Objective To isolate and cultivate hepatic stem cells(HSCs) from rat fetal liver in vitro and identify their biological features.Methods Collagenase perfusion method and mechanical cutting method were used to isolate HSCs from rat fetal liver which were then cultivated by H-DMEM containing 10% fetal bovine serum.The cell surface antigen expression of HSCs was observed with immunocytochemical method under confocal laser scanning microscope.Results The isolated HSCs from rat fetal liver grew to monolayer 5 d after cultivation in vitro.They presented pykno-round cells and distinct borderline under the light microscope.After 8 d the cells grew like epithelium.These cells expressed AFP antigen,CK 18 and CK19.Conclusion The cultivated cells are proved to be HSCs and can proliferate quickly in vitro.

Objective To detect the genetic amplification and protein expression characteristics of aurora-A in ovarian cancer and to interpret the role of aurora-A gene in course of onset,progression and regression phases of ovarian cancer.Methods The amplification of aurora-A gene was detected by quantitative PCR in 6 normal ovarian tissues and 8 ovarian cancer samples,and its protein expression was examined by immunohistochemistry in 6 normal ovarian tissues and 40 ovarian cancer samples,furthermore,the relationships between over-expression of aurora-A protein in ovarian cancer tissue and its pathologic classification,tissue differentiation,clinical phase,tumor proliferation trait and prognosis were analyzed.Results Quantitive PCR showed that aurora-A mRNA was significantly higher in 8 ovarian cancer samples than that in normal ovarian tissues(P0.05).Conclusion There are abnormal amplification and protein over-expression of aurora-A in ovarian cancer tissue,aurora-A probably play an important role in the onset and progression of ovarian cancer,and the novel biological treatment concerning aurora-A gene and its protein is probably a useful route for curing tumor.

95% purity were treated for 24 h with 1,5,10 and 20 ?mol/L 5-azacytidine to induce cellular differentiation.Expression of the cardiac-specific marker-troponin Ⅰ and desmin,identified by immunohistochemistry,was used to identify cardiac muscle differentiation.Results:hMSCs expressed a high level of CD44(hMSCs 93.26%?2.48% vs 3.42%?1.09% in a control group,P

Brain Neoplasms ; pathology ; Humans ; Pituitary Neoplasms ; pathology

Humans ; Lymphoma, Large B-Cell, Diffuse ; pathology ; Pituitary Gland ; pathology

Bone Neoplasms ; pathology ; Cerebellum ; pathology ; Humans ; Neurocytoma ; pathology ; Petrous Bone ; pathology

Abdominal Pain ; etiology ; Acute Pain ; etiology ; Child ; Humans ; Lymphoma ; complications

Objective: To explore the distribution and intensity of kitchen waste treatment plant, providing scientific basis for occupational diseases prevention and treatment in that industry. Methods: 30 operating personnel in a kitchen waste disposal enterprise were selected as the research group and 16 non-operating personnel as the control group. The epidemiological survey was carried out in June 2015 and July 2016, and the results of occupational hazards and occupational health monitoring during 2015 to 2017 were analyzed. Results: The results of various occupational hazards factors during 2015 to 2017 were all in accordance with the occupational health limit, but the results of occupational health examination were abnormal. The occupational correlation could not be ruled out by the abnormality of electrical audiometry, electrocardiogram and Department of Dermatology examination. Conclusion Workers in kitchen waste treatment plant are likely to suffer low-intensity, continuous health damage. There is occupational health risk in that industry. The accumulation of basic information and the improvement of relevant standards and methods are needed for further study.

Objective:To explore the correlation between serum soluble T cell immunoglobulin-mucin molecule-3 (sTim-3), galactose lectin-9 (Galectin-9) and inflammatory factors interferon-γ (IFN-γ), interleukin-4 (IL-4) in patients with brucellosis.Methods:Ninety patients with brucellosis admitted to Yantai Yeda Hospital from January 2016 to December 2018 were selected as the observation group, of whom 49 cases in acute stage and 41 cases in chronic stage. Another 50 healthy subjects who underwent health examination during the same period were selected as the control group. Five milliliter of fasting venous blood samples were collected from patients with brucellosis and healthy subjects, and enzyme-linked immunosorbent assay (ELISA) was used to detect sTim-3 and Galectin-9 levels, and cytometric bead array (CBA) to detect IFN-γ and IL-4 levels; Pearson correlation test was used to correlate serum levels of sTim-3, Galectin-9, sTim-3/Galectin-9 and inflammatory factors IFN-γ, IL-4 in patients with brucellosis.Results:The levels of sTim-3 [(18.28 ± 5.38), (7.01 ± 1.97) ng/L], Galectin-9 [(2.79 ± 0.94), (1.67 ± 0.23) ng/L], sTim-3/Galectin-9 (6.55 ± 2.96, 4.20 ± 0.67), IFN-γ [(5.99 ± 1.94), (10.09 ± 2.03) ng/L], IL-4 [(7.34 ± 3.13), (3.71 ± 1.76) ng/L] were significantly different between the observation group and control group ( t = 14.271, 8.276, 5.527, 11.785, 7.556, P < 0.05). In the observation group, the levels of sTim-3 [(22.09 ± 3.13), (14.21 ± 3.42) ng/L], Galectin-9 [(2.98 ± 0.67), (2.41 ± 0.74) ng/L], sTim-3/Galectin-9 (7.41 ± 1.07, 5.90 ± 1.72), IFN-γ [(3.78 ± 1.65), (7.02 ± 1.73) ng/L], and IL-4 [(9.17 ± 2.04), (5.67 ± 1.54) ng/L] were statistically significant difference between patients with acute and chronic brucellosis ( t = 11.402, 3.833, 5.084, 9.075, 9.037, P < 0.05). Pearson correlation test showed that the levels of sTim-3, Galectin-9, and sTim-3/Galectin-9 in patients with brucellosis were negatively correlated with IFN-γ level ( r = - 0.568, - 0.531, - 0.519, P < 0.05), and positively correlated with IL-4 level ( r = 0.517, 0.524, 0.536, P < 0.05). Conclusion:The expressions of sTim-3 and Galectin-9 in serum of brucellosis patients are high, and they are significantly correlated with IFN-γ and IL-4 levels, suggesting that sTim-3 and Galectin-9 maybe related to the occurrence and development of brucellosis.

Objective:To establish the normal reference value of lumbar bone mineral density (BMD) under quantitative CT (QCT) in Chinese healthy adult females and to explore the regional differences.Methods:Total of 35 431 healthy women who met the inclusion criteria of Chinese health quantitative CT big data program were selected in this study. The BMD of the central plane of L 1 and L 2 vertebrae was measured by Mindways′s QCT system, and the mean value was taken. One-way analysis of variance was used to compare the BMD differences of lumbar vertebrae in women of different ages and regions. The subjects were grouped by an age interval of 10 years, and the level of BMD in different regions of the same age group were compaired. Results:The peak BMD of Chinese healthy adult women appeared in the age group of 20-29 years (Northeast China(183.01±24.58) mg/cm 3, North China (188.93±24.80) mg/cm 3, East China (187.54±27.71) mg/cm 3, South China (186.22±33.72) mg/cm 3, Central China (176.33±24.91) mg/cm 3, Southwest China(182.25±28.00) mg/cm 3), and then it decreased with age. The level of BMD in different regions decreased with the age. Before the age of 70 years, BMD in Central and Southwest China was always at a low level((176.23±24.91) to (90.38±28.12) mg/cm 3, 182.25±28.00 to (88.55±25.68) mg/cm 3), lower than those in Northeast China ((183.01±24.58) to (99.69±27.85) mg/cm 3), North China ((188.93±24.80) to (95.89±26.12) mg/cm 3), East China ((187.54±27.71) to (95.65±27.86) mg/cm 3). After 70 years of age, BMD tended to be the same in different regions ( P>0.05). The BMD values in Central China and Southwest China were similar in the age group of 40-60 years ( P>0.05). The BMD values in the health adult femles in the age group of 60 years in different regions of Chinawere all lower than those of bone mass abnormality (all P<0.05). The detection rate of osteoporosis in females over 50 years was the highest in Southwest China (25.65%) and it was the lowest in North China (17.30%). Conclusions:This study establishes reference values of BMD under QCT in healthy Chinese women, which can be used as a reference basis for identifying women with low BMD who are at risk of osteoporosis. The BMD value is the lowest in Southwest China and the highest in South China.