Laser-induced breakdown spectroscopy has become a very attractive technique in the field of chemical analysis.The technique utilizes a laser focused on a small spot to create a micro-plasma on the sample surface.In recent years, laser-induced breakdown spectroscopy (LIBS) is a frontier analysis technique in spectrometric analysis, with the advantages such as real-time, online and non-contact analysis.The research and development of various instruments based on this technology has attracted great attention of researchers.In the article, we reviewed the research progress of LIBS instrument from the components and field equipment in recent years, mainly including portable LIBS, handheld LIBS and remote LIBS.

BACKGROUND:Currently a big controversy erupts in the repair of abdominal wal defects with composite patch. OBJECTIVE:To compare the composite patch and biological patch in the repair of abdominal wal defects in dogs. METHODS:Ten dogs were selected to prepare abdominal wal defect models, and then randomized into experimental group subjected to composite patch repair and control group subjected to biological patch repair. Incision adhesion was assessed at postoperative 30, 60, 90 days; patches and defect tissues were taken and detected histologicaly at postoperative 14 days. RESULTS AND CONCLUSION:The incision adhesion in the experimental group was milder than that in the control group at different time post operation (P < 0.05). At 14 days post operation, the material structure was clear with no signs of absorption in the experimental group, and there were a large amount of infiltrated inflammatory cels around the patch material, such as neutrophils and lymphocytes, and non-phagocytic patch was found; in the control group, the material structure was fuzzy with the presence of inflammatory cel infiltration and slight signs of absorption. Moreover, inflammation and fibrosis were severer in the control group than the experimental group. These findings indicate that compared with the biological patch, the composite patch is better to effectively reduce inflammatory reactions.

Despite the immense achievement in the field of IVF in recent years, many patients still suffer from recurrent implantation failure. Therefore, much attention has been drawn to its etiology and treatment. Chromosomal abnormality, sperm DNA damage, zona hardening, and inappropriate culture conditions are important factors that lead to recurrent implantation failure. Results of studies suggest that preimplantation genetic screening does not improve the rate of implantation or live birth. Comparative genomic hybridization array and single nucleotide polymorphism array could offer a more comprehensive screening of chromosomes. Assisted hatching may help to solve the problem of zona hardening in some situations. Co-culture and blastocyst transfer could be conducive to the improvement of the rates of implantation and pregnancy. Cytoplasmic transfer may give a solution to ooplasmic composition anomalies.
Chromosome Aberrations ; Coculture Techniques ; Embryo Implantation ; Embryo Transfer ; Female ; Fertilization in Vitro ; Germ Cells ; Humans ; Male ; Pregnancy ; Preimplantation Diagnosis ; Treatment Failure

Medicine, Chinese Traditional ; Reproductive Techniques, Assisted

[Objective]Discussion on TCM name and pathogenesis of hypertension. [Method]To further explore and analyze the TCM name, location as wel as pathogenssis of hypertension after learning“Neijing”. [Result]Hypertension corresponding TCM diseases should be the veins bulge from“Neijing”. The location of the disease is heart,while it relates to internal organs. The main pathogenesis is that heart commanding blood abnormality. [Conclusion ]The traditional classical dialectical mode from the liver treatment of hypertension should be changed,the clinical treatment of hypertension is due to the heart according to the theory of pathogenesis which the heart commands blood vessels.Seeking the key reason is important to differential treatment of hypertension.

Objective To explore the effect of early neuromuscular electrical stimulation (NMES) combined with sour taste therapy on dysphagia in the oral phase of acute/subacute ischemic stroke patients. Methods From January, 2013 to June, 2015, 90 patients with oral phase dysphagia after stroke were randomly assigned into NMES group (NM group), sour taste group (ST group ) and NMES combined with sour taste group (TO group) with 30 patients in each group. The swallowing function was evaluated with Functional Oral Intake Scale (FOIS) at baseline, three weeks and twelve weeks treatment. Results The change of FOIS was better in TO group than in ST group three weeks after treatment (P<0.01), and was better in TO group than in ST group and NM group twelve weeks after treatment (P<0.05). Conclu-sion An early application of NMES combined with sour taste therapy could improve the swallowing coordination, and reduce the the poten-tial complications in oral phase of acute/subacute ischemic stroke patients with dysphagia.

Objective To explore the method and performance of using multiple indices to diagnose sepsis and to predict the prognosis of severe ill patients.Methods Critically ill patients at first admission to intensive care unit (ICU) of Changzheng Hospital, Second Military Medical University, from January 2014 to September 2015 were enrolled if the following conditions were satisfied: ① patients were 18-75 years old;② the length of ICU stay was more than 24 hours; ③ All records of the patients were available. Data of the patients was collected by searching the electronic medical record system. Logistic regression model was formulated to create the new combined predictive indicator and the receiver operating characteristic (ROC) curve for the new predictive indicator was built. The area under the ROC curve (AUC) for both the new indicator and original ones were compared. The optimal cut-off point was obtained where the Youden index reached the maximum value. Diagnostic parameters such as sensitivity, specificity and predictive accuracy were also calculated for comparison. Finally, individual values were substituted into the equation to test the performance in predicting clinical outcomes.Results A total of 362 patients (218 males and 144 females) were enrolled in our study and 66 patients died. The average age was (48.3±19.3) years old. ① For the predictive model only containing categorical covariants [including procalcitonin (PCT), lipopolysaccharide (LPS), infection, white blood cells count (WBC) and fever], increased PCT, increased WBC and fever were demonstrated to be independent risk factors for sepsis in the logistic equation. The AUC for the new combined predictive indicator was higher than that of any other indictor, including PCT, LPS, infection, WBC and fever (0.930 vs. 0.661, 0.503, 0.570, 0.837, 0.800). The optimal cut-off value for the new combined predictive indicator was 0.518. Using the new indicator to diagnose sepsis, the sensitivity, specificity and diagnostic accuracy rate were 78.00%, 93.36% and 87.47%, respectively. One patient was randomly selected, and the clinical data was substituted into the probability equation for prediction. The calculated value was 0.015, which was less than the cut-off value (0.518), indicating that the prognosis was non-sepsis at an accuracy of 87.47%. ② For the predictive model only containing continuous covariants, the logistic model which combined acute physiology and chronic health evaluation Ⅱ (APACHE Ⅱ) score and sequential organ failure assessment (SOFA) score to predict in-hospital death events, both APACHE Ⅱ score and SOFA score were independent risk factors for death. The AUC for the new predictive indicator was higher than that of APACHE Ⅱ score and SOFA score (0.834 vs. 0.812, 0.813). The optimal cut-off value for the new combined predictive indicator in predicting in-hospital death events was 0.236, and the corresponding sensitivity, specificity and diagnostic accuracy for the combined predictive indicator were 73.12%, 76.51% and 75.70%, respectively. One patient was randomly selected, and the APACHE Ⅱscore and SOFA score was substituted into the probability equation for prediction. The calculated value was 0.570, which was higher than the cut-off value (0.236), indicating that the death prognosis at an accuracy of 75.70%.Conclusion The combined predictive indicator, which is formulated by logistic regression models, is superior toany single indicator in predicting sepsis or in-hospital death events.

Objective To evaluate the clinical value of CT three-dimensional(3-D) imaging on diagnosing of bone and joint diseases.Methods The imaging findings of 357 cases proved by operation or clinic were analyzed retrospectively by the 3-D imaging.The diagnosis accuracy of 3-D imaging was compared with that of routine CT.3-D images were obtained by surface shade display(SSD),multi-planar reconstruction(MPR),maximum intensity project(MIP) and volume rendering(VR).Results In 357 patients,257 cases were traumatic disease,100 cases were non-traumatic disease.The diagnosis accuracy of 3-D imaging was 98.3%,which was higher than that of routine CT(P

Objective To explore whether there is any abnormality in the tongue position of the open bite. Methods Tongue position was defined as the relative position of its body in the oral proper cavity on profile cephalometric roentgenogram which was enhanced by the iodine oil applied to the tongue surface. The tongue position was compared between open bite group and normal bite group with student's t test to determine the variation of open bite. Results Compared to the normal bite group, the root of tongue was closer to the uvula in the open bite group, and the distance between the dorsum of tongue and the surface of palate was decreased as well as the tip of tongue was nearer to the incisors. Conclusion The open bite's tongue position is characterized by more upward and more forward and this position may be one of the causes of open bite.

Adult ; Air Pollutants, Occupational ; analysis ; poisoning ; Arsenic Poisoning ; complications ; Causality ; Female ; Humans ; Male ; Maximum Allowable Concentration ; Purpura, Thrombotic Thrombocytopenic ; epidemiology ; Young Adult