Objective:To study effect and mechanism of Anchang Yuyang Decoction with Jiawei Shengji Powder (ACYYD&JWSJP) on ulcerative colitis (UC) in rats. Methods: Ninety SD rats were divided randomly into six groups: normal control group,pathologic model control group,ACYYD group, JWSJP group, ACYYD & JWSJP group And Solfasalazine (SASP) treatment group. The model of ulcerative colitis were established by given 2,4,6-TNBS. After medication for 3 weeks, the parameters including colonic gross morphology, colon tissue injury scores, MPO activity, the content of IL-1?, IL-8, TNF-? and IL-10 were observed. Results: Inflammation relieving of colonic mucosa and ulcer healing were found in all treatment group rats at different degree. Colonic gross morphology, colon tissue injury scores,MPO activity and the level of IL-1?,IL-8, TNF-? decreased, while levels of IL-10 increased after therapy (P

Objective To investigate the effect of acetylcysteine on the inflammation and oxidative stress in elderly patients with chronic obstructive pulmonary disease(COPD).Methods Sixty patients with COPD were divided into two groups by random mechanical sampling method (30 cases each group).The control group was treated with conventional therapy and the treatment group added acetylcysteine for 8 weeks.The levels of tumor necrosis factor- t (TNF- α ),interleukin-6 (IL-6),interleukin -8 (IL-8),superoxide dismutase (SOD),malondialdehyde (MDA) were monitored and analyzed before and after treatment.Results The levels of SOD,MDA were (51.08 ± 7.80),(75.09 ± 8.03) μ U/L and (7.13 ± 0.89),(4.51 ±0.61 ) μ mol/L before and after treatment in the treatment group,and (52.12 ± 7.31 ),(65.16 ± 8.01 ) μ U/Land (7.11 ± 0.87),(6.21 ± 0.78 ) μ mol/L in the control group.There were significant differences before and after treatment in two groups (P＜0.01 or ＜0.05),and there were significant differences after treatment between two groups (P ＜ 0.05).The levels of IL-6,IL-8 and TNF- α were significant differences before and after treatment in the treatment group [ ( 18.25 ± 7.24) ng/L vs.(29.02 ± 6.70) Ng/L,( 115.28 ± 13.76 ) ng/Lvs.(148.99 ± 16.61) ng/L,(20.43 ±3.92) ng/L vs.(32.32 ± 8.18)ng/L] (P ＜0.05).But there was nosignificant difference before and aftertreatment in the control group [ (25.25 ± 7.55 )ng/L vs.(28.82 ± 7.54)ng/L,( 136.27 ± 12.97) ng/L vs.( 150.21 ± 17.52) ng/L,(28.43 ± 3.92) ng/L vs.(32.56 ± 8.78) ng/L] (P＞0.05),there were significant differences after treatment between two groups (P ＜0.05).Conclusions Acetylcysteine can inhibit inflammation and e hminate free radicals in plasma.Acetylcysteine has beneficial effects on COPD.

Objective:To analyze the monitoring results of high resolution esophageal manometry (HRM) and 24-Hour impedance-pH for the patients with functional heartburn (FH)and non-erosive reflux disease(NERD), and explore the effect of Gastroesophageal junction morphology, transient lower esophageal sphincter relaxation and regurgitation of gastric acid in FH and NERD.Methods: 86 patients with continuous heartburn who were negative under endoscope were divided into FH group(38cases) and NERD group(48cases), and gastrointestinal symptom rating scales (GSRS), the results of HRM and 24h pH/impedance monitoring of the two groups were compared and analyzed.Results: The GSRS of sour regurgitation, abdominal distension, diarrhea and constipation of FH group were significantly higher than that of NERD group, respectively(t=-4.45,t=3.83,t=3.07,t=4.58,P<0.05). The ratio of esophagogastric junction-III(EGJ-III)of FH group was significantly lower than that of NERD group (Z=-3.27,P<0.05). And the ratio of esophageal hiatus hernia of FH group was significantly lower than that of NERD group(x2=16.01,P<0.05). In HRM parameters, the differences of lower esophageal sphincter pressure (LESP), upper esophageal sphincter pressure(UESP), distal contractile integral(DCI)and esophagogastric junction- contractile integral (EGJ-CI) between FH group and NERD group were statistically significant(t=6.99,t=-4.51,t=-2.91,t=-3.72;P<0.05), respectively. On the other hand, the times of sour regurgitation in erect position, the ratio of fluid regurgitation, exposed time of erect position and clinostatism, and DeMeester integral of NERD group was statistically significant higher than that of FH group(t=-9.48,t=-13.00,t=-13.56,t=-18.31;P<0.05).Conclusion:There are esophageal dysfunction in a certain degree and obvious regurgitation in both of two groups, and the detections of HRM and 24h-impedance pH contribute to differential diagnosis for NERG. Besides, the symptom of patients with FH may be relative with regurgitation of non-acidic material, especially may be relative with regurgitation of air.

ObjectiveTo investigate the effect of moxibustion on the Governor Vessel in combination with acupuncture on ankylosing spondylitis.MethodsThirtypatetints with ankylosing spondylitis(kidneyyang deficiency pattern) were recruited and treated with moxibustion on the Governor Vessel in combination with acupuncture. The scores of Scale of Quality of Life for Ankylosing Spondylitis (SQOL-AS), Bath Ankylosing Spondylitis Disease Activity Index(BASDAI), Bath Ankylosing Spondylitis Functional Index(BASFI), Fatigue Assessment Instrument(FAI)and erythrocyte sedimentation rate(ESR) before and after treatment were evaluated.ResultsThe scores of BASDAI(0.427±0.381vs. 6.107±1.375;t=28.986,P<0.01), BASFI(0.753±0.504 vs. 7.480±1.152;t=36.580,P<0.01), FAI(40.067±14.746vs. 146.967±26.283;t=24.520,P<0.01)after treatment were significantly lower than those before treatment; ESR after treatment were significantly lower than that before treatment(19.833±13.929 mm/hvs. 31.567± 20.065 mm/h;t=3.708,P<0.01). The subscores of Physical Functioning(33.000±1.930vs. 12.033±1.790;t=62.165,P<0.01), Mental Health(25.367±2.059vs. 14.067±2.303;t=30.139,P<0.01), Social Functioning(21.267±4.025vs. 8.767±1.977;t=26.643,P<0.01) and General Health Perception (22.733± 2.690vs. 9.767±2.738;t=25.729,P<0.01) of SQOL-AS after treatment were significantly lower than that before treatment.ConclusionsMoxibustion on the Governor Vessel in combination with acupuncture can improve physical functioning and reduce disease activity in patients with ankylosing spondylitis.

BACKGROUND: Various types of collagen affect differentiation and expression of chondrocytes in the process of tissue engineered culture for cartilage. The reliability of cartilage tissue engineering is determined by resource, structure and property of collagen.OBJECTIVE: To characterizing the structure of collagen type Ⅰ,Ⅱ and Ⅲ, to clarify the structural characteristics of collagen for cartilage tissue repairing and provide guidance for choosing cartilage tissue engineering scaffolds. METHODS: The type Ⅰ and Ⅲ collagen was obtained from neonatal calf skins, and type Ⅱ collagen was obtained from porcine articular cartilage. The differences of structures of collagen type Ⅰ,Ⅱ and Ⅲ were analyzed and compared by FTIR, DSC, SDS-PAGE and picrosirius-red staining.RESULTS AND CONCLUSION: The neonatal calf collagen type Ⅰ,Ⅱ and porcine collagen type Ⅱ had the similar functional group. The thermal denaturation temperature of the three type collagen was 104.2 ℃, 99.7 ℃ and 92.5 ℃. SDS-PAGE analysis showed the collagen type Ⅲ percentage in collagen type Ⅰ was (11.29±0.91)%. Picrosirius-red staining exhibited that the collagen type Ⅰ was comprised red and orange raw fibers, with strongly birefringent. Collagen type Ⅲ was lax and thin, greenish fibers, but collagen type Ⅱ was red, loosely cancellated fibers. The functional groups and important characteristics of the three of collagen are similar. However, there are some differences in advanced structure. To analyze the relationships of collagen type Ⅰ,Ⅱ and Ⅲ is important to choose and prepare scaffolds for cartilage tissue engineering.

Objective To investigate the regulation on cell cycle related factor such as Cyclins and P21waf/cip1 by inhibiting histone deacetylase(HDAC)with valproic acid sodium(VPA).Methods HepG2 hep-tocellular carcinoma cells.BGC-823 gastric carcinoma cells and MCF-7 breast cancer cells were cultured with O.75-4.00 mmoL/L VPA for 48 h in vivo.and the cell cycle was analyzed by flow cytometry with PI assay.The protein and mRNA expression of Cyclin A,Cyclin D1,Cyclin E and P21waf/cip1 were analyzed by indirect immu-nofluorescence technique and RT-PCR.respectively.Results Compared with control groups,VPA at concen-trations 0.75-4.00 mmol/L exerted a significant inhibiting effect on G1 phase of HepG2,BGC-823 and MCF-7 cells(P<0.001).and the effect was dose dependent.Cyclin A was down-regulated both at mRNA and protein level in HepG2 and BGC-823 cells(P<0.001),but no difference in MCF-7 cells(P>0.05).Cyclin D1 was down-regulated both at mRNA and protein level(P<0.001)and P2lwaf/cip1 was up-regulated both at the mRNA and protein level in the three cell lines(P<0.001);Conversely,protein and mRNA expression of Cyclin E were unchanged upon treatment with VPA(P>0.05).Condusion Acetylization of histone intervened with VPA can regulate Cyclin D1 and P21waf/cip1 expressions obviously.To the expression of Cyclin A,it shows some difference according to the histogenesis and phenotypes of different carcinoma types.But there is not any obvious function on Cyclin E.Down-regulating Cychn D1 and up-regulating P21waf/cip1 may be the common target path-way in the inhibition of cell cycle G1 phase exerted by VPA.

OBJECTIVE To study and find reasonable methods of nursing care of incision through analyzing the risk factors in liver transplant patients.METHODS A total of 416 liver transplantation operations in our hospital from Sep 2004 to May 2006 were evaluated.The risk factors that resulted in the incision infection were considered.RESULTS The incision infection rate was decreased from 13.9% to 4.3% after improvement.CONCLUSIONS Nursing care is of great significance in preventing the incision infection in liver transplant patients.

Objective To study the application effect of multi-dimensional health education model in the outpatient department of gynecological painless surgery,and explore more standardized and more effective education method.Methods 2 639 patients were selected in Sir Run Run Shaw Hospital outpatient service of gynecological department from July 2010 to June 2011,and were randomly divided into the control group(1 302 cases) and the intervention group(1 337 cases).The control group adopted conventional health education,the intervention group adopted the multi-dimensional health education.Design form was used to analyze preoperative compliance of the two groups.Results The preoperative compliance of the intervention group was higher than those of the control group,the difference was statistically significant.Conclusions Multi-dimensional health education mode can improve the preoperative education compliance of patients in the outpatient service of department of gynecological painless surgery patients,reduce the rate of operation delay and anesthesia method alteration,reduce patient pain,increase peri-operative nursing quality of painless surgery.

ObjectiveTo investigate the distribution of types of human papillomavirus(HPV)in cervical cancer Xinjiang Uyghur women.MethodsFrom june 2008 to April 2010,patients in the gynecological departmen of people's Hospital of Xinjiang Uygur Autonomous Region,who was gidiagnosed cervical cancer and came from southern of Xinjiang.Cervical cells of these patients were collected,HPV genotyping DNA chip was used to detect HP-DNA of each collected sample.ResultsTotally 120 wommen were screened,the infecton rationof HPV was 95.8%(115/120).The positive rate of HPV-16 infection was 69.6%(80/115);other highrisk HPV infection rates were ranked from high to low as HPV-56,HPV-33,HPV-18,HPV-45,HPV-58,HPV-51,HPV-59,HPV-31,HPV-52,HPV-39,HPV-68,HPV-73,low-risk HPV infection rates were ranked from high to low as HPV-11,HPV-42,HPV-43,HPV-6.However,HPV-35,HPV-53,HPV-66,HPV83,HPV-MM4 and HPV-44 were not detected.Single infection of HPV16 or HPV56 was 53.9%(62/115)and 17.4(20/115)respectively,ultiple infection rate was 29.6%(34/115).ConclusionHPV-16 is the most common types in cervical cancer of Xinjiang Uyghur women.HPV56 maybe susceptible to the Xinjiang Uyghur women,reflecting the specificity of HPV infection in cervical cancer of Xinjiang Uygur women.It needs deep study to development a suitable HPV vaccines.