Objective To investigate the clinical efficacy of acupuncture plus Chinese herbal medicine in treating allergic rhinitis. Methods One hundred and thirty-five patients with allergic rhinitis were randomly allocated to combination, acupuncture and Chinese herbal medicine groups, 45 cases each. The combination group received treatment with acupuncture plus Chinese herbal medicine; the acupuncture group, treatment with acupuncture alone; the Chinese herbal medicine group, treatment with Chinese herbal medicine alone. IgE, IL-4, IL-5 and TNF-αlevels and clinical symptom and sign total score were observed in the three groups before and after treatment. The clinical therapeutic effects were compared between the three groups. Results There were statistically significant pre-/post-treatment differences in IL-4, IL-5, TNF-αand IgE levels in the three groups (P<0.05). There were statistically significant post-treatment differences in IL-4, IL-5 and IgE levels between the combination group and the Chinese herbal medicine or acupuncture group (P<0.05). There was a statistically significant pre-/post-treatment difference in clinical symptom and sign total score in the three groups (P<0.05). There was a statistically significant post-treatment difference in clinical symptom and sign total score between the combination group and the Chinese herbal medicine or acupuncture group (P<0.05). The total efficacy rate was 91.1%in the combination group, 71.1%in the Chinese herbal medicine group and 66.7%in the acupuncture group. There was a statistically significant difference in the total efficacy rate between the combination group and the Chinese herbal medicine or acupuncture group (P<0.05). Conclusions Acupuncture plus Chinese herbal medicine has a better therapeutic effect on allergic rhinitis. It can effectively relieve the clinical symptoms and signs and reduce IgE, IL-4 and IL-5 levels in the patients.

Objective:To compare and analyze the volatile components in different medicinal parts of silktree Albizzia julibrissin and their relative contents .Methods:Chromatography-mass spectrometry using headspace solid phase microextraction combined with gas method ( HS-SPME-GC-MS) was used to analyze the volatile components in the bark and flowers of silktree Albizzia julibrissin and the relative contents were calculated by a peak area normalization method .Results:In the bark and flowers of silktree Albizzia julibris-sin, totally 30 and 33 compounds were respectively identified , which respectively accounted for 73.20%and 88.29%of the total vola-tile.A total of 15 components, including alkanes, alkenes, aldehydes, furans, alcohols and acids were in the bark and flowers of silk-tree Albizzia julibrissin, among them, the content of cineole was the highest (bark:15.02%and flowers:21.9%).Conclusion:The HS-SPME technique can be used to analyze and compare the volatile components in the bark and flowers of silktree Albizzia julibrissin, which provides scientific basis for the reasonable development and application of the bark and flowers of silktree Albizzia julibrissin.

Objective To evaluate the effects of nutritional intervention on the incidence of malnutrition in patients on maintenance hemodialysis (MHD).Methods Totally 196 patients on MHD were randomly assigned to nutritional intervention group (n =98) and control group (n =98).Nutritional intervention or free diet were given in these two groups.The incidence of malnutrition in these two groups was recoded.Results The baseline age,biochemical indicators,and incidence of malnutrition were matched in these two groups (P ＞0.05).After 6 months,although the number of the patients with malnutrition [35.7％ (35/98)] was slightly increased in the intervention group,it showed no significant difference when compared with the baseline level [33.7％ (33/98),P ＞0.05].In the control group,however,the incidence of malnutrition [59.2％ (58/98)] significantly increased (P ＜0.05).The incidence of malnutrition was significantly lower in the intervention group than in the control group (P ＜ 0.05).Conclusion Nutritional intervention can lower the incidence of malnutrition in patients on MHD.

Researchers have long focused on the adaptive behavior,quality of life,dual diagnosis,personality motivation and families of persons with mental retardation,but failed to address virtue and well-being.Unlike even a decade ago,we can now well afford to turn the emphasis to the positive,internal states of those with mental retardation.Using the breakthroughs in positive psychology,these findings such as positive trait or positive experience,can proposes a new research agenda to mental retardation.Mental retardation can play an important role in positive psychology,but it needs the research and data support in future.

OBJECTIVE:A HPLC method has been developed to determine the concentration of isoniazid in plasma.METH_ODS:The Eclipse XDB-C18 column was used as fix phase and acetonitrile-0.05mol/L ammonium dihydrogen phosphate as mo_bile phase,detection wavelength was 280nm.The plasma sample was injected directly for determination after being deproteinized with 10% trichloroacetic acid and reacted with cinnamaldehyde and abstracted with ether.RESULTS:Good linear relationship was shown from 0.10 to 12.0?g/ml and the averge recovery of isoniazid was 95%～105%.CONCLUSION:The method is rapid,sensitive and is rarely interfered so it can be used in study of pharmacokinetics of isoniazid.

OBJECTIVE:To study the pharmacokinetics and relative bioavailability of compound rifampicin tablets MET_HODS:Plasma levels of rifampicin(RFP),isoniazid(INH)and pyrazinamid(PZA) at different time were determined by HPLC methods,then we drew the time-concentration curves and got the pharmacokinetic parameters and relative bioavailability of test-tablets based on the curves RESULTS:The main pharmacokinetic parameters of RFP,INH and PZA in test-tablets were:Tmax,(1 69?0 60)h,(0 94?0 57)h and(2 36?1 10)h;Cmax,(9 86?2 09)?g/ml,(5 36?1 77)?g/ml and (16 20?4 85)?g/ml;T1/2,(3 43?0 72)h,(2 98?0 75)h and(9 26?1 58)h;AUC0～t,(59 34?13 17)?g/(ml?h),(21 87?14 29)?g/(ml?h) and(212 97?71 52)?g/(ml?h) respectively The main pharmacokinetic parameters of RFP,INH and PZA in control tablets were Tmax,(1 83?0 66)h,(0 86?0 38)h and (2 08?0 97)h;Cmax,(9 98?1 63)?g/ml,(5 60?2 01)?g/ml and (17 79?4 57)?g/ml;T1/2,(3 97?1 58)h,(3 15?0 88)h and (9 36?1 85)h;AUC0～t,(62 46?14 02)?g?h/ml,(21 39?14 53)?g/(ml?h) and (227 09?70 91)?g/(ml?h) respectively The relative bioavailability of test-tablets were (98 47?15 00)%,(103 76?15 80)% and (94 38?12 07)% CONCLUSION:The results of two one-sided tests and rank sum test showed that two formulae were statistically bioequivalent

OBJECTIVE:To evaluate the bioequivalence of two preparations of gemfibrozil.METHODS:A single oral dose of gemfibrozil enteric capsule(test preparation)and capsule(reference preparation)was given to20volunteers in an open ran?domized crossover way to study the pharmacokinetics and relative bioavailability.The plasma gemfibrozil concentrations were determined by HPLC method.RESULTS:The pharmacokinetic parameters of test and reference preparations were as follows:T max ,(2.4?0.6)h and(2.3?0.7)h;C max ,(21.8?7.3)?g/ml and(23.7?5.9)?g/ml;T 1/2 ,(2.0?0.4)h and(2.0?0.5)h;AUC 0～12 ,(68.1?13.7)(?g?h)/ml and(68.9?17.4)(?g?h)/ml;AUC 0～∞ (69.7?13.9)(?g?h)/ml and(70.6?17.8)(?g?h)/ml respectively.The relative bioavailability of test preparation was(100.8?15.0)%.The result of statistical analysis on above parameters showed that there was no significant difference between two preparations.CONCLUSION:The two prepa?rations were bioequivalent.

Objective:To develop an HPLC-MS/MS method for the determination of rosuvastatin in plasma and study the relative bioavailability and bioequivalence of the capsules and tablets in Chinese healthy volunteers. Methods: A single oral dose (20 mg of the test or reference preparation) was given to 24 male healthy volunteers in a randomized crossover study. The plasma concentration of rosuvastatin was determined by HPLC-MS/MS. The pharmacokinetic parameters were calculated and the bioavailability and bioequiva-lence of the two preparations were evaluated by DAS 3. 0 software. Results:After a single dose, the pharmacokinetic parameters of ro-suvastatin capsules and tablets were as follows:Tmax was (3. 56 ± 1. 68) h and (3. 63 ± 1. 56) h, Cmax was (21. 17 ± 13. 74) ng· ml-1 and (26.33 ±23.22) ng·ml-1, t1/2 was (10.68 ±5.50) h and (9.04 ±6.00) h, AUC0-t was (219.31 ±146.09) ng·h· ml-1 and (252. 43 ± 194. 96) ng·h·ml-1 , AUC0-∞ was (225. 32 ± 146. 76) ng·h·ml-1 and (257. 24 ± 194. 61) ng·h·ml-1 , respectively. The 90% confidential interval of AUC0-t, AUC0-∞ and Cmax was 81. 1%-106% , 81. 8%-105. 4% and 77. 9%-104. 5%, respectively. The mean relative bioavailability of the test preparation(the capsules) to the reference preparation(the tablets) was (100. 7 ± 54. 1)%. Conclusion:The test and reference preparations are bioequivalent.

A label-free electrochemical immunosensor using hollow structure nanomaterials based on its ordered porous and big surface area was designed. Au nanocage, with good conductivity, catalysis, and biocompatibility, was prepared and modified on the surface of glassy carbon electrode with graphene to immobilize antibody of microcystin directly. In the absence of microcystin, biosensor can obtain high current response signal of electrochemical probe ( [ Fe( CN) 6 ] 3-/4-. When microcystin was combined with its antibody specifically, the charge density and mass transfer resistance on the surface of electrode increased, resulting in a decrease of the corresponding peak current of [ Fe ( CN ) 6 ] 3-/4-. This change was in proportion to the concentration of microcystin indirectly. Experiment conditions such as cultivation time of antigen and concentration of antibody were optimized. The results showed wide linear range of 0. 05 μg/L-1. 0 mg/L and the detection limit of 0. 017 ng/mL. This sensor has good stability and simple production procedure. This sensor provides a new and simple means for the ultrasensitive determination of microcystins in real water samples.

Objective To evaluate the effects of CHOICE management mode on adherence of adolescents with type 1 diabetes mellitus (T1DM). Methods A total of 49 adolescents with T1DM were elected using convenience sampling method, and divided into the experimental group (26 cases) and the control group (23 cases) by random number table. The experimental group received the CHOICE management for 1 year, while the control group received traditional education management. The patients′self-management behaviors and HbA1c were evaluated before the intervention and 1, 3, 6 and 12 months after the intervention. Results The scores of Control Problems, Attitude Toward Diabetes and Understanding Management Knowledge after 12-months intervention were (2.71 ± 0.43), (3.89 ± 0.60), (3.05 ± 0.71)points in the experimental group, (2.49 ± 0.33),(4.20 ± 0.40),(3.51 ± 0.70)points in the control group, there were significant differences (t=-2.101, 2.129, 2.289, P<0.05). The scores of Diet Adherence after 1 month and 3 months intervention were (2.85 ± 0.0.67), (2.79 ± 0.66)points in the experimental group, (2.39 ± 0.70), (2.38 ± 0.67)points in the control group, there were significant differences (t=2.170, 2.338, P<0.05). HbA1c was(8.29 ± 0.76)％ in the experimental group, (8.85 ± 1.14)％ in the control group, there was significant difference (t=-2.041, P<0.05). There were no significant differences at the others times between two groups (t=- 1.556-- 0.527, P>0.05). Conclusions CHOICE management mode can effectively improve the self-management behavior of adolescents with T1DM.