1.First molecular genotyping of A302S mutation in the gamma aminobutyric acid (GABA) receptor in Aedes albopictus from Malaysia
Tropical Biomedicine 2015;32(3):554-556
Given the lack of molecular evidence in altered target-site insecticide resistance
mechanism in Aedes albopictus (Skuse) worldwide, the present study aims to detect the
presence of A302S mutation in the gene encoding the gamma aminobutyric acid receptor
resistant to dieldrin (Rdl) in Ae. albopictus for the first time from its native range of South East
Asia, namely Malaysia. World Health Organization (WHO) adult susceptibility bioassay indicated
a relatively low level of dieldrin resistance (two-fold) in Ae. albopictus from Petaling Jaya,
Selangor. However, PCR-RFLP and direct sequencing methods revealed the presence of the
A302S mutation with the predomination of heterozygous genotype (40 out of 82 individuals),
followed by the resistant genotype with 11 individuals. This study represents the first fieldevolved
instance of A302S mutation in Malaysian insect species.
2.Rapid genotyping of Plasmodium vivax Pvs25 and Pv38 genes by using mismatch specific endonuclease
Jang, J.W. ; Cho, C.H. ; Kim, J.Y. ; Koh, Y.E. ; Woo, M.K. ; Kim, K.A. ; Yoon, S.Y. ; Lim, M.S. ; Han, E.T. ; An, S.S.A. ; Lim, C.S.
Tropical Biomedicine 2014;31(4):600-606
Mismatch specific endonuclease (MSE) method was used to detect natural
polymorphisms in Pvs25 and Pv38 genes of Plasmodium vivax. Eighty seven patients with P.
vivax were recruited in the Republic of Korea (ROK). Pvs25 and Pv38 genes were amplified
by polymerase chain reaction (PCR), and the PCR amplicons were mixed with reference DNA
sequences. Following the denaturation and gradual annealing, the product mixtures were
cleaved by the MSE. Heteroduplex types were readily detected by gel electrophoresis, where
extra bands with shorter sizes would appear from the cleavage. After MSE cleavage of 657-
bp product from Pvs25 mixtures, three genotypes were detected, while Pv38 mixtures with
1220-bp products presented two genotypes in ROK isolates. After the MSE cleavage, the
mismatched samples of Pvs25 and Pv38 were completely sequenced, and the results were in
complete agreement with the MSE analyses. In conclusion, genotyping of Pvs25 and Pv38
with MSE cleavage could be a potential method for the high-throughput screening of the large
field samples.
3.Imported case of Leishmania tropica cutaneous leishmaniasis in a 10-year-old child in Malaysia
Tan, T.K. ; Yap, N.J. ; Leong, K.F. ; Teh, C.S. ; Tay, S.T. ; Lim, Y.A.L.
Tropical Biomedicine 2022;39(No.1):86-88
The present paper reported a first imported case of cutaneous leishmaniasis in a 10-yearold child who returned from Saudi Arabia to Malaysia. Six weeks after his travel to Malaysia,
two erythematous dermal nodules were developed over his right cheek and chin. Occurrence
of intracellular amastigote of Leishmania was observed through examination of skin biopsy
with hematoxylin and eosin stain. Furthermore, molecular analysis of ribosomal internal
transcribed spacer 1 (ITS1) of Leishmania spp. confirmed the child was infected with Leishmania
tropica. The child was given oral fluconazole and he had a 80% recovery before he went back
to Saudi Arabia.
4.A TaqMan minor groove binder probe-based quantitative reverse transcription polymerase chain reaction for detection and quantification of chikungunya virus
Lim, Y.Z. ; Teoh, B.T. ; Sam, S.S. ; Azizan, N.S. ; Khor, C.S. ; Nor&rsquo ; e, S.S. ; Abd-Jamil, J. ; AbuBakar, S.
Tropical Biomedicine 2023;40(No.3):313-319
5.Development of a TaqMan minor groove binding probe-based quantitative reverse transcription polymerase chain reaction for the detection and quantification of Zika virus
Chin, K.L. ; Teoh, B.T. ; Sam, S.S. ; Loong, S.K. ; Tan, K.K. ; Azizan, N.S. ; Lim, Y.K. ; Khor, C.S. ; Nor&rsquo ; e, S.S. ; Abd-Jamil, J. ; AbuBakar, S.
Tropical Biomedicine 2022;39(No.4):518-523