In the past few years, many techniques were used to study brain perfusion imaging;each of them has its own advantages and disadvantages. The principle, specification, clinical application, contradistinction and the current research situation of these techniques were reviewed in this article.

Objective To study the diagnostic value of transperineum color Doppler ultrasonography (TPCDU) and transabdominal color Doppler ultrasonography (TACDU) in pelvic varicosis (PV). Methods Thirty-eight patients with PV were detected by using TACDU and TPCDU separately, and the diagnostic value between the two ways was compared. Operational results was used as standard, and the degree of lesion in patients with PV was evaluated based on the results of surgery. Results The rate of diagnostic accuracy was high in both methods (TACDU 92% and TACDU 79%, separately). However, the rate of diagnostic accuracy and the site detectable were significantly higher by TPCDU than by TACDU (P

Objective Taking the total amino-acid, total flavones, chlorogenic acid, and volatile oil in Chrysanthemum morifolium as index to investigate the internal quality which relates to collection periods and processing methvods in order to compare the quality of species from oringinal habitats. Methods The contents of colorogenic acid in C. morifolium was analyzed by HPLC; the content of total flavones and amino-acid in C. morifolium were measured by spectrophotometery; and the volatile oil obtained by steam distilation extraction was weighted. Results The every indexes of viviparious chrysanthemum except chlorogenic acid was the best among various flowering periods so the viviparious chrysanthemum can be used as the first-class tea. The common tea produced by half-booming and full-booming flowers with higher yield and appropriate index. The machine processing is fast and suitable for the production to a large-scale. Conclusion The quality of C. morifolium planted in Ruicheng, Shanxi Province is equal to that in Tongxiang, Zhejiang Province, which depends on the collecting periods and processing methods, so does the volatile oil rather than the evironment of the habitat.

Objective To investigate the effects of benazepril on urinary excretion of endothelin 1(Et 1) and the level of plasmic Et 1 (PEt) in patients with diabetes mellitus (DM) and diabetic nephropathy(DN).Methods PEt and 24 hours urinary excretion of Et 1 (UEtV) in 54 patients with DM and 39 patients with DN before and after taking benazepril (7.5～12.5mg/d) for two months were measured using radioimmunoassay method.Results PEt and UEtV in patients with DM were significantly higher than in normal control group ( P

Objective:To evaluate the clinical sensitivity and specificity of sentinel lymph node biopsy (SLNB) for oral cancer.Methods:By searching Medline,CENTRAL,EMBSE,CBM,CNKI,WANFANG,etc,an studies about SLNB for the patients with oral cancer array for lymph node metastasis were retrieved.The quality of included studies was evaluated by QUADAS items.The data were analyzed by statistic software Meta-DiSc 1.4.Results:25 studies including 934 subjects were analyzed in the current systematic review.The pooled values of sensitivity,specificity were 91％ (95％ CI 88％-94％) and 100％ (95 ％ CI 99％-100％) respectively.The area under summary receiver operating characteristic (SROC) curve (AUC) of SLNB for cervical lymph node metastasis was 0.99.Conclusion:SLNB procedure has high sensitivity and specificity in the diagnosis of cervical lymph node metastasis,it can be used as an useful method for cervical lymph node metastasis screening in patients with oral cancer.

[Objective] Posteroanterior weight-bearing radiographs,made with the knee in 45 degrees of flexion while the patient was standing on the homolateral foot,were compared with the weight-bearing anteroposterior radiographs with both knees in full extention,in order to find a more sensitive method to detect knee osteoarthritis.[Methods]Seventeen osteoarthritis knees were radiographed in the weight-bearing anteroposterior view with both knees in full extention and in the weight-bearing posteroanterior view with the knee in 45 degrees of flexion while standing on the homolateral foot.The minimum joint space width of all the radiograph were measured.The data were analysed statistically with SPSS 11.5.[Results]The mean minimum joint space width of the medial compart in extended knee view and standing on the homolateral foot were 4.65 mm?1.44 mm and 2.52 mm?1.69 mm,respectively(P

The fluorescence characteristics of reaction products of sparfloxacin with halide (F, Cl, Br, I) have been studied. It was found that sparfloxacin was oxidized by nitrous acid then reacted with halide further to formstrong fluorescence substances in acid media. Their fluorescence intensitites enhanced with aggrandizing of atom radii of halides, which could emit the strong fluorescence 58～151 fold more than that of sparfloxacin itself. Thus a new sensitive method for the direct determination of sparfloxacin in human urine by derivative-synchronous fluorescence was presented. A plausible mchanism was proposed to explin this behavior.

Objective To investigate the incidence of adverse pregnancy outcomes among women with syphilis in Zhejiang province during 2013-2014,and to explore the influence of anti?syphilis treatment on pregnancy outcomes. Methods A retrospective study was carried out according to the Information Management System for preventing mother?to?child transmission(PMTCT)of syphilis in Zhejiang province during 2013-2014. The incidence of adverse pregnancy outcomes was compared between women with syphilis and general women without syphilis, between treated and untreated women with syphilis, as well as between women with syphilis receiving and not receiving standard anti?syphilis treatment. The effects of anti?syphilis treatment and standard anti?syphilis treatment on pregnancy outcomes were analyzed. Results Data were collected from 3 985 women with syphilis and their children. Of these women, 12 had a stillbirth, and 3 973 a live birth. Totally, 3 210(80.55%)women received anti?syphilis treatment, 967(30.12%, 967/3 210) women received substandard treatment, and 775(19.45%)did not receive anti?syphilis treatment. Of the 3 985 fetuses or neonates born to women with syphilis, 45(1.13%)had abnormal clinical symptoms/signs. The incidence rates of low birth weight and early neonatal death were 7.00%(279/3 985) and 1.13%(45/3 985) respectively in fetuses or neonates born to women with syphilis, both significantly higher than those in fetuses or neonates born to general women without syphilis (3.18%[26 092/819494] and 0.13%[1 029/819 494], respectively, both P < 0.01). Among 775 untreated women with syphilis, the incidence rates of premature birth, low birth weight, early neonatal death, congenital syphilis and abnormal neonatal symptoms/signs were 15.61%(121/775), 10.84%(84/775), 2.45%(19/775), 3.48%(27/775)and 2.84%(22/775)respectively, all significantly higher than those in 3 210 women receiving anti?syphilis therapy (8.26%, 6.07%, 0.81%, 0.81% and 1.03%,respectively, all P < 0.01). Besides, the 967 women receiving substandard anti?syphilis treatment showed higher incidence rates of premature birth, low birth weight, early neonatal death, congenital syphilis and abnormal neonatal symptoms/signs than the 2 243 women receiving standard treatment (10.44%vs. 7.31%, 8.17%vs. 5.17%, 1.55%vs. 0.49%, 1.65%vs. 0.45%, 1.96%vs. 0.62%, respectively, all P<0.01). Conclusions The incidence of adverse pregnancy outcomes is high among women with syphilis in Zhejiang province during 2013-2014. Untreated and inadequately treated maternal syphilis is strongly associated with adverse pregnancy outcomes.

BACKGROUND:At present, there stil remain controversies concerning the choice of tibial slope in unicompartmental knee arthroplasty, and the related biomechanical studies are rare. OBJECTIVE:To find the rational tibial slope in mobile-bearing unicompartmental knee arthroplasty through the results of finite element analysis. METHODS:A finite element model of normal knee was established and validated using the techniques of three-dimensional reconstruction and finite element pre-processing. Then the finite element models of unicompartmental knee arthroplasty with different tibial slopes were established based on the normal knee model. The finite element analyses were conducted after the boundary conditions and loads were unified. RESULTS AND CONCLUSION: The finite element models of unicompartmental knee arthroplasty with different tibial slopes were established successfuly. The results of the finite element analyses indicated that the posteromedial tibial cortical and cancelous bone stress increased gradualy as the posterior slope increasing, as wel as the load distribution and the cartilage contact pressures of lateral compartment. The anteromedial tibial cortical stress increased significantly with the anterior slope. A posterior tibial slope of 0°-7° can be recommended when implanting a mobile-bearing unicompartmental knee arthroplasty.

BACKGROUND:MicroRNAs (miRNAs) are widely involved in regulation of physiological processes, such as human development, cel proliferation, differentiation, and apoptosis, angiogenesis and lipid metabolism. MiRNAs also play an important regulating role in the pathological process of femoral head necrosis. At present, the research about the effect of icarin on miRNA expression in glucocorticoid- induced avascular necrosis is stil in the exploratory stage, and the specific targets, possible regulation mechanism and signaling pathway remain unclear.
OBJECTIVE:To explore the effect of icarin on miRNA expression of bone microvascular endothelial cels in steroids-induced human femoral head lesionsin vitro.
METHODS: Bone microvascular endothelial cels in cancelous bone of the femoral head were isolated and harvested in vitro. Icarin preconditioning preceded establishment of models of glucocorticoid-induced bone microvascular endothelial cel injury. Differential expression profiles and transcriptomes in glucocorticoid and normal groups were tested by miRNA microarrays. The most differentialy expressed miR-23b and miR-339 in microarray analysis were further confirmed by real-time quantitative PCR, Meanwhile the effects of icarin on the expression of miR-23b-5p and miR-339-5p were detected.
RESULTS AND CONCLUSION:According to the microarray analysis, one miRNA was up-regulated and four mi RNAs were down-regulated in the glucocorticoid group (fold > 2,P < 0.05). Results of RT-qPCR revealed that miR-23b was down-regulated and miR-339 up-regulated in the glucocorticoid group, which were in agreement with the microarray analysis (P < 0.05). Icarin pretreatment effectively prevented the imbalances of miR-23b expression induced by glucocorticoid (P < 0.01). These findings indicate that Icarin may participate in the pathological process of steroid-induced femoral head necrosis through regulating the expression of miR-23b.