Objective To compare the growth-promoting effects of platelet lysate-containing newborn calf serum(PL-NBCS) and fetal bovine serum(FBS) on cultured BHK-21,Vero,HEK293T cells,and to provide experimental basis for exploring the feasibility of culturing cells with PL-NBCS instead of FBS.Methods BHK21 cells,Vero cells and HEK293T cells were cultured with PL-NBCS and FBS,respectively.The growth-promoting effect of PL-NBCS on cells was detected by kinetic statistical analysis of morphological observation,average cell proliferation density,viability and doubling time of continuously subcultured cells.Results There was no significant difference in morphological characteristics between three kinds of cells cultured in PL-NBCS and those cultured in FBS.Under the two culture conditions,the cells grew in an "S" shape.The average proliferation density,viability and doubling time of BHK-21 cells cultured in PL-NBCS showed no significant difference with the BHK-21 cells cultured in FBS(t=0.734,0.432 and 0.029,P=0.472,0.671 and 0.977,respectively).The average proliferation density and viability of Vero cells cultured in PL-NBCS were higher than those in FBS group,and the average doubling time was significantly higher than that in FBS group(t=2.228,P=0.042).The average proliferation density,viability and doubling time of HEK293T cells cultured in PL-NBCS were significantly higher than those in FBS group(t=2.309,2.848 and 2.682,P=0.033,0.011 and 0.015,respectively).Conclusion PL-NBCS has better growth-promoting effect on cells,and can replace FBS to subculture cells.

Objective To investigate the clinical efficacy of acupuncture plus Chinese herbal medicine in treating allergic rhinitis. Methods One hundred and thirty-five patients with allergic rhinitis were randomly allocated to combination, acupuncture and Chinese herbal medicine groups, 45 cases each. The combination group received treatment with acupuncture plus Chinese herbal medicine; the acupuncture group, treatment with acupuncture alone; the Chinese herbal medicine group, treatment with Chinese herbal medicine alone. IgE, IL-4, IL-5 and TNF-αlevels and clinical symptom and sign total score were observed in the three groups before and after treatment. The clinical therapeutic effects were compared between the three groups. Results There were statistically significant pre-/post-treatment differences in IL-4, IL-5, TNF-αand IgE levels in the three groups (P<0.05). There were statistically significant post-treatment differences in IL-4, IL-5 and IgE levels between the combination group and the Chinese herbal medicine or acupuncture group (P<0.05). There was a statistically significant pre-/post-treatment difference in clinical symptom and sign total score in the three groups (P<0.05). There was a statistically significant post-treatment difference in clinical symptom and sign total score between the combination group and the Chinese herbal medicine or acupuncture group (P<0.05). The total efficacy rate was 91.1%in the combination group, 71.1%in the Chinese herbal medicine group and 66.7%in the acupuncture group. There was a statistically significant difference in the total efficacy rate between the combination group and the Chinese herbal medicine or acupuncture group (P<0.05). Conclusions Acupuncture plus Chinese herbal medicine has a better therapeutic effect on allergic rhinitis. It can effectively relieve the clinical symptoms and signs and reduce IgE, IL-4 and IL-5 levels in the patients.

Objective To study on extraction and characteristics of ?-glucosidase inhibitor from Chlorella vulgaris.Methods The extracts were prepared by drying,degreasing and silica gel columniation washing with different organic solvents,and its pure degree and components were identified.The activities of the inhibitor and its effects on ?-glucosidase under different conditions were studied.Results The results showed that ?-glucosidase inhibitor from Chlorella vilgaris may be a kind of substance of hydroxybenzene.Conclusion The inhibitor showed potent inhibitive effection on ?-glucosidase,and belong to a mechanism of noncompetitive inhibition

Objective Macrophages are important innate immune cells,and are also the main target cells of Mycobacterium tuberculosis.Macrophages express many kinds of Toll like receptors.TLR2 is a structural component of macrophages in the identification of Mycobacterium tuberculosis.Activation of TLR2 signaling pathway can activate cell signal transduction,induce specific gene expression and secretion of cytokines.Immune function decline in old people and the function of macrophages was also changed.In this article,The role of immunity mediated by TLR2 in macrophages against tuberculosis in the elderly is reviewed.

AIM: To optimize the parameters of processing for Arctium Lappa.METHODS: The contents of arctiin and arctigenin in Arctium Lappa were determined with HPLC,and the total yields of them were chosen as in-dex.The orthogonal test was adopted to acquire the optimum technology for processing Arctium Lappa.RESULTS: The study showed that the best processing parameters for Arctium Lappa consisted of 300 ℃ of the drying tempera-ture and 4-5 minutes of processing time.CONCLUSION: The processing method is reasonable,stable and reliable,and can be used in commercial production.

AIM:To establish an HPLC fingerprint spectrum of Red Peony Root ( Radix paeonia rubra). METHODS:Ten batches of samples were extracted by 50% ethanol and extract liquor was evaporated to dryness,resolved with methanol. The HPLC analysis was performed on a Diamonsil C18 column with the eluting system of gradient acetonitrile and water. MS was applied to the determination of possible chemical structure. RESULTS:Nine main characteristic peaks were selected from the fingerprint spectrum of 10 batches of samples. Five constituents of which were identified as gallic acid,peoniflorin albiflorin,benzoylpaeoniflorin and paeonol based on the MS spectra. CONCLUSION:The method is valuable for quality conthol of Red Peony Root.

Objective To verify the detection performance of glycated hemoglobin (HbA1c)by using the BIO-RAD D-10 high performance liquid chromatography method,and to evaluate whether the detection performance can satisfy the requirements of the laboratory and clinical diagnosis and treatment.Methods The method of CLSI EP1 5A2 was used to verify the accuracy and preci-sion.The linear correlation and reference range were verified respectively by linear regression analysis and the method of the WS/T 402 2012.Results The calibration value of HbA1c calibrator level 1 was 5.3% and the validation limit of measured results was 1. 63%-8.78%.The calibration value of HbA1c calibrator Level 2 was 10.0%,and the validation limit of measured results was 4.76%-13.64%.Both calibration values were included in the 95% confidence range of measured values,so the accuracy verifica-tion passed.Laboratory variation coefficient (CV)for HbA1c with concentration of 5.24% was 1.01% and for 9.84% was 0. 54%,both less than the precision that the manufacturer declared,so the precision verification passed.The equation of linear regres-sion analysis for HbA1c linearity calibrator measured value and target value wasY =1.008X +0.023,with correlation coefficient in-dex r 2 of 0.999,so the linearity was good.The measured results of 20 healthy adults specimens were in the reference interval(4.1%-6.2%)that the manufacturer provided.Conclusion Accuracy,precision,linear correlation and reference range of HbA1c meas-ured by BIO-RAD D-10 HPLC detection method were verified.It can provide the accurate and credible measurement results for clin-ical diagnosis and treatment,and can be used in the detection of clinical specimens.

Objective To study the curative effect and mechanism of moxibustion on heat-sensitive acupoints combined with Erwei Badu Powder in the treatment of elderly herpes zoster (HZ) with type of stagnant heat of liver channel. Methods Sixty-five patients with HZ were randomly divided into treatment group (35 cases) and control group (30 cases). Two groups were orally given Longdan Xiegan Decoction and aciclovir conventionally, the treatment group was given moxibustion additionally on heat-sensitive acupoints combined with Erwei Badu Powder, and the control group was only given external application of Erwei Badu Powder for 10 days. The symptom scores, levels of T lymphocyte subsets and serum interleukin-2 (IL-2) were observed before and after treatment, the curative effect was evaluated, and PHN was followed up. Results The effective rate was 85.71%(30/35) in the treatment group and 66.67%(20/30) in the control group. The curative effect of treatment group surpassed the control group (P<0.05). Incidence of PHN was lower (P<0.01). The levels of CD4, CD4/CD8, and IL-2 increased and the level of CD8 decreased significantly in two groups compared with those before treatment (P<0.05). All indexes were better in the treatment group than those in the control group (P<0.05). Conclusion The curative effect of moxibustion on heat-sensitive acupoints combined with Erwei Badu Powder in treatment of elderly HZ is better than that of Erwei Badu Powder only, particularly in reducing the occurrence of PHN, improving neuralgia and insomnia. The mechanism may be related to increasing levels of CD4, CD4/CD8 and IL-2, and improvement of body’s cell-mediated immunity.

Objective To investigate correlation study of serum hs-CRP,UA level and the correlation of dis-ease in elderly acute myocardial infarction patients , and provide a reference basis for clinical judgment for patients with illness development and prognosis prediction .Methods 40 aged patients with acute myocardial infarction pa-tients were selected as the research object ,and protionally random according to time admission the same period ran-domly selected were 40 cases of elderly patients with unstable angina pectoris ,40 cases of elderly patients with stable angina,40 cases of clinic medical normal healthy old people for controlled object .The levels of serum hs-CRP,UA and correlation was analyzed.Results The serum hsCRP,UA in the myocardial infarction group were (34.67 ±12.18)mg/L, (412.46 ±68.93)μmol/L,which in the unstable angina pectoris group were (17.21 ±8.39) mg/L,(274.19 ± 46.62)μmol/L,which in stable angina pectoris group were (5.25 ±1.40) mg/L,(297.41 ±37.43)μmol/L,which in the health group(4.96 ±1.24)mg/L,(279.64 ±34.78)μmol/L,the differences among the myocardial infarction group,unstable angina pectoris,stable angina pectoris group had statistical significance (P<0.05), the differences between myocardial infarction group and health group had statistical significance (P<0.05);Myocardial infarction patients prognosis group hs-CRP,UA group was obviously higher than that of the prognosis was good ,compared with statistical significance difference ( P <0.05 );In myocardial infarction group , unstable angina pectoris group , the hsCRP was associated with positive correlation exists between UA (P<0.05),stable angina pectoris group no correla-tion(P>0.05);Different prognosis in patients with myocardial infarction hsCRP is associated with UA has positive correlation between ( P<0.05) .Conclusion Elderly acute myocardial infarction patients serum hs-CRP,UA obviously increased ,and illness development correlation ,a high level of hs-CRP,UA patients often indicates poor prognosis .

Objective To determine the content of berberine hydrochloride in Shaoyao decoction. Methods HPLC was performed on an Agilent XDB C18 column by using acetonitrile-0.05 mol/L potassium dihydrogen phosphate-triethylamine (30∶70∶0.14) as mobile phase at a flow rate of 1.0 mL/min, the column temperature was 25 ℃ and the UV detection wavelength was 345 nm. Results Berberine hydrochloride showed a good linearity redationship in the range of 0.07～1.40 ?g. The average recovery was 98.63% (n=6, RSD=1.21%). The average content was 1.55%. Conclusion The method is simple and accurate, with good repeatability, and can be used for the quality control of Shaoyao decoction.