BACKGROUND: It is well known that central noradrenergic system takes part in learning and memory, but there are a few researches focused on the effects of impairing the central noradrenergic system on rat' s P3-like cognitive potential.OBJECTIVE: To study the effects of impairing the central noradrenergic system on rat's P3-like cognitive potential.DESIGN: Randomized controlled study based on the experimental animals.SETTING: Department of physiology in a medical college of a university.MATERIALS: Totally 30 male SD rats, clean degree, weighing from 500 to 600 g, were selected from the Experimental Animal Center of Sun Yat-sen Medical University(certificate: 26-99A018), drank freely and fed timely under the normal condition. After Y-maze test of all the rats, 24 rats with normal learning and memory function, which met the criteria specified in the description of Y-maze test, were randomly divided into three groups(8 rats in each group): control group, 6-oxidopamine (6-OHDA) injection group and saline control group.INTERVENTIONS: The models of central norndrenergic system impairment were made by injecting 6-hydroxysopamine(6-OHDA) into the bilateral dorsal noradrenergic bundle in dentate granule(DG). Then Y-type maze test and elicitation of P3-likewere carried out respectively before and after the models were built.MAIN OUTCOME MEASURES: Main outcome: P3-like latency, total reaction time(TRT) and error number(EN). Secondary outcome: Results of P3-like test recorded in the 12 days after the models were built.RESULTS: As compared with the controls, the model rats' P3-like latency and total reaction time(TRT) prolonged and error number(EN) increased statistically ( P ＜ 0.05 ).CONCLUSION: Central NE takes part in the activities of learning and memory and plays a certain role in the production and integration of P3 -like.

Objective:To investigate the clinical effect of enteroclysis of Chinese crude drug in treating different stages of chronic renal failure(CRF).Methods:Forty one inpatients with CRF in the nephrology were summarized,who had taken on the treatment with enteroclysis.At the end of the treatment,the change of symptoms and BUN,Scr,Ccr of the inpatients with CRF were recorded and statistically analyzed retrospectiveiy.Results:The total effective rate of enteroclysis in treating CRF was 58.54%,the patients whose Ccr was at the stage of 20-10ml/min reached 75.00%,while Scr,BUN decreased signifi cantly and Ccr rose markedly(P

Objective To explore the clinical effect of mifepristone combined with misoprostol for termina-tion of pregnancy in high risk pregnant women and provide reference for clinical treatment.Methods 182 cases of high -risk maternal were divided into observation group and control group by the random number table method,each group 91 cases.The control group was treated with conventional abortion termination of pregnancy,the observation group was treated with the artificial abortion before oral administration of mifepristone tablets and misoprostol tablets. The operation time,the amount of blood loss,the degree of pain,the degree of cervical softening and the occurrence of complications of induced abortion were compared between the two groups.Results The operation time of the observa-tion group was (2.21 ±1.41)min,intraoperative bleeding was (15.29 ±4.06)mL,which were significantly less than those of the control group (t =7.122,8.256,all P <0.05);The pain degree grade I in the observation group was 58.24%,which was significantly higher than that in the control group,grade III accounted for 5.49%,which was sig-nificantly lower than the that in the control group (χ2 =44.887,44.121,all P <0.05),the observation group of cer-vical softening full was 59 cases,full softening rate was 64.84% and the control group in the cervical softening full was 41 cases,softening full rate was 45.05%.Fully softening rate in the observation group was significantly higher than that in the control group (χ2 =7.191,8.411,P <0.05),the control group,the incidence of uterine perforation in 2 cases,the occurrence of artificial abortion syndrome 7 cases,the incidence of complications for 9.89%,the obser-vation group had no uterine perforation occurred,1 case of artificial abortion syndrome and complications occurred rate was 1.10%.The incidence of complications in the observation group was significantly lower than that in the control group (χ2 =6.772,P <0.05).Conclusion Mifepristone combined with misoprostol for high -risk maternal termi-nation of pregnancy could significantly shorten the artificial abortion operation time,reduce intraoperative blood loss, reduce the patient pain,reduce the incidence of complications.

OBJECTIVE:To study the infections induced by ureaplasma urealyticum(UU) and mycoplasma hominis(MH) in Huangshi city and their antibiotic resistance for reference of clinical rational drug use.METHODS:UU and MH were detected by mycoplasma rapid culture and drug sensitivity test kit.RESULTS:Of the total 350 patients who were suspected as having nongonococcal urethritis(NGU),152(43.43%) were positive in mycoplasma detection.52 of the total 146 male cases were positive(35.62%),and 100 among the 204 female cases were positive(49.02%),with positive detection rate significantly higher than in male case(?2=6.222,P

The phenotype frequencies of Gc and Tf in blood and bloodstains from the Hannationality in Bejing was studied using the agarose gel simultaneous electrophresisfollowed by the immunofixation method.Their gene frequencies were calculated.

Objective To study the expression of dihydrodiol dehydrogenase (DD) in prostate cancer and its significance. Methods With reference to expression of ?-actin gene,the expression level of a human dihydrodiol dehydrogenase isoform (DD2) mRNA was examined in prostate cancer tissues (11 cases) and normal prostate tissues (10 cases) by reverse transcriptive-polymerase chain reaction(RT-PCR). Quantitative determination of relevant band densities was performed using densitometry-scanning techniques. Results Strong expression of DD2 mRNA was detected in prostate cancer tissures with absorbance in the range of 0.550 to 1.018 (median,0.726),and low expression of DD2 mRNA in normal prostate tissues with absorbance in the range of 0.248 to 0.420 (median,0.333). The difference of the expression of DD2 mRNA between cancer and normal prostate was significant ( P

Objective Effects of exercise training and Chinese Medicine Compound for Invigorating Qi and Tonifying the Kidney on testicular StAR and P450 SCC mRNA expression in rats were observed. Methods 50 Wistar rats were randomly divided into 4 groups: sedentary control group(SC, n=10), sedentary with medicine group (SM,n=10), training group(T, n=15) and training with medicine group(TM, n=15). After 6 weeks swimming training , serum testosterone level of rats were tested through radioimmunological method; using RT-PCR, we measured testicular StAR and P450 SCC mRNA levels of rats. Results (1) In T group, serum testosterone level was lower than that in SM and TM groups. Whereas, compared with SC group, the serum testosterone level in TM group kept steadiness. (2) In T group, the gene expression of StAR was markedly lower than that in SC control (P

0.05) after 6-week training. Conclusion The results indicated that supplementation with ABPS or APS may help regulate cellular immune capacity through modulating IL-2 and its receptors levels in rats during strenuous training.

Objective To study the expression and significance of antiapoptosis facter-clusterin in prostate neoplasm. Methods Reverse transcriptive polymerase chain reaction(RT PCR) was used to examine the expression level of clusterin in prostate cancer tissues(3 cases),prostate cancer cell line PC3M and normal prostate tissues(3 cases). Results Compared with the internal marker gene ? actin , the expression level of clusterin in prostate cancer is much higher than that of normal prostate. Conclusions The high expression level of clusterin in prostate cancer indicates that clusterin may play an important role in the biological characteristics of prostate cancer through the antiapoptosis pathway.

Objective:To analyze immune mechanisms of total glycoside from Cornus officinalis (TGCO) against rheumatoid arthritis.Methods:Rat immunized by CⅡ in CFA were treated with TGCO ,at different times after immunization,CⅡ-specific antibodies in serum,lymphnode cells(LNC) IFN-? production was studied and cell proliferation response were also detected.Results:Treatment inhibit disease severity to a greater extent. TGCO effectively decreased anti-CⅡantibody titer in serum, considerably reduced IFN-? secretion before the onset arthritis and the acute stage of arthritis and suppressed T cell differentiation or proliferation.Conclusion:The both lead to supprese arthritis.