1.Application of dual-energy subtraction technique in diagnosis of occupational pneumoconiosis
xie yuanlin ; huang liming ; deng xuening
China Occupational Medicine 2022;49(2):206-210
Objective To explore the application value of chest dual-energy subtraction (DES) technology in the diagnosis of
occupational pneumoconiosis. Methods A total of 86 patients with suspected pneumoconiosis and 21 dust-exposure workers
were selected as the research subjects using random sampling method. The posterior and anterior chest radiographs were taken
by digital radiography (DR) and DES technology, and the difference of chest radiographs of DR, DES and combined groups were
compared. Results The positive rate of superior chest radiographs in DR group was higher than that in DES group (72.9% vs
56.1%, P<0.05). The determination of shadow shape and size, total density and stage of pneumoconiosis on chest radiographs in
DES group and combined group were consistent with those in DR group (all Kappa values >0.75, all P<0.01). However, the
judgment of small shadow intensity, small shadow aggregation and large shadow distribution in the two groups were not superior
to those in the DR group (Kappa value was 0.67, 0.74, both P<0.01). There was no significant difference between DES group and
DR group in the determination of small shadow intensity, small shadow aggregation and large shadow distribution (P>0.05).
However, there were statistically significant difference in this index between the combined group and DES group and DR group
(all P<0.01). There was no statistically significant difference in shadow shape and size, total density and stage of pneumoconiosis
in chest radiographs among these three groups (all P>0.05). Conclusion There was no difference between DES alone and DES
combined with DR for the diagnosis of pneumoconiosis in terms of shadow shape and size, total density and stage of
pneumoconiosis when compared with the gold standard DR. The value of DES in the diagnosis of pneumoconiosis needs further
study.
2.Studies on the Activation of Germination of the Seeds of Chinese Thorowax (Bupleurum chinense)and Sandochaihu(B. falcatum)by Exogenous Hormones
Youping DENG ; Liqiang ZHAO ; Liming ZHANG
Chinese Traditional and Herbal Drugs 1994;0(07):-
Germination of the seeds of Bupleurum chinense and B. falcatum activated by exogenous hormones of dif ferent concentrations was studied. The hormones used were GA3, 6-BA and IAA. The optimum concentration of the 3 hormones that activated the seed germination of B. chinense was 50?g/ml,while that for B. falcatum was 200?g/ml, 100?g/ml and 25?g/ml respectively. Among the 3 hormones, 6-BA showed the greatest effect on the 2 species of Bupleurum L. 50?g/ml 6-BA raised the germination rate of the seeds of B. chinese from 57.5 % to 84. 5 % and 100?g/ml 6-BA raised the germination rate of the seeds of B.falcatum from 28. 5 % to 76.0%
3.The use of a self-made airway occluder in bronchoscopic lung volume reduction
Liqiang SONG ; Changgui WU ; Liming DENG
Medical Journal of Chinese People's Liberation Army 2001;0(08):-
Objective To explore the therapeutic efficacy of a self-made occluder used for bronchoscopic lung volume reduction(BLVR).Methods Two patients with severe chronic obstructive pulmonary disease(COPD)in stable phase were selected for the present study.One or two nitinol occluders were placed,guided by brochoscopy,into the target bronchi of the two patients.Examination of pulmonary functions,chest computed tomography,complications,blood gas analysis,and evaluation of life quality with St George's respiratory questionnaire(SGRQ)were carried out before and 7 months after the bronchoscopic lung volume reduction.Results No dislodging or shifting of all the inserted occluders occurred.Only slight proliferation of granulation tissue was found in mucous membrane around the occluder,and atelectasis was observed at the target sites of lung.Before and 7 months after BLVR,ventilatory capacity(VC)of case 1 rose from 3.08L to 3.57L,and that of case 2 was elevated from 2.19L to 2.58L.Forced vital capacity(FVC)of case 1 was elevated from 2.87L to 3.57L,and that of case 2 from 2.60L to 2.58L.SGRQ showed a remarkable improvement of patients' dyspnea with a decrease of scores from 62 to 26 in case 1 and from 48 to 23 in case 2.No evident changes were found on arterial oxygen blood partial pressure(PaO2)in both patients,and no severe complications such as obstructive pneumonia or pneumothorax occurred yet in either case 1 or case 2.Conclusion The present study has demonstrated that the self-made occluder is convenient for insertion with satisfactory stability and therapeutic efficacy.Application of such occluder is safe with few complications,and may serve the purpose of reducing lung volume and improving lung function.
4.Study on the Preparation of HP-?-cyclodextrin Inclusion of Volatile Oils in Anti-virus Oral Solution
Liming DENG ; Huaqing LIN ; Hong DENG ; Hang CHEN
Traditional Chinese Drug Research & Clinical Pharmacology 2000;0(05):-
Objective To optimize the HP-?-cyclodextrin(HP-?-CD)inclusion process condition for volatile oils from Anti-virus Oral Solution. Methods The process conditions were screened by determining the inclusion rate of volatile oils and the yield of inclusion compound,and by comparing the inclusion effect of supersonic method,saturated water solution method and grinding method.The optimum condition was investigated by the orthogonal test. The inclusion compound was identified by TLC,differential scanning calorimetry(DSC) and ultraviolet spectrophotometry (UV). Results The optimum preparation conditions for inclusion were established as follows: the proportion of volatile oils and HP-?-cyclodextrin was 1 ∶12,the inclusion temperature was at 45 ℃and the inclusion time was 3 hours .The inclusion rate of volatile oils was 66.71 %,and the yield of inclusion compound was 90.07 %. Conclusion The complex prepared under the optimized condition is stable and has a highest inclusion rate.
5.Study on the relationship of family functioning and subjective well-being of junior-high school students
Fang HU ; Yinghua MA ; Liming HU ; Xinlong DENG ; Jinfeng MEI
Journal of Peking University(Health Sciences) 2004;0(03):-
Objective:To describe the status of subjective well-being among junior high school students in a city of Jiangxi Province and to explore the relationship between family functioning and subjective well-being.To explore the relationship between growth environment and subjective well-being among ju-nior high school students in the perspective of family functioning and to provide basis and data for the mental health education among junior high school students.Methods:630 students in a junior high school in Jiangxi Province were sampled by stratified cluster sampling,and 103 junior high school students from 18 villages were sampled by household survey.They were investigated by subjective well-being scale for adolescents and Family Assessment Device.Results:(1)The overall subjective well-being of junior high school students was in the lower-middle level.Regarding all the dimensions of subjective well-being,they had the highest satisfaction on family and friendship,lowest academic satisfaction,low positive as well as passive emotion.(2)Girls had higher satisfaction on friendship and freedom than that of boys;children from families with more than one kids had more negative emotion than that of single child;boarding students had higher satisfaction on family,academics and freedom than that of non-boarding students;family residence and structure affected the satisfaction on family;grade and learning achievement affected some dimensions of subjective well-being among junior high school students.(3)Pearson correlation analysis and multiple linear regression analysis showed that some dimensions of family functioning were related to certain dimensions of subjective well-being among junior school students,and family functioning could predict the level of subjective well-being.Conclusion:The overall subjective well-being of junior high school students is in the lower-middle level,and further improving is needed.Family functioning has a great influence on junior school students' subjective well-being.Junior high school students who have healthy family functioning are more likely to have higher life satisfaction,and tend to experience higher subjective well-being.
6.Clinical effect of endoscopic implantation of accurate and slow-release particles for advanced esophageal carcinoma
Li WANG ; Linyan LI ; Dan HUANG ; Dongmei DENG ; Liming WEN
China Journal of Endoscopy 2016;22(9):30-34
Objective To observe the clinical effects by endoscopic implantation of accurate and slow-release particles for advanced esophageal carcinoma. Methods Under endoscopy, we dividing the whole focus into several layers by each 1.0 cm and implanting one slow-release particles (about 10 mg) in each point whose interval is 1.0 cm. One course of treatment includes three-time implantation and each treatment needs four weeks, by the way the details depend on the state of illness. In this research, all of patients complete the treatment smoothly. By comparing the patients’ situation before treatment, three-month treatment and six-month treatment, which include the remission of clinical symptom, the changing of focus area, the improvement of swallow function, untoward effect and life quality, et al. Results The results show several test indexes changed a lot. When treated for 3 months and 6 months, the levels of focus area and swallow difficulty classification were decreased significantly (P < 0.05) as compared with those before treatment; but the patients’ life quality didn’t have significant statistical difference (P > 0.05), by the way there isn’t individual difference appearing because of a little short of testing time and a few of samples. In addition, the WBC had a significant statistical difference between before treatment and three-month treatment (P < 0.05), and the ratio of complication didn’t have a significant change. Conclusions Endoscopic implantation of 5-Fu slow-release particles for the treatment of advanced esophageal carcinoma is a safety, high efficacy, low toxic and good tolerant palliative treatment.
7.Ultrastructural and functional changes of thyroid parafollicular cell in rats with acute necrotizing pancreatitis
Bo YANG ; Liming LIU ; Wenhong DENG ; Chen CHEN ; Jia YU ; Hao JIN ; Weixing WANG
Chinese Journal of Pancreatology 2012;12(4):254-256
Objective To investigate the ultrastructure and function changes of the thyroid parafollicular cell in ANP rats and to study the possible mechanism of hypocalcemia during ANP.Methods Thirty-two male Wistar rats were randomly divided into 4 groups:sham operation group ( SO group) and ANP 3,6,12 h groups.ANP model was induced by retrograde injection of 5% sodium taurocholate into the biliopancreatic duct. The serum levels of amylase,lipoidase,calcitonin and Ca2+ were measured.The pathological changes in pancreatic tissue were observed.The ultrastructure changes of thyroid parafollicular cell were observed.ResultsThe serum levels of amylase,lipoidase,calcitonin and Ca2+ were (4025 ±579)U/L,(8272 ± 794) U/L,(383.6 ± 64.5) pg/ml,(2.41 ± 0.12) mmol/L at 6h in ANP group,which were significantly higher than those in SO group [ ( 1063 ± 129 ) U/L,( 55 ± 18 ) U/L,( 143.1 ± 42.2 ) pg/ml,(2.97 ±0.12 ) mmol/L ( P <0.05 ) ].Under the electron-microscopy,the nucleus of thyroid parafollicular cells shrinked and mitochondrial proliferation and endoplasmic reticulum fusion appeared.After 6 h,all the organelle reduced and disappeared,and only small amount of secretory granules remained.Conclusions Changes in the structure and function of the thyroid parafollicular cells occur in rats of ANP,and they induce the development of hypocalcemia.
8.Evaluation of the linearity characteristic of the cone-beam CT fixed on the Varian 23EX linear accelerator
Jun ZHANG ; Liming XU ; Hui LIU ; Conghua XIE ; Yahua ZHONG ; Fuxiang ZHOU ; Gong ZHANG ; Di DENG
Chinese Journal of Radiation Oncology 2012;21(5):464-467
ObjectiveTo investigate the CT number linearity of the cone-beam CT (CBCT) images at the different spatial locations in the scanning area.MethodsThe CatphanS04 phantom at the different locations are scanned repeatcdly using the CBCT on the Varian 23EX linear accelerator.The phantom is located the isocenter point,eccentric 3 cm,eccentric 6 cm,and different points on the z-axis successively on the accelerator.The scanned mode is the standard head mode.The reconstructive thickness is 2.5 cm.The different densities inserts of CTP404 module on the different locations are measured via Eclips treatment planning system (TPS) and computed by Matlab 7.0 and the CT linear fitting are then processed.In order to understand better the linear distribution along with the value of CT in the spatial distribution the results are compared with the fan-beam CT.ResultsPhantom studies show that:CBCT has good linearity performance not only under the standard header (body) of the scanning conditions,but also on such locations including the cross-sectional,the sagittal,the coronal plane and the eccentric position ( R2 > 0.953 ).Bowtie filtration device does not change the CT finearity but changes the value of CT.ConclusionsThe linearity of X-ray CBCT on the Varian linear accelerator is favorable.CBCT will be used in the TPS dose calculation via further correction of the CT value.
9.Caveolin-1 inhibits vascular anastomotic restenosis by downregulating ERK1/2
Guozuo XIONG ; Guoshan BI ; Xianpeng DAI ; Xin SHEN ; Liming DENG ; Jie JIANG ; Hui LIU
Chinese Journal of General Surgery 2013;(2):142-145
Objective To investigate the effect of Caveolin-1 on extracellular regulated protein kinases of rabbit carotid artery anastomotic restenosis.Methods 40 New Zealand white rabbits were randomly divided into normal control group,carotid artery end to end anastomosis surgical group,empty vector transfection on the site of anastomosis group and Caveolin-1 transfected group.Left carotid artery endto-end anastomosis was performed,and the mixture of Caveolin-1 plasmid and liposome lipofectin 2000 (transfected group) or empty plasmid and lipofectin 2000 mixture (empty vector group) were transfected on anastomosis.Specimens were taken at 7 d after surgery for Western blot and RT-PCR to detect the expression of protein and mRNA.Specimens were taken for HE staining at 28 d to observe the proliferation of intima,and measured the ratio of intima/media area by Image-Pro Plus 6.0 software.Results Compared with surgical group,the ratio of intima/media area in Caveolin-1 transfected group decreased by about 50%.Compared with surgical group and empty vector group,the Caveolin-1 mRNA expression and protein activity significantly increased (t =36.59,P < 0.01) ; the ERK1/2 mRNA expression and protein activity significantly decreased on rabbit carotid artery anastomotic site in Caveolin-1 transfected group (t =32.64and 7.27,P < 0.01).Conclusions Caveolin-1 inhibits anastomotic restenosis possibly by regulating the activation of ERK.
10.Evaluation of proximal gastric function in functional dyspepsia and its subtypes
Li DENG ; Meiyun KE ; Shukun YAO ; Zhifeng WANG ; Xiaohong SUN ; Xiucai FANG ; Liming ZHU
Chinese Journal of Digestion 2014;34(6):374-378
Objective To evaluate proximal gastric function in patients with functional dyspepsia (FD) met Rome Ⅲ criteria and its subtypes.Methods Thirty FD patients were enrolled,including 15 patients with postprandial distress syndrome (PDS) and 15 patients with epigastric pain syndrome (EPS).A total of 30 healthy volunteers were recruited as control.All the subjects received barostat examination.Minimal distending pressure (MDP),pressure and volume of thresholds for first sensation and maximal tolerance for gastric sensitivity were recorded.When the pressure level was MDP+2 mmHg (1 mmHg=0.133 kPa),average volume at 30 minutes before meal,averaged volume at 60 minutes after meal and maximum accommodation volume after meal were recorded,and receptive diastolic volume was calculated.The gastric hypersensitivity and impaired accommodation were observed.Student's t test or x2 test was performed for statistical analysis.Results MDP,pressure of first-sensation,maximum sensation pressure,volume of first sensation and maximal sensation volume of FD group were (6.17 ±1.95) mmHg,(8.44±2.01) mmHg,(14.62±3.72) mmHg,(123.59±53.26) mL and (451.26±140.44) mL,respectively; which were lower than those of healthy control group ((9.27±1.99) mmHg,(12.04±2.66) mmHg,(19.74±4.18) mmHg,(168.41±73.06) mL and (556.89±124.07) mL),and the differences were statistically significant (t=-6.080,-5.900,-5.011,-2.723 and-2.995,all P<0.01).The averaged volume at 30 minutes before meal and 60 minutes after meal was (212.19±120.82) mL and (333.97±121.86) mL while those of healthy control group was (191.69±66.19) mL and (385.58±83.05) mL,and there were no significant differences between the two groups (both P>0.05).The maximum postprandial accommodation volume and receptive diastolic volume of FD group were (405.10±111.29) mL and (190.16±97.22) mL,which were lower than those of healthy control group ((461.10±87.60) mL and (262.83±78.39) mL),and the differences were statistically significant (t=-2.599 and-3.187,both P<0.05).The maximum postprandial accommodation volume of FD patients was at 15 to 20 minutes after meal and in healthy control it was at five to ten minutes after meal.Among the 30 FD patients,12 patients (40 %) had gastric hypersensitivity,including eight PDS patients and four EPS patients,and there was no significant difference in the ratio between two groups (P>0.05) ;nine patients (30 %) had impaired accommodation,including five PDS patients and four EPS patients,and there was no significant difference in the ratio between two groups (P> 0.05).Conclusions Gastric hypersensitivity and impaired accommodation often appear in FD patients,and there is no significant difference between PDS and EPS subtypes.FD patients with different pathophysiologic mechanisms cannot be efficiently distinguished according to Rome Ⅲ criteria.