The author treated forty-eight cases of infantile cerebral palsy with scalp acupuncture and point injection. After two courses of treatment the infants made progress in different degrees. Eight cases were cured,sixteen cases were obviously improved, twenty-two cases were improved and two cases were unchanged. The total effective rate was 95.8%.

After defining chronergy for medical instruments maintenance,this paper generalizes the importance of maintenance chronergy,and puts forward some specific methods for its improvement.

Liver failure is characterized by hepatic encephalopathy, jaundice, hypo-coagulation and high mortality.Artificial liver support aims to temporarily replace hepatic function until liver function recover or bridge to liver transplantation.Individualized artificial liver support should be balanced and cover the following aspects: removing albumin bound toxins and/or water soluble toxins, eliminating inflammatory mediators and correcting blood coagulation disorders, in order to support metabolism and modulate immune function and to promote the regeneration of hepatocyte and reverse multiple organ failure that may lead to death of patients.Traditional non-biological artificial liver support can't meet individualized treatment.Recently developed hybrid blood purification model, such as albumin dialysis, fractionated plasma separation and adsorption (Prometheus system) and plasma diafiltration (PDF) have provided more and better choices for individualized artificial liver support.Based on the characteristics of pathophysiology and organ dysfunction of liver failure, the author has designed an alignment diagram of individualized artificial liver support for clinical selection.

Objective To investigate the nursing service needs of gynecological outpatient undergoing surgery as well as the level of satisfaction.Methods Eighty-eight patients undergoing surgery in the outpatient department of our hospital from March 2011 to September 2012 involved in the survey by a self-designed care service needs and satisfaction scale to investigate their needs for nursing service at pre-,intra-,and post-operation as well as their level of satisfaction with nursing service.Results The total score on nursing service needs was(3.39±0.35),falling between those on the needs and the strong needs.The needs at pre-,intra-, and post-operation were multifold.The total score on satisfaction with nursing service was(3.34±0.48),falling between those on the moderate satisfaction and great satisfaction,among which they were most unsatisfied with waiting longer than 30 mins preoperatively. Conclusions The outpatients for obstetric and gynecological consultation have needs of different aspects and the hospitals can generally meet their needs.The pertinent nursing service should be done based on the preoperative,intraoperative and postoperative needs for care service so as to improve their satisfaction.

Objective: To establish a method for the simultaneous determination of glycohyodeoxycholic acid and glycochenodeoxycholic acid in pulvis fellis suis by HPLC-ELSD.Methods: The analysis of methanol extract of pulvis fellis suis was performed on a Welch Ultimate Plus C 18 (250 mm× 4.6 mm ,5 μm)column, and the mobile phase consisted of acetonitrile and 0.1% formic acid (40∶60).The flow rate was 1.0 ml ·min-1.Alltech ELSD 2000ES was used as the detector, the drift tube temperature was 105℃ and the nitrogen flow rate was 2.5 L·min-1.Results: Glycohyodeoxycholic acid and glycochenodeoxycholic acid had good linear relationships within the range of 0.306-4.590 μg (r=0.999 5) and 0.280-4.194 μg (r=0.999 4), respectively.The average recovery was 99.23% with RSD of 2.0% and 100.37% with RSD of 1.8% (n =9), respectively.Conclusion: The method is accurate, sensitive and repeatable, which can be used for the quality control of pulvis fellis suis.

Objective:To explore the effect of heredity on functional states of Thl/Th2 in cord blood mononuclear cells(CBMC)of neonates,whether their parents have anaphylactic disease. Methods:Cord blood was taken from 40 normal full term neonatals,who were divided into 20 ones with their parents having anaphylactic disease(AD group),and 20 not(control group).Collect blood plasma and cord blood mononuclear cells(CBMC)of neonates were separated,Blood plasma was collected and after stimulation with PHA,cell proliferation was detected by MTT method. IL-2,IL-4,IFN-r,and GM-CSF in supernatant were assayed by ELASA after CBMCs were cultured in vitro for 48h. Results:In AD group,as compared with control group:(1)The expression of IL-2,IL-4 and GM-CSF in blood plasma were higher than those of control group(P

Objective To study the efficacy and safety of warfarin and aspirin in the treatment of adverse catheter function in long -term catheterization for hemodialysis.Methods 62 patients with renal failure treated with long -term central venous catheterization were selected,and all the patients were divided into the warfarin group (31 cases)and aspirin group(31 cases).Heparin in sealing was used in both two groups,and warfarin pill was given additionally in warfarin group,while aspirin pill was given additionally in aspirin group.The situation of adverse catheter function,coagulation,bleeding events,and catheter -related infections of the patients in the two groups were recorded during the time when they had 40 times of hemodialysis treatment.Results There were 3 cases of adverse catheter function in the warfarin group,the incidence rate was 9.68%,and there were 8 cases in the aspirin group,the incidence rate was 25.91%.The incidence rate in the warfarin group was lower than aspirin group(χ2 =9.081,P <0.05).The addition of urea remove index in the warfarin group was (0.13 ±0.07),which in the aspirin group was (0.04 ±0.03).The addition of urea remove index in the warfarin group was higher than aspirin group(t =0.941, P <0.05).There were no significant differences of PT,APTT and INR between the two groups(t =0.001,0.003, 0.001,all P >0.05).After treatment,there was 1 case of limb ecchymosis and 1 case of conjunctival hemorrhage in the warfarin group,and there was 1 case of conjunctival hemorrhage and 1 case of bleeding gums in the aspirin group. No catheter -related infections were found in the two groups.Conclusion Warfarin was better than aspirin in treating adverse catheter function in long -term catheterization for hemodialysis.

The authors reported the results of anatomicat study of the scapular region (ninety-four sides) and the use of the parascapular composite flap in the repair of skin defects of the hand and foot.The parascapular flap is supplied by the circumflex scapular vessels. For exposure of the vascular pedicle an incision is made 7cm below the mid-point of the scapular spine, extending from the posterior axillary fold to the mid-point of the spine. Muscles are retracted and the circumflex scapular vessels are seen winding over the axillary border of the teres minor. Then the incision is extended from both ends of the above incision toward the inferior angle of the scapula to form an elliptical incision. The flap is raised by subfascial dissection from the mid-point of the spine towards the axilla, and ended by dividing the pedicle at the base of the circumflex scapular vssels. Since October 1982, the authors have applied flaps of such kind to repairing massive skin delects in the hand and the foot in 7 patients with success.

Atypical hemolytic uremic syndrome(aHUS) has recently been shown to be a rare disease of genetic predisposition, including genes of complement factor H(CFH), membrane cofactor protein(MCP, CD46)and complement factor Ⅰ(CFI), which are complement regulatory genes. Genes mutation is about 50%,involving the three genes mutation, including nonsense mutation, missen mutation, silent mutation, splice mutation and insertion mutation. Autosomal dominant inheritance and autosomal recessive inheritance have been reported, however, for autosomal dominant inheritance, the three genes mutations are incomplete penetrance.

Comparison between the DNA fingerprinting and the serological method used for paternity test is presented. 32 cases of disputed paternity were tested using DNA fingererints with MYO minisatellite DNA probe and blood typing. The results of DNA fingerprinting test correlated with those of blood typing. 8 alleged fathers were excluded while 24 were confirmed. The relationship between the results from two methods was discussed.