OBJECTIVE:To observe the protective effect of Zhuyu annao pill on model rats against cerebral hemorrhage. METHODS:The experimental rats were randomized to 6 groups:blank group,model group,Angong liuhuang pill group,Zhuyu annao pill high,medium and low dosage groups. Cephalophyma model of rats were duplicated with rats' autoblood clot; the neurologic impairment change was recorded by integration method; the water content of the brain tissue was measured by dry-wet specific density method; meanwhile,the changes in hemorheology,blood coagulation (4 parameters),the blood coagulation time and the histology of the morphology of brain tissue were observed. RESULTS:In rats treated with Zhuyu annao pill (high,medium or low dosage) as compared with model group,no significant change was noted in the water content and neurological impairment; however,the pro-time (PT) was significantly prolonged(P

Objective: To analyze the problems of large medical equipment allocation in our country, put forward the method and basis of scientific configuration. Methods: The current situation of large medical equipment allocation was analyzed by the methods of literature, statistics and analysis, in order to understand the operating costs and benefits and provide a scientific configuration tool when bringing in medical technology. Results: The configuration of large medical equipment has some problems, such as the uneven distribution of medical resources, equipment shortage, blind purchase of high-end equipment, low localization rate and so on. These problems will directly lead to the unreasonable allocation and the waste of medical resources. Conclusion:The configuration of large medical equipment must comply with the relevant provisions of the state by the HTA and other scientific methods, so as to improve the level of investment decision-making. It will make the use of medical resources efficiently and reasonably.

Chemotherapy with or without molecule-targeting agents is the primary choice for patients with unresectable metastatic coloreetal cancer. New treatment strategies like intermittent therapy and maintenance therapy may not inferior to treatment until progression or unacceptable toxicity in maintaining the benefit for patients who responded or had stable disease after the first-line therapy,and also can reduce the drug related side effeets and improve the quality of life.

For the children,viral encephalitis is a common infectious disease of central nervous system,which is characterized by high incidences,unfavourable prognosis and high mortality rate.The Japanese encephalitis virus,herpes simplex virus,enteroviruses,EB virus,cytomegalovirus are the common pathogens.There are some emerging virus also cause encephalitis,such as west nile virus,borna disease virus,banna virus,nipah virus and so on.It is of significance for the doctors and scientific research personnels to study the pathogens of viral encephalitis.

Objective To investigate the importance and method of early treatment of pelvic fracture combine with hemorrhagic shock.Methods Review the experience comes from emergent treatment of 74 cases of pelvic fracture combine with hemorrhagic shock in the department of emergent surgery.To study the characteristics,meaningful therapy and the affects to course of pelvic fracture.Results By effective early anti-shock treatment.Conclusion All 74 patients of pelvic fracture combine with hemorrhagic shock were safety recover from shock phase and get further treatment.

Objective: To establish a rapid approach to the detection and identification of Mycobacteria from lesions of patients with suspected Mycobacterial infections. Methods: Specimens were obtained from five patients suspected to have Mycobacterial infections. DNA extracted from clinical samples was amplified by nested PCR. The PCR products were digested with Hha Ⅰ, MboⅠ, and BstUⅠ restriction enzymes and applied to PAGE. The species of Mycobacteria were determined by restriction fragment length polymorphism (RFLP) analysis. Identification of Mycobacteria culture was also performed in 3 patients. Results: M. marinum was found in two patients diagnosed as swimming pool granuloma. M. tuberculosis was found in one patient diagnosed as infectious skin granuloma. All these 3 Mycobacteria were confirmed by Mycobacteria culture. A strain of M. tuberculosis and a strain of M. fortuitum were detected in remain two patients. Conclusion: The results above indicate that PCR-RFLP analysis is rapid and reliable in detection and identification of different Mycobacteria species from skin tissues. Application of this method will be helpful for early diagnosis and treatment of Mycobacteria skin infections.

[Objective]To study the proliferation inhibition effect of decetaxel combined with cisplatin on osteosarcoma cells,and the effect of the drugs during the course of chemotherapy for osteosarcoma.[Method]The effect of docetaxel and cisplatin alone or combined with osteosarcoma cell line HOS-8603 was examined by cell count,morphologic observation,flow cytometry(FCM).The animal model of human osteosarcoma was established in nude mice.They were given docetaxel or cisplatin alone and combined,and a blank control group was set up.Observations on the growth and pathologic markers of osteosarcoma were carried out and pathological examinations were made.[Result]Docetaxel and cisplatin either alone or combined inhibited significantlythe proliferation of osteosarcoma cells and induced obviously apoptosis,and the effect of the drags combined was more effective than that of the drugs alone,Growth inhibition ratio ≥59.34%(P

Objective To study the effect of transmembrane tumor necrosis factor-alpha (TM-TNF-αt) on human cervical carcinoma HeLa cells,and explore the correlations with silencer of death domain (SODD).Methods The expression of proliferating cell nuclear antigen (PCNA) was detected by immunocytochemistry.The expression of SODD in human cervical carcinoma HeLa cells undisposed and after disposed with TM-TNF-α was detected by reverse transcription-polymerase chain reaction,and the influence of TM-TNF-α on human cervical carcinoma HeLa cells was analyzed.Results The positive rate of PCNA in undisposed human cervical carcinoma HeLa cells was 80.3％ (155/193),in human cervical carcinoma HeLa cells disposed with TM-TNF-α was 46.7％ (85/182),there was statistical difference (P ＜ 0.05).The cycle index of polymerase chain reaction in human cervical carcinoma HeLa cells was 28 times,the amplification product was disposed by 2％ agarose gel electrophoresis,the gray scale disposed by TM-TNF-α and undisposed human cervical carcinoma HeLa cells were 1.377 ± 0.170 and 0.815 ± 0.040,there was statistical difference (P ＜ 0.05).Conclusion TM-TNF-α has obvious cytotoxic effect on human cervical carcinoma HeLa cells in vitro which may due to its up-regulating the expression of SODD.

Objective To summarize and analyze the failure reason of internal fixation operation in peritrochanteric fractures and propose appropriate prevention measures.Methods The clinical data of 229 patients with peritrochanteric fractures were retrospectively analyzed,the failure reason of internal fixation was analyzed by the univariate and multivariate Logistic regression analysis.Results The univariate analysis showed that the patients' age,gender composition,types of internal fixation had no significantly correlated with the failure of internal fixation (P ＞ 0.05) ; the Garden reset index,bone quality,fracture type and load time had significantly correlated with the failure of internal fixation (P ＜ 0.05).The multivariate Logistic analysis showed that load time was the independent risk factors for fixation failure (OR =13.515).Conclusion The weight-bearing time points should be selected according to the bone quality,fracture type,and degree of intraoperative reset,thereby reducing the risk of surgery failure.

Objective To investigate the possible role of mycobacterial infection in the pathogenesis of sarcoidosis.Methods DNA fragments of mycobacterial16S rRNA gene and the complex-specific insertion sequence IS6110of M.tuberculosis were detected by polymerase chain reaction(PCR)in formalin-fixed,paraffin-embeded skin biopsy specimens from12patients with sarcoidosis.Results Mycobacterial common sequence16S rRNA gene was found in6patients,among which5were further confirmed as M.tuberculosis,the other one was atypical mycobacteria.The IS6110DNA fragment amplified by PCR was confirmed by DNA sequencing.Conclusion The above findings suggest an aetiological role of mycobacterial infection,especially M.tuberculosis,in some patients with sarcoidosis.