Objective To find the nursing methods to train the neonatal sucking function and accelerate the gastrointestinal peristalsis and the secretion of digestive juice. Methods 80 premature infants with feeding difficulty within two days after they were born were chosen by random. Then according to the psychological characteristics of neonates, took the canonical and practical feeding methods by the appointed person in continuity. Results Compared with before, the incidence of difficult feeding decreased, sucking ability and gastrointestinal function greatly alleviated after using the modified method. Conclusions The improved method could accelerate the recovery of sucking and gastrointestinal function as soon as possible for premature infants without complications, which has practical clinical significance.

Objective To investigate the effect of repetitive transcranial magnetic stimulation (rTMS) combined with eye-acupuncture on cognitive dysfunction on cognitive dysfunction in patients with traumatic brain injury. Methods From May, 2014 to June, 2016, 90 trau-matic brain injury patients with cognitive impairment were randomly divided into eye-acupuncture group (n=30), rTMS group (n=30) and combination group (n=30), who accepted eye-acupuncture, rTMS and combination of eye-acupuncture and rTMS, repectively, for eight weeks. They were assessed with Loewenstein Occupational Therapy Cognitive Assessment (LOTCA) before and after treatment. Results There was no significant difference in scores of LOTCA among three groups before treatment (F<0.523, P>0.05). The scores of LOTCA were the best in the combination group after treatment (F>3.789, P<0.05). Conclusion The combination of eye-acupuncture and rTMS is more effective in improving cognitive function after traumatic brain injury than either alone.

To investigate the protective effects of scutellarin benzyl ester on neonatal rats' cardiomyocytes injured by ischemia and its anti-apoptosis mechanism.

Objective To investigate the characteristic of regional amplitude of low frequency fluctuation (ALFF) in the brain of adolescents with online game addiction during the resting state.Methods The ALFF method was employed to explore the local features of spontaneous brain activity in adolescents with online game addiction (n =26) and healthy controls (n =23) during resting-state by Resting-State fMRI Data Analysis Toolkit.Results Compared with healthy controls,adolescents with online game addiction showed increased ALFF values in the anterior cingulate cortex (ACC),left middle cingulate cortex (MCC),bilateral caudate,right pallidum,bilateral parahippocampal gyrus,and right insula.It was noteworthy that the ALFF values of the right caudate (r =0.4437,P =0.0232) and right parabippocampal gyrus (r =0.5227,P =0.0062) were positively correlated with the duration of online game addiction.Conclusion The abnormal spontaneous neuronal activity properties during the resting state were revealed in the brain regions of adolescents with online game addiction.And the abnormalities may become more significant as the disease progresses.

Objective To observe any effect when eye acupuncture is combined with computer-assisted cognition training to ameliorate cognitive dysfunction after stroke.Methods Stroke patients with cognitive impairment were randomly divided into an eye acupuncture group (n=30),a computer group (n=30) and a combination group (n=30).The eye acupuncture group was given eye acupuncture,the computer group was given rehabilitative cognition training with specialized equipment and the combination group was given both eye acupuncture and the computeraided training.The treatment lasted 2 months.The Loewenstein occupational therapy cognitive asessment (LOTCA)and the modified Barthel index were used to evaluate the patients' cognitive function and daily life ability before and after treatment.Results Before training there was no significant difference in average LOTCA and MBI results among the three groups.After 2 months of training,all three groups had significantly higher scores in both evaluations.Moreover,the combination group's average score on organization ability,perceptual ability,thinking operation ability and concentration,and also their average total score and MBI score were significantly better than those of the other two groups.Conclusions Eye acupuncture combines synergistically with cognitive rehabilitation training to ameliorate cognitive dysfunction after stroke.Together they promote cognition,ADL ability and an early return to normal family and social life better than either alone.

Objective To investigate the epidemiological characteristics of gonorrhea,and to analyze its temporal-spatial clustering in Zhejiang province.Methods Data on the incidence and demographic characteristics of gonorrhea in Zhejiang province from January 2004 to December 2012 were obtained from the China Information System for Disease Control and Prevention.The population,time and space distributions of gonorrhea were described.Epidemic curve and incidence maps were drawn.A space-time permutation scan statistic was used to detect space-time clusters,and spatial autocorrelation analysis was performed to calculate the Moran's I value and draw Local Indicators of Spatial Association (LISA) cluster maps.Results In Zhejiang province,a total of 199 956 cases of gonorrhea were reported with a decreasing trend in incidence rate from 2004 to 2012.The male to female ratio was 3.51:1 (155 634/44 331).People aged between 25 and 60 years accounted for 75.21％ of these patients,whereas the constituent ratio of people aged 0-1 years and ＞ 60 years increased with time.The incidence rate of gonorrhea was significantly higher in middle and north parts than in the south part of Zhejiang province,and higher in summer than in winter and spring with the peak incidence observed in August.Thirteen temporal-spatial clusters were detected,with the large clusters in Hangzhou,Huzhou,Ningbo,Shaoxing and their neighbor counties/cities/ districts,as well as some counties/cities/districts in Jinhua.All of the above clusters lasted 4.5 years.LISA maps showed an increasing trend in high-high aggregation counties/cities/districts which spread from the north to south part of Zhejiang province.Conclusion There is a temporal-spatial aggregation of gonorrhea in Zhejiang province with young and middle-aged men as the main affected population.

Objective To develop a novel anti-Enterovirus 71 (EV71) vaccine as recombinant virus-like particles.Methods By utilizing the foreign antigen presentation and virus-like particles forming features of Norovirus casipid VP1 P domain (NoVP), two pET-28a (+)-based recombinant expression plasmids containing either NoVP alone or NoVP with three specific epitopes SP55, SP70 and VP2-28 of EV71 capsid proteins tandemly inserted at the surface loop site were constructed and transferred to Escherichia coli.The recombinant fusion proteins of NoVP + EV71-SP55-SP70-VP2-28 and NoVP were induced expression and confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), Western blot and transmission electron microscopy (TEM) observation.BALB/C mice were randomly divided into three groups:group A immunized with the recombinant fusion protein, group B immunized with NoVP and group C injected with 10 mmol/L Tris plus 20 mmol/L NaCl (pH 9.0).Enzymelinked immunosorbent assay (ELISA) was used to test specific antibodies in the serum of the mice, besides, the serums were mixed with the EV71 H3-TY strain and Vero cells, then specific antibody titer was examined by microneutralization test.One way ANOVA and Bonferroni test were used to analyze data.Results Both recombinant fusion protein and NoVP were expressed in Escherichia coli in inclusion bodies form.SDS-PAGE demonstrated that the relative molecular weights of recombinant fusion protein and NoVP protein were approximately 43 × 103 and 36 × 103, respectively;positive protein band of about 43 × 103 (relative molecular mass) was detected in recombinant fusion protein by Western Blot.Virus-like particles derived from the recombinant fusion proteins were observed under TEM.ELISA showed that absorbance 490 (A490) of mice serum added in SP70 peptide was significantly higher than those of group B and C (F =13.860,P ＜0.05).And microneutralization test demonstrated that the serum from group A was able to neutralize EV71 at a geometric mean titer above 1:38.Conclusion A novel virus-like particles vaccine against EV71 with good antigenicity and specificity has been prepared, which is able to induce high titer of neutralizing antibody against EV71 in mice.

ObjectiveTo study the effect of consolidated trunk muscle training on balance and motor function in patients with hemiplegia after stroke. Methods96 patients were divided into treatment group (48 cases) and control group (48 cases). All the patients received regularly rehabilitation, and the patients in the treatment group received the control ability training in the trunk muscle in addition. They were assessed with Trunk Control Test (TCT)， Berg Balance Scale (BBS)， Modified Barthel Index (MBI), Functional Ambulation Category (FAC), Fugl-Meyer Assessment (FMA) and walking speed (WS) before and after the treatment. ResultsThe scores of TCT, BBS, FAC, MBI, WS and FMA improved significantly in both groups after treatment (P<0.01)， and improved more in the treatment group than in control group (P<0.05). The scores of TCT correlated with those of BBS, MBI, FAC, FMA, and WS (P<0.05). ConclusionThe consolidated trunk muscle training can obviously improve balance and motor function of stroke patients.

Objective To observe the effect of optimized nursing procedure on the emer-gency treatment of patients with acute myocardial infarction(AMI).Methods A total of 35 AMI patients in the observation group implemented optimized nursing procedure to conduct rescue and nursing.Delicacy Management was optimized and the nurses were conducted optimized nursing procedure.Afterwards,it was continuously improved according to the efficacy evaluation.And du-ration of stay in emergency department,satisfaction of patients towards nurses as well as satisfac-tion of doctors towards nurses in the control group implemented traditional nursing process with 35 patients and the observation group were analyzed.Results Length of emergency stay of the obser-vation group was significantly shorter than that in the control group and the doctors’satisfaction towards nurses was significantly higher than that in the control group(P <0.05).Conclusion Optimized nursing procedure in emergency treatment of AMI patients can standardize nursing and improve the nursing efficiency and nursing quality.

Objective To observe the effect of optimized nursing procedure on the emer-gency treatment of patients with acute myocardial infarction(AMI).Methods A total of 35 AMI patients in the observation group implemented optimized nursing procedure to conduct rescue and nursing.Delicacy Management was optimized and the nurses were conducted optimized nursing procedure.Afterwards,it was continuously improved according to the efficacy evaluation.And du-ration of stay in emergency department,satisfaction of patients towards nurses as well as satisfac-tion of doctors towards nurses in the control group implemented traditional nursing process with 35 patients and the observation group were analyzed.Results Length of emergency stay of the obser-vation group was significantly shorter than that in the control group and the doctors’satisfaction towards nurses was significantly higher than that in the control group(P <0.05).Conclusion Optimized nursing procedure in emergency treatment of AMI patients can standardize nursing and improve the nursing efficiency and nursing quality.