Objective:To explore the effect of heredity on functional states of Thl/Th2 in cord blood mononuclear cells(CBMC)of neonates,whether their parents have anaphylactic disease. Methods:Cord blood was taken from 40 normal full term neonatals,who were divided into 20 ones with their parents having anaphylactic disease(AD group),and 20 not(control group).Collect blood plasma and cord blood mononuclear cells(CBMC)of neonates were separated,Blood plasma was collected and after stimulation with PHA,cell proliferation was detected by MTT method. IL-2,IL-4,IFN-r,and GM-CSF in supernatant were assayed by ELASA after CBMCs were cultured in vitro for 48h. Results:In AD group,as compared with control group:(1)The expression of IL-2,IL-4 and GM-CSF in blood plasma were higher than those of control group(P

The trial of two-way referral enrolled 26 patients with hypertension, including 20 cases of refractory hypertension, 3 new cases and 3 cases of secondary hypertension, the average length of follow-up was 22 d (10-35 d). The family physicians in Huaihaizhong Road Community Health Service Center communicated with specialists in the medical center (Ruijin hospital), appointments were made and medical information and other materials were sent in advance. When investigations and adjustment of medication were made, and the blood pressure was under control, patients were referred back to the community. This two-way referral system promoted the information exchanges between two side, doctors got prior knowledge about patients' condition and would spend more time with the patients; and patients' feeling of satisfaction and compliance of the treatment were increased. From our experiences, we suggest that the therapeutic regime made by the medical center should be feasible to the community, and more convenience should be given to the referred patients, so that the effectiveness and efficiency of the two-way referral can be further improved.

Colorectal cancer stem cells are a small part of cell population with features of stem cell in the colorectal cancer tissues. Tumor stem cells have the self-renewal capability and have been considered as the root of tumor recurrence. They play an important role in the development, progression, relapse, metastasis, and resistance to chemoradiotherapy of tumors. The long-term clinical practice has proved that traditional Chinese medicine has particular advantage on the aspect of resisting tumor recurrence and metastasis. In recent years, with the deep understanding of the theory of colorectal cancer stem cells, the clinical and basic researches of traditional Chinese medicine compound, single herb and active ingredient become hot spots.

OBJECTIVE To establish an optimal management procedure in order to minimize the nosocomial infection in a laminar airflow(LAF) operating theater.METHODS A comprehensive control strategy was taken to make the work-flow sequentially and effectively in the LAF operating theater.This strategy included reasonable architectural layout required for the strict standard of an LAF operating theater,daily environmental management and idealistic traffic patterns that limited the movement of personnel and supplies.RESULTS This strategy greatly improved the nosocomial infection management and effectively reduced the rate of nosocomial infection.CONCLUSIONS The good design with standard of the LAF operating theater should be firstly emphasized.A series of feasible and manageable measures must be carried out to optimize the nosocomial infection control in the LAF operating theater.

Objective To confirm the most effectively screening conditions by comparing the ARR values of different posi‐tions after saline load test .Methods After exclusion the other secondary hypertension ,the completion of saline dressing test ,84 ca‐ses diagnosed hypertensive patients under saline load test results were divided into primary aldosteronism group (pH ,52 cases) and primary hypertension group (32 cases) .Analyzed plasma aldosterone (PAC) ,renin (PRA) values of upright and decumbent body positions ,their ARR values were calculated and the urine aldosterone was tested .Results Those whose upright position ARR rati‐os higher than 240(pg/mL)/(ng · mL -1 · h-1 ) were accounted for 80 .77% (42/52) and 43 .75% (14/32) ,respectively in the both PA group and PH group;Those whose upright position ARR ratios higher than 240(pg/mL)/(ng · mL -1 · h-1 ) and decumbent position PAC higher than 150 pg/mL were accounted for 65 .39% (34/52) and 3 .13% (1/32) ,respectively in the both PA group and ph group;The specificity of this method for PA was 96 .88% .Those whose upright position ARR ratios higher than 240(pg/mL)/(ng · mL -1 · h-1 ) and decumbent position PAC higher than 150 pg/mL and urine aldosterone higher than 10 μg/24 h ,The specificity of this method for PA was 100 .00% .Conclusion It is an effective screening method for primary aldosteronism when ARR values of upright position higher than 240(pg/mL)/(ng · mL -1 · h-1 ) ,PAC of decumbent position higher than 150 pg/mL and urine aldosterone higher than 10 μg/24 h .

Objectiveto detect polymorphism of asthma related genes. Methods Patients diagnosed asthma were divided into twogroups , one group has genetic susceptibility , the other has no such character. DNA were extracted from the peripheral blood, then TG se-quence in second intron of IL-4 was amplified by PCR, the polymorphism of that segment were then detected through 8% po]yacrylamidegel. Resultfour kinds of penotype were found in such segment. The frequency of phenotype 1 and type 2 of in test group are statisticallydifferent from that in the check group. But the other two phenotype frequencies are just the same in two groups. Conclusionthe differentfrequency of phenotype I and phenotype 2 in two groups may give us some meaning in asthma genetics.

ObjectiveTo evaluate the clinical efficacy of intravenous catheter in combination with ultraviolet radiation for postherpetic neuralgia (PHN).Methods96 patients with PHN were randonly divided into three groups.Intravenous catheter plus UVB radiation was given in the test group(32 patients).Intravenous catheter or UVB radiation was only given in two control groups (32 patients/group), respectively.ResultsThe total effective rate in the test group was 96.88％ (31/32) and the average onset time was 1.55 days.However,the total effective rate in two control groups was 81.25％ (26/32) and 68.75％ (22/32) ,and the average onset time was 2.48 days and 7.41days.There was a statistical difference observed in curative rate (x2 = 5.33,42.42, P ＜ 0.05 or P ＜ 0.01), the total effective rate(x2 =4.59,8.89,P＜0.05 or P＜0.01) and the average onset time(t =22.96,11.96,all P＜0.01)between the test group and two control groups.Moreove,there was also a statistical difference observed in curative rate (x2 = 22.44, P ＜ 0.001) and the average onset time (t = 29.30, P ＜ 0.01) between two control groups.ConclusionIntravenous catheter in combination with UVB radiation was much better than intravenous catheter or UVB radiation only in the treatment of PHN.

Objective To evaluate the diagnostic value of endoscopic miniprobe ultrasonography (MPS) and endoscopic therapies under the guidance of MPS in gastrointestinal submucosal tumors. Methods Patients with submucosal tumors of lower digestive tract were given MPS under colonoscope. Endoscopic therapy or surgery had been taken according to different deriving layers of submucosal tumors. The ultimate diagnosis was confirmed by histology. Results Twenty patients with submucosal tumors were diagnosed with MPS under colonoscope, including 9 patients with stromal tumors, 5 patients with lipoma, 3 patients with car-cinoid, 2 patients with abscess,1 patient with intestines pneumatosis. Four patients with benign stromal tumors derived from muscularis mueosa and 2 patients with carcinoid were resected by endoscopy. Two patients with abscess and 1 patient with intestines pneumatosis were puncture under endoscopy. Three patients with benign stromal tumors from muscularis propria, 2 patients with malignant stromal tumors, 2 patients with lipama and 1 patient with careinoid were resected surgically. 88.24% submucosal tumors diagnosed by MPS were identified by pathology. Three cases were followed-up by endoscopy. Conclusions Different layers of gastrointestinal tract with submucosal tumors can be distinguished clearly by MPS leading to definite diagnosis of submucesal tumors. MPS can play an important role in selecting treatment procedures.

Objective To elucidate the therapeutic mechanism of 1,25-dihydroxyvitamin D3 and its analogues in psoriasis. Methods The modulating role of calcitriol, calcipotriol and tacalcitol on the expression and secretion of IL-10 in monocyte-derived dendritic cells (MDDCs) was investigated. Human monocytes were differentiated into MDDCs in vitro in the presence of GM-CSF and IL-4. Semi-quantitative RT-PCR and ELISA were used to analyze the mRNA expression and protein production of IL-10 in MDDCs. In each experi-ment, 24,25-dihydroxyvitamin D3 (a vitamin D3 metabolite with a low affinity to the vitamin D receptor) was used as a control. Results The mRNA expression and protein secretion of IL-10 in MDDCs was up-regulated by calcitriol, calcipotriol or tacalcitol with a similar potency, but not by 24,25-dihydroxyvitamin D3. The up-regulatory role of calcitriol on IL-10 protein secretion was dose dependent. Conclusion The therapeutic effect of calcitriol and its analogues in the treatment of psoriasis may be related to the induction of IL-10 production.

Objective To observe the anti-aging effect of Shenqi Wangjiang preparation on the old rats by the research on measuring serum testosterone and estradiol. Methods Measurate serum testosterone and estradiol of the old rats by the means of RIA, and observe the prevention and cure effects of Shenqi Wangjiang preparation by comparing with other groups. Result Shenqi Wangjiang preparation can enhance the serum testosterone and estradiol of the old rats. Large and middle dose have obvious effect (P