt and p38MAPK.RhEPO could decrease apoptosis of human MSCs induced by H2O2 (P＜0.01) and the inhibitory effect was abrogated by Ly294002 but not anisomysin.Conclusion RhEPO could protect MSCs from apoptosis. Activation of PI3K/Akt pathway was involved in the effect of rhEPO on apoptosis.

Superantigens are potent activators of T cells,causing rapid and massive proliferation of T cells and superantigen-dependent-cell-mediated cytotoxicity with extremely low doses.However such antitumor effects lack selectivity.Thus with the methods including monoclonal antibody targeting or binding superantigens to the surface of tumor cells as well as genetic engineering,scientists have done lots of work on targeted therapy of malignancies with superantigen.

The brainstems of 6 rabbits were cut transversely in series and stained fornerve cells or myelin.The architecture of the parabrachial nucleus was studied.Be-sides general observation the length and width of 100 nerve cells in each of the 5nuclear areas chosen were measured and analysed statistically with the help of adigital computer.The following items were calculated:ratio of length to width,itsmean and 95% tolerance interval;area of nerve cells,its mean and 95% toleranceinterval;80% elliptical contour of normal bivariates for the length and width of thecells;cluster analysis of the more dispersed nuclear areas and 80% elliptical contourof each cluster. We concluded that the parabrachial complex consists mainly of 3 nuclei,viz.medial parabrachial nucleus,lateral parabrachial nucleus and magnocellularparabrachial nucleus.Dorsal to the medial parabrachial nucleus there is asmall area,which was tentatively called“d”area in the present study.The medial parabrachial nucleus lies medial to the brachium conjunctivum.Its cells are more or less round in shape,varying markedly in size and canbe clustered into 3 groups according to their size.The lateral parabrachial nucleusis located lateral to the brachium conjunctivum.Its cells are smaller than those ofthe medial nucleus,mostly spindle in shape.Some larger cells can also be seen scat-tered among the smaller ones and the process of clustering has yielded 2 groups ofcells.The magnocellular parabrachial nucleus can be seen in the middle range ofthe nuclear complex.It lies ventrolateral to the brachium conjunctivum with denselypacked large round cells.Its demarkation with the surroundings is sharp.A dorsalextension of this nucleus can be seen wedging between the brachium conjunctivumand the lateral parabrachial nucleus.Its cells are also large and compacted but arespindle in shape.Cells of the“d”area are small and round.Its relation with eitherthe medial or the lateral parabrachial nucleus remain unsettled.The relation between the parabrachial nucleus and the K(?)lliker-Fuse nucleuswas discussed.

Objective: To give full play to the basic role of medical equipment to offer advice and suggestions. Methods: the primary health institutions medical equipment in the quality management and technology security problems, analyze the cause of the problems and suggestions. Results: the basic medical service platform to create high quality excellent, the establishment of a virtuous circle of medical service. Conclusion:we should pay attention to a balanced allocation of resource of high grade medical treatment, pay attention to primary health institutions medical service platform construction.

Objective: To reveal if the use of antimicrobi als is rational in the authors hospital.　Methods: 1 025 cases were investigated retrospectively by systematic sampling from all the inp atients of the year 1997.　Results: 77.8% of the subjects were treated with antimicrobials, of whom, 55.2% treated with 2 or more antimicr obials. Yet only 39 cases had microbiological test before the administration of antimicrobials.　Conclusion: Inappropriate use of antimicr obials exists in the hospital. The management of the use of antimicrobials and c ontinuing education of medical doctors should be enhanced so as to make the use of antimicrobials more rational.

Objective To explore the clinical significance of osteopontin (OPN) in systemic lupus erythematosus (SLE) and its pathogenic role in SLE by studying the correlation between OPN and clinical manifestations,laboratory parameters and disease activity.Methods The enzyme-linked immunosorbent assay(ELISA)was used to measure the level of OPN in serum of 68 SLE patients and 20 healthy controls.the clin-ical data and laboratory parameters were recorded.Results The positive rate of OPN was 79.41%in 68 SLEpatients and could not be detected in healthy controls.Compared with healthy controls.the positive rate and level of OPN in SLE patients was significantly higher(P＜0.01).There was no significant difference in age.gender and disease duration between OPN positive and negative lupus patients(P＞0.05).The Drevalence of fever,hair loss,WBC decrease,liver damage,serum C4 decrease,proteinuria and anti-dsDNA antibody in OPN positive SLE were significantly higher than those of OPN negative SLE(P＜0.05).The positive rate of serum OPN in SLE patients was significantly higher than that of anti-dsDNA antibody and anti-Sm antibody (P＜0.01).The level of plasma OPN was associated with systemic lupus erythematosus disease activity index (SLEDAI)(r=0.292,P＜0.05).The positive rate and level of OPN in active SLE patients was significantly higher than that of inactive SLE patients(P＜O.05).Conclusion The level of plasma OPN has a close rela-tionship with the activity of SLE.It may serve as an active disease marker of SLE.

Previous work showed that there was high ratio of Mycoplasma hyorhinis infection in human gastric carcinoma. P37 protein is a membrane lipoprotein of Mycoplasma hyorhinis. It was reported that P37 inhibited cell adhesion and induced tumor invasiveness. To investigate the function of P37 in cancer development, the p37 gene was subcloned into shuttle vector of pAdTrack-CMV. Linearized pAdTrack-CMV-p37 was co-transformed into BJ5183 cells with adenoviral genomic plasmid pAdEasy-1. The identified recombinant DNA was transfected into 293 cells to package adenovirus. From the supernatant and cell lysis, the presence of recombinant adenovirus P37 was proved by PCR. And then BICR cells with this recombinant adenovirus of P37 were successfully infected. RT-PCR and Western blot demonstrated the transcription and expression of P37 in infected BICR cells. And the soluble P37 protein can be secreted into the culture supernatant of infected BICR cells. In the migration assay in vitro, the number of migration BICR cells infected with Ad-p37 was 52.03% more than that of control. The construction of recombinant adenovirus provided a good tool for studying P37 function and its molecular mechanism, which will be further used for in vivo migration and invasion experiments.

OBJECTIVE To investigate the degree of risk of viral prevalence of HBV,HCV and HIV through blood transfusion in Beijing Hospital in China,and to assess the need of a national Haemovigilance System. METHODS Retrospectively,7883 blood bank specimens (collected from 2004 to 2007) were re-examined using 8 indicators (including 5-item Hepatitis B,anti-HCV,anti-HIV and Syphilis) for the prevalence of most common viral infection. RESULTS From the blood bank specimens,the prevalence of HBsAg was 0.88% (69),the anti-HBc positive blood,only the anti-HBc was found in 2.65% of the specimens,while both the anti-HBc and the anti-HBe were found in 2.09% of the specimens. The prevalence of anti-HCV was 0.09% (7). CONCLUSIONS We need to establish the national Haemovigilance System to strengthen the monitoring of the above HbsAg,HBcAb and HCV indicators to prevent the transfusion-transmitted infection. Only in this way can the public confidence in blood safety be improved.

Objective To design a kind of mobile doctor-nurse workstation in order to adapt to medical logistic information construction and improve the ability of medical treatment team under field condition. Methods With using the combination technology such as wireless network, communication, barcode and computer database, the mobile workstation based on 2D barcode at last was developed. Results The workstation can make the field medical treatment team information system towards wireless network and informationization more well and provide the first-hand data for performing treatment and medical care under field condition. Conclusion The application shows that the workstation can be well adapted to the requirement of field condition with quick and high performance characteristic, in addition, the system is convenient, stable and has friendly interface.

Objective To provide the basis for determining the suitable collecting time of safflower. Methods To measure the contents of saflor yellow-A in safflower crude drug from different collecting time in one day by HPLC. Results Only saflor yellow-A has big change. By experimental analyzing samples of different year and region, A bright time collecting in the flok, the content of saflor yellow-A in medicine assured higher. Conclusion A bright time collecting has some scientific basis.