Objective To investigate the effects of octreotide (OCT) on gene and protein expressions of collagen Ⅰ,collagen Ⅲ and matrix matrixmetlloproteinases 2 (MMP-2)in hepatic stellate cells(HSC) in vitro,and to clarify the mechanism of its anti-hepatic fibrosis.Methods A rat hepatic stellate cell line,rHSC-99,was treated with OCT.Cell proliferation was assessed by MTT colorimetric assay.The contents of collagen Ⅰ,Ⅲ were examined by ELISA;RT-PCR was used to detect mRNA expressions of MMP-2,collagen Ⅰ and collagen Ⅲ.Results Compared with control group,OCT significantly inhibited rHSC-99 proliferation in vitro (P

Objective To observe the therapeutic effect of Chinese medicine combined with ion-introduction of Radix Astragali Injection (RAI)for herpes simplex keratitis (HSK).Methods Ninety HSK patients (104 affected eyes)were randomized into two groups.Both of the two groups received anti-virus drugs,and the treatment group (45 patients with 53 affected eyes)received oral use of decoction of Chinese medicines (mainly composed of Semen Cassiae,Fructus Viticis,Periostracum Cidadae,Ramulus Uncariae cum Uncis,Fructus Gardeniae,Fructus Forsythiae,Flos Chrysanthemi,Rhizoma Chuanxiong,etc.),and ion-introduction of RAI.The changes of vision and clinical symptoms before and after treatment were observed,and a 2-year follow-up was carried out.Results In the treatment group,19 eyes were cured,27 effective,7 ineffective,and the total therapeutic effective rate being 86.79%;in the control group,10 eyes were cured,21 effective,20 ineffective,and the total therapeutic effective rate being 60.78%.The therapeutic effect in the treatment group was superior to that in the control group (P