Objective To explore the influence of health education on the cognition and behavior of oc-cupational protection for nurses in operation room. Methods 85 nurses in operation rooms from 2 compre-hensive hospitals were selected with random clustering methods, baseline surveys and health education about the cognition and behavior of occupational protection were carded out in them. Results The awareness rate of occupational protection knowledge after health education was higher than that before it. The behavior of occupa-tional protection was better than that of before health education. Conclusions Health education can advance cognition and ability of occupational protection, then can prevent occupational risks effectively.

Objective To explore the effects of mindful- based stress reduction exercise on the sleep and negative emotion in Primary glaucoma patients. Methods By using random sampling,primary glaucoma patients at the hospital of between September in 2015 and September in 2016. A total of 93 patients were divided into intervention group and control group according to random number table.The control group was given conventional nursing, intervention group joined in mindful-based stress reduction exercise on the basis of routine nursing. Baseline levels of anxiety, depression and status of sleep were measured in the two groups after admission, evaluated and comparison at the end of formal intervention. Results At the end of intervention, the score of Hamilton Anxiety Scale (HAMA) in intervention group was decreased from (13.42 ± 3.56) points to (8.23 ± 2.11) points (t=7.832, P=0.000), the score of Hamilton Depression Scale (HAMD) was decreased from (18.42 ± 4.55) points to (10.31 ± 2.76) points (t=9.517, P=0.000), the effect was better than the control group (t=8.117, 7.671, all P=0.000). And the score of Self-Rating Scale of Sleep (SRSS) was decreased from (30.81±8.35) points to(25.52±6.13) points (t=3.189, P=0.002), the effect was better than the control group (t=2.253, P=0.027). Conclusions Mindful-based stress reduction exercise can reduce negative emotions such as anxiety and depression and promote the status of sleep effectively.

Anticipatory nausea and vomiting (ANV) is one of the common adverse effects in cancer patients undergoing chemotherapy.ANV is related to classical conditioning,psychosocial and demographic factors.Psychological intervention such as systematic desensitization,hypnosis and biofeedback therapy could be used to treat ANV,while some psychotropic medication including benzodiazepines and olanzapine is also helpful to prevent ANV.Now the recognition about ANV in clinical oncology is not adequate and large sample epidemiological survey of ANV is lacked.So far there are no special tools to predict and assess ANV,and the effects of pharmacological interventions also need solid evidence from large sample double-blind randomized controlled trials.

Objective To explore the short-term and long-term clinical effect of leep knife treatment of chronic cervicitis. Methods A single-center, prospective, randomized, controlled study was used, 120 patients with chronic cervicitis were randomly divided into observation group and control group, each group of 60 patients. Patients in the observation group were treated with (LEEP) knife, parameters setting frequency was 3.8 MHz, power was 30 ~ 40W, patients in the control group were treated with microwave. The intraoperative and postoperative recovery and relapse were compared between two groups. Results The intraoperative blood loss, operative time, postoperative vaginal discharge time in the observation group were significantly less than control group, there was a significantly statistical difference (P<0.001) . After two weeks and three months , the cure rate in observation group were 70% and 98.33% respectively, the cure rate in control group were 16.67% and 51.67%, there was a significantly statistical difference (P <0.01) . After 2 weeks and 3 months, the infection rate and recurrence rate in the observation group were significantly lower than the control group, there was a significantly statistical difference (P <0.05) . Conclusion Leep knife for treatment of chronic cervicitis patients has lower postoperative recurrence, lower infection rate and higher cure rate in short-term and long-term, and less intraoperative trauma, shorter operation time, and has important clinical significance for the prognosis of patients.

Objective:To determine if a group psychotherapy programme offers significant benefits to psycho-logical wellbeing for early and mid-term colorectal cancer patients compared to control group.Methods:Totally 67 patients previously diagnosed with colorectal cancer who participated in this study,34 patients accepted an invitation to join the intervention group,received 10 consecutive,weekly sessions group psychotherapy,and 33 patients served as control subject with usual care only.All participants were assessed at baseline and post-therapy (week 10 follow-ing enrollment or equivalent ).Assessment comprised self-completed Cancer Quality-of-life Core Questionnaire (QLQ-C30),Self-Rating Depression Scale (SDS)and Self-Rating Anxiety Scale (SAS).Results:At baseline, 67participants completed questionnaires,and 60 questionnaires returned at post-therapy,from 30 intervention group participants and 30 control group participants.Baseline scores for SAS and SDS were higher in intervention than control group[(42.0 ±7.7)vs.(36.0 ±6.8),(44.4 ±11.5)vs. (38.7 ±9.0),Ps <0.05],while other itemswere not differ(Ps>0.05 ).Post-therapy,intervention group participants reported significantly scores for anxiety, depression and fatigue compared with controlled group.In the comparison of before-post difference between inter-vention and controlled group,the difference of SAS score,SDS score,role function score and fatigue score showed significant discrepancy,intervention group improve significantly (Ps <0.05 ).Conclusion:Group psychotherapy could improve anxiety and depression,for colorectal cancer patients,and may help to improve quality of life,possi-bly through improving role function and decreasing fatigue symptoms.

By analyzing the problems existing in the practice of doctor -patient communication , respectively discusses the speech act theory before the implementation of the implementation of doctor -patient communication oral English training , implementation , and after implementation of the positive role: instant help medical students to master a large number of medical English vocabulary;In view of the students come from different areas , instant help correct the pronunciation and intonation;Benefit based on speech act theory to guide the student to carry on the different roles played in English oral practice helps students to adopt direct and indirect speech act theory and the method of the oral English practice for communication;To improve medical students′professional recognition;To strengthen the medical students′sense of mission .

As the global economic integration, the trend of internationalization of medical education, social demand for foreign talents is more and more big, the English teaching, especially the doctor-patient communication in English teaching has become a major focus on the teaching content.Learning is the study of doctor-patient communication be-tween medical workers and patients how to mutual understanding, trust and cooperation, common a discipline, to conquer the disease and emphasize ability oriented ESP teaching concept, closely integrated with professional, promotes the student as the center, apply English teaching as means and tools to study, the two organically unifies in together.Practice shows that guided by the ESP teaching concept, improve medical students English power of expression of doctor-patient commu-nication attempt made substantial results.

Objective To study the prognosis of patients with unstable angina in PCI adverse events factor analysis,and the function of CYP2C 19 gene mutations.Methods From May 2013 to May 2015,226 cases of PCI postoperative patients,after surgery all took aspirin + clopidogrel maintenance to treat for 1 years,analyzed CYP2C19*1,CYP2C19*2,CYP2C19 *3 genotype with fluorescent polymerase chain reaction (PCR),the patients were followed up for 6 months.According to the result of detection of genotype divided into wild type group (CYP2C19 *1/*1),the mutation of mixed group (CYP2C19 *1/*2 and CYP2C19 *1/*3) group and mutations in pure form group (CYP2C19 *2/*2 and CYP2C19 *3/*3).Observed the occurrence of unstable angina of three groups prognosis.Results The mutation of mixed to form and mutation of pure form had a record of hospital clinical adverse events (61.9％ and 45.3％) was significantly higher risk of wild group (42.4％),with statistical significance (P ＜0.05);Logistic regression analysis of diabetes and CYP2C19 genotype with PCI postoperative patients with unstable angina occurred in hospital records of clinical adverse events had good correlation (R =3.424,P=0.009 and R =5.679,P =0.001).Conclusion The CYP2C19 gene mutations and diabetes patients with unstable angina PCI postoperative prognosis is closely related,the lack of CYP2C19 gene type are more likely to affect the prognosis of unstable angina,provide reliable basis for further research.

Objective To investigate the influence of different treatments for tubal pregnancy on ovarian reserve function by transvaginal color Doppler ultrasonography.Methods One hundred and twentyfour cases of tubal pregnancy were divided into three groups according to the treatment,group A with 45 cases under the salpingectomy,group B with 36 cases under the laparoscopic conservative surgery for retaining oviduct,group C with 43 cases taken by Methotrexate.On the day of a spontaneous cycle,all the cases' antral follicles,the ovarian volume,peak systolic velocity (PSV),resistance index (RI),pulsatility index(PI) were measured by transvaginal color Doppler ultrasound.Results The antral follicles,varian volume and PSV of diseased side in group A were lower than those of the other side(P ＜0.05).The antral follicles,varian volume and PSV of diseased side in group A were lower than those of other groups (P ＜0.001).The PI and RI of diseased side in group A were higher than those of the unaffected side.The PI and RI of two sides in group B and C were not significantly different(P ＞0.05).Conclusions Salpingectomy affects the ovarian reserve function.Methotrexate and conservative surgery could not reduce the ovarion reserve.

Objective To construct the eukaryotic expression vector of pprI gene from Deinococcus radiodurans R1 and investigate its radioresistant effects in eukaryotic cells.Methods A recombinant vector pEGFP-c1-pprI was constructed by DNA recombinant technique.The empty vector pEGFP-c1 and the pEGFP-c1-pprI were transferred into human lung epithelial cells Beas-2B by LipofectamineTM 2000,respectively.Then the infected cells were screened in order to develop a cell line with stable expression of pprI gene.Cell survival rate was tested by clone-forming assay.Cell cycle distribution and apoptosis were detected by a flow cytometry.The fluorescence intensity of reactive oxygen species (ROS) was observed by a fluorescent microscope.γ-H2AX foci in the irradiated cell was detected by immunofluorescence.Results The eukaryotic expression plasmid of pprI prokaryotic gene was constructed and PprI fusion protein was expressed in human lung epithelial cells successfully,and the cell line (2BG) with a stable pprI gene expression was established.After irradiation,the cell survival fraction of 2BG cells was significantly higher than Beas-2B cells so that the value of D0 、Dq and N of the survival curve were increased.Moreover,the fluorescence intensity of ROS and the number of γ-H2AX foci in 2BG cells were also lower than those of B eas-2B cells(F =16.73,19.47,6.94,P ＜ 0.05).Between these two cell lines,the apoptosis rate and cell cycle G2 arrest also had significant difference (F =139.73,237.92,P ＜ 0.05).Conclusions The pprI gene from Deinococcus radiodurans RI can be stably expressed in the eukaryotic cells and it allows the transferred cells to have a radioresistant function.