Objective:To study effect and mechanism of Anchang Yuyang Decoction with Jiawei Shengji Powder (ACYYD&JWSJP) on ulcerative colitis (UC) in rats. Methods: Ninety SD rats were divided randomly into six groups: normal control group,pathologic model control group,ACYYD group, JWSJP group, ACYYD & JWSJP group And Solfasalazine (SASP) treatment group. The model of ulcerative colitis were established by given 2,4,6-TNBS. After medication for 3 weeks, the parameters including colonic gross morphology, colon tissue injury scores, MPO activity, the content of IL-1?, IL-8, TNF-? and IL-10 were observed. Results: Inflammation relieving of colonic mucosa and ulcer healing were found in all treatment group rats at different degree. Colonic gross morphology, colon tissue injury scores,MPO activity and the level of IL-1?,IL-8, TNF-? decreased, while levels of IL-10 increased after therapy (P

ObjectiveTo investigate the effect of moxibustion on the Governor Vessel in combination with acupuncture on ankylosing spondylitis.MethodsThirtypatetints with ankylosing spondylitis(kidneyyang deficiency pattern) were recruited and treated with moxibustion on the Governor Vessel in combination with acupuncture. The scores of Scale of Quality of Life for Ankylosing Spondylitis (SQOL-AS), Bath Ankylosing Spondylitis Disease Activity Index(BASDAI), Bath Ankylosing Spondylitis Functional Index(BASFI), Fatigue Assessment Instrument(FAI)and erythrocyte sedimentation rate(ESR) before and after treatment were evaluated.ResultsThe scores of BASDAI(0.427±0.381vs. 6.107±1.375;t=28.986,P<0.01), BASFI(0.753±0.504 vs. 7.480±1.152;t=36.580,P<0.01), FAI(40.067±14.746vs. 146.967±26.283;t=24.520,P<0.01)after treatment were significantly lower than those before treatment; ESR after treatment were significantly lower than that before treatment(19.833±13.929 mm/hvs. 31.567± 20.065 mm/h;t=3.708,P<0.01). The subscores of Physical Functioning(33.000±1.930vs. 12.033±1.790;t=62.165,P<0.01), Mental Health(25.367±2.059vs. 14.067±2.303;t=30.139,P<0.01), Social Functioning(21.267±4.025vs. 8.767±1.977;t=26.643,P<0.01) and General Health Perception (22.733± 2.690vs. 9.767±2.738;t=25.729,P<0.01) of SQOL-AS after treatment were significantly lower than that before treatment.ConclusionsMoxibustion on the Governor Vessel in combination with acupuncture can improve physical functioning and reduce disease activity in patients with ankylosing spondylitis.

Objective:To analyze the monitoring results of high resolution esophageal manometry (HRM) and 24-Hour impedance-pH for the patients with functional heartburn (FH)and non-erosive reflux disease(NERD), and explore the effect of Gastroesophageal junction morphology, transient lower esophageal sphincter relaxation and regurgitation of gastric acid in FH and NERD.Methods: 86 patients with continuous heartburn who were negative under endoscope were divided into FH group(38cases) and NERD group(48cases), and gastrointestinal symptom rating scales (GSRS), the results of HRM and 24h pH/impedance monitoring of the two groups were compared and analyzed.Results: The GSRS of sour regurgitation, abdominal distension, diarrhea and constipation of FH group were significantly higher than that of NERD group, respectively(t=-4.45,t=3.83,t=3.07,t=4.58,P<0.05). The ratio of esophagogastric junction-III(EGJ-III)of FH group was significantly lower than that of NERD group (Z=-3.27,P<0.05). And the ratio of esophageal hiatus hernia of FH group was significantly lower than that of NERD group(x2=16.01,P<0.05). In HRM parameters, the differences of lower esophageal sphincter pressure (LESP), upper esophageal sphincter pressure(UESP), distal contractile integral(DCI)and esophagogastric junction- contractile integral (EGJ-CI) between FH group and NERD group were statistically significant(t=6.99,t=-4.51,t=-2.91,t=-3.72;P<0.05), respectively. On the other hand, the times of sour regurgitation in erect position, the ratio of fluid regurgitation, exposed time of erect position and clinostatism, and DeMeester integral of NERD group was statistically significant higher than that of FH group(t=-9.48,t=-13.00,t=-13.56,t=-18.31;P<0.05).Conclusion:There are esophageal dysfunction in a certain degree and obvious regurgitation in both of two groups, and the detections of HRM and 24h-impedance pH contribute to differential diagnosis for NERG. Besides, the symptom of patients with FH may be relative with regurgitation of non-acidic material, especially may be relative with regurgitation of air.

Objective To investigate the effect of acetylcysteine on the inflammation and oxidative stress in elderly patients with chronic obstructive pulmonary disease(COPD).Methods Sixty patients with COPD were divided into two groups by random mechanical sampling method (30 cases each group).The control group was treated with conventional therapy and the treatment group added acetylcysteine for 8 weeks.The levels of tumor necrosis factor- t (TNF- α ),interleukin-6 (IL-6),interleukin -8 (IL-8),superoxide dismutase (SOD),malondialdehyde (MDA) were monitored and analyzed before and after treatment.Results The levels of SOD,MDA were (51.08 ± 7.80),(75.09 ± 8.03) μ U/L and (7.13 ± 0.89),(4.51 ±0.61 ) μ mol/L before and after treatment in the treatment group,and (52.12 ± 7.31 ),(65.16 ± 8.01 ) μ U/Land (7.11 ± 0.87),(6.21 ± 0.78 ) μ mol/L in the control group.There were significant differences before and after treatment in two groups (P＜0.01 or ＜0.05),and there were significant differences after treatment between two groups (P ＜ 0.05).The levels of IL-6,IL-8 and TNF- α were significant differences before and after treatment in the treatment group [ ( 18.25 ± 7.24) ng/L vs.(29.02 ± 6.70) Ng/L,( 115.28 ± 13.76 ) ng/Lvs.(148.99 ± 16.61) ng/L,(20.43 ±3.92) ng/L vs.(32.32 ± 8.18)ng/L] (P ＜0.05).But there was nosignificant difference before and aftertreatment in the control group [ (25.25 ± 7.55 )ng/L vs.(28.82 ± 7.54)ng/L,( 136.27 ± 12.97) ng/L vs.( 150.21 ± 17.52) ng/L,(28.43 ± 3.92) ng/L vs.(32.56 ± 8.78) ng/L] (P＞0.05),there were significant differences after treatment between two groups (P ＜0.05).Conclusions Acetylcysteine can inhibit inflammation and e hminate free radicals in plasma.Acetylcysteine has beneficial effects on COPD.

Objective To compare the effects of different methods of getting out of bed for the first time in patients receiving lumbar disc herniation operations.Methods Totally 60 patients receiving lumbar disc herniation operations were divided into the experimental group and the control group with 30 patients in each group.The patients in the experimental group got out of bed with prone position.The patients in the control group got out of bed with lateral position.The pain scores and degrees of comfort were recorded.Results The experimental group scored 5.4±1.3 in pain degree,and the control group scored 6.1±1.8,t=0.37,P ＞ 0.05,the difference was not significantly different.The experimental group scored 85.7±10.9 in comfort degree,and the control group scored 70.6±11.5,t=4.21,P ＜ 0.05,the difference was significantly different.Conclusions The method of getting out of bed with prone position can reduce negative emotions of patients receiving lumbar disc herniation operations and improve degrees of comfort.

BACKGROUND:Previous studies have shown that continuous administration of 1×107 of bone marrow mesenchymal stem cel s can induce immune tolerance in rats undergoing kidney transplantation, but it is not yet found clinical y that curcumin exerts an on immunomodulatory effect on kidney transplantation.
OBJECTIVE:To investigate the effects of different concentrations of curcumin on bone marrow mesenchymal stem cel s-induced immune tolerance in rats after kidney transplantation.
METHODS:Rat model of kidney transplantation was made, and rat models were randomly divided into four groups:transplantation group with no treatment;bone marrow mesenchymal stem cel s group (cel group) injected with 1×107/kg bone marrow mesenchymal stem cel s via the left iliac vein (before peritoneal suture) and tail vein (from the 2nd day) for 10 days;bone marrow mesenchymal stem cel s+low/high dosage of curcumin groups (low/high dosage curcumin groups) injected intragastrical y with 2 or 10 mg/kg curcumin combined with injection of bone marrow mesenchymal stem cel s for 10 days. Transforming growth factor-β1 protein expression in the kidney tissues was examined by immunohistochemical staining. The concentrations of interleukin-2 and interleukin-6 in serum were detected by ELISA assay.
RESULTS AND CONCLUSION:After kidney transplantation, the protein expression of transforming growth factor-β1 in renal tubular epithelial cel s and renal interstitial cel s as wel as the concentrations of interleukin-2and interleukin-6 in serum were significantly higher in the transplantation group than the other groups (P<0.05). Compared with the cel transplantation group, the protein expression of transforming growth factor-β1 as wel as the concentrations of interleukin-2 and interleukin-6 reduced significantly in the low/high dosage curcumin groups (P<0.05). These findings indicate that simultaneous administration of curcumin and bone marrow mesenchymal stem cel s can effectively inhibit immune rejection reaction and improve renal function in rats after kidney transplantation.

Objective To investigate the value of laparoscopy in comprehensive early ovarian cancer staging by comparing the feasibility and safety of laparoscopy and laparotomy in surgical staging of early-stage epithelial ovarian cancer (EOC). Methods A total of 102 patients with EOC who underwent comprehensive laparoscopic (LPS group, n=71) or laparotomic (LPT group, n=31) staging at Southwest Hospital from November 2007 to November 2014 were retrospectively analyzed. The perioperative parameters, postoperative complication rate and the long-term curative effect were compared between the two groups. Results (1)LPS group had less intra-operative blood loss [(288±239) vs (631±463) ml], lower rate of blood transfusion(14%vs 58%), larger number of pelvic dissected lymph nodes (18.1±5.6 vs 15.5± 4.6), lower vasual analogue scalescore(VAS) pain score (2.1 ± 1.6 vs 3.0 ± 1.1), shorter gastrointestinal recovery time [(2.6 ± 0.8) vs (3.5 ± 0.9) days] and shorter hospital stay [(9.9 ± 2.9) vs (11.3 ± 5.0) days] when compared with LPT group (all P<0.01). No significant difference were found in operation time, number of para-aortic lymph nodes, rate of postoperative upstaging and adjuvant chemotherapy between the two groups (all P>0.05). (2) No significant difference was found in postoperative rate of complications [11%(8/71) vs 19%(6/31),χ2=1.192, P=0.275]. (3) No significant difference was found in recurrence rate [17%(11/66) vs 14%(4/29), χ2=0.125, P=0.724] and 5-year overall survival (86.7% vs 86.8%, P=0.874). Conclusion Compared with LPT group, there are no significance differences in recurrence rate and mortality between two groups, laparoscopic staging, which could be recommended as a choice of surgical treatment of early ovarian cancer, shows more favorable operative outcomes including minimally invasive, less intra-operative blood loss, less postoperative pain and quicker recovery.

Objective To explore the establishment and evaluation of various forms of complementary transfer. Methods The former method using the traditional handover method, the establishment of various forms of complementary shift model improved, including optimized bedside handover, handover of patients′ bedsore image and emotional barometer over emotional changes, establish WeChat group to observe improvement;the first two months (99 cases, monitoring 42 days) and the improvement after the six months (97 cases, monitoring 43 days) score of nurses for the patient to grasp, bedside shift time and transfer the missing information. Concrete research and implementation methods, research objects, sample size, measurement indicators, evaluation methods, etc. Results Both shift mode, there is no information on patients admitted to general statistical difference (P>0.05);under complementary shift mode, nurse the patients′ condition from the master score (80.95 ± 4.30) points increased to (84.88 ± 4.01) points, in which the patients′condition data mastery score from (40.81 ± 2.02) points increased to (44.16 ± 2.88) points, nursing problems and measures of control points value from (13.21 ± 1.54) points increased to (14.44 ± 1.72) points, the difference was statistically significant (t =4.362, 6.209, 3.462, P<0.01);general information master score, treatment information at its disposal no difference scores statistically significant (P>0.05). Bedside nurse shift change time decreased from former improvements (31.21 ± 2.63) minutes to (14.42 ± 2.40) minutes, and the difference was statistically significant (t=30.767, P=0.000). Shift nurse missing information dropped from 16 times to 7 times, the difference was statistically significant (χ2=5.124, P=0.024). Conclusions Complementary model can reduce the insufficiency of current clinical succession work, help nurse master the patients′physical and mental conditions and more comprehensive understanding of the ward, reduce the omission or missing information and improve the working efficiency and responsibility.

Objective To study the application effect of multi-dimensional health education model in the outpatient department of gynecological painless surgery,and explore more standardized and more effective education method.Methods 2 639 patients were selected in Sir Run Run Shaw Hospital outpatient service of gynecological department from July 2010 to June 2011,and were randomly divided into the control group(1 302 cases) and the intervention group(1 337 cases).The control group adopted conventional health education,the intervention group adopted the multi-dimensional health education.Design form was used to analyze preoperative compliance of the two groups.Results The preoperative compliance of the intervention group was higher than those of the control group,the difference was statistically significant.Conclusions Multi-dimensional health education mode can improve the preoperative education compliance of patients in the outpatient service of department of gynecological painless surgery patients,reduce the rate of operation delay and anesthesia method alteration,reduce patient pain,increase peri-operative nursing quality of painless surgery.