OBJECTIVE:To investigate the protective effect of trimetazidine against myocarditis induced by coxsackie virus B3(CVB3)in mice.METHODS:Myocarditis in Balb/c mice was induced by CVB3.Then the mice were randomized to blank group,myocarditis control group and trimetazidine(low,medium,and high concentrations)treatment groups for 7 days.Serum levels of IFN-?,TNF-?,IL-10 and IL-12 were determined on mice,meanwhile the virus titer of the myocardium, pathologic change,MDA concentration,SOD and CAT activities were detected.RESULTS:As compared with myocarditis control group,in groups treated with trimetazidine,serum levels of IFN-?,IL-10 and IL-12 increased significantly, but the change in serum TNF-?level was nonsignificant,the virus titer of myocardium decreased and pathological lesions of myocardium were ameliorated,the MDA concentration decreased,but SOD and CAT activities were up-regulated (P

18F-fluorodeoxyglucos (18F-FDG) PET/CT can provide metabolic information for three dimensional conformal radiation therapy planning. The biological target volume may be influenced by many factors. Studies of target delineation using 18F-FDG PET/CT and methods to improve the misalignment of PET and CT images due to breathing were reviewed in this article.

Objective To observe the effects of Lipopolysaccharide (LPS) in different concentrations on expression of Robo4 in human umbilical vein endothelial cells (HUVECs). Methods HUVECs were cultured in vitro and randomly divided into 3 groups: control group, low dose LPS group (10 μg/mL) and high dose LPS group (100 μg/mL). Robo4 protein level was detected by immunofluorescence staining and western blot , and Robo4 mRNA level was measured by real-time PCR. Results Robo4 protein and Robo4 mRNA in high dose LPS group were 0.49 ± 0.08 and 0.23 ± 0.08 respectively , which were significantly decreased than those (1.35 ± 0.15 and 0.97 ± 0.17) in control group(P < 0.05). However, there were no significant difference between the low dose (1.23 ± 0.13 and 0.94 ± 0.14) group and the control group (P > 0.05). Conclusion High dose LPS (100 μg/mL) could down-regulate expression of Robo4 in HUVECs.

Objective To evaluate the ventricular systolic and diastolic synchronicity in elderly patients with the complete right bundle branch block (CRBBB) by Doppler tissue velocity imaging (TVI) technology. Methods The 35 elderly CRBBB patients were selected as CRBBB group, while 31 healthy people were chosen as control group to evaluate myocardial function. The time to peak velocity at systolic and early diastolic phase (Ts and Te) in the base and interlude 14 segments of left and right ventricles was measured. The average time of Ts in right ventricular 2 segments(Ts-2-RV), Ts and Te in left ventricular 12 segments (Ts-12-LV and Te-12-LV), and the standard deviation in left ventricular 12 segments (Ts-12 SD and Te-12-SD), the maximum Ts and Te difference (Ts-diff and To-dill) were calculated. The left ventricular end-systolic and end-diastolic diameter (LVEDs and LVEDd) and volume (LVEVs and LVEVd) were measured. Results (1)The time to peak velocity at systolic phase in 14 segments of left and right ventricles was longer in CRBBB group than that in control group(P<0.05 or P<0.01). Compared with the control group, the Ts-12-LV,Ts-12-SD and Ts-diff showed significant prolongation (P<0.01). The Ts-2-RV was longer than Ts-12-LV in CRBBB group[(226.3±37.4 ) ms vs. (195.5±69.5) ms, P < 0.05]. (2) There was no significant difference in the time to peak velocity at early diastolic phase in 14 segments between CRBBB group and control group(P>0.05), but the Te-12-SD and Te-diff was longer in CRBBB group than that in control group (P< 0.01). (3) The LVEDs and LVEDd were obviously increased in CRBBB group compared with controls (P<0.05 and P<0.01), but no significant difference was showed in LVEVs and LVEVd(P>0.05). Conclusions Compared with normal people, the Ts at systolic phase is longer, especially in the right ventricle, the LVEDs and LVEDd are significantly increased, and the ventricular systolic and diastolic synchronicity is not well in CRBBB patients.

[Objective]To evaluate the application of sonographic measurements of the thickness of transverse carpal ligaments in CTS(carpal tunnel syndrome) patients.[Methods]Fifty-two wrists from 40 patients with CTS confirmed by surgery and 32 wrists from 20 healthy individuals(control group) underwent sonographic examination of the thickness of transverse carpal ligaments at the level of pisiform bone and the hook of the hamate bone in transverse plane by the same operator.[Results]The thickness of transverse carpal ligaments in CTS patients was(0.41?0.10) cm at hamate hook level and(0.35?0.11) cm at pisiform level versus(0.28?0.11) cm and(0.20?0.08) cm in the control group.There was significant difference in the mean value of thickness of transverse carpal ligaments hetween the two groups at the two levels.[Conclusion]Ultrasonography examination of the thickness of transverse carpal ligaments is a new and valuable technique in the diagnosis of carpal tunnel syndrome and is much helpful to choose operation methods.

Objective:To establish a system for quantitatively measuring alveolar bone density and to test its precision and validity. Methods: With the aid of computer technique, the system measured the average gray level intensity of regions of interest (ROIs) on standardized exposed direct digital periapical radiograph. To correct the variation resulting from exposure condition, an aluminum stepwedge was exposed simultaneously. By referring the image of aluminum stepwedge on each radiograph the equivalent aluminum thickness(EAT)and corrected gray level intensity(CGL)of the ROIs could be calculated as indicators of alveolar bone density. Nineteen specimens containing different amounts of hydroxy-phosphorite were radiographed. The precision of the system was tested by repeatedly measuring 2 of the specimens. The set of specimens was exposed under two different exposure time in order to test the system's validity to correct the gray level difference caused by different exposure time. Results: After repeated measurement of specimens, we found the Relative Standard Deviation(RSD) of EAT and CGL was between 0.83%-2.15%; At the level of 95%, if the difference of EAT between the two ROIs was larger than 0.05 mm or the difference of CGL was larger than 3, the content of hydroxy-phosphorite in them was different; The two sets of data during different exposure time were processed with Wilcoxon Signed Ranks Test, and the result showed effective correction of the variation caused by different exposure time. Conclusion:The precision and validity of the quantitative alveolar bone density measuring system are acceptable. The system can be used to compare alveolar bone density longitudinally and cross-sectionally.

Bidirectional signal transduction is a newly elucidated mechanism in intercellular communication. The bidirectional signal transduction mediated by the Eph-ephrin is an important representative in this field. The Eph family receptor tyrosine kinases and their membrane-bound ligands, the ephrins, play pivotal roles in the development of nervous system, angiogenesis, etc. The signal transduction into cells by Eph receptors is the forward signal, whereas the signal transduction by ephrins is the reverse signal. Based on their molecular structures, the ephrins can be divided into two subclasses, i.e. ephrinA and ephrinB. The ephrinBs are transmembrane proteins, which can activate FAK, JNK and Wnt signal transduction pathways through phosphotyrosine-dependent signaling and PDZ-binding motif-dependent signaling. The ephrinAs are glycosylphosphotidylinositol (GPI) anchored proteins, which can also mediate reverse signal transduction.

Ischemic stroke causes varying degrees of cognitive impairment, and its incidence is very high. Early diagnosis of cognitive impairment may provide the best intervention treatment opportunity, and slow disease progression. Therefore, it has attracted more and more attention. This article reviews the advances in etiology, pathogenesis, risk factors, diagnosis, prevention and treatment of cognitive impairment after ischemic stroke.

CENP-Fisacell-cycledependentkinetochoreprotein,whoseexpressionandlocalization patterns are regulated in a cell cycle-dependent manner.CENP-F plays an important role in the development and progress of malignant tumor.The expression of CENP-F is up-regulated in many tumors.It serves as an important marker in cell proliferation.Studies have demonstrated that CENP-F is involved in tumor genesis, progress,invasiveness,relapse and prognosis.CENP-F might be a valuable prognostic marker and potential therapy target in cancer treatment.

Objective To explore the relationship of course experience,course engagement and learning outcomes in medical nursing.Methods Adapted Course Experience Questionnaire and Selfestablished Course Engagement Questionnaire were used to collect data of 142 nursing undergraduates of which 126 were valid.The data were analyzed by regression analysis.Results Course experience had significant impact on course engagement,t=4.621,2.477,4.538,P＜0.01 or 0.05.Course engagement had significant impact on learning outcomes,t=2.439,P＜ 0.05.Course experience had significant impact on learning outcomes,P＜ 0.05.Conclusions It is proposed that the clinical courses should provide students with good experience,increase the deep learning engagement,and reform evaluation methods to improve the students' academic achievement.