Objective: To study the morphologic differences and nutritional components of the natural and broken Masson pine pollen. Methods: Pure pollen was collected from Masson pine (Pinus Massoniana Lamb) and the dry pollen was broken by high speed low temperature air flow technique. Their morphologic changes were observed under scan electronic microscopy. The nutritional components of pollen were analyzed.Results: The pollen particle consists of two air bags and one main part, like a mushroom cap. The main morphologic change of broken pollen was that the air bags were separated from pollen particles, and the main part of pollen particles became enriched. The physical characteristics of pollen were changed in container and its fluidity was lost. The broken pollen had very strong adhesiveness. So it could not fly like natural pollen. In broken pollen, the rough fiber, cellulose and semi cellulose contents decreased significantly. Total energy, total lipid, and starch contents increased remarkably. The water soluble and fat soluble components also increased greatly. The total amino acids content of broken pollen was higher than natural pollen except Cys, Arg and Met, and free amino acids were also higher except Cys, Gly and Met. In broken pollen, the amount of lipids extracted by ether increased sharply, nearly 7 times more than natural pollen.Conclusion: It is considered that some nutritional components of natural pollen can be released after broken. Because most of the lipids in pollen are easily oxidized, broken pollen can not be stored for long time.

The implantation of the foldable multifocal intraocular lens,a new pseudophakia intraocular substitute,needs only a small incision and the post-operative complications are rare.The incoming light can be refracted into several fo-cuses.Improved visual acuities both for far and near distance are obtained,which downregulated the glasses-wearing rate.This article summarizes the theories and designs,implantation,clinical application,theropeutic outcomes,and complications of this type of multifocal intraocular lens.

Dental Implantation ; Dental Scaling ; Diabetes Mellitus, Type 2 ; complications ; Guided Tissue Regeneration, Periodontal ; Humans ; Male ; Middle Aged ; Periodontitis ; diagnostic imaging ; etiology ; therapy ; Radiography, Panoramic

Acupuncture Points ; Acupuncture Therapy ; Adolescent ; Adult ; Aged ; Chest Pain ; therapy ; Exercise Therapy ; Female ; Humans ; Male ; Middle Aged ; Young Adult

Female ; Humans ; Hyperkeratosis, Epidermolytic ; pathology ; Infant, Newborn

The volatile oil is the main component in the leaves of Perilla frutescens. According to the main types of monoterpenoids or aromatic compounds, it can be divided into different chemotypes and the main chemotypes of Chinese producing Perilla are PA type (mainly containing Perilla aldehyde and limonene), PK type (mainly containing perillaketone) and PP type (subdivided as PP-a type, with apiole as its main component; PP-m type, with myristicin as its main component; PP-e type, with elemicin as main component; PP-as type, with asarone as main component). Based on the biosynthetic pathways analysis, we also found that the formation of the particular chemotype is usually controlled by a single gene or a few genes, and different types have different pharmacological effects. In this paper, the classification under the species P. frutescens, main chemotypes of the volatile oil, and their biogenesis and regulation, pharmacological effect and influence factors are summarized and reviewed.
Animals ; Humans ; Oils, Volatile ; analysis ; pharmacology ; toxicity ; Perilla frutescens ; chemistry ; classification ; metabolism ; Plant Leaves ; chemistry

Objective To study the effects of calcitonin on bone mineral density(BMD), bone strength and bone fragile fracture in osteoporosis. Methods This is a one year, single centered, prospective and randomized, opening study: 135 patients with osteoporosis were divided into calcitonin+calcium group and calcium group. Patients of calcitonin+calcium group received salmon calcitonin(Miacalcic was injected intramuscularly, 50 IU/day in first week, 50 IU/two days in second week and 50 IU/twice/week later) and calcium(calcium 600 mg/day), patients in another group received calcium(calcium 600 mg/day). The determination markers included serum calcium, serum phosphate, serum alkline phosphatase, osteocalcin and urine hydroxyproline before and after treatment, at same time all patients were examined by DEXA(lumbar, hip) and QUS(radius, tibia). Results After 1 year treatment, 53 patients of calcitonin+calcium group and 59 patients of calcium group were followed up. In calcitonin+calcium group, BMD of lumbar spine increased about 1%(P

Objective To explore the influence of carvedilol combined with diuretics on BNP and cardiac function of patients with mild to moderate cardiac insufficiency.Methods87 cases of patients with mild to moderate cardiac insufficiency treated in Zhejiang Greentown Cardiovascular Hospital from June 2014 to June 2016 were divided into the groups randomly.The control group of 44 cases were given carvedilol treatment, and the study group were combined with spironolactone treatment on the basis of the control group, with the total course for a month.Clinical effects were contrasted after a month of treatment in the two groups, in order to explore the influence of carvedilol combined with diuretics on BNP and cardiac function of patients with mild to moderate cardiac insufficiency.ResultsBefore treatment, there was no significant difference in serum BNP level between the control group and the study group.After treatment, serum BNP level significantly decreased when compared with those before treatment in the two groups (P< 0.05), which of the study group was significantly lower than that in the control group(P< 0.05).Before treatment, there were no significant differences in the levels of cardiac function indexes, and after treatment, heart rate, EF (%), FS (%), LVEDD (mm) the four indicators significantly improved when compared with those before treatment, and heart rate, LVEDD (mm) levels were significantly lower than those in the control group(P<0.05), and EF (%), FS (%) levels were significantly higher thatn those in the control group(P<0.05).After treatment, the significant effective rate (73.91%) and total effective rate (95.35%) in the study group were significantly higher than that of (56.82%) and(86.36%) in the control group (P<0.05).After treatment, body function, life satisfaction, mental health, social adjustment the four parts of quality of life index scores were significantly higher than those in the control group(P<0.05).ConclusionIt has obvious improvement effect of carvedilol combined with diuretics on BNP and cardiac function of patients with mild to moderate cardiac insufficiency.It is worthy of clinical application.

ObjectiveTo investigate the distribution of human immunodeficiency virus-1 (HIV-1)subtypes and the relationship with transmission routes in Shenzhen city. MethodsThe epidemiological survey was conducted in 429 subjects with HIV-1 infection.Vein blood was collected and prepared with anticoagulation.Thereafter,the viral nucleic acid was extracted and subsequently amplified for HIV-1 env,gag genes by reverse transcription nested polymerase chain reaction. The viral sequences were analyzed using Mega software and compared with those in HIV database. The HIV-1 subtypes were confirmed and evolutionary tree was established. The gene discrete rate of different subtypes was calculated.ResultsFour hundred qualified samples were identified as CRF01_AE (n=207),CRF07_BC (n=115),CRF08 _BC (n=14),CRF02_AG (n=1),B (n=49) and C(n=14),respectively.CRF01_AE was the predominant subtype that was detected in the subjects with different transmission routes,such as homosexual contact,heterosexual contact,intravenous drug use and hospital-acquired infection. Among these,sexual contact was the most common route.There were nine cases infected with subtype B HIV-1 in 10 case who were blood borne. The gene discrete rate was more significant in subtype B HIV-1 compared to the other subtypes.According to the evolutionary tree analysis,the viruses under the same subtype formed the relatively independent clusters by different transmission routes. But some overlaps were observed as well. Conclusions Tbere are many HIV-1subtypes in HIV-1 infected subjects in Shenzhen, while the recombinant viruses present as the predominant form.Transmission routes are various with sexual contact as the most common route.Every subset distributes unevenly and forms its own characteristic.Overlap can be observed among the gene distribution of different HIV-1 subtypes.

0 05) Conclusion The results suggested that pregnancy immunological tolerance can be induced efficiently and the ELR can be decreased significantly by oral administration of proper dosage antigens of OVA and TMA2