Objective:To investigate the pharmacokinetics of probucol(PB)self-microemulsifying drug delivery system(PB-SMEDDS) in rats.Methods:Rats were used as the models.With PB suspension as control,double cycle crossover with single oral dose was performed to investigate the pharmacokinetics of PB-SMEDDS.The HPLC method was established to detect drug concentration in the rat plasma,and the obtained data were processed with 3P97 software.Results:The plasma concentration-time profiles of PB-SMEDDS conformed to a two-compartment model(the coefficient weight is 1/C2).Cmax,tmax and AUC0～96h were 6.72?g/ml,8.58 h and 253.98(?g.h)/ml,respectively.The AUC0～96h of PB-SMEDDS was 19.08 times of PB suspension.Conclusion:PB-SMEDDS could improve the oral bioavailability of PB significantly.

Agonists of peroxisome proliferator-activated receptor gamma (PPARgamma) are of interest as a treatment of type II diabetes, and indenone derivatives are a new class of non-TZD PPARgamma agonists. Based on existing indenone derivatives, a series of novel ones have been designed and synthesized. Meanwhile the structures have been comfirmed with 1H NMR and MS. Among them, 17b and 19 showed higher agonistic activities than rosiglitazone.

Objective To explore the mechanism of combined treatment with methotrexate (MTX) and Ga-Al-As laser irradiation for rheumatoid arthritis (RA) and to assess the effectiveness of Ga-Al-As laser therapy for RA. Methods Twenty-two patients with RA were randomly and evenly divided into two groups: the treatment group treated with Ga-Al-As laser irradiation combined with MTX and the control group treated with MTX only. Ten age-matched normal subjects were observed as normal controls. The amount of CD4 + CD25 + regulatory T cells in peripheral blood (PB) of the normal controls and that in the PB and synovial fluid (SF) of the 22 patients before and after therapy were counted by flow cytometry. Meanwhile, the amount of prostaglandin E2 (PGE2) in synovial fluid of the patients was measured before and after treatment by enzyme-linked immunosorbent assay(ELISA). Results After combined treatment the clinical symptoms of the patients were improved significantly, and the amount of PGE2in SF decreased significantly. The count of CD4 + CD25 + regulatory T cells in PB of RA patients was ( 3.84 ±3.20) % , compared to ( 10.05 ± 7.04) % in healthy individuals. The count of CD4 + CD25 + regulatory T cells in SF of RA patients was ( 14.89 ± 12.30) % , much higher than that in PB. The count of CD4 + CD25 + regulatory T cells in SF decreased significantly in treatment group compared to control group (P ＜0.05). Conclusion Ga-Al-As laser irradiation eombined with MTX can effectively improve the clinical symptoms of RA patients. It may be related to the decrease of amount of PGE2 and count of CD4 + CD25 + regulatory T cell in PB and SF.

Prevention of infection by the human papillomavirus (HPV) has become a hot research topic since the relationship between the HPV and cervical cancer was confirmed. Persistent infection with HPV and early expression of proteins has an important role in the pathogenesis of cervical cancer. Vaccines that protect against four high-risk types of HPV (-6, -11, -16, -18) have been used worldwide. A bivalent vaccine (HPV-16 and -18) developed by Walvax is in clinical trials. This study reviews progress in ascertainment of the structure and function of the HPV genome, the molecular mechanism of carcinogenesis, and vaccines based on virus-like particles.
Animals ; Carcinogenesis ; Female ; Humans ; Papillomaviridae ; genetics ; immunology ; metabolism ; Papillomavirus Infections ; pathology ; prevention & control ; virology ; Papillomavirus Vaccines ; genetics ; immunology ; Uterine Cervical Neoplasms ; pathology ; prevention & control ; virology ; Viral Proteins ; genetics ; immunology ; metabolism

Objective To establish a three-dimensional digitized visible model to meet the clinical and research needs.Methods The head of the volunteer was scanned by a cone beam CT (CBCT).The bone tissue,the facial soft tissue and the teeth were extracted from the cone beam CT raw data according to the different Houns field values.The 3D digital craniofacial model was reconstructed from the three tissues extracted from the CBCT original data.The 3D dental model with high resolution was acquired by scanning the dental cast with the laser scanner.The 3D facial model with texture was acquired by scanning the volunteer's face directly by other laser scanner.With the regis tration algorithm in 3D graphics,the 3D facial model with texture and the 3D dental model with high resolution were transformed to the same coordinate which was of the 3D digital craniofacial model.Results We acquired a complicated 3D craniofacial digital model which combined the teeth model with high resolution,the bone model and the face model with texture.Conclusions The method is able to accurately establish the craniofacial digital model.

ObjectiveTo investigate the effect of moxibustion on the Governor Vessel in combination with acupuncture on ankylosing spondylitis.MethodsThirtypatetints with ankylosing spondylitis(kidneyyang deficiency pattern) were recruited and treated with moxibustion on the Governor Vessel in combination with acupuncture. The scores of Scale of Quality of Life for Ankylosing Spondylitis (SQOL-AS), Bath Ankylosing Spondylitis Disease Activity Index(BASDAI), Bath Ankylosing Spondylitis Functional Index(BASFI), Fatigue Assessment Instrument(FAI)and erythrocyte sedimentation rate(ESR) before and after treatment were evaluated.ResultsThe scores of BASDAI(0.427±0.381vs. 6.107±1.375;t=28.986,P<0.01), BASFI(0.753±0.504 vs. 7.480±1.152;t=36.580,P<0.01), FAI(40.067±14.746vs. 146.967±26.283;t=24.520,P<0.01)after treatment were significantly lower than those before treatment; ESR after treatment were significantly lower than that before treatment(19.833±13.929 mm/hvs. 31.567± 20.065 mm/h;t=3.708,P<0.01). The subscores of Physical Functioning(33.000±1.930vs. 12.033±1.790;t=62.165,P<0.01), Mental Health(25.367±2.059vs. 14.067±2.303;t=30.139,P<0.01), Social Functioning(21.267±4.025vs. 8.767±1.977;t=26.643,P<0.01) and General Health Perception (22.733± 2.690vs. 9.767±2.738;t=25.729,P<0.01) of SQOL-AS after treatment were significantly lower than that before treatment.ConclusionsMoxibustion on the Governor Vessel in combination with acupuncture can improve physical functioning and reduce disease activity in patients with ankylosing spondylitis.

Objective]Observe clinical efficacy of herpes zoster treated by the compound radix scutellariae solution wet application. [Method]50 patients were randomly divided into 25 cases of the compound radix scutellariae group and 25 cases of the nitrofurazone group, respectively given wet application of the compound radix scute-llariae solution and nitrofurazone solution, at the same time given intravenousganciclovir 0.3g/time,1 time/d, oral vitamin B1 20mg/time, 3 times/d, oral cobalt amine 0.5mg/time, 3 times/d, one week as a period. Record all patients' pain, skin lesion scores before the treatment and in the course of the third, fifth, seventh day and record the time of herpes stopping hair, blister drying up, blister beginning to scab and the scabby area not less than 50%.[Result] Pain, skin lesion scores of the compound radix scutellariae group were signifycantly lower than those of nitrofurazone group, the time of herpes stopping hair, blister drying up, blister beginning to scab and the scabby area not less than 50% of the compound radix scutellariae group compared with nitrofurazone group in advance, the difference was statistically significant(P<0.05). [Conclusion]Treatment of the compound radix scutellariae solution to herpes zoster had distinct clinical efficacy, having significant advantages in terms of alleviating pain and promoting skin lesions subsided.

Objective To analyze the efficacy of umbilical one trocar laparoscopic appendectomy (UOTLA) in treatment of complicated appendicitis in children. Methods Clinical data of 78 cases of children patients with complicated appendicitis from January 2012 to October 2015 was summarized, including 44 cases as UOTLA group received umbilical one trocar laparoscopic appendectomy, 34 cases as OA group received open appendectomy. Then statistically analyzed all the patients' operation time, postoperative hospital stay, postoperative abdominal abscess, incision infection, early inflammatory intestinal obstruction and pain level. Results The laboratory test results of C reaction protein (CRP) had no significant difference between the two groups, while peripheral white blood cell count decreased more significantly in UOTLA group than that in OA group; the operation time of UOTLA group was shorter than that in OA group with no statistical difference [(66.59 ± 33.24) vs (72.86 ± 30.36) min, P > 0.05], but postoperative hospital stay was shorter [(8.21 ± 1.67) vs (9.21 ± 2.01) d, P < 0.05]. Abdominal abscess after operation: 3 cases in UOTLA group, while 1 case in OA group (P > 0.05); incision infection: 6 cases in UOTLA group, 9 cases in OA group (P > 0.05); early inflammatory intestinal obstruction: 1 cases in UOTLA group, 5 cases in OA group (P > 0.05); the pain level, postoperative recovery time was significantly shorter in UOTLA group compared with OA group (P < 0.05). The average expenses comparison of the two groups has no statistical difference [(10639.37 ± 2970.92) vs (10765.04 ± 2902.64) yuan, P > 0.05]. Conclusion UOTLA is safe and effective for complicated appendicitis in children due to minimally invasive, less pain and faster recovery without significant increase in the cost and postoperative complications. It can be applied in children with purulent, perforated appendicitis and gangrene, perforated appendicitis and other complicated appendicitis.

Objective To discusse the effect of edaravone for brain tissue oxidative damage after cerebral hemorrhage through observing the hemorrhage rats back edema and the change of lipid peroxidation.Methods 150 healthy and clean male SD rats, were randomly divided to sham operation group,control group,treatment group,50 cases in each group,each group choose five time points,post operative 0.5 day,1 day,2 days,3 days, 4 days,observed different 10 rats at each time point.The intracerebral hemorrhage model was made by using the method of stereotactic autologous blood injection.The changes of water content, the content of MDA and the activity of superoxide dismutase were monitored in three groups at different time points.Results Surrounding the hematoma in the brain tissue around the hematoma after intracerebral hemorrhage in sham operation group,treatment group of postoperative day 1-4 days each time hematoma surrounding brain tissue water content was significantly lower than that in control group(P<0.05);Control group after 2 days of cerebral edema and reached the highest value;Sham operation group and treatment group of postoperative half of the day to 4 days each time the content of MDA were significantly lower than in control group ( P <0.05 );Sham operation group and treatment group postoperative half of the day to 4 days each time the brain tissue SOD activity was significantly higher than control group(P<0.05).Conclusion The effect is obvious that edaravone for cerebral hemorrhage rats brain tissue around hematoma edema, reduce cerebral hemorrhage rat tissue oxidative damage.