Objective To study in clinic the potential antifatigue effect of red-light irradiation onto the soldiers exhausted by a high intensity of exercise with detection of myocardial enzymes, coagulation indices and other blood routines before and after the bio-light therapy. Methods 200 soldiers selected were assigned averagely and randomly into two groups, test group and control group, with 100 for each group. All the volunteers underwent a strong intensity and exhaustion exercise. Then the red-light irradiation of 30J/cm2?30 min once a day performed 2 weeks was given to the soldiers in test group, and the soldiers in control group received similar treatment except that daylight lamp was employed instead of red-light irradiation. The blood samples were then collected from all the volunteers before the exercise and 7d and 14d after the exercise for detecting and comparing the indexes of aspartate transaminase (AST), lactate dehydrogenase (LDH), creatine kinase (CK), creatine kinase MB isoforms (CK-MB), Hydroxybutyric dehydrogenase (HBDH), white blood cell (WBC), hemoglobin (HB), red blood count (RBC), platelet (PLT), prothrombin time (PT), activated partial thromboplastin time (APTT), plasma fibrinogen (FIB), thrombin time (TT) and calcium (Ca2+). Results The findings showed an obvious increase in the indices of HB, RBC and Ca2+ of the soldiers in the test group, the indices were significantly higher than that of the soldiers in the control group. However, no significant difference was found between the two groups in such indices as AST, ADH, CK, CK-MB, HBDH, WBC, PLT, PT, APTT, FIB and TT. Conclusion As one of the harmless biotherapies, red light irradiation is conveniently and easily applied for restoring the exhausted soldiers to their normal physical ability. Without side-effects, the red light irradiation may improve the antifatigue and endurance capacity after an individual underwent a strong intensity and exhaustion exercise. It′s valuable and acceptable for enhancement of soldiers′ combat capabilities.

Objective The study is aimed to investigate the effects of radiation with red light on ATP contents in animals' liver,skeletal muscle and blood,and further to surmise the effects of the radiation on surmounting the exercise-induced fatigue.Methods 18 French white rabbits,weighed 2.0?0.5 kg,were randomly assigned to 3 groups with 6 animals for each:the animals in group A acted as control with no treatment by red light radiation;in groups B and C the animals received radiation with red light once a day and 3 hours for each time,the treatment for group B continued for one week and for group C continued for 2 weeks.Once the treatment of red light radiation ended,the venous blood were collected from the ear edge and the animals were executed,the liver tissue and skeletal muscle samples were collected,and then the contents of nicotinamide adenine dinucleotide(NAD),adenosine monophosphate(AMP),adenosine diphosphate(ADP)and adenosine triphosphate(ATP)in the samples were detected.The same collection and detection of samples were also done for the control animals.Results Compared to control group,the levels of NAD,AMP,ADP and ATP in blood samples,liver tissues and skeletal muscles in both groups B and C were significantly higher after red light radiation for certain period.Conclusion Red light radiation is one of the widely-used biotherapies with such advantages as harmless,acceptable,effectiveness on relieving pains,tension and nervousness,for its capability of inducing beneficial bio-reactions in the subcutaneous,muscular and skeletal muscles with possible improvement of exercise-induced fatigue and endurance.Since ATP serves as a key factor in supplying energy for the body and supporting basic cellular respiration,and red light radiation may increase the ATP contents in blood,tissue and muscular,so the radiation may improve the ability of anti-fatigue.

The CNKI database was searched using the keywords premature ovarian failure and acupuncture. Ninety-eight related articles published from 2006 to 2015 were retrieved and 17 with randomized controlled trials were sifted out. The recent 10 years' research situation of the mechanism of acupuncture treatment for premature ovarian failure was systematically summarized through literature review and collation. A retrospective summary was made from five aspects: improvement in ovarian endocrine function, acceleration of ovulation, effect on immunologic system, improvement in ovarian reserve function and regulation of ovarian microenvironment. It is put forward on this basis that future studies on the mechanism of acupuncture treatment for premature ovarian failure can be further into higher gene and protein levels; and no matter what kind of acupuncture manipulation is used, arrival of qi is necessary; only arrival of qi can produce an effect.

Objective To evaluate effects of human papilloma virus(HPV)16E7 on expressions of six tumor suppressor genes(including MT1G, NMES1, RRAD, SFRP1, SPARC and TFPI2)in a cell line SiHa, as well as on its proliferation and apoptosis. Methods SiHa cells were divided into two groups to be transfected with a small interfering RNA targeting HPV16E7(E7SiRNA, experimental group)and an empty vehicle(negative control group) respectively, with SiHa cells receiving no treatment serving as the blank control group. After 48 hours, qPCR was performed to measure the mRNA expressions of E7 and six tumor suppressor genes, CCK?8 assay to evaluate cellular proliferative activity, and flow cytometry to assess apoptosis of SiHa cells. Results At 48 hours after the transfection, the experimental group showed significantly decreased E7 mRNA expression(0.25 ± 0.036, P<0.05), but increased mRNA expressions of the six genes(MT1G 1.403 ± 0.190, NMES1 1.720 ± 0.060, RRAD 1.390 ± 0.160, SFRP1 1.493 ± 0.120, SPARC 2.157 ± 0.144, TFPI2 2.060 ± 0.122, all P < 0.05). The proliferative activity of SiHa cells was significantly decreased(0.554 ± 0.130 vs. 1.028 ± 0.236 and 1.220 ± 0.126, both P<0.05), but the apoptosis rate was significantly increased(9.222%vs. 0.246%and 0.123%, both P<0.05)in the experimental group compared with the negative control group and blank control group. No significant differences were observed between the negative control group and blank control group in proliferative activity or apoptosis rate of SiHa cells(both P>0.05). Conclusion E7 may participate in HPV16?induced cellular malignant transformation by suppressing the mRNA expressions of 6 tumor suppressor genes, including MT1G, NMES1, RRAD, SFRP1, SPAR and TFPI2.

ced glutathione in MHD patients appears to be associated with an improvement of oxidative stress.

Objective To investigate the effects of parathyroid hormone(PTH)on microinflammatory and nutritional status in maintenance hemodialysis(MHD)patients.Methods Ninety-eight MHD patients were selected,who hod undergone hemodialysis for at least three months before the study and were in a stable clinical status without signs of infection or disease activity.The serum level of intact PTH was measured by electrochemiluminescence immunoassay(ECLIA),while the serum levels of interleukin(IL)-1β,IL-6,IL-8 and tumor necrosis factor-α(TNF-α)were detected by enzyme-linked immunosorbent assay(ELISA).The levels of C-reactive protein(CRP),albumin(Alb),pre-albumin(PA),hemoglobin(Hb)and lipids were measured.Body measurement and modified quantitative subjective global assessment(MQSGA)was done simultaneously.Correlation analysis between serum PTH level and the parameters for inflammation and nutrition Was performed.Results The serum levels of intact PTH in MHD patients[(353.46±102.41)ng/L]were significantly higher than those in the control people[(57.45±5.76)ng/L,P<0.01],and the serum levels of IL-1β,IL-6,IL-8,TNF-α and CRP were significantly higher in MHD patients than those in the control people(P<0.01 or <0.05).Relative body weight(RBW),triceps skin fold thickness(TSF),mid-arm circumference(MAC)and mid-arm muscle circumference(MAMC)in MHD patients decreased significantly(P<0.05 or <0.01),while the score of MQSGA increased markedly(P<0.01).The levels of intact PTH showed significantly positive correlations with the levels of CRP,IL-1β, IL-6,TNF-α, lipoprotein(a) [Lp(a)] , serum phosphorus and ages of MHD(P<0.05 or <0.01 ).The levels of intact PTH showed significantly negative correlations with RBW, MAC, MAMC, Alb, Hb and total cholesterol(TC) in MHD patients (P<0.01 or <0.05) . And there was also significantly positive correlation between PTH and MQSGA in MHD patients (P<0.05). Conclusion PTH is probably involved in the presence and the progression of malnutrition-inflammation-atherosclerosis syndrome in MHD patients.

Objective To analyze the effect of acromio-humeral interval on the occurrence of subacromial impingement syndrome (SIS) after the clinical application of clavicular hook plate in acromioclavicular joint dislocation (Tossy Ⅲ). Methods From July 2005 to October 2008, 63 cases of acromioclavicular joint dislocation (Tossy Ⅲ) were treated with clavicular hook plate. There were 48 males and 15 females with an average age of 33.6 years (range, 21-53 years). The relationship of the acromio-humeral interval (AHI)were analysed between the two groups by X-ray measurement. The AHI and plate-humeral interval (PHI)were measured on X-ray film to detect difference between the two groups. Results After the operation, all t he 63 cases were divided into two groups according the occurrence of SIS. There were 14 cases in the group of SIS and 49 cases in the group without SIS. The mean follow-up was 12.2 (average, 6-15) months.The average AHI of was (8.5±2.1) mm and (9.7±2.6) mm in the group of SIS and the group without SIS respectively. The difference between the two groups was statistically significant. The average PHI of the group of SIS and the group without SIS was (6.1±2.8) mm and (6.8±2.4) mm respectively. There was no difference between the two groups. After removal of the clavicular hook plate, the clinical sign disappeared in the group of SIS. According the Karlsson scoring system, the excellent and good rate of the shoulder function was 100%. Conclusion The occurrence of SIS after the clinical application of clavicular hook plate has related to the width of the subacromial interval. The syndrome could be treated by the removal of clavicular hook plate.

Objective To explore the effect of postural reduction and balloon dilation on the correction of vertebral height following balloon percutaneous kyphoplasty (PKP) for osteoporotic vertebral coinpressive fracture (OVCF).Methods From June 2012 to December 2013,47 OVCF patients involving 57 fractured thoracolumbar vertebrae with intact posterior wall underwent PKP.In all patients postural reduction was performed under general anesthesia.The percentages of vertebral height loss before and after balloon dilation were compared.The curative effects were evaluated by comparing preoperative and postoperative visual analogue scale(VAS) scores and cobb angles.Results Compared with preoperation,the vertebral heights of anterior and middle columns were significantly improved after postural reduction,balloon dilation and PKP (P ＜ 0.05),but there was no significant improvement before and after balloon dilation (P ＞0.05).The postoperative average cobb angle (22.8°± 8.1°) was significantly decreased compared with the preoperative one (22.8° ± 8.1°) (P ＜ 0.05).The VAS scores at preoperation,one day postoperation and the final follow-up were respectively 8.5 ± 0.4,3.4 ± 0.2 and 3.1 ± 0.3.The postoperative ones were significantly lower than the preoperative one (F =7.518,P =0.006).Conclusions In PKP,postural reduction under general anesthesia plays an important role,but balloon dilation may exert an insignificant effect on restoration of vertebral height.

ObjectiveTo investigate the social support and its related factors of elderly patients with chronic obstructive pulmonary disease (COPD).Methods60 outpatients with COPD over the age of 60 years were recruited in the study. Social support rating scale (SSRS) and demographic information were applied for investigation.ResultsScores of objective, subjective support, and availability of support were lower than the norm (P<0.05). Social support was significantly different among elderly patients with age, education levels and matital status (P<0.05). Patients aged over 70 years, low education level, divorced or widowed were vulnerable groups with low social support.ConclusionEfforts should be made to strengthen the social support system for elderly patients with COPD, especially for those aged over 70 years, low education, divorced or widowed.

ObjectiveTo investigate the effects of swallowing training combined with real-time electrical stimulation on dysphagia after stroke. Methods17 patients, older than 80 years old, with dysphagia after stroke, were treated with Vocastim-Master Physiomed Elektromedizin. They were assessed with the Watian drinking water test, swallowing disorder evaluation, swallowing ability evaluation, and α. ResultsAll the assessment significantly improved in the 17 patients (P<0.001). ConclusionSwallowing training combined with real-time electrical stimulation can significantly improve the swallowing capacity of the patients older than 80 after stroke.