Sphingosine-1-phosphate receptor 1(S1PR1) is a new member of G protein-coupled receptor family. A great body of data suggest S1PR1 is capable of regulating lots of downstream signaling molecules and cellular processes. It is found that S1P/S1PR1 plays an important role in the development and mainte-nance of pain. However, it is controversial whether activation of S1PR1 would enhance or attenuate pain. Here, recent studies ＜br> and current perspectives are discussed in order to better under-stand the biological and pathological roles of S1PR1 in pain mod-ulation.

Objective To understand the status of health knowledge and behaviors on clonorchiasis sinensis of urban resi-dents in Yanqing District of Beijing City,China,so as to provide the targeted suggestions for further health education. Meth-ods The communities/streets were selected as the investigation sites by using the stratified randomly sampling method. A total of 283 residents were randomly selected and investigated with the structural questionnaire designed by Beijing Center for Dis-ease Control and Prevention. The fecal samples were collected and the eggs of Clonorchis sinensis were detected with Kato-Katz technique. Results All of the questionnaires were valid. The awareness rate about clonorchiasis was 25.09％. The rates of know-ing clonorchiasis infection route,harm and prevention measures were 10.60％,9.89％and 10.60％respectively. Totally 7.07％of the residents had the habits of eating raw or undercooked fresh water fish,and 2.47％of the residents had the behavior of eat-ing sashimi. C. sinensis eggs were not found among the research objects. Conclusions The awareness rate about clonorchiasis is low among urban residents in Yanqing District. The focus of the future work is to extensively carry out the health education about clonorchiasis prevention and control and take relevant interventions.

Objective To find out the difference between fetal malnutrition (FM) and small for gestational age (SGA) and its clinical significance. Methods Clinical nutritional status was assessed in 548 singleton term babies. Nine superficial, rapidly detected signs of malnutrition were taken for the clinical assessment nutritional status score (CANSCORE). FM was diagnosed if the total score was less than or equal to 24. Results Among 40 SGA, 21(52.5%) were FM, the other 19 (47.5%) were not FM with scores more than 24, whereas 13(2.8%) out of 508 AGA (appropriate for gestational age) were FM. Conclusion SGA and FM are not synonymous and FM can be rapidly determined by the CANSCORE. Biochemical and ultrasonic studies should be done in high risk preg nancy during second trimester to discover FM and intervene by nutritional treatment to prevent the infants with FM.

Objective To investigate the safety and effectiveness of high intensity focused ultrasound (HIFU ) in the treat‐ment of uterine fibroid (<4 cm) .Methods Retrospectively studied 79 single fibroid (<4 cm) patients that treated with HIFU in our hospital from January 2011 to February 2013 .Evaluated the improvement of post‐treatment ,shrinkage of fibroid volume and HIFU‐related adverse reaction .Results All the 79 patients were successfully cured by HIFU treatment .The shrinkage rate of fi‐broid was 79 .0% (four point spacing 57 .9% ,92 .1% ) after 2‐year treatment ;Symptom severity score (SSS) and uterine fibroid symptom and quality of life score (UFS‐QOL) were significantly improved ;and there was no adverse reaction .Conclusion HIFU can be safely used in the treatment of small uterine fibroids (diameter<4 cm ) .

Objective To summarize the clinical features of pituitary carcinoma and discuss the diagnosis,treatment and prognosis.Methods Clinical manifestations,imaging,pathologic features of one case of pituitary carcinoma were analyzed and literatures about diagnosis,treatment and prognosis of pituitary carcinoma were reviewed.Results The patient manifested polyuria onset,headache and anterior pituitary dysfunction and MRI showed pituitary lesions.He had been undergone two transsphenoidal surgery in our hospital,pathologic examinations were respectively non-functioning adenomas and atypical pituitary adenoma and tumor proliferation index (Ki-67) both were more than 30％.He was recommended radiation therapy but refused.Five months later,he was diagnosed as primary pituitary gland cancer due to the tumor widespread intracranial metastasis and no other malignant lesions in body.Conclusions Primary pituitary carcinoma is a very rare tumor,mostly transformed from the invasive pituitary adenoma.It helps predict tumor proliferation and prognosis to test the level of Ki-67 index.Primary pituitary adenocarcinoma needs comprehensive treatment and the prognosis is related to the treatment response.

Objective To investigate the effects of 20% lipid emulsion on plasma ropivacaine concentration and myocardial ropivacaine content in rats. Methods Sixty male pathogen-free SD rats weighing 220-270 g were randomly divided into 2 groups (n = 30 each): group A normal saline and group B lipid emulsion.The animals were anesthetized with intraperitoneal 4% pentobarbital 40 mg/kg. The femoral vein was cannulated for drug and fluid administration. ECG (lead Ⅱ) was continuously monitored. 1% ropivacainc 5 mg/kg was injected iv. A bolus of 20% lipid emulsion 5 ml/kg was then injected iv in group B, while in group A equal volume of normal saline was administered instead of 20% lipid emulsion. The animals were sacrificed at 5, 10,20, 40, 60 and 120 min after ropivacaine infusion (5 animals at each time point). Blood samples and myocardial specimens were taken for determination of plasma and myocardial ropivacaine levels by HPLC. Results Plasma ropivacaine concentration at 20 min after ropivacaine administration was significantly higher in group B than in group A. The myocardial ropivacaine concents at 5, 10 min after ropivacaine administration were significantly lower in group B than in group A. Conclusion 20% lipid emulsion infusion can bind ropivacaine and decreasee myocardial ropivacaine content thus reducing the cardiac toxicity of ropivacaine.

BACKGROUND: Various types of collagen affect differentiation and expression of chondrocytes in the process of tissue engineered culture for cartilage. The reliability of cartilage tissue engineering is determined by resource, structure and property of collagen.OBJECTIVE: To characterizing the structure of collagen type Ⅰ,Ⅱ and Ⅲ, to clarify the structural characteristics of collagen for cartilage tissue repairing and provide guidance for choosing cartilage tissue engineering scaffolds. METHODS: The type Ⅰ and Ⅲ collagen was obtained from neonatal calf skins, and type Ⅱ collagen was obtained from porcine articular cartilage. The differences of structures of collagen type Ⅰ,Ⅱ and Ⅲ were analyzed and compared by FTIR, DSC, SDS-PAGE and picrosirius-red staining.RESULTS AND CONCLUSION: The neonatal calf collagen type Ⅰ,Ⅱ and porcine collagen type Ⅱ had the similar functional group. The thermal denaturation temperature of the three type collagen was 104.2 ℃, 99.7 ℃ and 92.5 ℃. SDS-PAGE analysis showed the collagen type Ⅲ percentage in collagen type Ⅰ was (11.29±0.91)%. Picrosirius-red staining exhibited that the collagen type Ⅰ was comprised red and orange raw fibers, with strongly birefringent. Collagen type Ⅲ was lax and thin, greenish fibers, but collagen type Ⅱ was red, loosely cancellated fibers. The functional groups and important characteristics of the three of collagen are similar. However, there are some differences in advanced structure. To analyze the relationships of collagen type Ⅰ,Ⅱ and Ⅲ is important to choose and prepare scaffolds for cartilage tissue engineering.

BACKGROUND:Acupuncture has been found to be effective for alleviating low back pain and acute pain due to knee arthritis, but its effect on chronic pain is under discussion. OBJECTIVE:To investigate the mechanism underlying electroacupuncture (EA) alleviating chronic pain in a New Zealand rabbit model of knee arthritis. METHODS:(1) Thirty-two New Zealand rabbits were selected, and the knee osteoarthritis model was established by injecting 4% papain into the knee articular cavity of rabbit's bilateral hind limbs. The model rabbits were randomly divided into four groups (n=8 per group): normal saline plus EA, normal saline plus sham EA, nor-Binaltorphimine (nor-BNI) plus EA, and nor-BNI plus sham EA groups. The dosage of nor-BNI was 1 mg/kg, once daily, for consecutive 3 days. 30-minute EA was given at 2 hours after administration, once daily, until the day the rabbits were killed. Sham EA indicated no given electric current. The behaviors of the lower limbs were evaluated by Basso, Beattie, Bresnahan scores. The rabbits were respectively killed at 1, 3, 5 and 7 days after administration, the spinal cord was separated, and then fixed with formaldehyde. The expression levels of interleukin-17, interleukin-17 receptor A and NR1 in the spinal cord tissues were detected by immunofluorescence. (2) The other 24 New Zealand rabbits were randomly divided into model and control groups (n=12 per group), and the knee osteoarthritis model was established in the former group. Afterwards, the two groups were randomized into two subgroups, followed by given the intrathecal administration of normal saline, or 2 μg interleukin-17 antibody serum dissolvedin 10 μL normal saline, once daily, for consecutive 3 days. The behaviors of the lower limbs were evaluated by Basso, Beattie, Bresnahan scores, and the expression levels of p-NR1 and interleukin-17 receptor were detected by western blot assy. RESULTS AND CONCLUSION:The Basso, Beattie, Bresnahan scores in the nor-BNI plus EA group were significantly increased, while the expression levels of interleukin-17, interleukin-17 receptor A and NR1 in the spinal cord tissues were significantly decreased (P< 0.05). The expression level of NRI did not differ significantly between nor-BNI plus EA and nor-BNI plus sham EA groups (P> 0.05). After administration of interleukin-17 antibody serum, the Basso, Beattie, Bresnahan scores in the model group was significantly increased, and the expression levels of interleukin-17 and NR1 in the spinal cord tissues were significantly decreased, but still significantly higher than those in the control subgroups (P< 0.05). These results suggest that chronic pain in knee arthritis is the result of an increase in the expression level of NRI induced by interleukin-17. EA can remarkably improve the pain in the model rabbits of knee arthritis by downregulating interleukin-17 in the spinal cord tissues, rather than interleukin-17 receptor.

BACKGROUND:Heat shock proteins have unique biological characteristics, and exploration of such proteins expressed in the myocardium under exercise stress has important value.
OBJECTIVE:To investigate the studies about the expression of myocardial heat shock proteins under stress condition, and analyze the expression characteristics of myocardial heat shock proteins under different stress conditions.
METHODA computer-based online retrieval was performed to find articles about the production, classification and function of heat shock proteins, as wel as the expression of myocardial heat shock proteins published from January 1991 to January 2014 in PubMed database and Wanfang databases. The key words were“heat shock protein;myocardium;exercise stress”in English and Chinese. Final y 48 relevant articles were summarized.
RESULTS AND CONCLUSION:Heat shock proteins have the immune synergy effect. Researches show that, exercise training triggers the expression of myocardial heat shock protein. Acute exercise stress leads to a variety of physiological and biochemical changes, accordingly myocardial heat shock protein wil make the corresponding expression and protect myocardial cells. Low-intensity exercise can increase the expression level of heat shock protein 72, and inhibit cardiomyocyte apoptosis. High-intensity exercise reduces the expression of heat shock protein 72, which can not effectively inhibit cardiomyocyte apoptosis and is not conducive to the myocardial protective effect. The expression of heat shock protein under moderate-intensity exercise remains controversial. Exercise-induced expression of heat shock protein may have protection effect against damage induced by exercise, moderate exercise activities play an important role in enhancing myocardial function and preventing myocardial injury.

Objective: To determine the expression of von Willebrand factor Cand epidermal growth factor domains (VWCE ) gene in human breastcancer tissues, and to assess the effects of VWCE overexpression onproliferation and migration of breast cancer cells in vitro .Methods: The expression levels of VWCE mRNA and protein in 36pairs of human breast cancer tissues and their adjacent normal tissues as well as 5 kinds of breast cancer cell lines were detected by real-time fluorescentquantitative PCR and Western blotting, respectively. The recombinant lentivirus carryingVWCE gene was constructed and infected into breast cancer MCF-7 and SK-BR-3 cells. Afterthe overexpression of VWCE gene was verified by real-time fluorescent quantitative PCR andWestern blotting, the proliferation and migration of breast cancer cells were detected byCCK-8 and Transwell chamber assay, respectively.Results: The high expression rate of VWCE mRNA in breast cancer tissues was significantlylower than that in the corresponding adjacent tissues (22% vs 78%, P < 0.05), and wasrelated with the primary tumor stage (P < 0.05). The mean expression level of VWCE proteinin breast cancer tissues was lower than that in the corresponding adjacent tissues (P < 0.05).The expression levels of VWCE mRNA and protein in 5 kinds of breast cancer cell lines werelower than those in immortalized mammary epithelial cell line (all P < 0.05), respectively.After the breast cancer MCF-7 and SK-BR-3 cells were infected with VWCE overexpressionlentivirus, the expression of VWCE protein was significantly increased (both P < 0.05), whilethe cell proliferation and migration were significantly inhibited (all P < 0.05).Conclusion: VWCE is low expressed in human breast cancer tissues and cells. Overexpressionof VWCE can inhibit the proliferation and migration of breast cancer cells in vitro .